Download or read book DNA Helicase II and Exonuclease V of Escherichia Coli written by David Ronald Cavanagh and published by . This book was released on 1990 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Study Protein protein Interaction in Methyl directed DNA Mismatch Repair in E Coli Exonuclease I Exo I and DNA Helicas II UvrD A Minimal Exonuclease Domain of WRN Forms a Hexamer on DNA and Possesses Both 3 5 Exonuclease and 5 Protruding Strand Endonuclease Activities Solving the Structure of the Ligand Binding Domain of the Pregnane Xenobiotic Receptor with 17 Estradiol and written by and published by . This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Exonuclease I (ExoI) from Escherichia coli is a monomeric enzyme that processively degrades single stranded DNA in the 3' to 5' direction and has been implicated in DNA recombination and repair. It functions in numerous genome maintenance pathways, with particularly well defined roles in methyl-directed mismatch repair (MMR). The Escherichia coli MMR pathway can be reconstituted in vitro with the activities of eight proteins (8). MutS, MutL and MutH are involved in initiation of repair including mismatch recognition and generation of a nick at a nearby GATC sequence (53, 54, 55, 56). The hemimethylated state of GATC sequences immediately following replication serves as a signal to direct repair to the nascent strand of the DNA duplex (57, 58). DNA helicase II and one of several exonucleases (Exonucleas I, Exonuclease VII and RecJ) are required to excise the error-containing DNA strand beginning at the nicked GATC site (34, 35). Restoration of the correct DNA sequence by repair synthesis involves DNA polymerase III holoenzyme and SSB, and the final nick is sealed by DNA ligase (34). To identify interactions with ExoI involved in MMR repair system, we used the yeast two-hybrid system with ExoI as bait. By screening an E.coli genomic library, E. coli DNA helicase II (UvrD) was identified as a potential interacting protein. UvrD has been shown to be required for DNA excision repair, methyl-directed mismatch repair and has some undefined, role in DNA replication and recombination. In this report, in vitro experiments confirm that UvrD and ExoI make a direct physical interaction that may be required for function of the methyl-directed mismatch repair. Werner Syndrome is a rare autosomal recessive disease characterized by a premature aging phenotype, genomic instability and a dramatically increased incidence of cancer and heart disease. Mutations in a single gene encoding a 1,432 amino-acid helicase/exonuclease (hWRN) have been shown to be responsible for the development o

Download or read book Genetic Analysis of Escherichia Coli DNA Helicase II written by Enxin Deng and published by . This book was released on 1999 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Analysis of the Role of DNA Helicase II uvrD in DNA Replication in Escherichia Coli written by Sandeep Bhat and published by . This book was released on 1996 with total page 78 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic and Biochemical Analysis of Escherichia Coli DNA Helicase II written by Brian Keith Washburn and published by . This book was released on 1992 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Mechanistic Studies of the DNA Helicase II Protein of E coli written by Jaya G. Yodh and published by . This book was released on 1993 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic and Biochemical Analysis of DNA Helicase II Mutants of Escherichia Coli written by Gang Zhang and published by . This book was released on 1997 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book DNA Repair and Mutagenesis written by Errol C. Friedberg and published by American Society for Microbiology Press. This book was released on 2005-11-22 with total page 2587 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential resource for all scientists researching cellular responses to DNA damage. • Introduces important new material reflective of the major changes and developments that have occurred in the field over the last decade. • Discussed the field within a strong historical framework, and all aspects of biological responses to DNA damage are detailed. • Provides information on covering sources and consequences of DNA damage; correcting altered bases in DNA: DNA repair; DNA damage tolerance and mutagenesis; regulatory responses to DNA damage in eukaryotes; and disease states associated with defective biological responses to DNA damage.

Download or read book The Purification and Characterization of the Escherichia Coli Helicase II Protein and a Study of the Protein s Enzymatic Activities written by Gregory Thomas Runyon and published by . This book was released on 1991 with total page 620 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Protection of Parental T4 DNA from Degradation by Exonuclease V in E Coli written by Judith L. Silverstein and published by . This book was released on 1975 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Escherichia Coli DNA Helicase II written by Mark Christopher Hall and published by . This book was released on 1998 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Specificity of Escherichia Coli DNA Helicase II Binding to a DNA Ligand in the Presence and Absence of a Nucleotide Cofactor written by Marcy E. Latta and published by . This book was released on 1998 with total page 70 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Exonuclease V the RecBC Enzyme of Escherichia Coli written by Karen Martha Telander Muskavitch and published by . This book was released on 1981 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Recombinational Repair of DNA Damage written by Andrei Kuzminov and published by Landes Bioscience. This book was released on 1996 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Exonuclease V of Escherichia Coli written by Alan Storey and published by . This book was released on 1986 with total page 151 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book DNA Helicases and DNA Motor Proteins written by Maria Spies and published by Springer Science & Business Media. This book was released on 2012-11-19 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, a number of groundbreaking structural and mechanistic studies deepened our understanding of helicase mechanisms and established new approaches for their analyses. Many fundamental mechanistic questions ranging from the mechanism of force generation, mechanochemical coupling to distinct mechanisms by which the same enzyme translocates on DNA removing obstacles, unwinds DNA and/or remodels nucleoprotein complexes, however, remain to be answered. It is even less understood how the helicase motors are incorporated into a wide range of genome maintenance and repair machines. The field has reached a stage when the studies of molecular mechanisms and basic biology of helicases can and shall be integrated with the studies of development, cancer and longevity. The objective of this book is to provide the first systematic overview of structure, function and regulation of DNA helicases and related molecular motors. By integrating the knowledge obtained through the diverse technical approaches ranging from single-molecule biophysics to cellular and molecular biological studies the editors aim to provide a unified view on how helicases function in the cell, are regulated in response to different cellular stresses and are integrated into large macromolecular assemblies to form a complex and adaptive living system.