Download or read book Electron Probe X ray Microanalysis of Medical and Biological Specimens written by A. J. Tousimis and published by . This book was released on 1964 with total page 15 pages. Available in PDF, EPUB and Kindle. Book excerpt: The need for local microchemical analysis at the micron level and a summarized review of the literature on the electron probe application in biology and medicine are presented. The author's procedures for preparation of biological specimens, electron probe effects on the specimen, and results from microanalysis studies on the following topics are given: (1) calcified corpuscles used as standard test specimens, (2) normal growth of the mammalian long bone, (3) hardening of blood vessels, (4) degeneration of cancer cells, and (5) Wilson's disease. Qualitative data are presented in all cases, and quantitative data for calcium and phosphorus in both normal and pathologic calcification processes. Light microscopy, electron microscopy, X-ray contact microradiography, and X-ray diffraction were carried out simultaneously. Some of these results are correlated with those obtained by the electron probe microanalysis method.
Download or read book Electron Probe Microanalysis written by Karl Zierold and published by Springer Science & Business Media. This book was released on 2013-03-08 with total page 331 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of electron probe microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure and composition. In particular, energy spectroscopy of X-rays and electrons after interaction of the electron beam with the specimen is used for this purpose. However, the application of this method in biology and medicine has to overcome three specific problems: 1. The principle constituent of most cell samples is water. Since liquid water is not compatible with vacuum conditions in the electron microscope, specimens have to be prepared without disturbing the other components, in parti cular diffusible ions (elements). 2. Electron probe microanaly sis provides physical data on either dry specimens or fully hydrated, frozen specimens. This data usually has to be con verted into quantitative data meaningful to the cell biologist or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo gical processes requires sampling techniques which are adapted to address specific biological or medical questions. During recent years, remarkable progress has been made to overcome these problems. Cryopreparation, image analysis, and electron energy loss spectroscopy are key areas which have solved some problems and offer promise for future improvements.
Download or read book X ray Microanalysis for Biologists written by Alice Warley and published by Ashgate Publishing. This book was released on 1997 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: The smart way to learn how to build InfoPath forms for SharePoint - one step at a time. Design and build forms without writing code, add approval workflows to your forms, integrate data, create and use forms in the cloud.
Download or read book X ray Microanalysis in Biology written by David C. Sigee and published by Cambridge University Press. This book was released on 1993-04-22 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes an integrated approach to the use of X-ray microanalysis in biology.
Download or read book X ray Microanalysis in Electron Microscopy for Biologists written by A. John Morgan and published by Oxford University Press, USA. This book was released on 1985 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt: This compact guide provides a straightforward introduction to electron microprobe x-ray analysis, a nondestructive technique that greatly facilitates the study of the chemistry of cells. Assuming no prior knowledge of electron optics, Morgan explains the principle of x-ray production and detection, describes the various methods for converting measured x-ray intensities to element concentrations in thin specimens, and directs the reader to primary sources for more definitive practical guidelines. A painless introduction to a powerful laboratory technique, this book will be a useful aid for cell biologists, biological electron microscopists, and electrolyte physiologists.
Download or read book Basic Methods in Biological X ray Microanalysis written by Godfried M. Roomans and published by . This book was released on 1983 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book X ray Microanalysis in Biology written by M. A. Hayat and published by . This book was released on 1980 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Scanning Electron Microscopy and X Ray Microanalysis written by Joseph Goldstein and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book has evolved by processes of selection and expansion from its predecessor, Practical Scanning Electron Microscopy (PSEM), published by Plenum Press in 1975. The interaction of the authors with students at the Short Course on Scanning Electron Microscopy and X-Ray Microanalysis held annually at Lehigh University has helped greatly in developing this textbook. The material has been chosen to provide a student with a general introduction to the techniques of scanning electron microscopy and x-ray microanalysis suitable for application in such fields as biology, geology, solid state physics, and materials science. Following the format of PSEM, this book gives the student a basic knowledge of (1) the user-controlled functions of the electron optics of the scanning electron microscope and electron microprobe, (2) the characteristics of electron-beam-sample inter actions, (3) image formation and interpretation, (4) x-ray spectrometry, and (5) quantitative x-ray microanalysis. Each of these topics has been updated and in most cases expanded over the material presented in PSEM in order to give the reader sufficient coverage to understand these topics and apply the information in the laboratory. Throughout the text, we have attempted to emphasize practical aspects of the techniques, describing those instru ment parameters which the microscopist can and must manipulate to obtain optimum information from the specimen. Certain areas in particular have been expanded in response to their increasing importance in the SEM field. Thus energy-dispersive x-ray spectrometry, which has undergone a tremendous surge in growth, is treated in substantial detail.
Download or read book Quantitative Electron Probe X ray Microanalysis of Biological Specimens written by Godfried M. Roomans and published by . This book was released on 1980 with total page 30 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book X ray and Electron Probe Analysis in Biomedical Research written by Kenneth Martin Earle and published by . This book was released on 1969 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Sample Preparation for Scanning Electron Microscopy and X Ray Microanalysis written by Patrick Echlin and published by Springer Science & Business Media. This book was released on 2011-04-14 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scanning electr on microscopy (SEM) and x-ray microanalysis can produce magnified images and in situ chemical information from virtually any type of specimen. The two instruments generally operate in a high vacuum and a very dry environment in order to produce the high energy beam of electrons needed for imaging and analysis. With a few notable exceptions, most specimens destined for study in the SEM are poor conductors and composed of beam sensitive light elements containing variable amounts of water. In the SEM, the imaging system depends on the specimen being sufficiently electrically conductive to ensure that the bulk of the incoming electrons go to ground. The formation of the image depends on collecting the different signals that are scattered as a consequence of the high energy beam interacting with the sample. Backscattered electrons and secondary electrons are generated within the primary beam-sample interactive volume and are the two principal signals used to form images. The backscattered electron coefficient ( ? ) increases with increasing atomic number of the specimen, whereas the secondary electron coefficient ( ? ) is relatively insensitive to atomic number. This fundamental diff- ence in the two signals can have an important effect on the way samples may need to be prepared. The analytical system depends on collecting the x-ray photons that are generated within the sample as a consequence of interaction with the same high energy beam of primary electrons used to produce images.
Download or read book Biomedical Applications of Microprobe Analysis written by Peter Ingram and published by Academic Press. This book was released on 1999-10-29 with total page 573 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical Applications of Microprobe Analysis is a combination reference/laboratory manual for the use of microprobe analysis in both clinical diagnostic and research settings. Also called microchemical microscopy, microprobe analysis uses high-energy bombardment of cells and tissue, in combination with high resolution EM or confocal microscopy to provide a profile of the ion, metal, and mineral concentrations present in a sample. This allows insight into the physiology and pathophysiology of a wide variety of cells and tissues.This book describes methods for obtaining detailed information about the identity and composition of particles too small to be seen with the naked eye and describes how this information can be useful in diagnostic and biomedical research. Up-to-date review of electron microprobe analysis Detailed descriptions of sample preparation techniques Recent technologies including confocal microscopy, infrared microspectroscopy, and laser raman spectroscopy Over 100 illustrations with numerous specific applications Contributions by world-renowned experts in the field Brief summary of highlights precedes each chapter
Download or read book Electron Probe Microanalysis in Biology written by David A. Erasmus and published by . This book was released on 1978 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electron Microscopy in Human Medicine written by Jan Vincents Johannessen and published by McGraw-Hill Companies. This book was released on 1983 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Scanning Electron Microscopy and X Ray Microanalysis written by Joseph Goldstein and published by Springer. This book was released on 2013-03-20 with total page 673 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book has evolved by processes of selection and expansion from its predecessor, Practical Scanning Electron Microscopy (PSEM), published by Plenum Press in 1975. The interaction of the authors with students at the Short Course on Scanning Electron Microscopy and X-Ray Microanalysis held annually at Lehigh University has helped greatly in developing this textbook. The material has been chosen to provide a student with a general introduction to the techniques of scanning electron microscopy and x-ray microanalysis suitable for application in such fields as biology, geology, solid state physics, and materials science. Following the format of PSEM, this book gives the student a basic knowledge of (1) the user-controlled functions of the electron optics of the scanning electron microscope and electron microprobe, (2) the characteristics of electron-beam-sample inter actions, (3) image formation and interpretation, (4) x-ray spectrometry, and (5) quantitative x-ray microanalysis. Each of these topics has been updated and in most cases expanded over the material presented in PSEM in order to give the reader sufficient coverage to understand these topics and apply the information in the laboratory. Throughout the text, we have attempted to emphasize practical aspects of the techniques, describing those instru ment parameters which the microscopist can and must manipulate to obtain optimum information from the specimen. Certain areas in particular have been expanded in response to their increasing importance in the SEM field. Thus energy-dispersive x-ray spectrometry, which has undergone a tremendous surge in growth, is treated in substantial detail.
Download or read book Microprobe Analysis in Medicine written by Peter Ingram and published by . This book was released on 1989 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: A work reviewing the applications of new microprobe analysis techniques in medicine. Authoritative chapters illustrate a variety of techniques and demonstrate appropriate applications to specific diseases. Key coverage includes pulmonary pathology and asbestoses.
Download or read book X ray Microanalysis for Biologists written by Alice Warley and published by Ashgate Publishing. This book was released on 1997 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: The smart way to learn how to build InfoPath forms for SharePoint - one step at a time. Design and build forms without writing code, add approval workflows to your forms, integrate data, create and use forms in the cloud.
