Download or read book Evaluation of TaqMan Real time PCR for the Detection of Viable Cryptosporidium Parvum Oocysts in Environmental Water Samples written by Melissa A. Cameron and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: ABSTRACT: Cryptosporidium parvum is of growing public health concern due to its ability to survive typical water treatment processes. In order to protect the public from infection, the Environmental Protection Agency developed Method 1623 for the detection of Cryptosporidium oocysts in environmental water samples. Execution of this method is time consuming, and the results do not provide an accurate estimation of viability. Therefore, current research is focused on creating a real-time PCR method for the accurate detection of viable Cryptosporidium parvum in environmental water samples. This thesis presents the development of a real-time PCR method, and the results obtained in its use on field samples. The assay was standardized using multiple dilution series in addition to positive and negative controls. Environmental water samples were tested using this method and Method 1623 for comparison. The results were compared statistically to determine the degree of correlation between methods. The data show that the real-time PCR method correlates well to Method 1623. In addition, the assay was determined to be more cost effective and less labor intensive than Method 1623. Although these early findings are promising, additional research and development are needed before the proposed assay can be used in industry.

Download or read book Advancing Cryptosporidium Parvum Detection Methodologies written by Mark C. Jenkins and published by American Water Works Association. This book was released on 2002 with total page 90 pages. Available in PDF, EPUB and Kindle. Book excerpt: This study focused on ideal methods of detecting C. parvum oocysts which give low detection limits, specific and sensitive detection in concentraded samples, and differentiation of viable from non-viable oocysts; are usable by a water treatment facility and provide quantitative results within a few hours. Potential useful methods are continuous flow centrifugation, rel-time TaqMan PCR, cell culture inoculation followed by PCR of DNA extration, and FISH.

Download or read book Evaluation of Genotyping Techniques for Crytosporidium Parvum written by Paul A. Rochelle and published by American Water Works Association. This book was released on 2001 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt: A research teams reports on its efforts to develop and implement DNA fingerprinting techniques to characterize and differentiate the parvus species of protozoal parasite that is ubiquitous in rivers and lakes, is relatively resistant to chlorine disinfection at commonly used concentrations, and can prove fatal for immune compromised people who become infected. They evaluate fingerprinting methods based on polymerase chain reaction, determine which method is most suitable for determining strains or isolates within a species, implement the most appropriate molecular method for determining whether the isolates can be differentiated based on its animal or human host or geographical source, and determine the particular source of the protozoa contamination in source water. The report is not indexed. c. Book News Inc.

Download or read book Development of a PCR Based Method to Detect Viable Cryptosporidium Parvum Oocysts from Environmental Water Concentrates written by Debanjana Dasgupta and published by . This book was released on 2003 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Cryptosporidium Parvum Viability Assay written by Miodrag Belosevic and published by American Water Works Association. This book was released on 2000 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Cryptosporidium Parvum Oocysts written by Satomi Kato and published by . This book was released on 2001 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Quantitative Microbial Risk Assessment written by Charles N. Haas and published by John Wiley & Sons. This book was released on 2014-06-09 with total page 439 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides the latest QMRA methodologies to determine infection risk cause by either accidental microbial infections or deliberate infections caused by terrorism • Reviews the latest methodologies to quantify at every step of the microbial exposure pathways, from the first release of a pathogen to the actual human infection • Provides techniques on how to gather information, on how each microorganism moves through the environment, how to determine their survival rates on various media, and how people are exposed to the microorganism • Explains how QMRA can be used as a tool to measure the impact of interventions and identify the best policies and practices to protect public health and safety • Includes new information on genetic methods • Techniques use to develop risk models for drinking water, groundwater, recreational water, food and pathogens in the indoor environment

Download or read book Development and Standardization of a Cryptosporidium Genotyping Tool for Water Samples written by Lihua Xiao and published by American Water Works Association. This book was released on 2006 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt: CD-ROM contains data gathered in the research of this study.

Download or read book Studies on the Survival and Viability Testing of Cryptosporidium Parvum Oocysts in the Water Environment written by Marianne Heisz and published by . This book was released on 1997 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Detection of Cryptosporidium Parvum Oocysts in Soil Using the Polymerase Chain Reaction written by Juliet E. Bryant and published by . This book was released on 1995 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Detection and Quantification of Cryptosporidium Oocysts in Environmental Samples written by Megha Duggal and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Particle Association of Cryptosporidium Parvum Oocyst in Secondary Effluent written by Ryujiro Tsuchihashi and published by . This book was released on 2000 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Manual of Environmental Microbiology written by Cindy H. Nakatsu and published by John Wiley & Sons. This book was released on 2020-08-11 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The single most comprehensive resource for environmental microbiology Environmental microbiology, the study of the roles that microbes play in all planetary environments, is one of the most important areas of scientific research. The Manual of Environmental Microbiology, Fourth Edition, provides comprehensive coverage of this critical and growing field. Thoroughly updated and revised, the Manual is the definitive reference for information on microbes in air, water, and soil and their impact on human health and welfare. Written in accessible, clear prose, the manual covers four broad areas: general methodologies, environmental public health microbiology, microbial ecology, and biodegradation and biotransformation. This wealth of information is divided into 18 sections each containing chapters written by acknowledged topical experts from the international community. Specifically, this new edition of the Manual Contains completely new sections covering microbial risk assessment, quality control, and microbial source tracking Incorporates a summary of the latest methodologies used to study microorganisms in various environments Synthesizes the latest information on the assessment of microbial presence and microbial activity in natural and artificial environments The Manual of Environmental Microbiology is an essential reference for environmental microbiologists, microbial ecologists, and environmental engineers, as well as those interested in human diseases, water and wastewater treatment, and biotechnology.

Download or read book Detection and Quantitation of Cryptosporidium Parvum in Municipal Water Samples by the Polymerase Chain Reaction written by and published by . This book was released on 1997 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular based Methods for the Detection and Quantification of Infectious Cryptosporidium Parvum Oocysts in Environmental Samples written by University of Guelph. Department of Environmental Biology and published by . This book was released on 2005 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Comparison of Conventional and TaqMan Realtime RT PCR Assays for the Detection of Human Noroviruses in Environmental Water Samples written by Andrea M. Maloney and published by . This book was released on 2006 with total page 116 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book FEMS Microbiology Letters written by Federation of European Microbiological Societies and published by . This book was released on 2002 with total page 648 pages. Available in PDF, EPUB and Kindle. Book excerpt: An international journal providing for the rapid publication of short reports on microbiological research.