Download or read book Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives written by Jesús Fernández Lucas and published by John Wiley & Sons. This book was released on 2019-04-29 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: A review of innovative tools for creative nucleic acid chemists that open the door to novel probes and therapeutic agents Nucleic acids continue to gain importance as novel diagnostic and therapeutic agents. With contributions from noted scientists and scholars, Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives is a practical reference that includes a wide range of approaches for the synthesis of designer nucleic acids and their derivatives. The book covers enzymatic (including chemo-enzymatic) methods, with a focus on the synthesis and incorporation of modified nucleosides. The authors also offer a review of innovative approaches for the non-enzymatic chemical synthesis of nucleic acids and their analogs and derivatives, highlighting especially challenging species. The book offers a concise review of the methods that prepare novel and heavily modified polynucleotides in sufficient amount and purity for most clinical and research applications. This important book: -Presents a timely and topical guide to the synthesis of designer nucleic acids and their derivatives -Addresses the growing market for nucleotide-derived pharmaceuticals used as anti-infectives and chemotherapeutic agents, as well as fungicides and other agrochemicals. -Covers novel methods and the most recent trends in the field -Contains contributions from an international panel of noted scientistics Written for biochemists, medicinal chemists, natural products chemists, organic chemists, and biotechnologists, Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives is a practice-oriented guide that reviews innovative methods for the enzymatic as well as non-enzymatic synthesis of nucleic acid species.
Download or read book Modified Nucleic Acids written by Kazuhiko Nakatani and published by Springer. This book was released on 2016-04-04 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book spans diverse aspects of modified nucleic acids, from chemical synthesis and spectroscopy to in vivo applications, and highlights studies on chemical modifications of the backbone and nucleobases. Topics discussed include fluorescent pyrimidine and purine analogs, enzymatic approaches to the preparation of modified nucleic acids, emission and electron paramagnetic resonance (EPR) spectroscopy for studying nucleic acid structure and dynamics, non-covalent binding of low- and high-MW ligands to nucleic acids and the design of unnatural base pairs. This unique book addresses new developments and is designed for graduate level and professional research purposes.
Download or read book Enzymes of Nucleic Acid Synthesis and Modification written by Samson T. Jacob and published by CRC Press. This book was released on 2018-01-18 with total page 359 pages. Available in PDF, EPUB and Kindle. Book excerpt: The First volume gives an overview of the enzymes involved in DNA synthesis and modification; the second volume deals with the RNA-enzymes. Although the major emphasis of the book is on eukaryotic enzymes, a separate chapter dealing with prokaryotic DNA repair enzymes has been included to discuss the major advances in this field in recent years. There are two separate chapters on RNA polymerases to provide a comprehensive coverage of the enzymes from lower eukaryotes, plants and higher eukaryotes.
Download or read book Chemical and Enzymatic Synthesis of Gene Fragments written by Hans Günter Gassen and published by . This book was released on 1982 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: Preface: In the past the chemical and enzymatic synthesis of oligonucleotides of defined sequence had to be left to a few experts. Now, however, with the triester approach, the phosphite method and the solid-support techniques gene fragment synthesis has turned into an easy procedure even for a non-chemist. Due to the elegant chemistry involved, all methods work without sophisticated equipment and are prone to mechanisation and eventual automation. It is hoped that combined chemical-encymatic gene synthesis may become a standard technique in a molecular biology laboratory, such as DNA sequencing or in-vitro recombination of nucleic acides. We omitted chemical RNA synthesis, since this field is developing so rapidly at the moment that one has to refer to the original publications. However, we included enzymatic synthesis of RNA fragments, procedures which already have obtained a high degree of standardisation. Most of the contributions are revised versions of the protocols supplied for the EMBO sponsored course on "Automated Chemical and Enzymic Gene Synthesis", held in Darmstadt, March 21 to April 3, 1982. The protocols were improved on the basis of the experience of 30 student scientists with chemical, biological or medical backgrounds. Previously omitted procedures, such as the wandering spot method for oligonucleotide analysis, were included. In editing the manuscript we encountered problems with the nomenclature of nucleic acid components. In unambiquous cases we favoured a simple description, hoping for example, that oligodeoxynucleotide is always understood to mean oligo-2'-deoxyribonucleotide. This book aims to provide those interested in DNA/RNA research with state-of-the-art methods in the synthesis, purification, and analysis of DNA and RNA fragments. The editors wish to thank the authors for their efforts in preparing manuscripts from the the revised laboratory protocols. We gratefully acknowledge the skill and the patience of Mrs. E. Ronnfeldt in typing the manuscripts. We express our thanks to Verlag Chemie for the friendly and very efficient cooperation.--H.G. Gassen A. Lang--Darmstadt, in July 1982.
Download or read book Nucleic Acids Synthesis written by Hubert Köster and published by IRL Press. This book was released on 1980 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nucleic Acids in Chemistry and Biology written by G Michael Blackburn and published by Royal Society of Chemistry. This book was released on 2015-11-09 with total page 503 pages. Available in PDF, EPUB and Kindle. Book excerpt: The structure, function and reactions of nucleic acids are central to molecular biology and are crucial for the understanding of complex biological processes involved. Revised and updated Nucleic Acids in Chemistry and Biology 3rd Edition discusses in detail, both the chemistry and biology of nucleic acids and brings RNA into parity with DNA. Written by leading experts, with extensive teaching experience, this new edition provides some updated and expanded coverage of nucleic acid chemistry, reactions and interactions with proteins and drugs. A brief history of the discovery of nucleic acids is followed by a molecularly based introduction to the structure and biological roles of DNA and RNA. Key chapters are devoted to the chemical synthesis of nucleosides and nucleotides, oligonucleotides and their analogues and to analytical techniques applied to nucleic acids. The text is supported by an extensive list of references, making it a definitive reference source. This authoritative book presents topics in an integrated manner and readable style. It is ideal for graduate and undergraduates students of chemistry and biochemistry, as well as new researchers to the field.
Download or read book Enzymes of Nucleic Acid Synthesis and Modification written by Samson T. Jacob and published by CRC Press. This book was released on 2018-01-18 with total page 301 pages. Available in PDF, EPUB and Kindle. Book excerpt: The First volume gives an overview of the enzymes involved in DNA synthesis and modification; the second volume deals with the RNA-enzymes. Although the major emphasis of the book is on eukaryotic enzymes, a separate chapter dealing with prokaryotic DNA repair enzymes has been included to discuss the major advances in this field in recent years. There are two separate chapters on RNA polymerases to provide a comprehensive coverage of the enzymes from lower eukaryotes, plants and higher eukaryotes.
Download or read book Progress in Nucleic Acid Research and Molecular Biology written by and published by Academic Press. This book was released on 1968 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Progress in Nucleic Acid Research and Molecular Biology
Download or read book The Organic Chemistry of Nucleic Acids written by Yoshihisa Mizuno and published by Elsevier Science & Technology. This book was released on 1986 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enzymes of Nucleic Acid Synthesis and Modification written by and published by . This book was released on 1983 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Ribozymes written by Sabine Müller and published by John Wiley & Sons. This book was released on 2021-07-09 with total page 81 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ribozymes Provides comprehensive coverage of a core field in the molecular biosciences, bringing together decades of knowledge from the world’s top professionals in the field Timely and unique in its breadth of content, this all-encompassing and authoritative reference on ribozymes documents the great diversity of nucleic acid-based catalysis. It integrates the knowledge gained over the past 35 years in the field and features contributions from virtually every leading expert on the subject. Ribozymes is organized into six major parts. It starts by describing general principles and strategies of nucleic acid catalysis. It then introduces naturally occurring ribozymes and includes the search for new catalytic motifs or novel genomic locations of known motifs. Next, it covers the development and design of engineered ribozymes, before moving on to DNAzymes as a close relative of ribozymes. The next part examines the use of ribozymes for medicinal and environmental diagnostics, as well as for therapeutic tools. It finishes with a look at the tools and methods in ribozyme research, including the techniques and assays for structural and functional characterization of nucleic acid catalysts. The first reference to tie together all aspects of the multi-faceted field of ribozymes Features more than 30 comprehensive chapters in two volumes Covers the chemical principles of RNA catalysis; naturally occurring ribozymes, engineered ribozymes; DNAzymes; ribozymes as tools in diagnostics and therapy, and tools and methods to study ribozymes Includes first-hand accounts of concepts, techniques, and applications by a team of top international experts from leading academic institutions Dedicates half of its content to methods and practical applications, ranging from bioanalytical tools to medical diagnostics to therapeutics Ribozymes is an unmatched resource for all biochemists, biotechnologists, molecular biologists, and bioengineers interested in the topic.
Download or read book Synthesis of Therapeutic Oligonucleotides written by Satoshi Obika and published by Springer. This book was released on 2018-12-08 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the latest knowledge on a broad range of topics relating to the synthesis of natural and artificial oligonucleotides with therapeutic potential. Nucleic acid-based therapeutics are attracting much attention, and numerous therapeutic oligonucleotides, such as antisense oligonucleotides, siRNAs, splice-switching oligonucleotides, and nucleic acid aptamers, are being evaluated in clinical trials for the treatment of a variety of diseases. Synthesis of Therapeutic Oligonucleotides covers a broad range of topics in the field that are of high relevance to researchers, including the synthesis of natural and chemically modified oligonucleotides, the development of novel nucleic acid analogs, industrial scale synthesis and purification of oligonucleotides, and important aspects of chemistry, manufacturing, and controls (CMC). The aim is to provide new insights and inspire fresh ideas in nucleic acid chemistry that may ultimately lead to novel concepts and techniques and the discovery of more effective nucleic acid drugs. The book will be of high value for both established researchers in the field and students intending to specialize in nucleic acid chemistry research.
Download or read book DNA and RNA Modification Enzymes written by Henri Grosjean and published by CRC Press. This book was released on 2009-06-11 with total page 682 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is a timely and comprehensive description of the many facets of DNA and RNA modification-editing processes and to some extent repair mechanisms. Each chapter offers fundamental principles as well as up to date information on recent advances in the field (up to end 2008). They ended by a shortconclusion and future prospect' section and
Download or read book Enzymatic Synthesis of DNA written by Arthur Kornberg and published by . This book was released on 1961 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Current Protocols in Nucleic Acid Chemistry written by Serge L. Beaucage and published by Current Protocols. This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Good methods must be reliable, well-tested, and honed to minimize the time and expense required to achieve the desired results. CPNC provides a continuously growing and evolving set of protocols that allows researchers to benefit from the experience of other researchers around the world. The core manual provides a comprehensive set of protocols that have been compiled, revised, and streamlined over the last 6 years. Quarterly updates provide new protocols in emerging areas of research as well as continued advances and new applications for fundamental methods. The book is designed to grow and change with the field of nucleic acid chemistry. Fundamental nucleoside chemistry methods include sugar-base condensation, phosphorylation, and nucleoside protection. Methods for oligonucleotide synthesis include H-phosphonate and phosphoramidite approaches, solid-phase and solution-phase synthesis, large-scale synthesis, synthesis for modified and unmodified oligonucleotides, conjugation of oligonucleotides, synthesis without base protection, and synthesis on microarrays. More specialized synthetic methods include synthesis of biologically active nucleosides and prodrugs. Purification and characterization methods are detailed. Advanced methods include biophysical analysis, combinatorial methods, and nanotechnology. Each protocol includes rationale for choosing appropriate methods, step-by-step procedures, complete recipes, anticipated results, characterization data, and troubleshooting, as well as background and recommended reading. The level of procedural detail is far beyond that found in the research literature, and tips and comments from authors are geared towards ensuring reliable duplication in the laboratory.
Download or read book Enzymes of Nucleic Acid Synthesis and Modification written by Samson T Jacob and published by . This book was released on 1983 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nucleoside Triphosphates and their Analogs written by Morteza Vaghefi and published by CRC Press. This book was released on 2016-04-19 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: While adenosine triphosphate (ATP) is described as the universal currency of energy in all living organisms at the cellular level, the actual power lies in its phosphate tail. This book is the first dedicated to the field of nucleoside triphosphate (NTP). Its 13 chapters encompass the contributions of twenty scientists from both academia and industry. It provides collective information on the chemical, physiochemical, and biological properties of both natural and modified NTP and their application in life sciences. Three chapters review families of enzymes that depend on nucleotides for assembling DNA and RNA molecules. The appendix includes supporting NMR data.
