Download or read book Differential Display written by Ron A. Leslie and published by OUP Oxford. This book was released on 2000-05-04 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the most challenging tasks facing the modern biological research laboratory is to make sense of the enormous amount of data being generated by various genome projects currently underway, and especially the human genome project. Understanding the ways in which genes are differentially expressed in various tissues and cell types, throughout ontogenetic development and in pathological processes, will go a long way towards understanding the function of all these 'new' genes and their protein products. Differential Display explains in detail how to perform the technique of RT-PCR Differential Display in various kinds of experimental biological systems. It also examines this technique in the context of other methods of studying differential gene expression such as subtractive hybridisation and the use of high-density gene microarrays combined with hybridisation techniques and automatic image analysis.
Download or read book Differential Display Methods and Protocols written by Peng Liang and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 321 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first edition of this book dedicated to differential display (DD) technology was published in 1997, we have witnessed an explosive interest in studying differential gene expression. The gene-hunting euphoria was initially powered by the invention of DD, which was gradually overtaken by DNA microarray technology in recent years. Then why is there still the need for second edition of this DD book? First of all, DD still enjoys a substantial lead over DNA microarrays in the ISI citation data (see Table 1), despite the h- dreds of millions of dollars spent each year on arrays. This may come as a surprise to many, but to us it implies that many of the DNA microarray studies went unpublished owing to their unfulfilled promises (1). Second, unlike DNA microarrays, DD is an “open”-ended gene discovery method that does not depend on prior genome sequence information of the organism being studied. As such, DD is applicable to the study of all living organisms—from bacteria, fungi, insects, fish, plants, to mammals—even when their genomes are not sequenced. Second, DD is more accessible technically and financially to most cost-conscious “cottage-industry” academic laboratories. So clearly DD still has its unique place in the modern molecular biological toolbox for gene expression analysis.
Download or read book Differential Display Reverse Transcription PCR DDRT PCR written by Sergio Colonna-Romano and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 135 pages. Available in PDF, EPUB and Kindle. Book excerpt: Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA. Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.
Download or read book Differential Display written by Ron A. Leslie and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Differential Display offers practical advice to researchers interested in using the technique of RT-PCR differential display to help them understand the function of individual genes in specific tissues or cells of an organism.
Download or read book Differential Display Method and Application written by Peng Liang and published by . This book was released on 1998 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book RNA Methodologies written by Robert E. Farrell Jr. and published by Elsevier. This book was released on 2010-07-22 with total page 794 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5’ and 3’ RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Use of Differential Display to Characterise Changes in Neutrphil Gene Expression in Response to Activation by FMLP written by Ke Lin and published by . This book was released on 1997 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Expression Genetics written by Arthur Beck Pardee and published by Eaton Publishing Company/Biotechniques Books. This book was released on 1999 with total page 636 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Use of Labeled Primers for Differential Display written by and published by . This book was released on 1995 with total page 19 pages. Available in PDF, EPUB and Kindle. Book excerpt: The differential display of eukaryotic cDNAs using PCR allows for determination of mRNA species differentially expressed when comparing two similar cell populations. This procedure uses a (T)12XY oligonucleotide as the 3 ft primer and an arbitrary 8-10-mer as the 5 ft primer. Labeling occurs by inclusion of [alpha][33P]-dATP in the PCR reaction. Two artifacts caused by this approach are (1) random printing from dT present from affinity purification of PolyA+RNA and (2) hybridization of the arbitrary primer to template target sequences on both cDNA strands. In this work, we have developed an approach for both eliminating smearing and identifying nonspecific bands on sequencing gels. By separately using 5 ft-end-labeled (T)12XY and arbitrary primers to label bands and comparing two differential display patterns rather than including labeled nucleotides in the PCR reaction itself, we can detect only those products incorporating the M12XY primer on the 3 ft ends and the arbitrary primer on 5 ft ends. Those bands that are generated randomly in the PCR reaction are readily detectable and can be ignored. If on the other hand, one is interested only in a diagnostic banding pattern for differential display, benefit can be derived from the simplicity of the pattern obtained when labeled (T)12XY is used.
Download or read book The Development and Application of a Fluorescent Differential Display Technique written by Graham John Speight and published by . This book was released on 2000 with total page 431 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Differentially Expressed Genes In Plants written by Axel Kornerup Hansen and published by CRC Press. This book was released on 2002-09-11 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis of changes in gene activity in tissues and cells of plants is a way of measuring developmental and environmental responses. This volume provides detailed accounts of new and established techniques used to carry out such analyses.
Download or read book Plant Molecular Biology A Laboratory Manual written by Melody S. Clark and published by Springer Science & Business Media. This book was released on 2013-11-27 with total page 551 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.
Download or read book Genome Mapping and Genomics in Fishes and Aquatic Animals written by Thomas D. Kocher and published by Springer Science & Business Media. This book was released on 2008-06-07 with total page 195 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mapping of animal genomes has generated huge databases and several new concepts and strategies, which are useful to elucidate origin, evolution and phylogeny. Genetic and physical maps of genomes further provide precise details on chromosomal location, function, expression and regulation of academically and economically important genes. The series Genome Mapping and Genomics in Animals provides comprehensive and up-to-date reviews on genomic research on a large variety of selected animal systems, contributed by leading scientists from around the world. This volume summarizes the first era of genomic studies of aquaculture species, in which the tools and resources necessary to support whole-genome sequencing were developed. These tools will enhance efforts toward selective breeding of aquaculture species. Included in this volume are summaries of work on salmonids, cyprinids, catfish, tilapias, European sea bass, Japanese flounder, shrimps and oysters.
Download or read book Differential Equation Solutions with MATLAB written by Dingyü Xue and published by Walter de Gruyter GmbH & Co KG. This book was released on 2020-04-06 with total page 451 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses the solutions of differential equations with MATLAB. Analytical solutions of differential equations are explored first, followed by the numerical solutions of different types of ordinary differential equations (ODEs), as well as the universal block diagram based schemes for ODEs. Boundary value ODEs, fractional-order ODEs and partial differential equations are also discussed.
Download or read book The Handbook of Plant Functional Genomics written by Guenter Kahl and published by John Wiley & Sons. This book was released on 2008-09-08 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this incisive, concise overview of this booming field, the editors -- two of the leading figures in the field with a proven track record -- combine their expertise to provide an invaluable reference on the topic. Following a treatment of transcriptome analysis, the book goes on to discuss replacement and mutation analysis, gene silencing and computational analysis. The whole is rounded off with a look at emerging technologies. Each chapter is accompanied by a concise overview, helping readers to quickly identify topics of interest, while important, carefully selected words and concepts are explained in a handy glossary. Equally accessible to both experienced scientists and newcomers to the field.
Download or read book Differential Display written by Colonna-Romano and published by . This book was released on 1998 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
