Download or read book Immobilization of Enzymes and Cells written by José M. Guisán and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In this way, enzymes and cells could be excellent catalysts for a much more sustainable chemical industry. However, enzymes and cells also have some limitations for nonbiological applications: fine chemistry, food chemistry, analysis, therapeutics, and so on. Enzymes and cells may be unstable, difficult to handle under nonconventional conditions, poorly selective toward synthetic substrates, and so forth. From this point of view, the transformation—from the laboratory to industry—of chemical processes catalyzed by enzymes and cells may be one of the most complex and exciting goals in biotechnology. For many industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used over very long periods of time. From this point of view, immobilization, simplicity, and stabilization have to be strongly related concepts. Over the last 30 years, a number of protocols for the immobilization of cells and enzymes have been reported in scientific literature. However, only very few protocols are simple and useful enough to greatly improve the functional properties of enzymes and cells, activity, stability, selectivity, and related properties.
Download or read book Immobilization of Cells written by Colin R. Phillips and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 171 pages. Available in PDF, EPUB and Kindle. Book excerpt: Growth of immobilized cells can be viewed as an alternative to growth of free cells in many instances. In others, immobilization confers a precision of control over the process not possible in free growth. Immobilization of cells can sometimes be considered to be a lower cost alternative to immobilization of enzymes. In this volume, immobilization procedures based on mechanical means and bonding of various types are examined, with detailed application examples. These applications include microorganisms, plant and animal cells, sub-cellular organelles and multiple enzyme systems. Particular attention is devoted to enzyme properties in immobilized cells and the properties of the carrier. The volume should provide the reader with a comprehensive overview of the subject, together with copious references. As well as serving as a research monograph, it could be used to provide reference material for a graduate course. Special thanks are due Mrs. JENNIFER KERBY for her dedicated work in the preparation of the manuscript, and IT-CHIN HSIEH for bibliographical assistance. COLIN R. PHILLIPS Toronto, July 1988 YIU C. POON v Table of Contents 1 Introduction. 1 References . 9 2 Methods of Cell Immobilization 11 2.1 Mechanical Immobilization . 11 2.1.1 Mycelial Pellet and Mat 11 2.1.2 Encapsulation .. 48 2.1.3 Dialysis Culture. . . 49 2.1.4 Entrapment. .... 50 2.2 Covalent Attachment 61 2.3 Ionic Attachment 62 2.3.1 Flocculation 62 2.3.2 Adsorption . 64 References . 66 3 Special Problems and Extended Applications . 75 3.1 Special Problems and Techniques .
Download or read book Immobilization of Enzymes and Cells written by Jose M. Guisan and published by Humana. This book was released on 2016-08-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.
Download or read book Immobilization of Enzymes and Cells written by Jose M. Guisan and published by Humana Press. This book was released on 2013-08-11 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.
Download or read book Enzymes and Immobilized Cells in Biotechnology written by Allen I. Laskin and published by Butterworth-Heinemann. This book was released on 1985 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bioreactor Immobilized Enzymes and Cells written by Murray Moo-Young and published by Springer. This book was released on 1988-01-11 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Immobilized Cells written by J. Tampion and published by Cambridge University Press. This book was released on 1987-12-10 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: This 1987 book gives a coherent overview of preparation and uses of immobilized enzymes.
Download or read book Fundamentals of Cell Immobilisation Biotechnology written by Viktor Nedovic and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 542 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Immobilisation Biotechnology Biotechnology is divided into two volumes. The first volume is dedicated to fundamental aspects of cell immobilisation while the second volume deals with the diverse applications of this technology. The first volume, Fundamentals of Cell Immobilisation Biotechnology, comprises 26 chapters arranged into four parts: Materials for cell immobilisation/encapsulation, Methods and technologies for cell immobilisation/encapsulation, Carrier characterisation and bioreactor design, and Physiology of immobilised cells: techniques and mathematical modelling.
Download or read book Immobilized Microbial Cells written by Ichiro Chibata and published by Elsevier. This book was released on 2014-06-28 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immobilized Microbial Cells, Volume 4 provides an overview of the methods of immobilization, applications, and ways of utilizing immobilized microbial cells and subcellular organelles and chloroplasts as biocatalysts. This volume is comprised of seven chapters. It begins with the historical background of immobilized cell research. Subsequent chapters focus on the methods of immobilization and applications of immobilized microbial cells, living cells, and organelles. The last two chapters discuss gas production of immobilized cells for energy generation and the chemical engineering analysis of immobilized-cell systems. The book will be of great use to chemists and chemical engineers.
Download or read book Immobilized Biosystems written by I.A. Veliky and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an overview of the immobilization of viable and non-viable cells, proteins, enzymes and active molecules, and their interaction with natural or synthetic carriers for performing biochemical and chemical reactions in vivo and in vitro.
Download or read book Industrial Application of Immobilized Biocatalysts written by Atsuo Tanaka and published by CRC Press. This book was released on 1992-10-16 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers practical examples of bioreactor systems that use immobilized biocatalysts - including enzymes and microbial cells - that have been implemented on the industrial level in Japan and Denmark. The book provides information on the current status of successful new bioreactor technologies.
Download or read book Immobilization of Enzymes and Cells written by and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Immobilized Cells Basics and Applications written by R.M. Buitelaar and published by Elsevier. This book was released on 1996-03-21 with total page 865 pages. Available in PDF, EPUB and Kindle. Book excerpt: This publication contains full papers of both oral and poster presentations of the symposium "Immobilized Cells: Basics and Applications" that was held in Noordwijkerhout, The Netherlands, 26-29 November 1995. This volume covers recent developments in the field of immobilization e.g.: new support materials, characterization of support materials, kinetic characterizations, dynamic modelling, bioreactor types, scale up and applications are also given. Applications in the field of medicine, fermentation technology, food technology and environmental technology are described. Guidelines for research with immobilized cells. Based on the scientific sessions a strategy of research and methods for characterization of immobilized cells, especially in view of applications are given. The goal was to relate basic research to applications and to extract guidelines for characterization of immobilized cells in view of process design and application from the contributions. The manuscripts presented in these proceedings give an extensive and recent overview of the research and applications of immobilized-cell technology.
Download or read book Immobilization of Enzymes and Cells written by Gordon Bickerstaff and published by Humana Press. This book was released on 1996-12-01 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immobihzatron of enzymes, cells, and organelles has expanded greatly in the past 30 years as the advantages of immobilization have been evaluated and utilized in analyttcal, biotransformation, and medical applications. A c- sequence of this explosion of technology IS that there is now a bewildering array of permutations for the immobilization of biological material. The p- pose of Immobilization of Enzymes and Cells is to provrde a basic reference tool for all academic and industrial research workers seeking to start or expand the use of mnnobilization techniques in their work. The book does not aim to provide comprehensive coverage of the vast range of methods available, but will serve as a launch pad for potential users of immobilization techniques. One reason for the vast expanse of mmrobilization technology lies m the subject material to be immobilized. Biological catalysts (enzymes, organelles, and cells) have a high degree of individual variability, and although many tmmobilization techniques have wide applicability, tt is imposstble for one or even a few methods to cater to the great diversity of requirements inherent in biological material. This is especially so when the atm is to produce an op- mum system m which the immobihzed biocatalyst will function at high levels of efficrency, stability, and so on.
Download or read book Applications of Cell Immobilisation Biotechnology written by Viktor Nedovic and published by Springer Science & Business Media. This book was released on 2006-04-06 with total page 556 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell immobilisation biotechnology is a multidisciplinary area, shown to have an important impact on many scientific subdisciplines – including biomedicine, pharmacology, cosmetology, food and agricultural sciences, beverage production, industrial waste treatment, analytical applications, biologics production. "Cell Immobilisation Biotechnology" is an outcome of the editors’ intention to collate the extensive and widespread information on fundamental aspects and applications of immobilisation/encapsulation biotechnology into a comprehensive reference work and to provide an overview of the most recent results and developments in this domain. "Cell Immobilisation Biotechnology" is divided into the two book volumes, FOBI 8A and FOBI 8B. The FOBI 8A volume, Fundamentals of Cell Immobilisation Biotechnology, is dedicated to fundamental aspects of cell immobilisation while the present volume, FOBI 8B, Applications of Cell Immobilisation Biotechnology, deals with diverse applications of this technology.
Download or read book Immobilized Enzymes written by Michael D. Trevan and published by John Wiley & Sons. This book was released on 1980 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biotechnology written by Robert D. Warmbrodt and published by . This book was released on 1991 with total page 32 pages. Available in PDF, EPUB and Kindle. Book excerpt: