Download or read book Direction of DNA Replication in Escherichia Coli written by Raymond L. Rodriguez and published by . This book was released on 1974 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Regulation of Several Genes Involved in DNA Replication in Escherichia Coli written by Thomas David Daws and published by . This book was released on 1988 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Initiation of DNA Replication in Escherichia Coli written by Michael Dalbey and published by . This book was released on 1975 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Growth Rate Regulation and Control of Initiation of DNA Replication in Escherichia Coli written by Anne Eliane Chiaramello and published by . This book was released on 1990 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: The initiation of DNA synthesis at the chromosomal replication origin, oriC, in Escherichia coli involves an RNA polymerase-mediated step. The level of synthesis of transcripts moving counterclockwise toward oriC is controlled at two promoters, P1 (asnC) and P2 (mioC), and at two transcription terminator regions, T1 and T2. As shown by S1 mapping, termination at the T2 region occurs to the right of oriC at nucleotides 297-299 and 306-310, while major termination events in the T1 region occur in and near the mioC promoter. The majority of transcripts entering oriC originates from the mioC promoter. Transcription from the mioC promoter has been shown to enhance the frequency of initiation of DNA replication of oriC containing plasmids, and to stabilize these plasmids in the host cells. The mioC promoter, which is stringently controlled, is also growth rate regulated. The amount of mioC transcripts relative to the amount of total RNA was inversely correlated with growth rate. This transcript is characterized by a short half-life (1.5 min). The mioC promoter, which contains a DnaA protein binding site, was much less susceptible to repression by DnaA protein when located in the chromosome, than when located in a plasmid. Only a very high concentration of DnaA protein repressed the mioC promoter. The DnaA protein, which is required for initiation of DNA replication from oriC, is growth rate regulated. As shown by RNase protection, this regulation is exerted at the transcriptional level, affecting both promoters, dnaAp1 and dnaAp2. Transcription from these two promoters is also stringently controlled. The amount of DnaA protein in spoT mutants, which are deficient in ppGpp pyrophosphorylase activity, decreases as the severity in the mutation increases. Thus, the intracellular concentration of ppGpp influences the expression of the dnaA gene. In conclusion, the growth rate regulation and stringent control of the dnaA gene suggest that one way in which DNA replication is coordinated with the growth rate is via ppGpp synthesis at the ribosome.

Download or read book Initiation of the DNA Replication Cycle in Escherichia Coli written by Gretchen Herpel Stein and published by . This book was released on 1971 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book DNA Repair and Mutagenesis written by Errol C. Friedberg and published by American Society for Microbiology Press. This book was released on 2005-11-22 with total page 2587 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential resource for all scientists researching cellular responses to DNA damage. • Introduces important new material reflective of the major changes and developments that have occurred in the field over the last decade. • Discussed the field within a strong historical framework, and all aspects of biological responses to DNA damage are detailed. • Provides information on covering sources and consequences of DNA damage; correcting altered bases in DNA: DNA repair; DNA damage tolerance and mutagenesis; regulatory responses to DNA damage in eukaryotes; and disease states associated with defective biological responses to DNA damage.

Download or read book Interpretation of Ultraviolet stimulated DNA Replication in Escherichia Coli written by Priscilla Kensley Teichmann Cooper and published by . This book was released on 1971 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Microbiology written by Nina T. Parker and published by . This book was released on 2016-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology's art program enhances students' understanding of concepts through clear and effective illustrations, diagrams, and photographs.

Download or read book Cellular Contexts and Biochemical Mechanisms of Dna Replication Restart in Escherichia Coli written by Aidan Mair McKenzie and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Before cells can divide, they must first duplicate their genome through DNA replication. DNA replication is facilitated by the coordinated activities of multiprotein complexes (replisomes). While the speed and abilities of replisomes are mystifying, they are frequently met with challenges to their progression. During active replication, Escherichia coli replisomes are estimated to physically dissociate from the genome at least once per cell cycle after colliding with DNA damage or tightly associated proteins. To sustain cellular viability, DNA replication needs to be reinitiated at these abandoned replication forks through the process of DNA replication restart. Here, I present a genetic, biochemical, and structural investigation of DNA replication restart in E. coli to better define its cellular role and mechanistic details. In Chapter 1, I outline the original discovery of replication restart proteins (RRPs) and the evidence used to propose a three-pathway model for DNA replication restart in E. coli (consisting of the PriA/PriB, PriA/PriC, and PriC/Rep pathways). To facilitate replication restart, each pathway recognizes an abandoned replication fork, remodels the fork, recruits additional RRPs, and ultimately reloads the replicative helicase (DnaB) onto the DNA with the help of its helicase loader (DnaC). Chapter 2 describes a genetic study that systematically defined the roles of specific replication restart pathways in a variety of cellular environments. My results helped integrate DNA replication restart within other cellular processes by revealing a tight link between the repair of double-strand breaks and the PriA/PriB restart pathway. In Chapter 3, I exploited a streamlined DnaB reloading system afforded by PriC to structurally define its manner of binding to the replicative helicase/loader (DnaB6/DnaC6) complex. The cryoEM structure identified dramatic conformational changes required to satisfy the binding interface between PriC and DnaB, and subsequent biochemical investigation identified a hydrophobic packing interface as important for sustained binding. A summary of the findings in Chapters 2 and 3, as well as future directions, are provided in Chapter 4. This work probed mechanistic details of DNA replication restart in E. coli by revealing the cellular processes (and missteps) that necessitate replication restart and helped define the biochemical mechanism of PriC-mediated replicative helicase loading.

Download or read book Studies on DNA Replication in Escherichia Coli written by Paul H. Weigel and published by . This book was released on 1975 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book DNA Replication Control in Microbial Cell Factories written by Monika Glinkowska and published by Springer. This book was released on 2014-09-23 with total page 56 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work describes the current knowledge of biochemical mechanisms regulating initiation of DNA replication in Escherichia coli, which focuses on the control of activity of the DnaA protein. Examples of direct linkages between DNA replication and other cellular processes are provided. In addition, similarities of the mechanisms of regulation of DNA replication operating in prokaryotic and eukaryotic cells are identified, and implications for understanding more complex processes, like carcinogenesis are suggested. Studies of recent years provided evidence that regulation of DNA replication in bacteria is more complex than previously anticipated. Multiple layers of control seem to ensure coordination of this process with the increase of cellular mass and the division cycle. Metabolic processes and membrane composition may serve as points where integration of genome replication with growth conditions occurs. It is also likely that coupling of DNA synthesis with cellular metabolism may involve interactions of replication proteins with other macromolecular complexes, responsible for various cellular processes. Thus, the exact set of factors participating in triggering the replication initiation may differ depending on growth conditions. Therefore, understanding the regulation of DNA duplication requires placing this process in the context of the current knowledge on bacterial metabolism, as well as cellular and chromosomal structure. Moreover, in both Escherichia coli and eukaryotic cells, replication initiator proteins were shown to play other roles in addition to driving the assembly of replication complexes, which constitutes another, yet not sufficiently understood, layer of coordinating DNA replication with the cell cycle.

Download or read book The Origin and Direction of Replication of the Chromosome of E Coli B r written by Martin Leon Pato and published by . This book was released on 1968 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Chromosomal Replication in Escherichia Coli written by Frank Pou-Fan Leu and published by . This book was released on 2002 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book DNA Replication written by Hisao Masai and published by Springer. This book was released on 2018-01-22 with total page 581 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews the latest trends and future directions of DNA replication research. The contents reflect upon the principles that have been established through the genetic and enzymatic studies of bacterial, viral, and cellular replication during the past decades. The book begins with a historical overview of the studies on eukaryotic DNA replication by Professor Thomas Kelly, a pioneer of the field. The following chapters include genome-wide studies of replication origins and initiation factor binding, as well as the timing of DNA replications, mechanisms of initiation, DNA chain elongation and termination of DNA replication, the structural basis of functions of protein complexes responsible for execution of DNA replication, cell cycle-dependent regulation of DNA replication, the nature of replication stress and cells’ strategy to deal with the stress, and finally how all these phenomena are interconnected to genome instability and development of various diseases. By reviewing the existing concepts ranging from the old principles to the newest ideas, the book gives readers an opportunity to learn how the classical replication principles are now being modified and new concepts are being generated to explain how genome DNA replication is achieved with such high adaptability and plasticity. With the development of new methods including cryoelectron microscopy analyses of huge protein complexes, single molecular analyses of initiation and elongation of DNA replication, and total reconstitution of eukaryotic DNA replication with purified factors, the field is enjoying one of its most exciting moments, and this highly timely book conveys that excitement to all interested readers.

Download or read book Escherichia Coli Rec a Protein written by Peter J. McInerney and published by . This book was released on 1996 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Biology of the Gene written by James D. Watson and published by Benjamin-Cummings Publishing Company. This book was released on 2014 with total page 872 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now completely up-to-date with the latest research advances, the Seventh Edition retains the distinctive character of earlier editions. Twenty-two concise chapters, co-authored by six highly distinguished biologists, provide current, authoritative coverage of an exciting, fast-changing discipline.