Download or read book Evaluation of Human Hemopoietic Stem Cell Assays for Transplantation and Gene Therapy written by Paula Boudewina van Hennik and published by . This book was released on 2001 with total page 239 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Characterization of Human Hematopoietic Stem Cells Using Gene Transfer and the Novel SCID Transplantation Assay written by André Larochelle and published by . This book was released on 1997 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The absence of preclinical repopulation assays for primitive human hematopoietic cells has hindered the development of stem cell gene therapy. Current human gene therapy trials employ gene transfer methods optimized using surrogate in vitro colony-forming cell (CFC) and longterm culture-initiating cell (LTC-IC) assays and by inference from studies on other mammalian species. In this thesis, we report the identification and characterization of a novel human hematopoietic cell, capable of repopulating the bone marrow (BM) of nonobese diabetic mice with severe combined immunodeficiency (NOD/SCID), namely the SCID-repopulating cell (SRC). Human BM or cord blood (CB) cells were infected with retroviral vectors and subsequently transplanted into NOD/SCID mice. Whereas CFC and LTC-IC were efficiently transduced with retroviruses, SRCs were transduced rarely, establishing that SRC are biologically distinct from most CFC and LTC-IC. Moreover, characterization of the cell surface phenotype indicated that SRC are exclusively CD3$\sp+$CD38$\sp-$ in contrast to CFC and LTC-IC that are mostly found in the CD34$\sp+$CD38$\sp+$ fraction after multiparameter flow sorting, confirming the distinct character of SRC. In combination with various data obtained in the past decade, these analyses provide the strongest evidence that SRC defines a novel human hematopoietic cell that is more immature than any other cell type detected to date. The inefficient infection of SRC was consistent with the low level of gene marking reported in primates and human gene therapy trials. This inability to infect the most primitive human repopulating cells, in contrast to most CFC and LTC-IC, highlights the need for appropriate pre-clinical models that will predict the outcomes of human clinical trials. As described in chapter 3 of this thesis, primitive BM cells from $\beta$-thalassemia major and sickle cell anemia (SCA) patients can engraft and proliferate in the BM of immune-deficient mice. The BM of transplanted mice contained the entire erythroid lineage from BFU-E to mature erythrocytes expressing human $\gamma$-, $\beta$- or $\rm\beta\sp{s}$-globin as in the original donor. Moreover, human erythroid cells from mice transplanted with SCA bone marrow showed characteristic sickling under reducing conditions in an in vitro assay. This system can thus be used to evaluate gene transfer efficiency into primitive human cells, longevity of expression, expression in the appropriate lineage, and correction of the disease phenotype.

Download or read book Assessment and Improvement of Gene Transfer Into Human Hematopoietic Stem Cells written by Dimitri Anthonius Breems and published by . This book was released on 1997 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Hematopoietic Stem Cell Transplantation written by Anthony D. Ho and published by CRC Press. This book was released on 2000-07-25 with total page 610 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book integrates recent advances in molecular and cell biology of hematopoietic stem cells (HSC) with developments in clinical research in stem cell-based therapy-providing an up-to-date review of novel cytokines and cellular components; animal models; cell preparation, selection, and collection; minimal residual disease and purging; expansion

Download or read book Hematopoietic Stem Cells written by Christa E. Müller-Sieburg and published by Springer. This book was released on 1992 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The idea for this volume was conceived during a discussion in the hallway at a conference in early 1990. "What is the best way to detect and define pluripotent hematopoietic stem cells?" was the question posed by Dr. Fritz Melchers. After discussing the pros and cons of the available assays for quite some time, it became apparent that this topic required a wider expertise and merited a larger forum. Thus, we decided to extend the discussion and to compile the results in this volume. Much to our delight. many of the pioneers of recent experimental and theoretical developments in stem cell research agreed to contribute their expertise to answer the question. These authors review both past findings and present insights, thus providing an overview of the evolution that has been and is occurring in the field of stem cell research. In the light of recent trailblazing developments in both experimental models and in clinical application it is indeed time to reevaluate our knowledge about stem cells. Trans plantation of hematopoietic stem cells has become more and more prevalent as a curative therapy in a variety of acquired and genetic diseases, including cancer, radiation accident, as an agent for gene therapy, and perhaps even as treatment for autoimmune diseases. Stem cells are now derived not only from bone marrow but also from peripheral blood, cord blood, and fetal liver, greatly increasing their availability for human transplantation and in some cases (fetal tissues) obliterating the need to match donors and hosts.

Download or read book Advances in Allogeneic Hematopoietic Stem Cell Transplantation written by Richard K. Burt and published by Springer. This book was released on 2012-11-28 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of hematopoietic stem cell transplantation is rapidly evolving. Realization that hematopoietic stem cells give rise to the immune compartment has resulted in clinical trials of hematopoietic stem cell transplantation for patients with autoimmune diseases. Allogeneic hematopoietic transplants are a form of adoptive immunotherapy resulting in beneficial graft versus tumor effects. Large numbers of hematopoietic cells can be collected with ease. Therefore, a renewable source of cells for ex vivo genetic manipulations is readily available. Multiple trials combining hematopoietic transplants and gene therapy are in progress. One such application is the infusion of allogeneic lymphocytes containing a suicide gene to abort graft versus host disease. Hematopoietic stem cell transplantation is in reality the clinical and practical application of cellular therapy. Hematopoietic transplant physicians are by design or by practical application evolving into cell and gene therapy specialists. The excitement and enthusiasm in hematopoietic transplantation is that it offers a door to the future. A future not of drugs or titrating poisonous chemotherapy but rather of cellular and gene therapy. 1 ALLOGENEIC PERIPHERAL BLOOD STEM CELL TRANSPLANTATION FOR HEMATOLOGIC DISEASES Martin Korbling University o/Texas MD. Anderson Cancer Center, Houston, Texas 77030 INTRODUCTION Circulating hematopoietic stem cells have emerged as an alternative to bone marrow (BM) stem cells for allografting. For many years the reconstitutive potential of circulating stem cells was questioned; peripheral blood stem cells (PBSC) were even characterized a waste product (1).

Download or read book Advances in Hematopoietic Stem Cell Transplantation and Molecular Therapy written by Rainer Haas and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 159 pages. Available in PDF, EPUB and Kindle. Book excerpt: Blood stem cell transplantation has become an established therapeutic option in the treatment of malignant diseases. At the same time molecular therapeutic approaches (i.e. gene therapy) are promising alternatives to the classical treatment of cancer. The proceedings give an overview of the biology of hematopoietic progenitor cells, peripheral stem cell transplantation in patients with hematological malignancies, recent developments in molecular diagnosis and gene therapeutic concepts, and show that successful future developments require cooperative research connecting basic science with clinical application.

Download or read book Stem Cells from Cord Blood in Utero Stem Cell Development and Transplantation Inclusive Gene Therapy written by W. Holzgreve and published by Springer. This book was released on 2001 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: The title "Stem Cells from Cord Blood, In Utero Stem Cell Develop ment, and Transplantation-Inclusive Gene Therapy" suggests that more than one topic is combined in one workshop. Indeed, at first glance the recovery of stem cells from cord blood has to be seen as separate from the attempts to achieve effective in utero therapy by stem cell trans plantation, because the first issue deals with an innovative stem cell source as an alternative to bone marrow, which is already spreading rapidly in medical practice, whereas the second topic is still strictly ex perimental and only investigated in medical centers with the appropri ate background. It is, however, not only justified, but helpful to com bine the two topics in one workshop and consequently to cover them in the same volume of the Ernst Schering Research Foundation Work shop series, because they are intimately related and both based on the new insights into the biology of stem cells. Professor Werner Arber, the Nobel Laureate from the University of Basel, pointed out in his In- Professor Dr. W. Holzgreve VI Preface The participants of the workshop troductory Lecture that our understanding of hematopoietic stem cells as descendents of totipotent cells and our current approaches to using them in post-and prenatal therapy have been furthered significantly by genetic engineering technologies which are "artificial contributions to the process of biologic evolution".

Download or read book Stem Cell Assays written by Mohan C. Vemuri and published by Springer Science & Business Media. This book was released on 2007-08-10 with total page 815 pages. Available in PDF, EPUB and Kindle. Book excerpt: The scope for improving health care using stem cell therapies is thrilling, but has considerable technical challenges and methodological constraints that need to be addressed. Keeping with the tradition of Humana Press to bring these developments to the forefront in a timely manner, this book presents scientific advances in stem cell methods for a wider use by novice and expert scientists, through the series of Methods in Molecular Biology.

Download or read book Thomas Hematopoietic Cell Transplantation 2 Volume Set written by Stephen J. Forman and published by John Wiley & Sons. This book was released on 2016-12-27 with total page 1789 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fully revised for the fifth edition, this outstanding reference on bone marrow transplantation is an essential, field-leading resource. Extensive coverage of the field, from the scientific basis for stem-cell transplantation to the future direction of research Combines the knowledge and expertise of over 170 international specialists across 106 chapters Includes new chapters addressing basic science experiments in stem-cell biology, immunology, and tolerance Contains expanded content on the benefits and challenges of transplantation, and analysis of the impact of new therapies to help clinical decision-making Includes a fully searchable Wiley Digital Edition with downloadable figures, linked references, and more References for this new edition are online only, accessible via the Wiley Digital Edition code printed inside the front cover or at www.wiley.com/go/forman/hematopoietic.

Download or read book Hematopoietic Stem Cell Therapy written by Edward David Ball and published by . This book was released on 2000 with total page 794 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book will be the only current practical guide to a widely used procedure for treating leukemias and disseminated cancers. The contents are organized chronologically, to serve as a step-by-step guide throughout the transplant process. Comprehensive yet concise, it emphasizes the latest techniques, such as peripheral blood stem cell grafts.

Download or read book Thomas Hematopoietic Cell Transplantation written by Stephen J. Forman and published by John Wiley & Sons. This book was released on 2015-12-14 with total page 1416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fully revised for the fifth edition, this outstanding reference on bone marrow transplantation is an essential, field-leading resource. Extensive coverage of the field, from the scientific basis for stem-cell transplantation to the future direction of research Combines the knowledge and expertise of over 170 international specialists across 106 chapters Includes new chapters addressing basic science experiments in stem-cell biology, immunology, and tolerance Contains expanded content on the benefits and challenges of transplantation, and analysis of the impact of new therapies to help clinical decision-making Includes a fully searchable Wiley Digital Edition with downloadable figures, linked references, and more References for this new edition are online only, accessible via the Wiley Digital Edition code printed inside the front cover or at www.wiley.com/go/forman/hematopoietic.

Download or read book Best Practices in Processing and Storage for Hematopoietic Cell Transplantation written by Joseph Schwartz and published by Springer. This book was released on 2017-12-05 with total page 163 pages. Available in PDF, EPUB and Kindle. Book excerpt: This concise book examines clinically relevant issues relating to the ways in which bone marrow, cord blood and apheresis products, are processed and stored for the purpose of Hematopoietic Cell Transplantation and Cell Therapy. The twin aims are to offer up-to-date content covering a wide spectrum of topics and controversies and to provide practitioners in the field of transplant and cell therapy with practical, immediately applicable information from the internationally experts in the field. Each chapter focuses on a particular subject, and numerous working tables, algorithms, and figures are included. Whenever appropriate, the reader’s attention is drawn to the availability of potentially high-impact clinical trials and expert practices. The authors are all experts who have been carefully selected for their knowledge of the topics that they address. The book will appeal to clinical and laboratory personnels, residents, fellows, and faculty members responsible for the care of hematopoietic cell transplant products and patients. Its format ensures that it will also serve as a robust, engaging tool to aid vital activities in every hematology and oncology trainee’s daily work.

Download or read book Quality Management and Accreditation in Hematopoietic Stem Cell Transplantation and Cellular Therapy written by Mahmoud Aljurf and published by Springer Nature. This book was released on 2021-02-19 with total page 181 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book provides a concise yet comprehensive overview on how to build a quality management program for hematopoietic stem cell transplantation (HSCT) and cellular therapy. The text reviews all the essential steps and elements necessary for establishing a quality management program and achieving accreditation in HSCT and cellular therapy. Specific areas of focus include document development and implementation, audits and validation, performance measurement, writing a quality management plan, the accreditation process, data management, and maintaining a quality management program. Written by experts in the field, Quality Management and Accreditation in Hematopoietic Stem Cell Transplantation and Cellular Therapy: A Practical Guide is a valuable resource for physicians, healthcare professionals, and laboratory staff involved in the creation and maintenance of a state-of-the-art HSCT and cellular therapy program.

Download or read book Ex Vivo Cell Therapy written by Klaus Schindhelm and published by Academic Press. This book was released on 1999 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: R.E. Nordon and K. Schindhelm, Introduction. -- L. Robb, A.G. Elefanty, and C.G. Begley, Transcriptional Control of Hematopoieses. -- R. Starr and N.A. Nicola, Cell Signaling by Hemopoietic Growth Factor Receptors. -- P.J. Simmons, D.N. Haylock, and J.-P. Lévesque, Influence of Cytokines and Adhesion Molecules on Hematopoietic Stem Cell Development. -- P.A. Rowlings, Allogeneic Hematopoietic Stem Cell Transplantation. -- U. Hahn and L.B. To, Autologous Stem Cell Transplantation. -- M.R. Vowels, Cord Blood Stem Cell Transplantation. -- S.R. Riddell, E.H. Warren, D. Lewinsohn, C. Yee, and P.D. Greenberg, Reconstitution of Immunity by Adoptive Immunotherapy with T Cells. -- L.Q. Sun, M. Miller, and G. Symonds, Exogenous Gene Transfer into Lymphoid and Hematopoietic Progenitor Cells. -- C. Dowding, T. Leemhuis, A. Jakubowski, and C. Reading, Process Development for Ex Vivo Cell Therapy. -- R.E. Nordon and K. Schindhelm, Cell Separation. -- P.W. Zandstra, C.J. Eaves, and J.M. Piret, Environ ...

Download or read book Hematopoietic Stem Cells written by Louis M. Pelus and published by Springer Nature. This book was released on 2022-10-18 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume collects updates on the technical advances in hematopoietic stem cell research and incorporates new techniques focused on the molecular/genetic, cellular, and whole organism levels. Exploring methods that apply stress to hematopoiesis, the book also contains chapters focused on better understanding the role of hematopoietic niches and their cellular components, as well as in vivo models that test and quantitate stem cell function and are key to further development of therapeutic applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Hematopoietic Stem Cells: Methods and Protocols serves as a valued addition to laboratories focused on understanding hematopoietic stem cell biology and the therapeutic advances that can be derived from it.

Download or read book Hematopoietic Stem Cell Biology written by Motonari Kondo and published by Springer Science & Business Media. This book was released on 2009-12-04 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the summer of 1988, my developmental biology professor announced to the class that hematopoietic stem cells (HSCs) had finally been purified. Somehow, I never forgot the professor’s words. When I started working in Dr. Irv Weissman’s labo- tory at Stanford as a postdoctoral fellow, I realized that the findings mentioned by the professor were from Weissman’s laboratory and had been published in a 1988 edition of the journal Science. It has been over 20 years since the publication of that seminal paper, and since then tremendous advances in understanding the biology and maturation of HSCs, namely the process of hematopoiesis, which includes lymphocyte development, have been made. These discoveries were made possible in part by advancements in technology. For example, recent availability of user friendly fluorescence activated cell sorting (FACS) machines and monoclonal an- bodies with a variety of fluorescent labels has allowed more scientists to sort and analyze rare populations in the bone marrow, such as HSCs. All classes of hematopoietic cells are derived from HSCs. Stem cell biology draws enormous attention not only from scientists, but also from ordinary people because of the tremendous potential for development of new therapeutic application to diseases that currently lack any type of effective therapy. Thus, this type of “regenerative medicine” is a relatively new and attractive field in both basic science and clinical medicine.