Download or read book Expanding Spectral Range and Enhancing Throughput of Fluorescent Biosensor Imaging written by Clara Anne Posner and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells execute specific responses to diverse environmental cues by encoding information in distinctly compartmentalized biochemical signaling reactions. Genetically encoded fluorescent biosensors enable the spatial and temporal monitoring of signaling events in live cells. Chapter 1 of this dissertation is a reprint of work submitted for publication, that introduces the concepts of compartmentalized signaling, genetically encoded fluorescent biosensors, and temporal and spatiotemporal deterministic computational modeling and then demonstrates the power of applying computational models to interpret biosensor experiments in complex biochemical networks in two case studies. Chapter 2 of this dissertation is a reprint of published work in which we demonstrate that a mutation on a solvent-exposed residue of a red fluorescent protein (RFP) leads to a brighter, more stably fluorescent RFP. We also show that this RFP enhances green-red FRET biosensors and can be incorporated into a spectrally orthogonal FRET biosensor multiplexing scheme to investigate the crosstalk between Src, Akt, and Extracellular Regulated Kinase (ERK) kinases in single cells. Chapter 3 is a reprint of published work wherein we demonstrate that a platform combing an open-source liquid handler with a fluorescent microscope can automate and enhance throughput of fluorescent biosensor imaging experiments in response to multiple compound stimulations. We demonstrate this platform using protein kinase A (PKA) and 3',5'-cyclic adenosine monophosphate (cAMP) FRET biosensors to measure the signaling dynamics in response to varying concentrations of a single G-protein coupled receptor (GPCR) agonist, to a panel of GPCR agonists and antagonists, or to pH media exchanges. Finally, Chapter 4 is a conclusion where we summarize the findings and discuss future extensions of the work. This work describes three different methods that increase the utility of fluorescent biosensors experiments to investigate cell signaling phenomenon: quantitatively evaluating biosensor experimental data using computational models, developing a new red fluorescent protein and a three FRET biosensor multiplexing scheme, and engineering a platform that automates and increases throughput of fluorescent biosensor imaging experiments.
Download or read book Fluorescence Sensors and Biosensors written by Richard B. Thompson and published by CRC Press. This book was released on 2005-12-16 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence-based sensing is a significant technique used in prominent fields such as fluorescence-activated cell sorting, DNA sequencing, high-throughput screening, and clinical diagnostics. Fluorescence Sensors and Biosensors emphasizes the most recent developments and emerging technologies with the broadest impacts. The text begins wi
Download or read book Fluorescence Based Biosensors written by and published by Academic Press. This book was released on 2012-12-31 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the major challenges of modern biology and medicine consists in finding means to visualize biomolecules in their natural environment with the greatest level of accuracy, so as to gain insight into their properties and behaviour in a physiological and pathological setting. This has been achieved thanks to the design of novel imaging agents, in particular to fluorescent biosensors. Fluorescence Biosensors comprise a large set of tools which are useful for fundamental purposes as well as for applications in biomedicine, drug discovery and biotechnology. These tools have been designed and engineered thanks to the combined efforts of chemists and biologists over the last decade, and developed hand in hand together with imaging technologies. This volume will convey the many exciting developments the field of fluorescent biosensors and reporters has witnessed over the recent years, from concepts to applications, including chapters on the chemistry of fluorescent probes, on technologies for monitoring protein/protein interactions and technologies for imaging biosensors in cultured cells and in vivo. Other chapters are devoted to specific examples of genetically-encoded reporters, or to protein and peptide biosensors, together with examples illustrating their application to cellular and in vivo imaging, biomedical applications, drug discovery and high throughput screening. - Contributions from leading authorities - Informs and updates on all the latest developments in the field
Download or read book Metal Enhanced Fluorescence written by Chris D. Geddes and published by John Wiley & Sons. This book was released on 2010-06-22 with total page 655 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence.
Download or read book Radiative Decay Engineering written by Chris D. Geddes and published by Springer Science & Business Media. This book was released on 2007-12-11 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: During recent years our enthusiasm for this field has continually increased. This book presents expert contributions describing the fundamental principles for the widespread use of radiative decay engineering in the biological sciences and nanotechnology.
Download or read book Surface and Tip Enhanced Raman Scattering Spectroscopy written by Marek Procházka and published by Springer Nature. This book was released on with total page 744 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biosensors for Single Cell Analysis written by Jian Chen and published by Academic Press. This book was released on 2021-10-26 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biosensors for Single-Cell Analysis explores a wide range of biosensor technologies and their applications in single-cell characterization and analysis. Sections cover key biophysical and chemical single-cell properties that consider proteomic, metabolic, electrical, mechanical and optical properties. Each chapter features key definitions and case studies, providing detailed guidance for researchers who want to replicate covered solutions in their work. Tutorial sections, evaluations of the current state-of-the-field and future developments are also included. Microfluidic approaches to characterization, such as microfluidic impedance flow cytometry and microfluidic flow cytometry are considered alongside more conventional approaches, such as mass spectroscopy, fluorescent and mass flow cytometry. Additionally, key types of biosensors are covered, including atomic force microscopy, micropipette aspiration, optical tweezers, microfluidic hydrodynamic stretchers, microfluidic constriction channel and microfluidic optical stretchers. - Includes chapters focused on key single-cell properties, such as proteomic, metabolic and mechanical characterization - Features case studies that illustrate the application of biosensors for single-cell analysis - Considers microfluidic approaches for each single-cell property discussed - Explores future directions for single-cell analysis and biosensor technology
Download or read book Bioanalytics written by Friedrich Lottspeich and published by John Wiley & Sons. This book was released on 2018-03-08 with total page 1810 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology
Download or read book Chemical Exchange Saturation Transfer Imaging written by Michael T. McMahon and published by CRC Press. This book was released on 2017-01-12 with total page 463 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first textbook dedicated to CEST imaging and covers the fundamental principles of saturation transfer, key features of CEST agents that enable the production of imaging contrast, and practical aspects of preparing image-acquisition and post-processing schemes suited for in vivo applications. CEST is a powerful MRI contrast mechanism with unique features, and the rapid expansion it has seen over the past 15 years since its original discovery in 2000 has created a need for a graduate-level handbook describing all aspects of pre-clinical, translational, and clinical CEST imaging. The book provides an illustrated historical perspective by leaders at the five key sites who developed CEST imaging, from the initial saturation transfer NMR experiments performed in the 1960s in Stockholm, Sweden, described by Sture Forsén, to the work on integrating the basic principles of CEST into imaging by Robert Balaban, Dean Sherry, Silvio Aime, and Peter van Zijl in the United States and Italy. The editors, Drs. Michael T. McMahon, Assaf A. Gilad, Jeff W. M. Bulte, and Peter C. M. van Zijl, have been pioneers developing this field at the Johns Hopkins University School of Medicine and the Kennedy Krieger Institute including contributions to Nature Medicine, Nature Biotechnology, Nature Materials, and the Proceedings of the National Academy of Sciences. As recognition for their initial development of the field, Drs. van Zijl and Balaban were awarded the Laukien Prize in April 2016, established in 1999 to honor the memory of Professor Gunther Laukien, a co-founder of Bruker Biospin GmbH.
Download or read book High Throughput Screening for Food Safety Assessment written by Arun K. Bhunia and published by Elsevier. This book was released on 2014-09-06 with total page 550 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in array-based detectors and imaging technologies have provided high throughput systems that can operate within a substantially reduced timeframe and other techniques that can detect multiple contaminants at one time. These technologies are revolutionary in terms of food safety assessment in manufacturing, and will also have a significant impact on areas such as public health and food defence. This book summarizes the latest research and applications of sensor technologies for online and high throughput screening of food. The book first introduces high throughput screening strategies and technology platforms, and discusses key issues in sample collection and preparation. The subsequent chapters are then grouped into four sections: Part I reviews biorecognition techniques; Part II covers the use of optical biosensors and hyperspectral imaging in food safety assessment; Part III focuses on electrochemical and mass-based transducers; and finally Part IV deals with the application of these safety assessment technologies in specific food products, including meat and poultry, seafood, fruits and vegetables. - Summarises the latest research on sensor technologies for online and high-throughput screening of food - Covers high-throughput screening and the current and forecast state of rapid contaminant detection technologies - Looks at the use of optical and electrochemical biosensors and hyperspectral imaging in food safety assessment and the application of these technologies in specific food products
Download or read book Fluorescent Protein Based Biosensors written by Jin Zhang and published by Humana. This book was released on 2013-09-20 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Fluorescent Protein-Based Biosensors: Methods and Protocols, experts in the field have assembled a series of protocols describing several methods in which fluorescent protein-based reporters can be used to gain unique insights into the regulation of cellular signal transduction. Genetically encodable fluorescent biosensors have allowed researchers to observe biochemical processes within the endogenous cellular environment with unprecedented spatiotemporal resolution. As the number and diversity of available biosensors grows, it is increasingly important to equip researchers with an understanding of the key concepts underlying the design and application of genetically encodable fluorescent biosensors to live cell imaging. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Fluorescent Protein-Based Biosensors: Methods and Protocols promises to be a valuable resource for researchers interested in applying current biosensors to the study of biochemical processes in living cells as well as those interested in developing novel biosensors to visualize other cellular phenomena.
Download or read book Imaging from Cells to Animals In Vivo written by Margarida Barroso and published by CRC Press. This book was released on 2020-12-03 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers an overview of imaging techniques used to investigate cells and tissue in their native environment. It covers the range of imaging approaches used, as well as the application of those techniques to the study of biological processes in cells and whole tissues within living organisms.
Download or read book Metal Nanoclusters in Catalysis and Materials Science The Issue of Size Control written by Benedetto Corain and published by Elsevier. This book was released on 2011-08-11 with total page 471 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metal Nanoclusters in Catalysis and Materials Science: The Issue of Size Control deals with the synthesis of metal nanoclusters along all known methodologies. Physical and chemical properties of metal nanoclusters relevant to their applications in chemical processing and materials science are covered thoroughly. Special attention is given to the role of metal nanoclusters size and shape in catalytic processes and catalytic applications relevant to industrial chemical processing.An excellent text for expanding the knowledge on the chemistry and physics of metal nanoclusters. Divided in two parts; Part I deals with general aspects of the matter and Part II has to be considered a useful handbook dealing with the production of metal nanoclusters, especially from their size-control point of view.* Divided into two parts for ease of reference: general and operational * Separation of synthetic aspects, physical properties and applications* Specific attention is given to the task of metal nanoclusters size-control
Download or read book BioMEMS and Biomedical Nanotechnology written by Mihrimah Ozkan and published by Springer Science & Business Media. This book was released on 2007-04-03 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt: Contributions reporting on fundamental and applied investigations of the material science, biochemistry, and physics of biomedical microdevices with applications to Genomics and Proteomics. Topics include gene expression profiling utilizing microarray technology; imaging and sensing for gene detection and use in DNA analysis; and coverage of advanced microfluidic devices and the Humane Genome Project.
Download or read book Handbook of Biological Confocal Microscopy written by James Pawley and published by Springer Science & Business Media. This book was released on 2010-08-04 with total page 1014 pages. Available in PDF, EPUB and Kindle. Book excerpt: Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would “never have to do that again. ” That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted “the usual owe them all a great debt of gratitude. On a more personal note, I suspects” and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.
Download or read book Encyclopedia of Analytical Science written by and published by Elsevier. This book was released on 2019-04-02 with total page 5142 pages. Available in PDF, EPUB and Kindle. Book excerpt: The third edition of the Encyclopedia of Analytical Science, Ten Volume Set is a definitive collection of articles covering the latest technologies in application areas such as medicine, environmental science, food science and geology. Meticulously organized, clearly written and fully interdisciplinary, the Encyclopedia of Analytical Science, Ten Volume Set provides foundational knowledge across the scope of modern analytical chemistry, linking fundamental topics with the latest methodologies. Articles will cover three broad areas: analytical techniques (e.g., mass spectrometry, liquid chromatography, atomic spectrometry); areas of application (e.g., forensic, environmental and clinical); and analytes (e.g., arsenic, nucleic acids and polycyclic aromatic hydrocarbons), providing a one-stop resource for analytical scientists. Offers readers a one-stop resource with access to information across the entire scope of modern analytical science Presents articles split into three broad areas: analytical techniques, areas of application and and analytes, creating an ideal resource for students, researchers and professionals Provides concise and accessible information that is ideal for non-specialists and readers from undergraduate levels and higher
Download or read book Low Dimension Sensing Nanomaterials written by Mingshui Yao and published by Frontiers Media SA. This book was released on 2021-06-04 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt:
