Download or read book Enzyme Kinetics written by Irwin H. Segel and published by Wiley-Interscience. This book was released on 1975-05-26 with total page 994 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers enzyme kinetics from its most elementary aspects to such modern subjects as steady-state, multi-reactant kinetics and isotope exchange. Offers an understanding of the behavior of enzyme systems and the diagnostic tools used to characterize them and determine kinetic mechanisms. Illustrates and explains current subjects such as cumulative, concerted and cooperative feedback inhibition and metal ion activation.
Download or read book Enzyme Kinetics for Systems Biology written by Herbert M. Sauro and published by Future Skill Software. This book was released on 2011 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book introduces fundamental concepts in kinetics that relate to system biology. Topics include basic kinetics, enzyme kinetics, generalized rate laws, gene regulation and introduction to elasticities. The text is suitable for junior/senior undergraduates and graduates who need access to information relevant to modeling biochemical pathways.
Download or read book Enzyme Kinetics written by Alejandro G. Marangoni and published by John Wiley & Sons. This book was released on 2003-04-23 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: Practical Enzyme Kinetics provides a practical how-to guide for beginning students, technicians, and non-specialists for evaluating enzyme kinetics using common software packages to perform easy enzymatic analyses.
Download or read book Enzyme Technology written by Ashok Pandey and published by Springer Science & Business Media. This book was released on 2006-04-28 with total page 774 pages. Available in PDF, EPUB and Kindle. Book excerpt: Publisher Description
Download or read book Fundamentals of Enzyme Kinetics written by Athel Cornish-Bowden and published by Elsevier. This book was released on 2014-05-20 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fundamentals of Enzyme Kinetics details the rate of reactions catalyzed by different enzymes and the effects of varying the conditions on them. The book includes the basic principles of chemical kinetics, especially the order of a reaction and its rate constraints. The text also gives an introduction to enzyme kinetics - the idea of an enzyme-substrate complex; the Michaelis-Menten equation; the steady state treatment; and the validity of its assumption. Practical considerations, the derivation of steady-state rate equations, inhibitors and activators, and two-substrate reactions are also explained. Problems after the end of each chapter have also been added, as well as their solutions at the end of the book, to test the readers' learning. The text is highly recommended for undergraduate students in biochemistry who wish to study about enzymes or focus completely on enzymology, as most of the mathematics used in this book, which have been explained in detail to remove most barriers of understanding, is elementary.
Download or read book Biochemical Calculations written by Irwin H. Segel and published by John Wiley & Sons. This book was released on 1968 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Weak acids and based; Amino acids and peptides; Biochemical energetics; Enzyme kinetics; Spectrophotometry; Isotopes in biochemistry; Miscellaneous calculations.
Download or read book Analysis of Enzyme Kinetic Data written by Athel Cornish-Bowden and published by Oxford University Press, USA. This book was released on 1995 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book introduces the theory and practice of statistical analysis of kinetic data for enzyme-catalysed reactions in the steady state. It includes a detailed description of the use of the Leonora program for analysing enzyme kinetic data, together with the program on an IBM PC compatibledisk. Default options and a worked example provide invaluable guidance for the student and the novice and in addition Analysis of Enzyme Kinetic Data provides each reader with the necessary software and the required understanding to tailor an analysis to the requirements of their own research. Theoretical topics include basic principles of a least squares analysis; fitting the Michaelis-Menten equation by a least squares analysis; the general linear model; residual plots; maximum likdlihood and efficiency; generalised medians; and robust regression. Practical topics include examinationand fitting of statistical data; installation of Leonora, its use, simulations, MENUs, and customization.
Download or read book Catalysis in Chemistry and Enzymology written by William P. Jencks and published by Courier Corporation. This book was released on 1987-01-01 with total page 866 pages. Available in PDF, EPUB and Kindle. Book excerpt: Exceptionally clear coverage of mechanisms for catalysis, forces in aqueous solution, carbonyl- and acyl-group reactions, practical kinetics, more.
Download or read book Enzymes written by Robert A. Copeland and published by John Wiley & Sons. This book was released on 2004-04-07 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fully updated and expanded-a solid foundation for understandingexperimental enzymology. This practical, up-to-date survey is designed for a broadspectrum of biological and chemical scientists who are beginning todelve into modern enzymology. Enzymes, Second Editionexplains the structural complexities of proteins and enzymes andthe mechanisms by which enzymes perform their catalytic functions.The book provides illustrative examples from the contemporaryliterature to guide the reader through concepts and data analysisprocedures. Clear, well-written descriptions simplify the complexmathematical treatment of enzyme kinetic data, and numerouscitations at the end of each chapter enable the reader to accessthe primary literature and more in-depth treatments of specifictopics. This Second Edition of Enzymes: A Practical Introductionto Structure, Mechanism, and Data Analysis features refinedand expanded coverage of many concepts, while retaining theintroductory nature of the book. Important new featuresinclude: A new chapter on protein-ligand binding equilibria Expanded coverage of chemical mechanisms in enzyme catalysisand experimental measurements of enzyme activity Updated and refined discussions of enzyme inhibitors andmultiple substrate reactions Coverage of current practical applications to the study ofenzymology Supplemented with appendices providing contact information forsuppliers of reagents and equipment for enzyme studies, as well asa survey of useful Internet sites and computer software forenzymatic data analysis, Enzymes, Second Edition isthe ultimate practical guide for scientists and students inbiochemical, pharmaceutical, biotechnical, medicinal, andagricultural/food-related research.
Download or read book Enzyme Biocatalysis written by Andrés Illanes and published by Springer Science & Business Media. This book was released on 2008-06-19 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book was written with the purpose of providing a sound basis for the design of enzymatic reactions based on kinetic principles, but also to give an updated vision of the potentials and limitations of biocatalysis, especially with respect to recent app- cations in processes of organic synthesis. The ?rst ?ve chapters are structured in the form of a textbook, going from the basic principles of enzyme structure and fu- tion to reactor design for homogeneous systems with soluble enzymes and hete- geneous systems with immobilized enzymes. The last chapter of the book is divided into six sections that represent illustrative case studies of biocatalytic processes of industrial relevance or potential, written by experts in the respective ?elds. We sincerely hope that this book will represent an element in the toolbox of gr- uate students in applied biology and chemical and biochemical engineering and also of undergraduate students with formal training in organic chemistry, biochemistry, thermodynamics and chemical reaction kinetics. Beyond that, the book pretends also to illustrate the potential of biocatalytic processes with case studies in the ?eld of organic synthesis, which we hope will be of interest for the academia and prof- sionals involved in R&D&I. If some of our young readers are encouraged to engage or persevere in their work in biocatalysis this will certainly be our more precious reward.
Download or read book Enzyme Assays written by Robert Eisenthal and published by Practical Approach. This book was released on 2002 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used tomeasure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters.Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gelelectrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening;statistical analysis of enzyme kinetic data; and the determination of active site concentration.This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.
Download or read book Beer written by Charles Bamforth and published by Oxford University Press. This book was released on 2009-04-03 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by one of the world's leading authorities and hailed by American Brewer as "brilliant" and "by a wide margin the best reference now available," Beer offers an amusing and informative account of the art and science of brewing, examining the history of brewing and how the brewing process has evolved through the ages. The third edition features more information concerning the history of beer especially in the United States; British, Japanese, and Egyptian beer; beer in the context of health and nutrition; and the various styles of beer. Author Charles Bamforth has also added detailed sidebars on prohibition, Sierra Nevada, life as a maltster, hopgrowing in the Northwestern U.S., and how cans and bottle are made. Finally, the book includes new sections on beer in relation to food, contrasting attitudes towards beer in Europe and America, how beer is marketed, distributed, and retailed in the US, and modern ways of dealing with yeast.
Download or read book Biochemical Thermodynamics written by Robert A. Alberty and published by John Wiley & Sons. This book was released on 2006-03-31 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Navigate the complexities of biochemical thermodynamics with Mathematica(r) Chemical reactions are studied under the constraints of constant temperature and constant pressure; biochemical reactions are studied under the additional constraints of pH and, perhaps, pMg or free concentrations of other metal ions. As more intensive variables are specified, more thermodynamic properties of a system are defined, and the equations that represent thermodynamic properties as a function of independent variables become more complicated. This sequel to Robert Alberty's popular Thermodynamics of Biochemical Reactions describes how researchers will find Mathematica(r) a simple and elegant tool, which makes it possible to perform complex calculations that would previously have been impractical. Biochemical Thermodynamics: Applications of Mathematica(r) provides a comprehensive and rigorous treatment of biochemical thermodynamics using Mathematica(r) to practically resolve thermodynamic issues. Topics covered include: * Thermodynamics of the dissociation of weak acids * Apparent equilibrium constants * Biochemical reactions at specified temperatures and various pHs * Uses of matrices in biochemical thermodynamics * Oxidoreductase, transferase, hydrolase, and lyase reactions * Reactions at 298.15K * Thermodynamics of the binding of ligands by proteins * Calorimetry of biochemical reactions Because Mathematica(r) allows the intermingling of text and calculations, this book has been written in Mathematica(r) and includes a CD-ROM containing the entire book along with macros that help scientists and engineers solve their particular problems.
Download or read book Computer Modeling of Chemical Reactions in Enzymes and Solutions written by Arieh Warshel and published by Wiley-Interscience. This book was released on 1997-03-28 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical reference explores computer modeling of enzyme reations--techniques that help chemists, biochemists and pharmaceutical researchers understand drug and enzyme action.
Download or read book Industrial Enzyme Applications written by Andreas Vogel and published by John Wiley & Sons. This book was released on 2019-10-28 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference is a "must-read": It explains how an effective and economically viable enzymatic process in industry is developed and presents numerous successful examples which underline the efficiency of biocatalysis.
Download or read book Chemical Kinetics and Reaction Dynamics written by Santosh K. Upadhyay and published by Springer Science & Business Media. This book was released on 2007-04-29 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemical Kinetics and Reaction Dynamics brings together the major facts and theories relating to the rates with which chemical reactions occur from both the macroscopic and microscopic point of view. This book helps the reader achieve a thorough understanding of the principles of chemical kinetics and includes: Detailed stereochemical discussions of reaction steps Classical theory based calculations of state-to-state rate constants A collection of matters on kinetics of various special reactions such as micellar catalysis, phase transfer catalysis, inhibition processes, oscillatory reactions, solid-state reactions, and polymerization reactions at a single source. The growth of the chemical industry greatly depends on the application of chemical kinetics, catalysts and catalytic processes. This volume is therefore an invaluable resource for all academics, industrial researchers and students interested in kinetics, molecular reaction dynamics, and the mechanisms of chemical reactions.
Download or read book Evaluation of Enzyme Inhibitors in Drug Discovery written by Robert A. Copeland and published by John Wiley & Sons. This book was released on 2005-04-01 with total page 295 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vital information for discovering and optimizing new drugs "Understanding the data and the experimental details that support it has always been at the heart of good science and the assumption challenging process that leads from good science to drug discovery. This book helps medicinal chemists and pharmacologists to do exactly that in the realm of enzyme inhibitors." -Paul S. Anderson, PhD This publication provides readers with a thorough understanding of enzyme-inhibitor evaluation to assist them in their efforts to discover and optimize novel drug therapies. Key topics such as competitive, noncompetitive, and uncompetitive inhibition, slow binding, tight binding, and the use of Hill coefficients to study reaction stoichiometry are all presented. Examples of key concepts are presented with an emphasis on clinical relevance and practical applications. Targeted to medicinal chemists and pharmacologists, Evaluation of Enzyme Inhibitors in Drug Discovery focuses on the questions that they need to address: * What opportunities for inhibitor interactions with enzyme targets arise from consideration of the catalytic reaction mechanism? * How are inhibitors evaluated for potency, selectivity, and mode of action? * What are the advantages and disadvantages of specific inhibition modalities with respect to efficacy in vivo? * What information do medicinal chemists and pharmacologists need from their biochemistry and enzymology colleagues to effectively pursue lead optimization? Beginning with a discussion of the advantages of enzymes as targets for drug discovery, the publication then explores the reaction mechanisms of enzyme catalysis and the types of interactions that can occur between enzymes and inhibitory molecules that lend themselves to therapeutic use. Next are discussions of mechanistic issues that must be considered when designing enzyme assays for compound library screening and for lead optimization efforts. Finally, the publication delves into special forms of inhibition that are commonly encountered in drug discovery efforts, but can be easily overlooked or misinterpreted. This publication is designed to provide students with a solid foundation in enzymology and its role in drug discovery. Medicinal chemists and pharmacologists can refer to individual chapters as specific issues arise during the course of their ongoing drug discovery efforts.
