Download or read book Electrokinetic Transport Trapping and Sensing in Integrated Micro and Nanofluidic Devices written by Michelle Lynn Kovarik and published by . This book was released on 2009 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Electrokinetic Transport Phenomena in Nanochannels and Applications of Nanochannel based Devices in Nanoparticle Detection and Molecule Sensing written by Ran Peng and published by . This book was released on 2018 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanofluidics investigates transport phenomena, manipulation, and control of fluids and nano-sized objects in fluidic channels with an at least one-dimensional size smaller than 100 nm. At the nanoscale, fluids and objects exhibit lots of unique physical and mechanical behaviors which cannot be observed in micron-sized or macro-sized structures, especially for electrokinetically driven cases. Electrokinetic transport phenomena in nanochannels offer promising possibilities for new applications of nanofluidic systems, such as drug delivery, DNA sequencing, and biosensing. However, the current widely-used nanofabrication technologies are complicated, time-consuming and expensive. Fundamental research of electrokinetic transport phenomena at the nanoscale has been focused on analytical and numerical models, and very limited experimental studies have been conducted. Resistive pulse sensing (RPS) technology has been integrated with nanofluidics and applied in nanoparticle characterization; however, traditional nano-orifice-based RPS detection systems are not suitable for practical applications due to their bulky size, low sensitivity, and high cost. This thesis studies electrokinetic transport phenomena in polydimethylsiloxane (PDMS) nanochannels, as well as applications of nanochannel-based nanofluidic devices in nanoparticle detection and molecule sensing. At the beginning of this thesis, a cost-effective, productive and simple method for fabricating disposable PDMS nanofluidic devices by the solvent-induced cracking method and nanoimprint technique is developed. The channel size is controlled by the working parameters of the solvent-induced cracking method while the quantity and locations of these nanocracks are determined by artificial defects. A detailed guideline for making PDMS nanofluidic chips with single nanochannels or multiple nanochannels of controllable channel size and spacing is provided. Nanochannels of 20 nm in depth can be obtained easily by using this method. Two fundamental research projects are conducted on single-nanochannel-based nanofluidic chips to investigate electrokinetically driven fluids and particles in nanochannels. Electroosmotic flow (EOF) in single nanochannels is measured by the current slope method. Channel size effects, concentration effects and electric field effects upon EOF velocity in nanochannels are investigated systematically. The decrease of EOF velocity due to overlapping of electric double layers (EDLs) is demonstrated by experiments. The experimental results are in good agreement with the numerical simulation results. Electrokinetic (EK) motion of single nanoparticles in single PDMS nanochannels is investigated by particle tracing method systematically. Effects of ionic concentration of the electrolyte media, applied electric field, and particle-to-channel size ratio on particle velocity are studied. The velocity of nanoparticles inside the single nanochannels is suppressed by the confined nanospace due to interactions between EDLs. Lastly, by using the nanochannel-based nanofluidic chips, two applications are developed to detect nanoparticles, cations as well as DNA molecules by the RPS technique. A mathematic model for single-gate differential RPS detection systems is developed to evaluate the RPS signals; working parameters involved in particle detection by using the nanochannel-based differential RPS chips are studied experimentally. The signal-to-noise ratio (SNR) of the PDMS nanochannel-based differential RPS systems is also explored by experiments. To enhance the resolution of the nanochannel-based RPS devices, carbon nanotubes (CNTs) are integrated into differential RPS nanofluidic chips to work as the sensing gates. Novel methods to isolate and cut individual CNTs for CNT-based nanofluidic devices are also created. The CNT-channel-based differential RPS chips are used to detect single cations and individual ssDNA molecules. Distinguishing of 15-nt ssDNAs and 30-nt ssDNAs with high resolution has been achieved. This thesis provides the nanofluidic research community with a comprehensive working procedure for fabricating cost-effective PDMS-based nanofluidic chips. The fundamental studies in this thesis expand our understanding of electrokinetic transport phenomena at the nanoscale, and the differential RPS detection systems developed on the nanochannel-based nanofluidic chips open a new avenue to nanoparticle detection as well as molecule sensing.

Download or read book Electrokinetic Trapping of Biomolecules written by Ying-Chih Wang and published by . This book was released on 2007 with total page 141 pages. Available in PDF, EPUB and Kindle. Book excerpt: (Cont.) By manipulating analyte concentrations, this integrated device not only increases the detection sensitivity, but also expands the dynamic range of given antibody-antigen couples. In addition, we also investigated the ion transfer at the micro-/nano-fluidic interface. Depending on the strength of the applied electric field across the nanochannel array, various phenomena such as concentration polarization, charge depletion, and nonlinear electrokinetic flows in the adjacent microfluidic channel can be observed and studied in situ by fluorescent microscopy. In summary, the nanofluidic concentrator we developed in this thesis facilitates sample preparation and detection of biomolecules from complex biological matrices and facilitates a further understanding of nanoscale molecular/fluid/ion transport phenomena by providing a well-controlled experimental platform.

Download or read book Electrokinetic Particle Transport in Micro Nanofluidics written by Shizhi Qian and published by CRC Press. This book was released on 2012-06-19 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: Numerous applications of micro-/nanofluidics are related to particle transport in micro-/nanoscale channels, and electrokinetics has proved to be one of the most promising tools to manipulate particles in micro/nanofluidics. Therefore, a comprehensive understanding of electrokinetic particle transport in micro-/nanoscale channels is crucial to the development of micro-/nanofluidic devices. Electrokinetic Particle Transport in Micro-/Nanofluidics: Direct Numerical Simulation Analysis provides a fundamental understanding of electrokinetic particle transport in micro-/nanofluidics involving electrophoresis, dielectrophoresis, electroosmosis, and induced-charge electroosmosis. The book emphasizes the direct numerical simulation of electrokinetic particle transport phenomena, plus several supportive experimental studies. Using the commercial finite element package COMSOL Multiphysics®, it guides researchers on how to predict the particle transport subjected to electric fields in micro-/nanoscale channels. Researchers in the micro-/nanofluidics community, who may have limited experience in writing their own codes for numerical simulations, can extend the numerical models and codes presented in this book to their own research and guide the development of real micro-/nanofluidics devices. Corresponding COMSOL® script files are provided with the book and can be downloaded from the author’s website.

Download or read book Micro Nano Chip Electrokinetics Volume III written by Xiangchun Xuan and published by MDPI. This book was released on 2021-02-24 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt: Micro/nanofluidics-based lab-on-a-chip devices have found extensive applications in the analysis of chemical and biological samples over the past two decades. Electrokinetics is the method of choice in these micro/nano-chips for transporting, manipulating, and sensing various analyte species (e.g., ions, molecules, fluids, and particles). This book aims to highlight the recent developments in the field of micro/nano-chip electrokinetics, ranging from the fundamentals of electrokinetics to the applications of electrokinetics to both chemo- and bio-sample handling.

Download or read book Essentials of Micro and Nanofluidics written by A. Terrence Conlisk and published by Cambridge University Press. This book was released on 2012-12-24 with total page 559 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook introduces students to the basic physical principles to analyse fluid flow in micro- and nano-size devices. This is the first book that unifies the thermal sciences with electrostatics and electrokinetics and colloid science; electrochemistry; and molecular biology. Key concepts and principles are discussed, such as the essentials of viscous flows, introductory electrochemistry, heat and mass transfer phenomena, elements of molecular and cell biology and much more. State-of-the-art analytical and computational approaches to problems in all of these areas are presented, especially electrokinetic flows, and examples are given of the use of these approaches to design devices used for rapid molecular analysis, biochemical sensing, drug delivery, DNA analysis, the design of an artificial kidney and other transport phenomena. There are exercise problems and modern examples of applications, as well as a solutions manual available for qualified instructors.

Download or read book Nonlinear Electrokinetic Transport and Its Applications Under Combined AC and DC Fields in Micro nanofluidic Interface Devices written by and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Electrokinetic Transport Phenomena in Nanofluidic Devices written by Mingkan Zhang and published by . This book was released on 2012 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Active Electrokinetic Transport Control in a Nanofluidic Device with Embedded Surface Electrodes written by Marie Fuest and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the 1990s, lab on-a-chip (LoC) systems, or miniaturized devices that perform several unit operations that typically require bench top sized equipment on an integrated platform have been developed for applications in biotechnology, chemical and biological reactors, energy conversion systems, separation and filtration systems, and medical, pharmaceutical, and environmental monitoring. The success of LoC technology relies on the ability to manipulate ions and molecules in increasingly small volumes of fluid (nL and less). With decreasing critical length scales for devices made possible by advances in microfabrication and nanofabrication, the device surface-area-to volume ratio has made the role of surface properties critical to operation and novel functionality of many of these devices. Of the various surface properties, surface charge presents a unique parameter with broad applicability for engineering new device functionalities.

Download or read book Modeling Electrokinetic Phenomana in Micro and Nanofluidic Technology written by Shizhi Qian and published by Wiley. This book was released on 2013-04-30 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on modeling electrokinetic phenomena, including electroosmosis, electrophoresis, and dielectrophoresis in micro- and nanofluidic devices, which play important roles in facilitating fluid transport and particle manipulation in micro- and nanofluidic devices. These have the potential to improve and reduce the cost of healthcare around the globe and revolutionize chemical and biological analysis by integrating and automating sophisticated diagnostic procedures in micro/nanofluidic devices. This high-level treatment offers an essential guide for researchers and advanced students in nanotechnology, mechanical engineering, and colloid science.

Download or read book Single Nanopore Transport of Synthetic and Biological Polyelectrolytes in Three dimensional Hybrid Microfluidic nanofluidic Devices written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This paper presents a study of electrokinetic transport in single nanopores integrated into vertically-stacked three-dimensional hybrid microfluidic/nanofluidic structures. In these devices single nanopores, created by focused ion beam (FIB) milling in thin polymer films, provide fluidic connection between two vertically separated, perpendicular microfluidic channels. Experiments address both systems in which the nanoporous membrane is composed of the same (homojunction) or different (heterojunction) polymer as the microfluidic channels. These devices are then used to study the electrokinetic transport properties of synthetic (i.e., polystyrene sulfonate and polyallylamine) and biological (i.e., DNA) polyelectrolytes across these nanopores. Single nanopore transport of polyelectrolytes across these nanopores using both electrical current measurements and confocal microscopy. Both optical and electrical measurements indicate that electroosmotic transport is predominant over electrophoresis in single nanopores with d> 180 nm, consistent with results obtained under similar conditions for nanocapillary array membranes.

Download or read book Physicochemical Hydrodynamics written by Ronald F. Probstein and published by Butterworth-Heinemann. This book was released on 2013-10-22 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Physicochemical Hydrodynamics: An Introduction aims to provide an introduction to physicochemical hydrodynamics (PCH), which deals with the interaction between fluid flow and physical, chemical, and biochemical processes. PCH has applications in many areas of science and technology and is a rapidly expanding field. Emphasis of this book is on rational theory and its consequences, with the purpose of showing the underlying unity of PCH, in which diverse phenomena can be described in physically and mathematically similar ways. The magic of this unity is shown in the similar manner in which solutes concentrate in a flow containing chemically reacting surfaces, reverse osmosis membranes, and electrodialysis membranes or the similarity of particle motions in sedimentation, centrifugation, ultrafiltration, and electrophoresis. Problems are included at the end of each chapter and are ordered following the sequence in which the material is set out. The questions range in difficulty, with most requiring an analytic development, but with some asking only for a descriptive answer. All problems are intended to illustrate the ideas presented, though often the solution goes beyond the explicit discussion in the book, with the answer constituting a generalization or extension of the text material. This book is an essential source of information for students majoring in the field of PCH.

Download or read book Micro and Nanoscale Fluid Mechanics written by Brian J. Kirby and published by Cambridge University Press. This book was released on 2010-07-26 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text focuses on the physics of fluid transport in micro- and nanofabricated liquid-phase systems, with consideration of gas bubbles, solid particles, and macromolecules. This text was designed with the goal of bringing together several areas that are often taught separately - namely, fluid mechanics, electrodynamics, and interfacial chemistry and electrochemistry - with a focused goal of preparing the modern microfluidics researcher to analyse and model continuum fluid mechanical systems encountered when working with micro- and nanofabricated devices. This text serves as a useful reference for practising researchers but is designed primarily for classroom instruction. Worked sample problems are included throughout to assist the student, and exercises at the end of each chapter help facilitate class learning.

Download or read book Diagnostic Molecular Biology written by Chang-Hui Shen and published by Elsevier. This book was released on 2023-06-29 with total page 590 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diagnostic Molecular Biology, Second Edition describes the fundamentals of molecular biology in a clear, concise manner with each technique explained within its conceptual framework and current applications of clinical laboratory techniques comprehensively covered. This targeted approach covers the principles of molecular biology, including basic knowledge of nucleic acids, proteins and chromosomes; the basic techniques and instrumentations commonly used in the field of molecular biology, including detailed procedures and explanations; and the applications of the principles and techniques currently employed in the clinical laboratory. Topics such as whole exome sequencing, whole genome sequencing, RNA-seq, and ChIP-seq round out the discussion. Fully updated, this new edition adds recent advances in the detection of respiratory virus infections in humans, like influenza, RSV, hAdV, hRV but also corona. This book expands the discussion on NGS application and its role in future precision medicine. Provides explanations on how techniques are used to diagnosis at the molecular level Explains how to use information technology to communicate and assess results in the lab Enhances our understanding of fundamental molecular biology and places techniques in context Places protocols into context with practical applications Includes extra chapters on respiratory viruses (Corona)

Download or read book Chemical Imaging Analysis written by Freddy Adams and published by Elsevier. This book was released on 2015-06-06 with total page 493 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemical Imaging Analysis covers the advancements made over the last 50 years in chemical imaging analysis, including different analytical techniques and the ways they were developed and refined to link the composition and structure of manmade and natural materials at the nano/micro scale to the functional behavior at the macroscopic scale. In a development process that started in the early 1960s, a variety of specialized analytical techniques was developed – or adapted from existing techniques – and these techniques have matured into versatile and powerful tools for visualizing structural and compositional heterogeneity. This text explores that journey, providing a general overview of imaging techniques in diverse fields, including mass spectrometry, optical spectrometry including X-rays, electron microscopy, and beam techniques. Provides comprehensive coverage of analytical techniques used in chemical imaging analysis Explores a variety of specialized techniques Provides a general overview of imaging techniques in diverse fields

Download or read book Nanofluidics and Microfluidics written by Shaurya Prakash and published by William Andrew. This book was released on 2014-01-16 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: To provide an interdisciplinary readership with the necessary toolkit to work with micro- and nanofluidics, this book provides basic theory, fundamentals of microfabrication, advanced fabrication methods, device characterization methods and detailed examples of applications of nanofluidics devices and systems. Case studies describing fabrication of complex micro- and nanoscale systems help the reader gain a practical understanding of developing and fabricating such systems. The resulting work covers the fundamentals, processes and applied challenges of functional engineered nanofluidic systems for a variety of different applications, including discussions of lab-on-chip, bio-related applications and emerging technologies for energy and environmental engineering. The fundamentals of micro- and nanofluidic systems and micro- and nanofabrication techniques provide readers from a variety of academic backgrounds with the understanding required to develop new systems and applications. Case studies introduce and illustrate state-of-the-art applications across areas, including lab-on-chip, energy and bio-based applications. Prakash and Yeom provide readers with an essential toolkit to take micro- and nanofluidic applications out of the research lab and into commercial and laboratory applications.

Download or read book Micro Nano Chip Electrokinetics written by Xiangchun Xuan and published by MDPI. This book was released on 2018-07-04 with total page 203 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "Micro/Nano-Chip Electrokinetics" that was published in Micromachines