Download or read book DNA Topoisomerase Protocols written by Neil Osheroff and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 331 pages. Available in PDF, EPUB and Kindle. Book excerpt: Beginning with the Escherichia coli ? protein, or bacterial DNA topoisomerase I, an ever-increasing number of enzymes have been identified that catalyze changes in the linkage of DNA strands. DNA topoisomerases are ubiquitous in nature and have been shown to play critical roles in most p- cesses involving DNA, including DNA replication, transcription, and rec- bination. These enzymes further constitute the cellular targets of a number of clinically important antibacterial and anticancer agents. Thus, further studies of DNA topology and DNA topoisomerases are critical to advance our und- standing of the basic biological processes required for cell cycle progression, cell division, genomic stability, and development. In addition, these studies will continue to provide critical insights into the cytotoxic action of drugs that target DNA topoisomerases. Such mechanistic studies have already played an important role in the development and clinical application of antimicrobial and chemotherapeutic agents. The two volumes of DNA Topoisomerase Protocols are designed to help new and established researchers investigate all aspects of DNA topology and the function of these enzymes. The chapters are written by prominent investigators in the field and provide detailed background information and st- by-step experimental protocols. The topics covered in Part I: DNA Topology and Enzymes, range from detailed methods to analyze various aspects of DNA structure, from linking number, knotting/unknotting, site-specific recombi- tion, and decatenation to the overexpression and purification of bacterial and eukaryotic DNA topoisomerases from a variety of cell systems and tissues.
Download or read book DNA Topoisomerase Protocols written by Mary-Ann Bjornsti and published by Humana. This book was released on 1999-03-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Beginning with the Escherichia coli co protein, or bacterial DNA topoisomerase I, an ever-increasing number of enzymes has been identified that catalyze changes in the linkage of DNA strands. DNA topoisomerases are ubiquitous in nature and have been shown to play critical roles in most p- cesses involving DNA, including DNA replication, transcription, and rec- bination. These enzymes further constitute the cellular targets of a number of clinically important antibacterial and anticancer agents. Thus, further studies of DNA topology and DNA topoisomerases are critical to advance our und- standing of the basic biological processes required for cell cycle progression, cell division, genomic stability, and development. In addition, these studies will continue to provide critical insights into the cytofoxic action of drugs that target DNA topoisomerases. Such mechanistic studies have already played an important role in the development and clinical application of antimicrobial and chemotherapeutic agents. The two volumes of DNA Topoisomerase Protocols are designed to help new and established researchers investigate all aspects of DNA topology and the function of these enzymes. The chapters are written by prominent investigators in the field and provide detailed background information and st- by-step experimental protocols. The topics covered in Volume I; DNA Topology and Enzymes, range from detailed methods to analyze various aspects of DNA structure, from linking number, knotting/unknotting, site-specific recombi- tion, and decatenation to the overexpression and purification of bacterial and eukaryotic DNA topoisomerases from a variety of cell systems and tissues.
Download or read book DNA Topoisomerase Protocols written by Neil Osheroff and published by . This book was released on 1999 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book DNA Topoisomerase Protocols written by Mary-Ann Bjornsti and published by . This book was released on 1999 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book DNA Topoisomerases written by Duncan J. Clarke and published by Methods in Molecular Biology. This book was released on 2009-09-25 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA topoisomerases and gyrases have been studied thoroughly from a biochemical perspective, and expanding research at the molecular and cellular level shows great promise. This book gives a compendium of protocols that extends into such new areas of research.
Download or read book DNA Topoisomerase Protocols written by Mary-Ann Bjornsti and published by . This book was released on 1999 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book DNA Topoisomerase Protocols written by Mary-Ann Bjornsti and published by Humana. This book was released on 1999-03-23 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: Beginning with the Escherichia coli co protein, or bacterial DNA topoisomerase I, an ever-increasing number of enzymes has been identified that catalyze changes in the linkage of DNA strands. DNA topoisomerases are ubiquitous in nature and have been shown to play critical roles in most p- cesses involving DNA, including DNA replication, transcription, and rec- bination. These enzymes further constitute the cellular targets of a number of clinically important antibacterial and anticancer agents. Thus, further studies of DNA topology and DNA topoisomerases are critical to advance our und- standing of the basic biological processes required for cell cycle progression, cell division, genomic stability, and development. In addition, these studies will continue to provide critical insights into the cytofoxic action of drugs that target DNA topoisomerases. Such mechanistic studies have already played an important role in the development and clinical application of antimicrobial and chemotherapeutic agents. The two volumes of DNA Topoisomerase Protocols are designed to help new and established researchers investigate all aspects of DNA topology and the function of these enzymes. The chapters are written by prominent investigators in the field and provide detailed background information and st- by-step experimental protocols. The topics covered in Volume I; DNA Topology and Enzymes, range from detailed methods to analyze various aspects of DNA structure, from linking number, knotting/unknotting, site-specific recombi- tion, and decatenation to the overexpression and purification of bacterial and eukaryotic DNA topoisomerases from a variety of cell systems and tissues.
Download or read book DNA Topoisomerase Protocols written by Neil Osheroff and published by Humana. This book was released on 2000-10-20 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: Beginning with the Escherichia coli ? protein, or bacterial DNA topoisomerase I, an ever-increasing number of enzymes have been identified that catalyze changes in the linkage of DNA strands. DNA topoisomerases are ubiquitous in nature and have been shown to play critical roles in most p- cesses involving DNA, including DNA replication, transcription, and rec- bination. These enzymes further constitute the cellular targets of a number of clinically important antibacterial and anticancer agents. Thus, further studies of DNA topology and DNA topoisomerases are critical to advance our und- standing of the basic biological processes required for cell cycle progression, cell division, genomic stability, and development. In addition, these studies will continue to provide critical insights into the cytotoxic action of drugs that target DNA topoisomerases. Such mechanistic studies have already played an important role in the development and clinical application of antimicrobial and chemotherapeutic agents. The two volumes of DNA Topoisomerase Protocols are designed to help new and established researchers investigate all aspects of DNA topology and the function of these enzymes. The chapters are written by prominent investigators in the field and provide detailed background information and st- by-step experimental protocols. The topics covered in Part I: DNA Topology and Enzymes, range from detailed methods to analyze various aspects of DNA structure, from linking number, knotting/unknotting, site-specific recombi- tion, and decatenation to the overexpression and purification of bacterial and eukaryotic DNA topoisomerases from a variety of cell systems and tissues.
Download or read book Current Protocols in Pharmacology written by and published by . This book was released on 1998 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book DNA Topoisomerases written by Marc Drolet and published by Humana Press. This book was released on 2018-08-30 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume looks at the evolution of the topo field over the last year and discusses how DNA topoisomerases are directly and indirectly involved in numerous cellular processes. The chapters in this book cover various topics, such as type 1 topos with an evolutionary perspective; antimicrobial agents targeting topos; experimental protocols related to DNA topology, including a basic technique to measure DNA supercoiling in bacteria; protocols for in vitro studies, including assays to detect RNA topo activity; topo inhibitors; and a study of plasmid-mediated quinolone resistance in bacteria. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, DNA Topoisomerases: Methods and Protoc ols is intended for specialists and non-specialists with an overview of selected hot topics in the field, and the latest protocols to study DNA topology, topoisomerases functions and inhibition by various compounds.
Download or read book DNA Topoisomearases Biochemistry and Molecular Biology written by and published by Academic Press. This book was released on 1994-11-07 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: Each volume of Advances in Pharmacology provides a rich collection of reviews on timely topics. Emphasis is placed on the molecular basis of drug action, both applied and experimental.
Download or read book Forensic DNA Profiling Protocols written by Patrick J. Lincoln and published by Springer Science & Business Media. This book was released on 1998-01-22 with total page 617 pages. Available in PDF, EPUB and Kindle. Book excerpt: This state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The methods include the recovery of DNA from a large range of sample types, analysis of DNA as single and multi-locus VNTR probes, PCR amplification of STR and other loci, and mitochondrial sequencing. The expert scientists writing here -- many from laboratories around the world -- also discuss how to interpret the results in cases of unknown identity and disputed parentage.-- Covers all steps from extraction of human DNA through to analysis and interpretation-- Takes advantage of new methodologies such as capillary electrophoresis-- Clear step-by-step instructions ensure unfailing reproducibility.
Download or read book DNA Topology written by Andrew D. Bates and published by Oxford University Press, USA. This book was released on 2005 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: "A key aspect of DNA is its ability to form a variety of structures, this book explains the origins and importance of such structures"--Provided by publisher.
Download or read book Untangling the Double Helix written by James C. Wang and published by . This book was released on 2009 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: The problem of unraveling two intertwined strands during the duplication of DNA was recognized shortly after the proposal of the DNA double helix structure in 1953. A group of enzymes called DNA topoisomerases solve this problem by breaking and rejoining DNA molecules in a controlled manner, thereby allowing strands to be passed through each other and thus untangled—not just during DNA replication, but also during many other basic cellular processes. Because of their intimate involvement in the workings of the cell, topoisomerases are also the logical targets of many antibiotics (including Cipro) and anticancer agents. This book, written by James Wang, the discoverer of the first topoisomerase and a leader in the field since, presents ten chapters covering the historical backdrop of the DNA entanglement problem and the discovery of the DNA topoisomerases, how DNA topoisomerases perform their magic in DNA replication, transcription, genetic recombination and chromosome condensation, and how they are targets of therapeutic agents. The book should appeal to readers from undergraduates upwards with interests in the biological and clinical aspects of topoisomerase function, or in the mathematics and physics of topology.
Download or read book Eicosanoid Protocols written by Elias A Lianos and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Eicosanoid Protocols, Elias A. Lianos and a panel of hands-on experts present cutting-edge methods for the study of eicosanoids, including prostaglandins, thromboxanes, and leukotrienes. The readily reproducible methods described hereconcentrate on studying the regulation of expression and function of enzymes, particularly cyclooxygenase (and its two isoforms), phospholipase A2, and lipoxygenases involved in the synthesis of established eicosanoids. Additional chapters are devoted to the characterization and distribution of the thromboxane A2 receptor in tissues and the biological roles of novel eicosanoids. Timely and authoritative, the methods in this book will help their users in exploring the pathobiology of inflammation. Eicosanoid Protocols offers new and established researchers powerful, state-of-the-art tools to probe the regulation and function of eicosanoids.
Download or read book Antibiotic Resistance Methods and Protocols written by Stephen H. Gillespie and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: At a time of rising concern about drug resistance and falling output of new antibacterial compounds, antibiotic research has once again returned to the forefront of medical science. In Antibiotic Resistance: Methods and Protocols, Stephen Gillespie and a panel of leading clinical and diagnostic microbiologists describe a series of detailed molecular and physical methods designed to study the growing problem of antibiotic resistance, as well as facilitate new antibiotic research programs for its effective redress. The techniques range widely from those that provide rapid diagnosis via DNA amplification and phage display, to those for plotting the transmission of resistant organisms and investigating their epidemiology. The methods are readily adaptable to a wide range of resistant bacterial organisms. In order to ensure successful results, each method is described in minute detail and includes tips on avoiding pitfalls. Practical and wide-ranging, Antibiotic Resistance: Methods and Protocols provides a collection of indispensable techniques not only for illuminating the basic biology of antimicrobial resistance, but also for developing and implementing new diagnostic and epidemiological tools.
Download or read book Mitochondrial DNA written by William C. Copeland and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mutations within mitochondrial DNA (mtDNA) and the nuclear genes involved in the maintenance of mitochondrial DNA have been linked to a wide range of human diseases, including several of the most common diseases of aging. In Mitochondrial DNA: Methods and Protocols internationally recognized authorities describe in great detail the methods they have perfected to analyze mtDNA and the proteins involved in its maintenance. The analytical techniques cover the purification of mtDNA from a variety of sources and the analysis of DNA for both deletions, point mutations, and damage, for replication intermediates, and for following the fate of mtDNA outside of the mitochondria. Additional analytical methods are presented for analyzing the proteins and enzymes that maintain mtDNA. Each readily reproducible protocol includes step-by-step instructions, tips on avoiding pitfalls and extending the method to other situation, and introductory material explaining the theory behind the process. Comprehensive and timely, Mitochondrial DNA: Methods and Protocols offers both basic and clinical researchers proven cutting-edge methods for analyzing the role mtDNA plays in the aging process, apoptosis, and possibly some cancers, and for investigating the cause of mitochondrial dysfunction and disease.
