Download or read book Principles and Techniques of Biochemistry and Molecular Biology written by Keith Wilson and published by Cambridge University Press. This book was released on 2010-03-04 with total page 769 pages. Available in PDF, EPUB and Kindle. Book excerpt: Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Download or read book Electrophoretic and Isoelectric Focusing Techniques in Fisheries Management written by Donald H. Whitmore and published by CRC Press. This book was released on 1990-09-24 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt: Probably the most ubiquitous biochemical method used today for examining the genetics of individuals, populations, or phylogenetic relationships between taxa is electrophoresis. This book has been created to offer a viewpoint regarding current electrophoretic separation methodologies of macromolecules and their major applications to fisheries management. The chapters in this book have been selected and organized into three sections to create a carefully blended mixture of methodologies and applications designed to educate the novice, as well as stimulate interest in professional researchers currently using electrophoresis for their work. The first section includes chapters that discuss the principles that explain the genetic basis of multiple molecular forms of proteins, the theory and practice of DNA analyses, and the methodology of electrophoretic separation of these macromolecules; starch gel electrophoresis as the predominant electrophoretic tool for fisheries genetics; and protein isoelectric focusing and DNA analysis. The second section describes a variety of applications for electrophoretic techniques. The third section presents a discussion and results of experiments conducted by Dennis Powers and his associates regarding the physiological significance of multiple forms of enzymes using the fish Fundulus heteroclitus as a model system. The book features a catalog of nearly 100 enzyme staining recipes and covers new areas in electrophoretic work, such as DNA fingerprinting, genetic tags, mitochondrial DNA methodologies, and genomic manipulation of fish stocks. This book will provide a useful reference resource for fisheries biologists at federal, state, and local levels; fisheries researchers at universities; and students pursuing degrees involving research in fish genetics.
Download or read book Electrophoresis in Practice written by Reiner Westermeier and published by John Wiley & Sons. This book was released on 2016-05-16 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Electrophoresis in Practice" ist seit mehr als zwei Jahrzehnten das Standardwerk in der Elektrophorese. Die 5. Auflage wurde sorgfältig überarbeitet und beinhaltet nun ein erweitertes Kapitel zu Mikromethoden und der chipgebundenen Elektrophorese.
Download or read book Zone Electrophoresis written by Ivor Smith and published by Elsevier. This book was released on 2013-09-03 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromatography and Electrophoretic Techniques, Volume II: Zone Electrophoresis presents a number of methods, all based on zone electrophoresis, which has been carried out on commercially available apparatus offering many advantages to the majority of laboratories. This book is composed of six chapters and begins with discussions on the principles, instrumentation, and applications of paper electrophoresis at low voltages, such as voltages not exceeding 300-400 volts or a potential drop of not more than 10 volts/cm in the direction of migration. The next chapter describes the general experimental methods for the separation of abnormal hemoglobins and surveys the application of paper electrophoresis to the final identification of a hemoglobin variant. The remaining chapters deal with the principles, apparatus, reagents, and applications of other zone electrophoretic techniques, including cellulose acetate; agar gel, starch block, starch gel, and sponge rubber; high voltage paper; and continuous electrophoresis. This book will prove useful to analytical chemists and biologists.
Download or read book Chromatographic and Electrophoretic Techniques written by Ivor Smith and published by . This book was released on 1976 with total page 548 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Two Dimensional Electrophoresis and Immunological Techniques written by Bonnie S. Dunbar and published by Springer. This book was released on 1988-07-31 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text is a summary of basic principles and techniques and is dedicated to all those students who have been told by their mentors, "Go forth and do two-dimensional gels and have the results on my desk tomorrow. " No attempt has been made in this text to provide exhaustive lists of references related to basic principles or techniques or to list every com pany or supplier involved in this area of research. Nevertheless, it is hoped that sufficient information is given to help a new investigator or student appreciate the complexities but develop sufficient expertise to carry out these techniques successfully. The discussions are designed to instill in basic science and clinical investigators of all levels of expertise an appreciation of the power of combining a variety of techniques as well as to provide basic insight into the theories, complexities, and problems frequently encountered with electrophoretic and immunochemical meth ods. Bonnie S. Dunbar Houston v Acknowledgments I wish to thank my students and staff for their patience and support throughout the preparation of this text. I would like to acknowledge my appreciation for my extensive discussions with Dr. David Sammons (Uni versity of Arizona) and to Dr. N. L. and Dr. N. G. Anderson and their colleagues (Argonne National Laboratory) for their invaluable advice and suggestions in this area over the years. I thank my research assistant, Ms.
Download or read book Analysis of Peptides and Proteins by Electrophoretic Techniques written by Angela R. Piergiovanni and published by MDPI. This book was released on 2019-07-23 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt: The characterization of peptides and proteins is central to understanding their function and expression in biological matrices. Moreover, these macromolecules are important biomarkers of many human diseases. In recent years, the performance of separation techniques based on electromigration have significantly increased. The development of microdevices has reduced sample consumption and waste production while high-sensitivity detectors, such as mass spectrometry (MS) or laser-induced fluorescence (LIF), have significantly improved with regards to separation efficiency and detection limits. All of these advancements have led to appreciably enlarged fields of application. Nowadays, a multitude of studies using separation techniques based on electromigration to study proteins and peptides from numerous real matrices are available in the literature. This Special Issue covers the most recent knowledge and advances in the study of peptides and proteins using several electrophoresis techniques, as well as the characterization of relevant proteins and peptides in application areas such as clinical studies, functional foods, and toxicology.
Download or read book Electrophoretic Techniques written by Colin F. Simpson and published by . This book was released on 1983 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electrophoresis of Enzymes written by Gunter M. Rothe and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt: The electrophoresis of enzymes and isoenzymes is a well established technique in biochemical, clinical, environmental, microbiological, botanical and forensic laboratories and classical electrophoresis is presently undergoing a remarkable revival. This book compiles facts and methods on enzyme electrophoresis widely dispersed in hundreds of publications. The author summarizes them in clearly readable tables, in many carefully worked out electrophoresis and more than 140 staining protocols. The exhaustive practical experience of the author and the wealth of material summarized and reviewed makes this book a "must" for every enzyme laboratory. It will supply the practitioner with profound information on state-of-the-art enzyme electrophoresis.
Download or read book Disc Electrophoresis and Related Techniques of Polyacrylamide Gel Electrophoresis written by H. R. Maurer and published by Walter de Gruyter. This book was released on 2011-06-15 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electrophoresis in Practice written by Reiner Westermeier and published by John Wiley & Sons. This book was released on 2016-03-16 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fifth edition of the successful, long-selling classic has been completely revised and expanded, omitting some topics on obsolete DNA electrophoresis, but now with a completely new section on electrophoretic micro-methods and on-the-chip electrophoresis. The text is geared towards advanced students and professionals and contains extended background sections, protocols and a trouble-shooting section. It is now also backed by a supplementary website providing all the figures for teaching purposes, as well as a selection of animated figures tested in many workshops to explain the underlying principles of the different electrophoretic methods.
Download or read book New Directions in Electrophoretic Methods written by James W. Jorgenson and published by . This book was released on 1987 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: Annotation Developed from a symposium at the 190th Meeting of the American Chemical Society, Chicago, Illinois, September 8-13, 1985. Electrophoresis remains the most powerful method available for separating and analyzing complex mixtures of charged biopolymers, playing a key role in the latest advances in medicine, agriculture, chemistry, and biotechnology. This sixteen-chapter volume traces recent developments in electrophoretic separations, technology, and applications. Isotachophoresis, zone electrophoresis, isoelectric focusing, two dimensional electrophoresis, and pulsed electrophoresis are among the methods discussed. Additional topics include applications of electrophoretic methods in agriculture and forensic serology, the development of standards for electrophoresis, and synthetic ion-containing polymers. Annotation(c) 2003 Book News, Inc., Portland, OR (booknews.com).
Download or read book The Dynamics of Electrophoresis written by Richard A. Mosher and published by . This book was released on 1992 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a theory of electrophoresis, based on a unique combination of computer simulation and experimental data. For chromotagraphers, separation scientists, and chemical engineers, describes a unified mathematical model that handles weak and strong acids and bases, ampholytes, and proteins; and covers the four electrophoretic techniques: isoelectric focusing, isotachophoresis, and zone and moving-boundary electrophoreses. Also useful in demonstrating a new approach to constructing a model. Annotation copyright by Book News, Inc., Portland, OR
Download or read book Electrophoresis in the Separation of Biological Macromolecules written by Ö Gaal and published by John Wiley & Sons. This book was released on 1980 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: Theorical and technical bases of electrophoretic methods; Principles of electrophoresis; Moving boundary electrophoresis; Eletrophoresis of proteins; Electrophoresis bahavior of proteins; Separation of proteins according to their molecular size: estimation of molecular weights; Two-dimensional electrophoretic techniques; Staining of proteins; Detection of proteins; Electrophoretic separation of certain groups of proteins; Electrophoresis of nucleic acids and nucleoproteins; Estimation of the molecular weight of polynucleotides; Electrophoretic separtion of glycosaminoglycans.
Download or read book Practical Capillary Electrophoresis written by Robert Weinberger and published by Academic Press. This book was released on 2000-02-16 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary zone electrophoresis : basic concepts -- Capillary zone electrophoresis : methods development -- Capillary zone electrophoresis : secondary equilibrium, micelles, cyclodextrins, and related reagents -- Capillary isoelectric focusing -- Size separations in capillary gels and polymer networks -- Capillary electrochromatography -- Injection -- Detection -- Putting it all together.
Download or read book Gel Electrophoresis and Isoelectric Focusing of Proteins written by Robert C. Allen and published by Walter de Gruyter GmbH & Co KG. This book was released on 2019-05-20 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: No detailed description available for "Gel Electrophoresis and Isoelectric Focusing of Proteins".
Download or read book Chromatographic and Electrophoretic Techniques written by Ivor Smith and published by . This book was released on 1968 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt:
