Download or read book Elaboration of Fluorescent Molecular Probes and Molecular based Nanoparticles for Bioimaging Purposes written by Cristiano Matteo Mastrodonato and published by . This book was released on 2017 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence-based techniques are popular tools for the study and understanding of biological processes. This has prompted continuous research aimed at the development of a wide range of fluorescent probes specifically designed for specific applications. Among them, fluorescent pH probes are of much interest as pH variations or gradients are involved in many biological events and anomalous alterations are often related to the onset of dysfunctions and diseases. In this framework we have developed a series of promising two-photon pH fluorescent molecular probes. These quadrupolar bolaamphiphilic probes are of great interest, as they combine a steep pH dependence of their optical properties close to neutral pH, ratiometric behavior and large response to two-photon (2P) excitation in the NIR region. As such they offer much promise for ratiometric detection of the pH in biological environments and in situ monitoring of acidification. In parallel, we have been interest in the design of ultrabright nanoparticles for bioimaging purpose (in particular highly sensitive optical imaging). We chose to focus on Fluorescent Organic Nanoparticles made of organic molecules with low molecular weight (FONs) as they offer a flexible route and promising alternatives to toxic quantum dots. In this case the design of the dye used as building blocks of the FONs is of crucial importance and strongly influence the chemical and physical properties of the nanoparticles generated, such as their one and two-photon brightness and both their structural and colloidal stability. In that context a library of novel dipolar chromophores have been synthesized and used to prepare FONs using the nanoprecipitation method. Their properties were thoroughly investigated in order to determine the relationship between the molecular design of the isolated dye and the overall properties of the nanoparticles made of these dyes. As a result, Hyperbright FONs emitting in the green to NIR region and combining giant brightness and remarkable stability have been achieved. They offer major promise for bioimaging based on both excitation and detection in the NIR region.

Download or read book Near infrared Nanomaterials written by Professor Fan Zhang and published by Royal Society of Chemistry. This book was released on 2016 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last decade, bioimaging and therapy based on near-infrared (NIR) nanomaterials have played an important role in biotechnology due to their intrinsic advantages when compared with the traditional imaging probe and medicine. NIR nanomaterials allow deeper penetration depth, low detection threshold concentration and better targeted performance. This book systematically summarises the recent progress in the fabrication and application of NIR nanomaterials for biomedical imaging and therapy, and discusses the advantages, challenges and opportunities available. Near-infrared Nanomaterials contains a chapter highlighting the outlook of these materials, detailing novel ideas for the further application of NIR nanomaterials in bioimaging and medicine. Written by leading experts working in the field, this title will have broad appeal to those working in chemistry, materials science, nanotechnology, biology and bioengineering, biomedical science and biophysics.

Download or read book SMALL MOLECULE BASED FLUORESCENT MOLECULAR PROBES FOR FACILITATING BIOMEDICAL RESEARCH written by and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract : My thesis is focused on the development of fluorescent probes for biosensing and bioimaging within specific organelles. My main research efforts are mainly focused on the design, synthesis and biological applications of these new molecular probes. These new fluorescent probes I developed can be manipulated through the chemical modifications for binding to specific organelles capable of reporting localized bioinformation. Compared to the currently commercially available organelle-specific fluorescent stains, the advantages of the newly synthesized fluorescent probes include low cytotoxicity, high photostability, and long fluorescence lifetimes. These features are crucial for long-time tracking study of biological processes. Research on fluorescent probes with both analyte responsiveness and organelle targetability is a new and emerging area that has attracted increasing attention over the past few years. Because of their high sensitivity, specificity and fast response, these novel fluorescent probes have been proven to be useful tools for facilitating biomedical research. I have further extended the diversity by developing organelle-specific responsive probes capable of detecting changes in biomolecular levels and the microenvironment. My future research efforts give more considerations of the "low-concern" organelles, such as the Golgi apparatus, the endoplasmic reticulum, and ribosomes. Considering the tiny sizes of subcellular organelles, we anticipate that better visualization of the cellular events within specific organelles will rely on super-resolution optical microscopy with nanoscopic-scale resolution.

Download or read book Fluorescent Organic Nanoparticles written by Waseem A. Wani and published by Springer. This book was released on 2018-09-27 with total page 72 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a critical review of recent advances in the development of fluorescent organic nanoparticles as materials of choice for the design and fabrication of sensors, bioimaging agents and drug delivery systems. The properties and functions of nanoparticles differ significantly from those of their parent entities or their bulk phases. Two of their most important features are their increased surface-to volume ratio, and the formation of surface structures differing from those in their bulk phases. In addition, the book discusses the synthesis of fluorescent conjugated polymers, self-assembled fluorescent nanoparticles, polydopamine nanoparticles, and aggregation-induced-emission or aggregation-induced-emission enhancement nanomaterials. In closing, the book provides an outlook on future research and development in fluorescent organic nanoparticles as smart materials with an impressive range of potential applications.

Download or read book Fluorescent Energy Transfer Nucleic Acid Probes written by Vladimir V. Didenko and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. These probes signal detection of their targets by changing either the intensity or the color of their fluorescence. Not surpr- ingly, these luminous, multicolored probes carry more flashy names than their counterparts in the other fields of molecular biology. In recent years, fluor- cent probes and assays, which make use of energy transfer, have multiplied at a high rate and have found numerous applications. However, in spite of this explosive growth in the field, there are no manuals summarizing different p- tocols and fluorescent probe designs. In view of this, the main objective of Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is to provide such a collection. Oligonucleotides with one or several chromophore tags can form fluor- cent probes capable of energy transfer. Energy transport within the probe can occur via the resonance energy transfer mechanism, also called Förster tra- fer, or by non-Förster transfer mechanisms. Although the probes using Förster transfer were developed and used first, the later non-Förster-based probes, such as molecular beacons, now represent an attractive and widely used option. The term “fluorescent energy transfer probes” in the title of this book covers both Förster-based fluorescence resonance energy transfer (FRET) probes and probes using non-FRET mechanisms. Energy transfer probes serve as molecule-size sensors, changing their fluorescence upon detection of various DNA reactions.

Download or read book Design and Synthesis of Ultra bright Organic Nanoparticles ONPs for Bioimaging written by Paolo Pagano and published by . This book was released on 2017 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nowadays the use of bright luminescent nano-objects in biological environment is a topic that is gaining more and more importance, especially for biomedical applications such as imaging, the rapyand diagnostic. So far, numerous studies have been conducted with gold nanoparticles, silica nanoparticles (doped or functionalized with organic molecules), as well as semiconductor nanoparticles (quantum dots, i.e., QDs). However, most of these nanoparticles suffer from drawbacks (in terms of stability, biocompatibility, eco-toxicity or degradability). On the other hand, several nontoxic fluorescent molecular probes have been widely used, but most of the time their brightness remain modest in biological environments compared to QDs. Our idea is to engineer new organicchromophores with tunable emission wavelength (from visible to near infrared) for further preparation of organic fluorescent nanoparticles (so called FONs) that display giant one-photon and two-photonbrightness, as well as good colloidal and chemical stability, and suitable photostability for in vitro andin vivo imaging. As such, these FONs would represent interesting alternatives to QDs for use in bioimaging. This manuscript describes the synthesis and characterization of new classes of fluorescent molecules specifically engineered as building blocks for the fast preparation of such nanoparticles byself-aggregation in water. The FONs were fully characterized from both morphological and photophysical points of view and further used in bioimaging.

Download or read book Fluorescence Methods for Investigation of Living Cells and Microorganisms written by Natalia Grigoryeva and published by BoD – Books on Demand. This book was released on 2020-09 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms.

Download or read book Advanced Fluorescence Reporters in Chemistry and Biology II written by Alexander P. Demchenko and published by Springer Science & Business Media. This book was released on 2010-09-08 with total page 461 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions by numerous experts

Download or read book Systematic Exploration of Indolizine Based Small Fluorescent Molecules written by Youngjun Lee and published by Springer. This book was released on 2018-08-16 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis describes an in-depth study of an indolizine-based fluorophore, from understanding of its structure-photophysical property relationship to its application as a useful biological reporter. Organic fluorophores have been extensively used in the field of molecular biology owing to their excellent photophysical property, suitable cell permeability, and synthetic flexibility. Understanding of the structure-photophysical property relationship of a given fluorophore often paves the road to the development of valuable molecular probes to visualize and transcribe biological networks. In this thesis, respective chapters deal with molecular design, organic synthesis, structure-property analysis, and quantum-mechanical interpretation of unexplored family of indolizine-based molecules. This systematic exploration has led to rational development of a new microalgae lipid droplet probe, colorful bioorthogonal fluorogenic probes, and a bright mitochondrial probe, working under live cell conditions. Harnessing the optical properties of a given fluorophore has been an important topic for a couple of decades, both in industry and in academia. This thesis provides useful insights for the improvement and development of unique small fluorescent materials, or optical materials.

Download or read book Functional Fluorescent Materials written by Vivek Mishra and published by . This book was released on 2024-06 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Fluorescence in Bio inspired Nanotechnology written by Jonas Hannestad and published by Springer Science & Business Media. This book was released on 2013-07-20 with total page 127 pages. Available in PDF, EPUB and Kindle. Book excerpt: In his thesis Fluorescence in Bio-inspired Nanotechnology, Jonas Hannestad describes the evolving field of DNA nanotechnology in a lucid and easily accessible way. A central theme in the thesis is how biological structures and mechanisms constitute a basis for the design of novel technologies. Hannestad discusses how self-assembled, nanometer-scale DNA constructs can be functionalized using fluorescent labeling. In particular, he highlights how applications are based on fluorescence resonance energy transfer (FRET). Another important contribution is the development of a lipid monolayer platform for the step-by-step assembly of DNA nanoconstructs. The work in the thesis is based on five peer-reviewed papers published in high-profile journals, all of which involve major contributions from the author.

Download or read book Silica Nanoparticle Platform to Generate New Molecular Probes for Fluorescence Lifetime Imaging written by Soo Young Song and published by . This book was released on 2016 with total page 39 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence microscopy is the most widely used detection method to image molecular probes, but is currently hindered due to the limited number of fluorescent probes available. The fluorescence lifetime of fluorescent probes that is the time an excited fluorophore takes to return to the ground state; this thesis studied a novel system to manipulate the fluorescent molecules' lifetime using the nanoprobe format. This system can exponentially increase the number of fluorescent probes. The first part of this thesis reports ̃130nm silica nanoparticles labeled with silanized fluorescent dyes -- Oregon Green 488, BODIPY, and rhodamine -- using various total dye loadings. As the total dye loading increased, the relative fluorescence efficiency decreased due to self-quenching. This self-quenching effect was characterized by the phasor approach to fluorescence lifetime that gave unique and distinguishable spatial values of the nanoparticles. Utilizing the synthetic versatile properties of silica in accommodating other materials, the second part of this thesis discussed core-shell structured silica nanoparticles with an independent control to the fluorescence lifetimes of the cores and the shells. 35nm Silica cores were synthesized using a reverse microemulsion method. Various concentrations of fluorescent nanomaterials encapsulated inside the cores govern the cores' phasor values. Subsequently, silica shells with various thicknesses were synthesized using the Stober sol-gel reaction. Various total dye loadings govern the shells' phasor values while the shell thicknesses control their fluorescence intensity. The distinct fluorescence lifetime phasor values enable nanoprobes to have visually effective representations that can be easily expanded by simply manipulating the molecular proximities between fluorophores.

Download or read book Metal Enhanced Fluorescence written by Chris D. Geddes and published by John Wiley & Sons. This book was released on 2010-06-22 with total page 655 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence.

Download or read book Carbon Nanomaterials for Bioimaging Bioanalysis and Therapy written by Yuen Y. Hui and published by John Wiley & Sons. This book was released on 2018-11-13 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive reference on biochemistry, bioimaging, bioanalysis, and therapeutic applications of carbon nanomaterials Carbon nanomaterials have been widely applied for biomedical applications in the past few decades, because of their unique physical properties, versatile functionalization chemistry, and biological compatibility. This book provides background knowledge at the entry level into the biomedical applications of carbon nanomaterials, focusing on three applications: bioimaging, bioanalysis, and therapy. Carbon Nanomaterials for Bioimaging, Bioanalysis and Therapy begins with a general introduction to carbon nanomaterials for biomedical applications, including a discussion about the pros and cons of various carbon nanomaterials for the respective therapeutic applications. It then goes on to cover fluorescence imaging; deep tissue imaging; photoacoustic imaging; pre-clinical/clinical bioimaging applications; carbon nanomaterial sensors for cancer and disease diagnosis; targeted cancer therapy; and photothermal/photodynamic therapy. Each chapter briefly introduces the biomedical application and emphasizes the most appropriate carbon nanomaterial(s) for the application. Provides an introduction to the biomedical applications of carbon nanomaterials for early-career scientists, as well as background and context for mid-career scientists and researchers Contains four sections covering biochemistry, bioimaging, bioanalysis, and therapeutic applications of carbon nanomaterials Presented by experts who have strong background in the field of nanotechnology for biomedical applications Covers a hot area of research which has very unique physical properties, versatile functionalization chemistry, and biological compatibility Carbon Nanomaterials for Bioimaging, Bioanalysis and Therapy is an excellent resource for academic researchers and industrial scientists working on preparation and bio-application of carbon nanomaterials, biomedical engineering, and nanotechnology.

Download or read book Fluorescent Nanodiamonds written by Huan-Cheng Chang and published by John Wiley & Sons. This book was released on 2018-11-12 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: The most comprehensive reference on fluorescent nanodiamond physical and chemical properties and contemporary applications Fluorescent nanodiamonds (FNDs) have drawn a great deal of attention over the past several years, and their applications and development potential are proving to be manifold and vast. The first and only book of its kind, Fluorescent Nanodiamonds is a comprehensive guide to the basic science and technical information needed to fully understand the fundamentals of FNDs and their potential applications across an array of domains. In demonstrating the importance of FNDs in biological applications, the authors bring together all relevant chemistry, physics, materials science and biology. Nanodiamonds are produced by powerful cataclysmic events such as explosions, volcanic eruptions and meteorite impacts. They also can be created in the lab by high-pressure high-temperature treatment of graphite or detonating an explosive in a reactor vessel. A single imperfection can give a nanodiamond a specific, isolated color center which allows it to function as a single, trapped atom. Much smaller than the thickness of a human hair, a nanodiamond can have a huge surface area that allows it to bond with a variety of other materials. Because of their non-toxicity, nanodiamonds may be useful in biomedical applications, such as drug delivery and gene therapy. The most comprehensive reference on a topic of rapidly increasing interest among academic and industrial researchers across an array of fields Includes numerous case studies and practical examples from many areas of research and industrial applications, as well as fascinating and instructive historical perspectives Each chapter addresses, in-depth, a single integral topic including the fundamental properties, synthesis, mechanisms and functionalisation of FNDs The first book published by the key patent holder with his research group in the field of FNDs Fluorescent Nanodiamonds is an important working resource for a broad range of scientists and engineers in industry and academia. It will also be a welcome reference for instructors in chemistry, physics, materials science, biology and related fields.

Download or read book Development and Applications of Novel Fluorescent Molecular Probe Strategies written by Dolonchampa Maji and published by . This book was released on 2017 with total page 149 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical imaging and spectroscopy technologies offer the ability to provide structural and functional information in a fast, low-cost, ionizing radiation free, highly sensitive and high throughput fashion. The diverse contrast mechanisms and complementary imaging platforms form the foundation for the application of optical imaging in pre-clinical studies of pathophysiological development as well as direct clinical application as a tool for diagnosis and therapy. Fluorescence imaging techniques have been one of the most rapidly adopted methods in biology and biomedicine. Visualization of biological processes and pathologic conditions at the cellular and tissue levels largely relies on the use of exogenous fluorophores or their bioconjugates. Some fluorescent molecular probes provide usable contrast for disease diagnosis due to their responsiveness to interactions with other molecular species and/or immediate microenvironment. As a result, understanding exogenous fluorescent contrast mechanisms will allow the development of efficient strategies for biomedical fluorescence imaging. The present work focuses on exploring novel fluorescent molecular probe strategies for imaging cancer and cardiovascular diseases. We have developed a platform for synthesizing activatable fluorescent molecular probes using the fluorescence quenching properties of copper (II) ions. We used these activatable probes for rapid imaging of cancerous tissue in vivo in mice. While developing these molecular probes, we discovered an unexpected molecular interaction that yields stable dimeric molecules. This finding can potentially enable the development of new molecular entities for modifying the signaling properties of fluorescent dyes to minimize background fluorescence. Although planar fluorescence imaging methods using exogenous molecular probes provide rapid information about molecular processes in vivo, extraction of depth information require complex data acquisition and image analysis methods. By designing a dual emission fluorescent probe incorporating two spectrally different fluorophore systems, we developed a method to successfully estimate the depth of fluorescent inclusions from planar imaging data and demonstrated the potential of using this approach to locate a blood vessel and tumorous tissue in mouse in vivo. An important feature of fluorescence methods is the availability of various techniques that provide complementary information. Combining the fluorescence intensity and lifetime properties of a biologically targeted near infrared fluorescent probe, we demonstrate an effective way to distinguish specific from nonspecific uptake mechanisms in cancer cells, an approach that can be translated in vivo. Alternatively, dynamic fluorescence imaging technique expands the scope of applications to include detection and estimation of the size of circulating cancer cells and clusters. The approach developed in this work could allow longitudinal monitoring of these cells, which are implicated in cancer metastases. To circumvent the shallow penetration of light using optical methods, we developed multimodal imaging approaches by incorporating a radionuclide for nuclear imaging into a broad spectrum near infrared fluorescent tumor targeting agent. This molecular construct allows for noninvasive whole body nuclear imaging of tumors, followed by fluorescence image guided resection. In each of these areas, novel fluorescent molecular probes were developed, characterized and applied to solve critical biomedical problems.

Download or read book Identification of Biological Species Using Fluorescent Molecular Probes written by Poojitha B Sridhara Setty and published by . This book was released on 2024-05-04 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: According to popular belief, appealing to the sense of sight is more powerful than appealing to the sense of hearing, which could be the origin of the adage ""Seeing is believing."" Technologies that allow researchers to ""see into the body"" or ""see into cells"" are crucial for both basic biological processes research and the detection and treatment of disease. It is ideal for the procedures to be non-invasive, meaning they shouldn't include slicing into the body or separating its components. Thus, in the biomedical sciences, methods for visualising physiological or pathological changes in the body and cells have grown in significance. Contributing to the regulation of numerous physiological processes and playing critical roles in preserving the healthy development of living bodies, a few significant biological species and their microenvironments manage a complex and sensitive dynamic balance in life systems. Living things can develop a variety of illnesses or even pass away if their homeostasis is disturbed. Real-time monitoring of these biological species and their microenvironments during various physiological and pathological processes is therefore extremely important. One of the most effective methods for real-time imaging in biological samples is the use of fluorescent probe-based approaches. Small-molecule fluorescent probes have developed into effective instruments for exploiting light to enhance the field of cell biology research, find novel medications, identify pollutants in the environment, and increase cancer diagnosis. The growth of the fluorescent probe research community-which was tiny in the late 20th century but now comprises over a hundred research groups worldwide-correlates with these uses. It took scientists with a lot of courage from many different professions to join this expansion. In this book, pyrene, coumarin, and naphthalimide are among the fluorescent dyes that are most frequently employed for fluorescence imaging.