Download or read book Engineering Synthetic and Natural Vesicular System for Tumor targeted Drug Delivery written by Sagar Rayamajhi and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanomedicine provides exciting opportunities to solve modern-world medical problems from disease diagnosis to therapy. From the first approved nanomedicine in 1995 against cancer, Doxil®, to the recent sensation, lipid nanoparticle-based mRNA vaccine against COVID-19, nanomedicine has come a long way to the state of becoming a platform medical technology. Toward this endeavor, this dissertation is focused on lipid-based vesicles systems, synthetic liposomes, and cell-secreted extracellular vesicles (EVs), where we have explored and optimized efficient engineering techniques to achieve tumor-targeted drug delivery and diagnostic capacity. Liposomes are synthetic vesicles with an aqueous core and phospholipid bilayer and EVs are natural vesicles secreted by cells with protein-lipid bilayer and aqueous core carrying cellular information in terms of proteins and nucleic acid (mRNA, miRNA, DNA). These complementary properties when paired make them highly efficient delivery vehicles in a biological environment, an overarching goal of this dissertation. The main objective of this dissertation is to overcome two major challenges in nanoparticulate drug delivery system: 1) how to overcome endosome degradation of nanoparticles and maximize intracellular bioavailability and 2) how to overcome biological barriers for efficient delivery- rapid immune clearance, circulation stability, epithelial barriers, microenvironment barriers, cellular, and intracellular barriers. Toward this aim, we engineered a pH-sensitive liposome with pH-responsive 3[beta]-[N-(N',N'-dimethylaminoethane)-carbamoyl] (DC) moiety to avoid endosomal degradation. pH-sensitive liposome showed pH-responsive cationic properties which elevated the fusogenic characteristic of liposome at the acidic environment in the endosomes and facilitate endosomal escape via membrane fusion (Chapter 3). Although the in-vitro results were promising, the challenge to overcome the biological barrier remains. To overcome this problem, a natural messenger of the cellular system, which has been optimized with years of evolution, EVs, was used. We optimized a simple, efficient, and reproducible EVs isolation method by combining centrifugation, ultrafiltration, and size exclusion-based chromatography. Synthetic liposomes were used to engineer an EVs-based hybrid system that contributed to increasing overall yield, stability, and added functionality (Chapter 4). EVs derived from mouse macrophage J774A.1 showed preferential interaction toward cancer cells, both in vitro and in vivo mouse models showing promises for tumor-targeted drug delivery. Further, a gadolinium incorporated liposome was synthesized and hybridized with EVs to engineer a hybrid system with diagnostic capacity, which showed contrast-enhanced diagnostic characteristics as confirmed by clinical magnetic resonance imaging (Chapter 5). Finally, EVs were optimized for their reproducibility of physicochemical and functional properties. Rigorous EVs isolation technique and characterization confirmed the EVs production is significantly higher in cancer cells compared to non-cancer (Chapter 6). The nanosystems engineered in this study were successful to overcome endosomal degradation via escape, have longer retention time, good biocompatibility, efficient drug loading capacity, and tumor targeting characteristic, all of which are excellent properties for drug delivery systems. With this proof of concept and design consideration, this dissertation adds an important understanding of the vesicles-based system for tumor-targeted drug delivery. We envision that future works on the molecular mechanism behind the achieved biomimicry and tumor targeting potential can lead toward the translation of a vesicle-based system for tumor-targeted drug delivery.

Download or read book Engineering Translational Multi therapeutic Targeted Delivery Vehicles for Disease Management written by Stephen Winford Morton and published by . This book was released on 2015 with total page 191 pages. Available in PDF, EPUB and Kindle. Book excerpt: The complexity of growth, survival, and death signaling pathways in cancer continues to motivate extensive investigations using systems biology approaches to better inform treatments. Many of these drugs and drug combinations, however, are highly toxic, due to lack of targeting and poor pharmacokinetics, and are not easily delivered together due to solubility issues - requiring high levels of patient compliance over the course of treatment whereby lower, less efficacious doses of therapies are administered during multiple intravenous infusions. To address this problem, development of delivery systems that are safe/less toxic, yet capable of housing and delivering higher, more efficacious doses with sophisticated levels of control over the delivery, timing, and/or sequence of release, in order to therapeutically re-wire these signaling pathways, is essential to translate these drug combinations to the clinic. Work detailed herein addresses this challenge by developing controlled approaches for engineering and manufacturing translational nanoscale delivery systems for more efficacious, targeted treatment of cancer - specifically using layer-by-layer nanoscale assembly and liposomal manufacture. Layer-by-Layer (LbL) assembly of polyelectrolytes on solid substrates, including colloidal systems, is a well characterized, tunable approach for generating functional thin films for a variety of applications, including drug and gene delivery, tissue engineering, and bone regeneration. On the basis of electrostatics, this assembly method allows for incorporation of a broad range of materials; and due to its water-based synthesis, it allows for incorporation of a broad range of therapeutics without significant alteration of biological function. Using this approach, work described in Chapters 2-4 detail the utility of this approach. In Chapter 2, we demonstrate the ability to coat materials atop drug-loaded PLGA nanoparticle substrates that control drug release and nanoparticle pharmacokinetics in a systemic environment. In Chapter 3, we incorporate ligand-functionalized materials atop a drug-loaded liposomal core to promote tissue-level specificity for targeted treatment of cancer. In Chapter 4, we further adapt this approach towards addressing the challenge of scalably and reproducibly manufacturing LbL-functionalized nanoparticle systems by collaborating with Joseph DeSimone at UNC-Chapel Hill. In this work, we combine scalable methods, PRINT® particle fabrication, and spray-LbL deposition, to generate uniform and functional nanotechnologies that are built-to-order with precise control over composition, size, shape, and surface functionality. In Chapters 5-7, targeted liposomal technologies were synthesized to deliver drug combinations with different solubilities. Liposomes, representing the first nanomedicine systems approved and employed in the clinic, are well-characterized, simple and versatile platforms for manufacture of functional and tunable drug-carrier systems. Further, functionalization of these systems for tissue-level specificity is readily achieved by ligand conjugation of a lipid that is subsequently inserted into the dual drug-loaded liposomal vesicular membrane. Utilizing the hydrophobic and hydrophilic compartments of liposomes, we achieved high loadings of both hydrophobic and hydrophilic therapies within a singular tissue-targeted (e.g. folate) liposomal system. The resultant nature of compartmentalization inside the vesicular structure promoted the desired sequence of drug release for synergistic cancer therapy, improving tumor cell killing in vitro and significant tumor shrinkage in in vivo murine triple-negative breast cancer and non-small cell lung cancer models. In Chapters 6 and 7, we applied this approach for targeted combination therapeutic delivery for additional drug therapies (e.g. cisplatin-quisinostat (HDAC inhibitor) in a folate-targeted liposome; (+)-JQ1-temozolomide in a transferrin-targeted liposome) and disease targets (non-small cell lung cancer; brain stem gliomas). Using both LbL and liposomal manufacture, work detailed herein represents a broad skill set for engineering and manufacturing translational targeted multi-therapeutic delivery systems with high levels of control.

Download or read book Biopolymers for Biomedical and Biotechnological Applications written by Bernd H. A. Rehm and published by John Wiley & Sons. This book was released on 2020-12-01 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides insight into biopolymers, their physicochemical properties, and their biomedical and biotechnological applications This comprehensive book is a one-stop reference for the production, modifications, and assessment of biopolymers. It highlights the technical and methodological advancements in introducing biopolymers, their study, and promoted applications. "Biopolymers for Biomedical and Biotechnological Applications" begins with a general overview of biopolymers, properties, and biocompatibility. It then provides in-depth information in three dedicated sections: Biopolymers through Bioengineering and Biotechnology Venues; Polymeric Biomaterials with Wide Applications; and Biopolymers for Specific Applications. Chapters cover: advances in biocompatibility; advanced microbial polysaccharides; microbial cell factories for biomanufacturing of polysaccharides; exploitation of exopolysaccharides from lactic acid bacteria; and the new biopolymer for biomedical application called nanocellulose. Advances in mucin biopolymer research are presented, along with those in the synthesis of fibrous proteins and their applications. The book looks at microbial polyhydroxyalkanoates (PHAs), as well as natural and synthetic biopolymers in drug delivery and tissue engineering. It finishes with a chapter on the current state and applications of, and future trends in, biopolymers in regenerative medicine. * Offers a complete and thorough treatment of biopolymers from synthesis strategies and physiochemical properties to applications in industrial and medical biotechnology * Discusses the most attracted biopolymers with wide and specific applications * Takes a systematic approach to the field which allows readers to grasp and implement strategies for biomedical and biotechnological applications "Biopolymers for Biomedical and Biotechnological Applications" appeals to biotechnologists, bioengineers, and polymer chemists, as well as to those working in the biotechnological industry and institutes.

Download or read book Drug Delivery Systems Advanced Technologies Potentially Applicable in Personalised Treatment written by Jorge Coelho and published by Springer Science & Business Media. This book was released on 2013-03-15 with total page 433 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is part of a series dedicated to recent advances on preventive, predictive and personalised medicine (PPPM). It focuses on the theme of “Drug delivery systems: advanced technologies potentially applicable in personalised treatments”. The critical topics involving the development and preparation of effective drug delivery systems, such as: polymers available, self-assembly, nanotechnology, pharmaceutical formulations, three dimensional structures, molecular modeling, tailor-made solutions and technological tendencies, are carefully discussed. The understanding of these areas constitutes a paramount route to establish personalised and effective solutions for specific diseases and individuals.

Download or read book Exosomes in Cardiovascular Diseases written by Junjie Xiao and published by Springer. This book was released on 2017-09-19 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book provides and intensive overview on exosomes in cardiovascular diseases, its potential as biomarkers, as well as pathological and therapeutic effects. It firstly describes the general aspects of exosomes including the definition, formation and secretion of exosomes and highlight their roles as biomarkers and pathological and therapeutic effects in cardiovascular diseases as well. Secondly, basic aspects of exosomes including the purification methods of exosomes, exosomes content, and functional roles of the cardiovascular exosomes are summarized. Thirdly, exosomes as biomarkers of cardiovascular diseases are overviewed including their roles in diagnosis, prognosis and reaction to therapy. Fourthly, pathological effects of exosomes and therapeutic effects of exosomes are highlighted. Finally, future prospects of exosomes in cardiovascular research would be provided. This is an essential reference for researchers working in cell biology and regeneration, as well as clinicians such as cardiologist.

Download or read book Drug Delivery written by Monika Schäfer-Korting and published by Springer Science & Business Media. This book was released on 2010-03-10 with total page 506 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the view of most experts pharmacology is on drugs, targets, and actions. In the context the drug as a rule is seen as an active pharmaceutical ingredient and not as a complex mixture of chemical entities of a well defined structure. Today, we are becoming more and more aware of the fact that delivery of the active compound to the target site is a key. The present volume gives a topical overview on various modern approaches to drug targeting covering today’s options for specific carrier systems allowing successful drug treatment at various sites of the body difficult to address and allowing to increase the benefit-risk-ratio to the optimum possible.

Download or read book Smart Drug Delivery System written by Ali Demir Sezer and published by BoD – Books on Demand. This book was released on 2016-02-10 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: This contribution book collects reviews and original articles from eminent experts working in the interdisciplinary arena of novel drug delivery systems and their uses. From their direct and recent experience, the readers can achieve a wide vision on the new and ongoing potentialities of different smart drug delivery systems. Since the advent of analytical techniques and capabilities to measure particle sizes in nanometer ranges, there has been tremendous interest in the use of nanoparticles for more efficient methods of drug delivery. On the other hand, this reference discusses advances in the design, optimization, and adaptation of gene delivery systems for the treatment of cancer, cardiovascular, diabetic, genetic, and infectious diseases, and considers assessment and review procedures involved in the development of gene-based pharmaceuticals.

Download or read book Smart Nanovesicles for Drug Targeting and Delivery written by Maria Carafa and published by MDPI. This book was released on 2019-05-20 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt: We can use the short text on the SI page for the description, or you make slight modifications on it. The description/summary is only for promotion (flyer, distribution channels), and will not be included in the book You can use the short text on the SI page for the description Nanovesicles are highly-promising systems for the delivery and/or targeting of drugs, biomolecules and contrast agents. Despite the fact that initial studies in this area were performed on phospholipid vesicles, there is an ever-increasing interest in the use of other molecules to obtain smart vesicular carriers focusing on strategies for targeted delivery. These systems can be obtained using newly synthesized smart molecules, or by intelligent design of opportune carriers to achieve specific delivery to the site of action. The drug/contrast agent-containing vesicles need to be directed to precise locations within the body to obtain desired magnitude and duration of the therapeutic or diagnostic effect. This spatial control in the delivery might open new avenues to modulate drug activity while avoiding side-effects and to optimize contrast agent properties while avoiding a broad distribution in the organism. However, delivering and targeting active substances into specific tissues and cells is still a challenge in designing novel therapeutic approaches against untreatable disorders, such as tumors and degenerative diseases.

Download or read book Mesenchymal Stem Cell Derived Exosomes written by Yaoliang Tang and published by Academic Press. This book was released on 2015-09-02 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mesenchymal stem cell-derived exosomes are at the forefront of research in two of the most high profile and funded scientific areas – cardiovascular research and stem cells. Mesenchymal Stem Cell Derived Exosomes provides insight into the biofunction and molecular mechanisms, practical tools for research, and a look toward the clinical applications of this exciting phenomenon which is emerging as an effective diagnostic. Primarily focused on the cardiovascular applications where there have been the greatest advancements toward the clinic, this is the first compendium for clinical and biomedical researchers who are interested in integrating MSC-derived exosomes as a diagnostic and therapeutic tool. Introduces the MSC-exosome mediated cell-cell communication Covers the major functional benefits in current MSC-derived exosome studies Discusses strategies for the use of MSC-derived exosomes in cardiovascular therapies

Download or read book Ovarian Cancer Biomarkers written by Khalid El Bairi and published by Springer Nature. This book was released on 2021-10-09 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book comprehensively summarizes the biology, etiology, and pathology of ovarian cancer and explores the role of deep molecular and cellular profiling in the advancement of precision medicine. The initial chapter discusses our current understanding of the origin, development, progression and tumorigenesis of ovarian cancer. In turn, the book highlights the development of resistance, disease occurrence, and poor prognosis that are the hallmarks of ovarian cancer. The book then reviews the role of deep molecular and cellular profiling to overcome challenges that are associated with the treatment of ovarian cancer. It explores the use of genome-wide association analysis to identify genetic variants for the evaluation of ovarian carcinoma risk and prognostic prediction. Lastly, it highlights various diagnostic and prognostic ovarian cancer biomarkers for the development of molecular-targeted therapy.

Download or read book Nanopharmaceuticals Principles and Applications Vol 3 written by Vinod Kumar Yata and published by Springer Nature. This book was released on 2020-08-19 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the third volume on this subject and focuses on the recent advances of nanopharmaceuticals in cancer, dental, dermal and drug delivery applications and presents their safety, toxicity and therapeutic efficacy. The book also includes the transport phenomenon of nanomaterials and important pathways for drug delivery applications. It goes on to explain the toxicity of nanoparticles to different physiological systems and methods used to assess this for different organ systems using examples of in vivo systems.

Download or read book Biointerface Engineering Prospects in Medical Diagnostics and Drug Delivery written by Pranjal Chandra and published by Springer Nature. This book was released on 2020-07-21 with total page 259 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides detailed information on the surface and surface chemistry of various biointerfaces for the understanding and development of biosensors, biocompatible devices, and drug delivery systems. It highlights the role of interfacial phenomena towards the behaviour of biomolecules on different surfaces and their significance in recent applications. The book also addresses various surface engineering techniques for the modification of biomaterials that are implemented for improving biocompatibility. It provides an updated scientific concept of various interactions of biological systems with surfaces/modified surfaces at the molecular and cellular level. The chapters include various in-vitro, in-vivo, ex-vivo models to illustrate various aspects of Biointerface Engineering. Finally, the book elucidates troubleshooting strategies and future prospects of Biointerface Engineering in Medical Diagnostics and Drug Delivery.

Download or read book Brain Targeted Drug Delivery Systems written by Huile Gao and published by Academic Press. This book was released on 2018-09-20 with total page 499 pages. Available in PDF, EPUB and Kindle. Book excerpt: Brain Targeted Drug Delivery Systems: A Focus on Nanotechnology and Nanoparticulates provides a guide on nanoparticulates to both academic and industry researchers. The book discusses key points in the development of brain targeted drug delivery, summarizes available strategies, and considers the main problems and pitfalls evidenced in current studies on brain targeted drug delivery systems. As the brain is the most important organ in the human body, and disorders of the central nervous system (CNS) are the most serious threat to human life, this book highlights advances and new research in drug delivery methods to the brain. Provides an overview of brain targeting drug delivery that is useful to both academic and industry-based researchers Discusses key points in developing brain targeting drug delivery systems Summarizes and presents currently available strategies for brain targeting drug delivery Covers not only current studies and their strengths, but also gives insight into the pitfalls of current research

Download or read book Targeted Drug Delivery Concepts and Design written by Padma V. Devarajan and published by Springer. This book was released on 2014-12-08 with total page 788 pages. Available in PDF, EPUB and Kindle. Book excerpt: This authoritative volume explores the fundamental concepts and numerous applications of targeted delivery of drugs to the body. This compilation has been divided into eight sections comprised of the basic principles of drug targeting, disease and organ/organelle-based targeting, passive and active targeting strategies, and various advanced drug delivery tools such as functionalized lipidic, polymeric and inorganic nanocarriers. Together, the twenty-three chapters cover a wide range of topics in the field, including tumor and hepatic targeting, polymer-drug conjugates, nanoemulsion, physical and biophysical characteristics of nanoparticles, and in vivo imaging techniques, among others. The book also examines advanced characterization techniques, regulatory hurdles and toxicity-related issues that are key features for successful commercialization of targeted drug delivery system products. Targeted Drug Delivery is a comprehensive reference guide for drug delivery researchers, both beginners and those already working in the field.

Download or read book Engineering Polymer Systems for Improved Drug Delivery written by Rebecca A. Bader and published by John Wiley & Sons. This book was released on 2014-01-17 with total page 395 pages. Available in PDF, EPUB and Kindle. Book excerpt: Polymers have played a critical role in the rational design and application of drug delivery systems that increase the efficacy and reduce the toxicity of new and conventional therapeutics. Beginning with an introduction to the fundamentals of drug delivery, Engineering Polymer Systems for Improved Drug Delivery explores traditional drug delivery techniques as well as emerging advanced drug delivery techniques. By reviewing many types of polymeric drug delivery systems, and including key points, worked examples and homework problems, this book will serve as a guide to for specialists and non-specialists as well as a graduate level text for drug delivery courses.

Download or read book Targeted Drug Delivery written by Rachael W. Sirianni and published by Humana Press. This book was released on 2018-07-27 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume looks at the latest techniques used to enable therapeutic molecules to be targeted for site-specific delivery. The chapters in this book discuss topics such as approaches to express biologically derived and synthetic nanocarriers; strategies to assist in nanocarrier targeting to specific cells and tissues; and methods for evaluating delivery and efficiency for new classes of agents. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Targeted Drug Delivery: Methods and Protocols is a valuable resource for anyone interested in understanding the diversity of methods used in this evolving field.

Download or read book Multifunctional Nanoparticles for Drug Delivery Applications written by Sonke Svenson and published by Springer Science & Business Media. This book was released on 2012-02-22 with total page 371 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book clearly demonstrates the progression of nanoparticle therapeutics from basic research to applications. This book, unlike others covering nanoparticles used in medical applications, presents the medical challenges that can be reduced or even overcome by recent advances in nanoscale drug delivery. Each chapter highlights recent progress in the design and engineering of select multifunctional nanoparticles with topics covering targeting, imaging, delivery, diagnostics, and therapy.