Download or read book Expression of Penicillin binding Protein 3 in Escherichia Coli written by Paul Martin McNicholas and published by . This book was released on 1989 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Membrane Topology of Penicillin binding Protein 3 of Escherichia Coli written by and published by . This book was released on 1990 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this thesis I present a study of the membrane topology of PBP3 of Escherichia coli and examine its suitability as a candidate for crystallisation and subsequent X-ray diffraction studies.The bacteriocidal activity of the 3-lactam antibiotics resides in their ability to bind to and inactivate a series of enzymes that mediate the final stages in peptidoglycan synthesis. There are seven of these penicillin-binding proteins (PBP's) present in Escherichia coli. The four of highest molecular weight (PBP's 1A, IB, 2 and 3) appear essential for cell growth, and since PBP3 has the highest affinity for most of the 3-lactams in current clinical use, it is, from a medical perspective, probably the most important killing target.In the first part of this thesis I present an analysis of the membrane organisation of PBP3. A series of gene fusions were constructed between the coding region of mature ITEM 3-lactamase and random positions within the PBP3 gene. The position of the fusion junction in 84 in-frame fusions was determined, with fusions to 61 different positions in PBP3 being obtained. Fusions to positions within the first 31 residues of PBP3 resulted in enzymically active fusion proteins which were unable to protect single cells of E.coli from killing by ampicillin, indicating that the 3-lactamase moieties of these fusion proteins were not translocated to the periplasm. However, all in-frame fusions containing more than 35 residues of PBP3 were able to provide single cells of E.coli with appreciable levels of resistance to ampicillin, indicating that the B-lacamase moieties of these fusion proteins were translocated to the periplasm. This data therefore implies that PBP3 has a simple membrane topology with the bulk of the protein (residues 36 to the carboxy-terminus) exposed on the periplasmic side of the inner membrane. The pattern of proteolytic sensitivity of PBP3 in both spheroplasts and membrane vesicles later confirmed this model. That PBP3 was only anchored to the inner membrane through its NHa-terminus has been shown since replacement of its putative lipoprotein signal with a normal cleaved signal peptide resulted in a water-soluble, periplasmic form of the enzyme which still bound penicillin. The last part of this thesis concerns the characterisation of this soluble PBP, and examines the possibilities for its subsequent purification.
Download or read book The Membrane Topology of Penicillin binding Protein 3 of Escherichia Coli written by Lucas David Bowler and published by . This book was released on 1990 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Molecular Genetic Analysis of the Active Centre of the Transpeptidase Domain of Penicillin binding Protein 3 of Escherichia Coli written by Karen Dorothy Cromie and published by . This book was released on 1988 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic Analysis of the Role of Penicillin binding Proteins 1A and 1B in Escherichia Coli written by S. Y. Yousif and published by . This book was released on 1986 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Penicillin binding Protein 1B of Escherichia Coli written by Caroline Anna Louise Zijderveld and published by . This book was released on 1994 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Purification and Characterization of Penicillin binding Protein 2 of Escherichia Coli written by Ora K. Dillon-Carter and published by . This book was released on 1983 with total page 54 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Penicillin binding Protein 4 of Escherichia Coli written by Harald Mottl and published by . This book was released on 1992 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bacterial Cell Wall written by J.-M. Ghuysen and published by Elsevier. This book was released on 1994-02-09 with total page 607 pages. Available in PDF, EPUB and Kindle. Book excerpt: Studies of the bacterial cell wall emerged as a new field of research in the early 1950s, and has flourished in a multitude of directions. This excellent book provides an integrated collection of contributions forming a fundamental reference for researchers and of general use to teachers, advanced students in the life sciences, and all scientists in bacterial cell wall research. Chapters include topics such as: Peptidoglycan, an essential constituent of bacterial endospores; Teichoic and teichuronic acids, lipoteichoic acids, lipoglycans, neural complex polysaccharides and several specialized proteins are frequently unique wall-associated components of Gram-positive bacteria; Bacterial cells evolving signal transduction pathways; Underlying mechanisms of bacterial resistance to antibiotics.
Download or read book Molecular Genetic Analysis of the Penicillin binding Protein 1B Gene of Escherichia Coli written by A. Edelman and published by . This book was released on 1987 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Structural and Functional Characteristics of Penicillin binding Protein 1B of Escherichia Coli as Studies by Monoclonal Antibodies written by Tanneke den Blaauwen and published by . This book was released on 1989 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zsfassung in niederländ. Sprache.
Download or read book Structural and Functional Characteristics of Penicillin binding Protein 1B of Escherichia Coli as Studied by Monoclonal Antibodies written by Tanneke den Blaauwen and published by . This book was released on 1989 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Amphipathic Helix written by Richard M. Epand and published by CRC Press. This book was released on 1993-07-09 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Amphipathic Helix is a comprehensive volume discussing amphipathic helices in systems as diverse as serum lipoproteins, lung surfactant, cytotoxic peptides, ion channels, mitochondrial targeting, peptide hormones, G proteins, T-cell recognition, DNA binding proteins, and antifreeze proteins. The book also includes general introductory material that defines amphipathic helices, discusses methods to identify amphipathic helical segments from the amino acid sequence of a protein, illustrates how amphipathic helices can be used in the de novo design of peptide and protein structures, and describes how these helices stabilize protein structures. There is also a section on techniques to determine helix orientation in a membrane environment using polarized attenuated total reflection infrared spectroscopy or solid state NMR spectroscopy. Recent developments on all these topics have been discussed by leading experts in this reference for researchers and students in biochemistry, biophysics, and pharmacology.
Download or read book Molecular Genetic Approaches to the Structure of a High Molecular Weight Penicillin binding Protein written by P. J. Hedge and published by . This book was released on 1985 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Pet to Man Travelling Staphylococci written by Vincenzo Savini and published by Academic Press. This book was released on 2018-03-14 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pet-to-Man Travelling Staphylococci: A World in Progress explores Staphylococci, a dangerous pathogen that affects both humans and animals with a wide range of infection states. This bacteria can spread rapidly as a commensal organism in both humans and pets, and is an agent of disease. Staphylococci are potentially highly virulent pathogens which require urgent medical attention. In addition, Staphylococci remain a threat within hospital environments, where they can quickly spread across a patient population. This book explores the organisms' resistance to many compounds used to treat them, treatment failure and multidrug resistant staphylococci, amongst other related topics. Focuses not only on man and animal staphylococcal diseases, but on the role of shared household in man-to-pet (and vice versa) transmission Underlines the importance of professional exposure to mammals (i.e. veterinary and farm personnel) in the establishment of shared colonization's and related diseases Highlights the impact of shared staphylococci and virulence determinants in human and veterinary pathology Sheds light on the way staphylococci may be recognized in clinical laboratories
Download or read book Antimicrobial Drug Resistance written by Douglas L. Mayers and published by Springer. This book was released on 2017-07-07 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The two volumes included in Antimicrobial Drug Resistance, Second Edition is an updated, comprehensive and multidisciplinary reference covering the area of antimicrobial drug resistance in bacteria, fungi, viruses, and parasites from basic science, clinical, and epidemiological perspectives. This newly revised compendium reviews the most current research and development on drug resistance while still providing the information in the accessible format of the first edition. The first volume, Antimicrobial Drug Resistance: Mechanisms of Drug Resistance, is dedicated to the biological basis of drug resistance and effective avenues for drug development. With the emergence of more drug-resistant organisms, the approach to dealing with the drug resistance problem must include the research of different aspects of the mechanisms of bacterial resistance and the dissemination of resistance genes as well as research utilizing new genomic information. These approaches will permit the design of novel strategies to develop new antibiotics and preserve the effectiveness of those currently available. The second volume, Antimicrobial Drug Resistance: Clinical and Epidemiological Aspects, is devoted to the clinical aspects of drug resistance. Although there is evidence that restricted use of a specific antibiotic can be followed by a decrease in drug resistance to that agent, drug resistance control is not easily achieved. Thus, the infectious diseases physician requires input from the clinical microbiologist, antimicrobial stewardship personnel, and infection control specialist to make informed choices for the effective management of various strains of drug-resistant pathogens in individual patients. This 2-volume set is an important reference for students in microbiology, infectious diseases physicians, medical students, basic scientists, drug development researchers, microbiologists, epidemiologists, and public health practitioners.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1999 with total page 1860 pages. Available in PDF, EPUB and Kindle. Book excerpt:
