Download or read book Electrospray Ion Mobility time of Flight Mass Spectrometry for the Detection of Inorganic Anions and Proteins in Aqueous Media written by Steven John Klopsch and published by . This book was released on 2007 with total page 80 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electrospray and MALDI Mass Spectrometry written by Richard B. Cole and published by John Wiley & Sons. This book was released on 2011-09-26 with total page 900 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover how advances in mass spectrometry are fueling new discoveries across a broad range of research areas Electrospray and MALDI Mass Spectrometry brings both veteran practitioners and beginning scientists up to date with the most recent trends and findings in electrospray ionization and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. In particular, this Second Edition highlights how advances in electrospray and MALDI mass spectrometry are supporting important discoveries in new and emerging fields such as proteomics and metabolomics as well as in traditional areas of chemistry and physics research. Electrospray AND MALDI Mass Spectrometry, SECOND EDITION is divided into five parts: Part A, Fundamentals of ES, explains the fundamental phenomena underlying the electrospray process, including selectivity in ionization and inherent electrochemistry, and concludes with a chapter offering a comparative inventory of source hardware Part B, Fundamentals of MALDI, confronts ionization mechanisms, instrument development, and matrix selection, and includes a final chapter that explores the special application of MALDI to obtain two-dimensional images of spatial distributions of compounds on surfaces Part C, ES and MALDI Coupling to Mass Spectrometry Instrumentation, examines the coupling of these ionization techniques to various mass analyzers, including quadrupole ion trap, time-of-flight, Fourier transform ion cyclotron resonance, and ion mobility mass spectrometers Part D, Practical Aspects of ES and MALDI, investigates analytical issues including quantification, charge-state distributions, noncovalent interactions in solution that are preserved as gas-phase ions, and various means of ion excitation in preparation for tandem mass spectrometry, and offers a guide to the interpretation of even-electron mass spectra Part E, Biological Applications of ES and MALDI, examines the role of mass spectrometry in such areas as peptide and protein characterization, carbohydrate analysis, lipid analysis, and drug discovery Written by a team of leading experts, the book not only provides a critical review of the literature, but also presents key concepts in tutorial fashion to help readers take full advantage of the latest technological breakthroughs and applications. As a result, Electrospray and MALDI Mass Spectrometry will help researchers fully leverage the power of electrospray and MALDI mass spectrometry. The judicious compartmentalization of chapters, and the pedagogic presentation style throughout, render the book highly suitable for use as a text for graduate-level courses in advanced mass spectrometry.
Download or read book Applied Electrospray Mass Spectrometry written by Birendra N. Pramanik and published by CRC Press. This book was released on 2002-02-28 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discussing strategies to determine the structure and machanisms of numerous compound classics, this book covers new chemical and elctrophoretic techniques for rapid sample preconcentration and separation. It summarizes breakthroughs in the theory and instrumentation of electrospray mass spectrometry in pharmaceutical and biomedical applications, provides practical examples for the characterization of peptides, proteins, and glycoproteins, includes applications in proteomics, combinatorial chemistry, and drug characterization. Topics include chemical and electrophoretic techniques for rapid sample preconcentration and separation, screening processes for proteins from libraries of compounds, protein folding and dynamics, and more.
Download or read book Ion Mobility Spectrometry Mass Spectrometry written by Charles L. Wilkins and published by CRC Press. This book was released on 2010-12-14 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analytical power of ion mobility spectrometry-mass spectrometry (IMS-MS) instruments is poised to advance this technology from research to analytical laboratories. Exploring these developments at this critical juncture, Ion Mobility Spectrometry-Mass Spectrometry: Theory and Applications covers the tools, techniques, and applications involved w
Download or read book Ion Mobility Mass Spectrometry written by Alison E Ashcroft and published by Royal Society of Chemistry. This book was released on 2021-11-11 with total page 621 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, the use of ion mobility separation in combination with mass spectrometry analysis has developed significantly. This technique adds a unique extra dimension enabling the in-depth analysis of a wide range of complex samples in the areas of the chemical and biological sciences. Providing a comprehensive guide to the technique, each chapter is written by an internationally recognised expert and with numerous different commercial platforms to choose from, this book will help the end users understand the practicalities of using different instruments for different ion mobility purposes. The first section provides a detailed account of the fundamentals behind the technique and the current range of available instrumentation. The second section focusses on the wide range of applications that have benefitted from ion mobility – mass spectrometry and includes topics taken from current research in the pharmaceutical, metabolomics, glycomics, and structural molecular biology fields. The book is primarily aimed at researchers, appealing to practising chemists and biochemists, as well as those in the pharmaceutical and medical fields.
Download or read book Studies of Electrospray ion Mobility Spectrometry time of flight Mass Spectrometry written by and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Time of flight Mass Spectrometry written by Robert J. Cotter and published by . This book was released on 1997 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the basic principles of time-of-flight (TOF) mass spectrometry with a strong emphasis on applications in biological research. It describes many innovative techniques, including orthogonal extraction, post source decay, and delayed extraction, and surveys ionization techniques used on TOF mass spectrometers such as electron impact, plasma desorption, SIMS, FAB, laser desorption, MALDI, and electrospray. The book features chapters on applications to peptides/proteins, oligonucleotides, and other biological macromolecules, and examines how techniques might be used in sequencing the human genome.
Download or read book Ion Mobility Spectrometry written by G.A. Eiceman and published by CRC Press. This book was released on 2013-12-10 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the turn of the twenty-first century, applications of ion mobility spectrometry (IMS) have diversified, expanding their utility in the military and security spheres and entering the realms of clinical practice and pharmaceutical exploration. Updated and expanded, the third edition of Ion Mobility Spectrometry begins with a comprehensive discu
Download or read book Electrospray Ionization Mass Spectrometry written by Richard B. Cole and published by Wiley-Interscience. This book was released on 1997-05-06 with total page 610 pages. Available in PDF, EPUB and Kindle. Book excerpt: Comprehensive, up-to-date coverage of a revolutionary technique Electrospray ionization mass spectrometry (ESI-MS) has completely changed the way physicists, chemists, and biologists view the study of large molecules. The technique derives detailed information about molecular weights and structures from extremely small sample quantities. ESI-MS can create highly charged forms of very high molecular weight compounds, it is naturally compatible with many types of separation techniques, and it allows noncovalent interactions between molecules in solution to be preserved in the gas phase. But many researchers may not use the technique to its full potential because they are unfamiliar with the different perspectives of its underlying processes, the varied approaches to implementation, and the wide-ranging utility of the technique. In this book, Richard B. Cole and an assemblage of leading researchers present a single-volume compilation of different approaches to the understanding and exploitation of ESI-MS. This comprehensive guide: * Examines the physical and chemical aspects of the electrospray process and describes the events involved in ion formation as well as the electro-chemical phenomena that are central to charged droplet formation during the process * Explores the coupling of electrospray ionization to various mass spectrometers, including quadrupole, magnetic, time-of-flight, quadrupole ion trap, and Fourier transform ion cyclotron resonance instruments * Describes recent progress in interfacing ESI with solution-based separation techniques, including liquid chromatography and capillary electrophoresis * Charts the rapid development of ESI applications and categorizes them by compound type: peptides and proteins, nucleic acids and their constituents, carbohydrates and lipids, small molecules related to pharmacology and drug metabolism, and organometallics and inorganic compounds Electrospray Ionization Mass Spectrometry is the indispensable handbook and reference for anyone who wishes to understand, implement, or apply this technique, including researchers in chemistry, metallochemistry, biochemistry, biology, pharmacology, and physics.
Download or read book Multidimensional Ion Mobility Spectrometry Coupled to Time of flight Mass Spectrometry written by Stormy Lee Ann Koeniger and published by . This book was released on 2006 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: For characterization of proteomic mixtures, high-quality primary sequence information is necessary to positively identify the components with database search algorithms. To improve parallel sequencing capabilities with this technology, a new drift tube design has been developed. This design provides high-efficiency fragmentation and improves the transmission efficiency of the fragments to the time-of-flight mass spectrometer. Additionally, the new method of collisional activation utilizes a system of potential at the exit of the drift tube, allowing rapid modulation of the potentials to provide precursor and fragment ion information during the course of a single LC separation.
Download or read book Development of Paired Ion Electrospray Ionization Mass Spectrometry for Ultrasensitive Detection of Anions written by Chengdong Xu and published by . This book was released on 2014 with total page 163 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry (MS) has emerged as an essential analytical technique over the last several decades. It is a widely applicable and powerful tool for determination and identification of different types of molecules. Electrospray ionization (ESI) is one of the most commonly used ionization sources for mass spectrometry. ESI-MS has advantages for the analysis of both small molecules and macromolecules due to its outstanding sensitivity, specificity, speed, and the capability for analyte structure elucidation. Currently, one challenging aspect in ESI-MS is the qualitative and quantitative determination of anionic species. This is due to the inherently poor detection sensitivity in the negative ion mode ESI-MS resulting from the prevalent electrical discharge (corona discharge). This dissertation discusses the development of a novel technique named paired ion electrospray ionization mass spectrometry (PIESI-MS) for the ultrasensitive determination of anions in the positive ion mode ESI-MS. PIESI-MS involves introducing low concentrations of structurally optimized ion-pairing reagents (IPRs) into the sample flow, thereby allowing the anions to be measured with extremely high sensitivity in the positive ion mode as the anion/IPR associated complexes. The reported LODs by PIESI-MS were usually from 2 to 3 orders of magnitude better than these of other known methods. Specifically, this dissertation describes research in two areas: 1). Novel methodologies, based on HPLC-PIESI-MS, were developed and evaluated for the trace analysis of anionic molecules. The PIESI-MS approach was found to be suitable for ultrasensitive determination of a variety of molecules, including small inorganic anions, medium sized organic anions, zwitterionic molecules, and metal cations. The absolute detection limits (LODs) of these analytes obtained using the PIESI-MS approach were determined in both single ion monitoring mode (SIM) and selected reaction monitoring mode (SRM). LODs from sub-picogram to picogram were usually observed for most of the anions, which indicates 2 to 3 orders of magnitude improvement compared to most other reported methods. The accuracy, precision, linearity, and specificity were also evaluated as a part of method validation. SRM fragmentation pathways of the analyte/IPR complexes during the collision induced dissociation were examined. 2). Novel unsymmetrical dicationic ion-pairing reagents were rationally synthesized and their performance on detection of anions by PIESI-MS were investigated. It was found that the unsymmetrical dications provided more sensitive detection by 1.5 to 12 times than their symmetrical dication analogues. The effective concentration range of the unsymmetrical dicationic ion-pairing reagents was optimized. A correlation was observed between the ESI response and the surface activity of the anion/IPR complex, which indicates that the analyte surface activity plays a vital role in the electrospray ionization process. The sensitivity enhancement mechanism in PIESI-MS was further explored based on the concepts of the equilibrium partitioning model.
Download or read book The Development and Characterization of Ion Detectors and Levitated Ion Sources for Time of flight Mass Spectrometry written by and published by . This book was released on 2015 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry (MS) plays an important role in the analysis of complex protein samples, which is performed primarily with two strategies: the bottom-up and top-down approaches. The latter has the advantage of preserving the crucial protein-level information by directly analyzing the intact proteins. Time-of-flight (TOF) mass spectrometry is heavily employed in intact protein detection due to its compatibility with the high m/z range of singly charged proteins produced by matrix-assisted laser desorption/ionization (MALDI). However, a significant challenge in TOF-MS is the limited efficiency of detecting large and slow-moving protein ions. Part of the reason lies in the TOF detectors. Conventional detectors, i.e. electron multipliers and microchannel plate (MCP) detectors, are based upon the generation of secondary electrons at ion collision, which is well known to have a decreasing efficiency as the ion velocity decreases. This relationship between ion mass and ion detection efficiency is characterized in detail. The response of an MCP detector as a function of ion mass and acceleration voltage was characterized, for singly charged peptide/protein ions ranging from 1 to 290 kDa in mass, and for acceleration voltages from 5 to 25 kV. New types of detectors are under development to overcome the detection limit for large molecules. We are developing a novel nanomembrane detector, which contains a freestanding nanomembrane that responds to ion collisions with field emission current changes. The detection efficiencies and mechanisms of different nanomembranes for 9 peptide/protein samples at various acceleration voltages were explored in a MALDI-TOF instrument. Atmospheric aerosols have serious influences on our climate and health. Understanding of their formation is important but difficult with current research tools. We are developing a novel experimental approach that combines optical tweezers for droplet levitation with control of gas-phase composition and droplet analysis using TOF mass spectrometry. MS analysis of a levitated droplet in a Bessel beam optical tweezers was performed using electrospray ionization and mass spectra of the analyte in the droplet were obtained.
Download or read book Mass Spectrometry written by Jürgen H Gross and published by Springer Science & Business Media. This book was released on 2006-04-05 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass Spectrometry is an ideal textbook for students and professionals as well as newcomers to the field. Starting from the very first principles of gas-phase ion chemistry and isotopic properties, the textbook takes the reader through the design of mass analyzers and ionization methods all the way to mass spectral interpretation and coupling techniques. Step-by-step, the reader learns how mass spectrometry works and what it can do. The book comprises a balanced mixture of practice-oriented information and theoretical background. It features a clear layout and a wealth of high-quality figures. Exercises and solutions are located on the Springer Global Web.
Download or read book Differential Ion Mobility Spectrometry written by Alexandre A. Shvartsburg and published by CRC Press. This book was released on 2008-12-24 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, scientific and engineering interests have been shifting from conventional ion mobility spectrometry (IMS) to field asymmetric waveform ion mobility spectrometry (FAIMS). Differential Ion Mobility Spectrometry: Nonlinear Ion Transport and Fundamentals of FAIMS explores this new analytical technology that separates and characterizes ions by the difference between their mobility in gases at high and low electric fields. It also covers the novel topics of higher-order differential IMS and IMS with alignment of dipole direction. The book relates the fundamentals of FAIMS and other nonlinear IMS methods to the physics of gas-phase ion transport. It begins with the basics of ion diffusion and mobility in gases, covering the main attributes of conventional IMS that are relevant to all IMS approaches. Building on this foundation, the author reviews diverse high-field transport phenomena that underlie differential IMS. He discusses the conceptual implementation and first-principles optimization of FAIMS as a filtering technique, emphasizing the dependence of FAIMS performance metrics on instrumental parameters and properties of ion species. He also explores ion reactions in FAIMS caused by field heating and the effects of inhomogeneous electric field in curved FAIMS gaps. Written by an accomplished scientist in the field, this state-of-the-art book supplies the foundation to understand the new technology of nonlinear IMS methods.
Download or read book Applications of Ion Exchange Materials in Biomedical Industries written by Inamuddin and published by Springer. This book was released on 2019-01-30 with total page 225 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the applications of ion-exchange materials in the biomedical industries. It includes topics related to the application of ion exchange chromatography in determination, extraction and separation of various compounds such as amino acids, morphine, antibiotics, nucleotides, penicillin and many more. This title is a highly valuable source of knowledge on ion-exchange materials and their applications suitable for postgraduate students and researchers but also to industrial R&D specialists in chemistry, chemical, and biochemical technology. Additionally, this book will provide an in-depth knowledge of ion-exchange column and operations suitable for engineers and industrialists.
Download or read book Rays of Positive Electricity and Their Application to Chemical Analyses written by Joseph John Thomson and published by . This book was released on 1913 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Index Medicus written by and published by . This book was released on 2003 with total page 1972 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
