Download or read book DNA Sequencing and Analysis of an Olfactory specific Gene Cloned from the Olfactory System of Atlantic Salmon Salmo Salar written by Joan M. Lloyd and published by . This book was released on 2001 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation and Characterization of Atlantic Salmon Salmo Salar Olfactory Receptor Genes written by Jill Elizabeth Thorpe and published by . This book was released on 2000 with total page 94 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification Evolution and Expression of Three Olfactory Gene Families in Atlantic Salmon Salmo Salar written by Kimberley Anne Johnstone and published by . This book was released on 2011 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: It has been hypothesized that salmonids use olfactory cues to return to their natal rivers and streams. However, the key components of the molecular pathway involved in imprinting and homing are still unknown. If odorants are involved in salmon homing migration then olfactory receptors should play a critical role in the dissipation of information from the environment to the fish. To understand the molecular basis for imprinting and homing in Atlantic salmon (Salmo salar), it is important to identify and characterize the olfactory receptors in the Atlantic salmon genome. Aquatic chemical cues are received through the salmon nares and into the nasal cavity that contains a single olfactory organ, olfactory rosette. The olfactory rosette contains sensory neurons, which are thought to express only one olfactory receptor. In this study, three major superfamilies of fish olfactory receptors (MOR, ora and OlfC) were examined. To identify the olfactory genes in Atlantic salmon several genomic and bioinformatic techniques were used. First, an Atlantic salmon bacterial artificial chromosome (BAC) library was screened with probes designed from previously identified fish olfactory receptor sequences. Then a selected number of hybridization positive BACs containing olfactory receptors were shotgun cloned and sequenced. From these BAC sequences, two ora genes and 55 OlfC genes were identified in Atlantic salmon. The second technique used to identify olfactory receptors in Atlantic salmon was a bioinformatic approach that involved screening a 3-fold Atlantic salmon genome sequence for olfactory receptors. Using this approach, 24 MOR and the remaining five ora genes were identified, as well as another 24 partial genes or pseudogenes. As a first step to understand how olfactory receptors are involved in imprinting and homing, a suite of olfactory receptors were selected to examine the expression profiles of these genes across different life stages and life histories of wild Atlantic salmon from Newfoundland, Canada. Seven differentially expressed OlfC genes were identified in juvenile anadromous salmon compared to returning adult salmon. From this research, I hypothesize that OlfC genes may play an important role in the imprinting of home stream water olfactory cues in anadromous Atlantic salmon.
Download or read book Cloning and Expression Analysis of Atlantic Salmon Salmo Salar CYP1A written by Christopher Benjamin Rees and published by . This book was released on 2001 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification and Annotation of Full length Genes in Atlantic Salmon Salmo Salar written by Jong S. Leong and published by . This book was released on 2010 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Large-scale expressed sequence tags (ESTs) in Atlantic salmon (Salmo salar) are examined to answer questions regarding salmonid transcriptomes. ESTs represent raw and incomplete gene sequences that need to be read, assembled and analyzed with computer software. The goal of this thesis was to develop an automatically curated and publicly accessible set of annotated full-length genes, representing a near-complete transcript set for Salmo salar. In turn, these genes provide the framework for studies in gene expression, conservation, and molecular evolution. The work presented here also touches on the results of a molecular evolution study, as an example of how full-length gene identification can be used to answer biological questions. Previous to this study, a limited number of Atlantic salmon cDNA libraries and ESTs were known. To further the goal of determining complete gene sequences, highly enriched full-length cDNA libraries and full-length libraries were created and sequenced, resulting in the ability to identify a large number of full-length reference genes. Together, all libraries represent a diverse pool of transcriptome sequences for Salmo salar. The goal of producing an accurate large-scale full-length gene set on a duplicated genome is not trivial. Complete systems for this objective do not readily exist. EST sequencing, EST assembly, and data storage, are just a few of the initial computational issues that are addressed. Once these issues are resolved, the multi-step workflow of full-length gene determination is described. The final challenge involving the development of a concise and universally accessible system for visualization is discussed. The resulting computational framework that has been developed is shown to be able to handle the intricacies and the size of a duplicated salmonid genome. It has been largely accepted that Atlantic salmon have undergone a recent genome duplication. Gene paralogs provide one source of evidence for this event. Analysis of paralogs revealed signatures of asymmetric evolution possibly due to relaxation of selective pressure. This thesis provides a complete Bioinformatics analysis pipeline to analyze and to visualize a set of full-length reference genes for Atlantic salmon. Using full-length genes as a framework, the topic of molecular evolution was addressed to show evidence of asymmetrical evolution among gene duplicates. The full-length reference genes, along with ESTs and all putative transcripts, have been made publicly available. These results serve as a valuable genomic resource for next-generation sequencing and for all other salmonid research endeavours.
Download or read book Identification and Annotation of Full length Genes in Atlantic Salmon Salmo Salar written by Jong S. Leong and published by . This book was released on 2010 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Large-scale expressed sequence tags (ESTs) in Atlantic salmon (Salmo salar) are examined to answer questions regarding salmonid transcriptomes. ESTs represent raw and incomplete gene sequences that need to be read, assembled and analyzed with computer software. The goal of this thesis was to develop an automatically curated and publicly accessible set of annotated full-length genes, representing a near-complete transcript set for Salmo salar. In turn, these genes provide the framework for studies in gene expression, conservation, and molecular evolution. The work presented here also touches on the results of a molecular evolution study, as an example of how full-length gene identification can be used to answer biological questions. Previous to this study, a limited number of Atlantic salmon cDNA libraries and ESTs were known. To further the goal of determining complete gene sequences, highly enriched full-length cDNA libraries and full-length libraries were created and sequenced, resulting in the ability to identify a large number of full-length reference genes. Together, all libraries represent a diverse pool of transcriptome sequences for Salmo salar. The goal of producing an accurate large-scale full-length gene set on a duplicated genome is not trivial. Complete systems for this objective do not readily exist. EST sequencing, EST assembly, and data storage, are just a few of the initial computational issues that are addressed. Once these issues are resolved, the multi-step workflow of full-length gene determination is described. The final challenge involving the development of a concise and universally accessible system for visualization is discussed. The resulting computational framework that has been developed is shown to be able to handle the intricacies and the size of a duplicated salmonid genome. It has been largely accepted that Atlantic salmon have undergone a recent genome duplication. Gene paralogs provide one source of evidence for this event. Analysis of paralogs revealed signatures of asymmetric evolution possibly due to relaxation of selective pressure. This thesis provides a complete Bioinformatics analysis pipeline to analyze and to visualize a set of full-length reference genes for Atlantic salmon. Using full-length genes as a framework, the topic of molecular evolution was addressed to show evidence of asymmetrical evolution among gene duplicates. The full-length reference genes, along with ESTs and all putative transcripts, have been made publicly available. These results serve as a valuable genomic resource for next-generation sequencing and for all other salmonid research endeavours.
Download or read book The Fate of Duplicated Regions of the Atlantic Salmon Salmo Salar Genome microform written by Leslie Mitchell and published by Library and Archives Canada = Bibliothèque et Archives Canada. This book was released on 2004 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gene and genome duplications have played a major role in vertebrate evolution. Salmonids provide a useful resource for studying the consequences of these events as their common ancestor underwent a genome duplication between 25 and 120 miliion years ago. To understand how a genome reorganizes itself to cope with duplicated chromosomes and the importance of gene duplications for evolution and adaptation, homeologous regions of the Atlantic salmon genome were identified and studied within a large insert, genomic BAC library; these BACs contain the metallothionen event. A BAC from each region was subsequently shotgun subcloned and sequenced. Sequence analysis revealed the presence of 10 genes, retaining their collinearity between the BAcs, although pseudogenization events have occurred in one of the duplicate loci in two instances. Comparative genomic analysis revealed the existence of extraordinary conservation of syntney over time.
Download or read book Identification Evolution and Expression of the Trace Amine Associated Receptor TAAR Gene Family in Atlantic Salmon Salmo Salar written by Jordan Anthony Tessarolo and published by . This book was released on 2014 with total page 95 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is widely hypothesized that Atlantic salmon are imprinted at a young age with olfactory cues, which they use as a guide in order to return to their natal streams to spawn. However, the molecular mechanism(s) behind this biological phenomenon remain unknown. Therefore, in order to better understand imprinting and homing in Atlantic salmon, it is important to characterize the repertoire of olfactory receptors in thisspecies. A search of the first assembly of the Atlantic salmon genome revealed 27 putatively functional trace amine-associated receptor (TAAR) genes and 25 putative TAAR pseudo-genes. Genetic mapping, phylogenetic analysis, binding-site prediction, and quantitative PCR were performed using the Atlantic salmon TAAR genes. The identification of this gene family in Atlantic salmon will facilitate additional studiesinvolving olfaction and homing such as determining the range of allelic variation in olfactory receptors genes of different salmon populations.
Download or read book Olfaction written by Edgar Soria-Gómez and published by MDPI. This book was released on 2020-12-29 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: Our senses shape our reality and allow us to adapt to the everlasting changing environment. From all sensory modalities, olfaction is maybe the most intriguing one, probably because olfactory information influences our daily life without us even noticing. However, we can all relate to the powerful impact that the smell of our favorite food has on us. Likewise, olfactory cues could be determinants for partner selection (because love can be blind but not anosmic), mood regulation and cognition. Furthermore, recent studies link early olfactory dysfunctions to the occurrence of devastating pathologies, such as Alzheimer´s and Parkinson´s. Thus, the study of olfaction, at different levels from genetics to behavior, will pave the way for a better understanding of brain processes and associated disorders.
Download or read book The Molecular Basis of Smell and Taste Transduction written by Derek J. Chadwick and published by John Wiley & Sons. This book was released on 2008-04-30 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent application of the techniques of molecular biology and patch-clamp physiology has led to rapid advances in understanding the molecular events in chemosensory transduction. In this book, the latest results are presented and discussed by leading scientists. The extensive coverage encompasses many important topics, including mucous domains; microchemical heterogeneity in the mucociliary complex of the olfactory epithelium; membrane currents and mechanisms of olfactory transduction, and genetic and pathological taste variation.
Download or read book Olfactory Imprinting and Homing in Salmon written by A.D. Hasler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chance Favors Only the Prepared Mind How does a scientist go about the task of pushing back the curtains of the unknown? Certainly the romance of tackling the mysteries of nature provides the motivation, for who would not be inspired by the remarkable life history of this romantic beast, the salmon. After living in the Pacific Ocean for several years, salmon swim thousands of kilometers back to the stream of their birth to spawn. I have always been fascinated by the homing migration of salmon. Noone who has seen a 20-kilogram salmon fling itself into the air repeatedly until it is exhausted in a vain effort to surmount a waterfall can fail to marvel at the strength of the instinct that draws the salmon upriver to the stream where it was born. But how does it find its way back? I was puzzling over this problem during a family vacation in 1946. Inspired by the work of the great German Nobel Laureates, Karl von Frisch and Konrad Lorenz, I had been conducting research with my graduate student Theodore Walker, since 1945, on the ability of fishes to discriminate odors emanating from aquatic plants. Von Frisch had studied schooling minnows and discovered that, if broken, their skin emitted a con specific chemical substance, termed Schreckstoff, which caused other members of its school to disperse and hide.
Download or read book An in Situ Hybridization Study of the Olfactory Specific Clone OSC MRNA of the Olfactory Rosette of Atlantic Salmon written by Nicholas Beatty and published by . This book was released on 2004 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Development of the Olfactory System and the Regulation of Odorant Receptor Genes written by Alison Louise Barth and published by . This book was released on 1997 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Atlantic Salmon written by Eric Verspoor and published by John Wiley & Sons. This book was released on 2008-04-15 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Atlantic Salmon is a cultural icon throughout its North Atlantic range; it is the focus of probably the World’s highest profile recreational fishery and is the basis for one of the World’s largest aquaculture industries. Despite this, many wild stocks of salmon are in decline and underpinning this is a dearth of information on the nature and extent of population structuring and adaptive population differentiation, and its implications for species conservation. This important new book will go a long way to rectify this situation by providing a thorough review of the genetics of Atlantic salmon. Sponsored by the European Union and the Atlantic Salmon Trust, this book comprises the work of an international team of scientists, carefully integrated and edited to provide a landmark book of vital interest to all those working with Atlantic salmon.
Download or read book The Characterization of Repetitive DNA Elements in the Genome of Atlantic Salmon Salmo Salar written by John L. Goodier and published by . This book was released on 1994 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Olfactory Signal Transduction in Atlantic Salmon Salmo Salar written by Lee-Ju Cheng and published by . This book was released on 1994 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic Mapping in Atlantic Salmon Salmo Salar and Brown Trout Salmo Trutta written by Colin McGowan and published by . This book was released on 1996 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt:
