Download or read book Expression of the Galactose Metabolism Genes in Yeast written by Bruce Alexander Citron and published by . This book was released on 1982 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Galactose Regulon of Yeast written by Paike Jayadeva Bhat and published by Springer Science & Business Media. This book was released on 2008-03-02 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: This self contained book presents a comprehensive overview of the past, present and future of the galactose regulon of yeast, the classical model system of molecular biologists. The book starts with a brief historical overview on yeast research. This is followed by molecular genetics of the galactose regulon, isolation of genes and testing of the hypotheses. Contemporary topics including genomics, evolution, binary and graded responses, and stochasticity are all addressed.
Download or read book Yeast Sugar Metabolism written by Friedrich K. Zimmermann and published by CRC Press. This book was released on 1997-03-10 with total page 590 pages. Available in PDF, EPUB and Kindle. Book excerpt: Yeast Sugar Metabolism looks at the biomechanics, genetics, biotechnology and applications of yeast sugar. The yeast Saccharomyces cereisiae has played a central role in the evolution of microbiology biochemistry and genetics, in addition to its use of a technical microbe for the production of alcoholic beverages and leavening of dough.
Download or read book Molecular Mechanisms in Yeast Carbon Metabolism written by Jure Piškur and published by Springer. This book was released on 2014-05-06 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: Yeast is one of the most studied laboratory organisms and represents one of the most central models to understand how any eukaryote cell works. On the other hand, yeast fermentations have for millennia provided us with a variety of biotech products, like wine, beer, vitamins, and recently also with pharmaceutically active heterologous products and biofuels. A central biochemical activity in the yeast cell is the metabolism of carbon compounds, providing energy for the whole cell, and precursors for any of the final fermentation products. A complex set of genes and regulatory pathways controls the metabolism of carbon compounds, from nutrient sensing, signal transduction, transcription regulation and post-transcriptional events. Recent advances in comparative genomics and development of post-genomic tools have provided further insights into the network of genes and enzymes, and molecular mechanisms which are responsible for a balanced metabolism of carbon compounds in the yeast cell, and which could be manipulated in the laboratory to increase the yield and quality of yeast biotech products. This book provides a dozen of most comprehensive reviews on the recent developments and achievements in the field of yeast carbon metabolism, from academic studies on gene expression to biotechnology relevant topics.
Download or read book Yeast Metabolic Engineering written by Valeria Mapelli and published by Humana Press. This book was released on 2014-04-18 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume. Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies.
Download or read book The Molecular Biology of the Yeast Saccharomyces written by Jeffrey N. Strathern and published by . This book was released on 1982 with total page 730 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic and Molecular Dissection of the Integration of Galactose and Glucose Signaling in Saccharomyces Cerevisiae Strains written by Renan Antonio Escalante Chong and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells need to sense the environment in order to survive, in particular they need to detect nutrients which will provide different building blocks and energy for the cell. This task is complicated by the fact that there can be multiple sources for the same type of nutrient available for the cell. A classical example of how cells sense multiple signals is given by carbon catabolite repression in the budding yeast S. cerevisiae. In this model the preferred carbon source glucose represses the genes used to metabolize an alternative source such as galactose. This means that the preferred carbohydrate glucose is thought to inhibit the induction of galactose genes when above a threshold concentration. Instead, we show that galactose metabolic genes (GAL) induction depends on the ratio of galactose and glucose. Surprisingly, we find that a critical portion of information processing occurs upstream of the canonical components of the GAL pathway. We then explore how cells choose between different responses to the environment. Specifically, we set out to characterize the variability in the response to combinations of galactose and glucose between several natural yeast isolates. To elucidate the genetic basis of this phenotypic variation we use QTL mapping on these strains. Our study reveals that a signal transducer GAL3 plays a central role in establishing variation in GAL gene induction.Lastly, we focus on the control of transcription in the cell. Many promoters in the cell produce both a coding transcript and a divergent transcript. To identify mutants that affect transcriptional directionality we use a bidirectionalfluorescent protein reporter in the yeast nonessential gene deletion collection. We determine that chromatin assembly can regulate divergent transcription. Moreover, mutations in the chromatin assembly factor CAF-I can lead to genome wide derepression of nascent divergent transcription.
Download or read book Control of Macromolecular Synthesis written by Ole Maaløe and published by . This book was released on 1966 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Evolutionary Constraints and Dynamics of Sugar Metabolism in Yeasts written by Meihua Kuang and published by . This book was released on 2017 with total page 115 pages. Available in PDF, EPUB and Kindle. Book excerpt: Our incomplete understanding of the relationship between genotypes and phenotypes makes it extremely difficult to understand and predict the evolution of diverse phenotypes in nature. Phenotypes evolve on the basis of genetic alterations. Gene regulatory networks (GRNs) build the link between genotypes and phenotypes. Using well-characterized GRNs as models may get us closer to understand the relationship between genotypes and phenotypes. Taking advantage of powerful yeast genetics, this dissertation uses the yeast GALactose (GAL) utilization networks as a model and carefully dissects three levels of evolutionary mechanisms between genotypes and phenotypes. The three levels include the functional divergence of individual genes, divergence of GRN activities, and the co-evolution between individual genes and between interacting GRNs. Through these three levels of characterization, I reveal that GAL network activity is constrained by downstream glycolysis capacity. This is likely a general constraint to sugar metabolized through glycolysis. I further examine recurring genetic changes underlying repeated tuning of GAL network activities across multiple genera and reveal a general pleiotropic constraint at the bottleneck of galactose metabolism. These constraints even enable remarkable prediction of GAL network activities merely based on DNA sequences. Understanding the genetic and functional basis of trait divergence reveals general evolutionary constraints and allows us to predict evolutionary outcomes.
Download or read book Yeast Stress Responses written by Stefan Hohmann and published by Springer Science & Business Media. This book was released on 2007-10-23 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: Every cell has developed mechanisms to respond to changes in its environment and to adapt its growth and metabolism to unfavorable conditions. The unicellular eukaryote yeast has long proven as a particularly useful model system for the analysis of cellular stress responses, and the completion of the yeast genome sequence has only added to its power This volume comprehensively reviews both the basic features of the yeast genral stress response and the specific adapations to different stress types (nutrient depletion, osmotic and heat shock as well as salt and oxidative stress). It includes the latest findings in the field and discusses the implications for the analysis of stress response mechanisms in higher eukaryotes as well.
Download or read book Biodiversity and Ecophysiology of Yeasts written by Carlos Augusto Rosa and published by Springer Science & Business Media. This book was released on 2006-03-30 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last few decades more and more yeast habitats have been explored, spanning cold climates to tropical regions and dry deserts to rainforests. As a result, a large body of ecological data has been accumulated and the number of known yeast species has increased rapidly. This book provides an overview of the biodiversity of yeasts in different habitats. Recent advances achieved by the application of molecular biological methods in the field of yeast taxonomy and ecology are also incorporated in the book. Wherever possible, the interaction between yeasts and the surrounding environment is discussed.
Download or read book Metabolism and Molecular Physiology of Saccharomyces Cerevisiae written by J. Richard Dickinson and published by CRC Press. This book was released on 2004-04-27 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the best-selling first edition, much has been discovered about Saccharomyces cerevisiae, the single-celled fungus commonly known as baker's yeast or brewer's yeast that is the basis for much of our understanding of the molecular and cellular biology of eukaryotes. This wealth of new research data demands our attention and r
Download or read book Non conventional Yeasts from Basic Research to Application written by Andriy Sibirny and published by Springer. This book was released on 2019-08-12 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume scopes several aspects of non-conventional yeast research prepared by the leading specialists in the field. An introduction on taxonomy and systematics enhances the reader’s knowledge on yeasts beyond established ones such as Saccharomyces cerevisiae. Biotechnological approaches that involve fungal utilization of unusual substrates, production of biofuels and useful chemicals as citric acid, glutathione or erythritol are discussed. Further, strategies for metabolic engineering based on knowledge on regulation of gene expression as well as sensing and signaling pathways are presented. The book targets researchers and advanced students working in Microbiology, Microbial Biotechnology and Biochemistry.
Download or read book Next Steps for Functional Genomics written by National Academies of Sciences, Engineering, and Medicine and published by National Academies Press. This book was released on 2020-12-18 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the holy grails in biology is the ability to predict functional characteristics from an organism's genetic sequence. Despite decades of research since the first sequencing of an organism in 1995, scientists still do not understand exactly how the information in genes is converted into an organism's phenotype, its physical characteristics. Functional genomics attempts to make use of the vast wealth of data from "-omics" screens and projects to describe gene and protein functions and interactions. A February 2020 workshop was held to determine research needs to advance the field of functional genomics over the next 10-20 years. Speakers and participants discussed goals, strategies, and technical needs to allow functional genomics to contribute to the advancement of basic knowledge and its applications that would benefit society. This publication summarizes the presentations and discussions from the workshop.
Download or read book Non Conventional Yeasts in Genetics Biochemistry and Biotechnology written by Klaus Wolf and published by Springer Science & Business Media. This book was released on 2003-03-04 with total page 538 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most information on yeasts derives from experiments with the conventional yeasts Saccaromyces cerevisiae and Schizossaccharomyces pombe, the complete nuclear and mitochondrial genome of which has also been sequenced. For all other non-conventional yeasts, investigations are in progress and the rapid development of molecular techniques has allowed an insight also into a variety of non-conventional yeasts. In this bench manual, over 70 practical protocols using 15 different non-conventional yeast species and in addition several protocols of general use are described in detail. All of these experiments on the genetics, biochemistry and biotechnology of yeasts have been contributed by renowned laboratories and have been reproduced many times. The reliable protocols are thus ideally suited also for undergraduate and graduate practical courses.
Download or read book Handbook of Glycosyltransferases and Related Genes written by Naoyuki Taniguchi and published by Springer Science & Business Media. This book was released on 2011-06-28 with total page 668 pages. Available in PDF, EPUB and Kindle. Book excerpt: The so-called postgenomic research era has now been launched, and the field of gly cobiology and glycotechnology has become one of the most important areas in life science because glycosylation is the most common post-translational modification reaction of proteins in vivo. On the basis of Swiss-Prot data, over 50% proteins are known to undergo glycosylation, but in fact the actual functions of most of the sugar chains in the glycoconjugates remain unknown. The complex carbohydrate chains of glycoproteins, glycolipids, and proteoglycans represent the secondary gene products formed through the reactions of glycosyl transferases. The regulation of the biosynthesis of sugar chains is under the control of the expression of glycosyltransferases, their substrate specificity, and their local ization in specific tissue sites. There is a growing body of evidence to suggest that these enzymes play pivotal roles in a variety of important cellular differentiation and developmental events, as well as in disease processes. Over 300 glycosyltransferases appear to exist in mammalian tissues. If the genes that have been purified and cloned from various species such as humans, cattle, pigs, rats and mice are counted as one, approximately 110 glycogenes that encode glycosyltransferases and related genes have been cloned at present, and this number continues to grow each day. However, most of the functions of the glycosyltransferase genes and related genes are unknown. This fact has stimulated numerous new and interesting approaches in molecular biologi cal investigations.
Download or read book Pichia Protocols written by James M Cregg and published by Springer Science & Business Media. This book was released on 2007-08-08 with total page 553 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition.
