Download or read book Development of PCSA Targeted Minibodies for Imaging of Prostate Cancer written by and published by . This book was released on 2009 with total page 8 pages. Available in PDF, EPUB and Kindle. Book excerpt: The humanized antibody to prostate stem cell antigen (PSCA), a cell surface marker on prostate cancer cells, combined with PET scanning was developed to image prostate tumor. The antibody was successful in preclinical model, although humanization caused a loss of affinity relative to the parental monoclonal antibody. To improve the imaging agent, multiple versions of humanized PSCA antibody fragments were created and subsequently tested for their abilities to image in vivo using micro-PET, with the goal to eventually detect distant metastases. Both the engineered minibodies and diabodies were able to successfully detect tumor, and the affinity that was decreased upon humanization was improved. Experiments are ongoing to characterize the affinity matured variant of the minibodies, therefore a lead agent has not been chosen, but this is expected to happen in the next year. On the other hand, a human PSCA knockin mouse model was generated, verified and maintained. This genetically engineered murine line is being expanded and will be used to cross to a known model of mouse prostate cancer in the next year.
Download or read book Anti PSCA Minibody for ImmunoPET Imaging of Prostate Cancer in Preclinical Models written by Scott Matthew Knowles and published by . This book was released on 2013 with total page 171 pages. Available in PDF, EPUB and Kindle. Book excerpt: Prostate stem cell antigen (PSCA) is a cell surface glycoprotein that has low levels of expression in normal prostate, bladder, and stomach, but is expressed in 83-100%, and overexpressed in 40-100%, of prostate cancers. PSCA expression has been shown to correlate with the Gleason score, seminal vesicle and capsular invasion, advanced clinical stage, androgen independence, MYC gene-amplification, and a poor prognosis. PSCA is also highly overexpressed in the majority of prostate cancer bone metastases (87- 100%) and many metastases to other sites (67% liver, 67-95% lymph node). ImmunoPET is a highly flexible imaging technique that can be utilized to image virtually any cell surface protein using radiolabeled antibodies or antibody fragments. This dissertation describes the use of a radiolabeled anti-PSCA A11 minibody (scFv-CH3 dimer, 80 kDa) for immunoPET imaging of PSCA expression in mouse models of prostate cancer and demonstrates the potential of the A11 minibody for highly specific imaging of local prostate cancer and metastases in the clinic. In this work, 124I and 89Zr-DFO radiolabeling of the anti-PSCA minibody are compared. Imaging with both radionuclides showed specific tumor targeting in 22rv1×PSCA and LAPC-9 tumors and achieved high contrast imaging in both tumor models. However, 124I-labeled A11 minibody achieved superior tumor:soft tissue contrast and was determined to have higher clinical potential. A quantitative method of & mu;PET imaging of both 124I and 89Zr radionuclides using recovery coefficient based partial volume correction was also established and validated. The PSCA promoter contains an androgen response element and expression of PSCA has been shown to be regulated by androgens in the mouse prostate and in human prostate cancer biopsy tissue. In the present work, treatment with the second generation anti-androgen MDV-3100 was demonstrated to cause downregulation of PSCA in LAPC-9 xenografts. In addition, 124I-labeled A11 minibody showed decreased uptake in LAPC-9 xenografts treated with MDV-3100 compared to vehicle controls suggesting a potential use for the A11 minibody in imaging response to androgen deprivation therapy. The highest percentage of prostate cancer metastases are osteoblastic bone metastases and most clinical staging of prostate cancer metastases utilizes 99mTc-MDP or 18F- Fluoride bone scans to image increases in bone turnover in response to these metastases. In this work we demonstrate that 124I-labeled A11 minibody has higher sensitivity and specificity for detecting LAPC-9 intratibial xenografts than 18F-Fluoride bone scans. The A11 minibody binds only to human PSCA (hPSCA) and does not cross-react with the murine homologue mPSCA. In order to address the background uptake caused by endoge- nous hPSCA expression, the creation of an hPSCA knock-in mouse and imaging using the A11 minibody in this model is described. We found a pattern of hPSCA expression in these mice similar to the pattern of normal expression in humans. Uptake of 124I-labeled A11 minibody correlated strongly with the expression of hPSCA, but demonstrated that the overall increase in background uptake of the A11 minibody due to endogenous expression of human PSCA is minimal. The results presented in this work demonstrate robust targeting of PSCA expression by the A11 minibody and clinical investigation of the A11 minibody for imaging local prostate cancer and prostate cancer metastases is warranted.
Download or read book Development of PSMA targeted Imaging Agents for Prostate Cancer written by Tanushree Ganguly and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Our SPECT-based radioagent exhibited increasing uptake in the PSMA(+) LNCaP cells over time both in vitro and in vivo. More importantly, it was found that it was rapidly internalized into LNCaP cells, presumably through the PSMA enzyme-inhibitor complex. In addition, a convergent approach for generating the radio-agent was explored.
Download or read book Biomedical Image Processing written by Thomas Martin Deserno and published by Springer Science & Business Media. This book was released on 2011-03-01 with total page 617 pages. Available in PDF, EPUB and Kindle. Book excerpt: In modern medicine, imaging is the most effective tool for diagnostics, treatment planning and therapy. Almost all modalities have went to directly digital acquisition techniques and processing of this image data have become an important option for health care in future. This book is written by a team of internationally recognized experts from all over the world. It provides a brief but complete overview on medical image processing and analysis highlighting recent advances that have been made in academics. Color figures are used extensively to illustrate the methods and help the reader to understand the complex topics.
Download or read book Anatomy for Urologic Surgeons in the Digital Era written by Emre Huri and published by Springer Nature. This book was released on 2021-11-01 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a practical guide in the use of imaging and visualization technologies in urology. It details how output from diagnostic systems, can be represented through synthetic, virtual and augmented reality tools, such as holograms and three dimensional (3D) modelling and how they can improve everyday surgical procedures including laparoscopic, robotic-assisted, open, endoscopic along with the latest and most innovative approaches. Anatomy for Urologic Surgeons in the Digital Era: Scanning, Modelling and 3D Printing systematically reviews diagnostic imaging, visualization tools available in urology and is a valuable resource for all practicing and in-training urological surgeons.
Download or read book Targeted Radionuclide Therapy written by Tod W. Speer and published by Lippincott Williams & Wilkins. This book was released on 2012-03-28 with total page 564 pages. Available in PDF, EPUB and Kindle. Book excerpt: Radioimmunotherapy, also known as systemic targeted radiation therapy, uses antibodies, antibody fragments, or compounds as carriers to guide radiation to the targets. It is a topic rapidly increasing in importance and success in treatment of cancer patients. This book represents a comprehensive amalgamation of the radiation physics, chemistry, radiobiology, tumor models, and clinical data for targeted radionuclide therapy. It outlines the current challenges and provides a glimpse at future directions. With significant advances in cell biology and molecular engineering, many targeting constructs are now available that will safely deliver these highly cytotoxic radionuclides in a targeted fashion. A companion website includes the full text and an image bank.
Download or read book Biological Dosimetry written by W. G. Eisert and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: In October 1982, a small international symposium was held at the Gesellschaft fUr Strahlen- und Umweltforschung mbH (GSF) in Munich as a satellite meeting of the IX International Conference on Analytical Cytology. The symposium focussed on cytometric approaches to biological dosimetry, and was, to the best of our knowledge, the first meeting on this subject ever held. There was strong encouragement from the 75 attendees and from others to publish a proceedings of the symposium. Hence this book, containing 30 of the 36 presentations, has been assembled. Dosimetry, the accurate and systematic determination of doses, usually refers to grams of substance administered or rads of ionization or some such measure of exposure of a patient, a victim or an experimental system. The term also can be used to describe the quantity of an ultimate, active agent as delivered to the appropriate target material within a biological system. Thus, for mutagens, one can speak of DNA dosimetry, meaning the number of adducts produced in the DNA of target cells such as bone-mar row stem cells or spermatogonia.
Download or read book Mesothelioma written by Giovanni Luca Ceresoli and published by Springer. This book was released on 2019-06-19 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers an updated review of malignant mesothelioma, including the latest advances in our understanding of its genetic control and molecular biology, as well as pre-clinical and clinical research. It also presents state-of-the-art diagnostic approaches and therapeutic options, and an open discussion on the future prospects for patient management. Malignant mesothelioma is an enormous global health problem related to asbestos exposure. Despite the best efforts of scientists and oncologists, the prognosis for those affected remains poor. Due to anatomical characteristics and non-specific symptoms, the diagnosis of mesothelioma at an early stage is often difficult, while surgery and radiotherapy are only of limited use, even if some multimodality approaches seem promising. In turn, medical treatments are sometimes successful in tumor control, but have little impact on overall survival. However, advances in our understanding of the disease’s biology, together with the availability of new drugs and combinations, make mesothelioma an essential and highly topical field for pre-clinical and clinical studies. This book is subdivided into four parts: epidemiology and preclinical data, diagnosis, therapy, and extrathoracic mesothelioma. It highlights the progress made in a variety of areas – e.g. in vitro and in vivo experimental models, genetics, environment, biomarkers, targeting agents, immunotherapy, metabolic imaging and ongoing clinical trials – and describes the standard clinical management of mesothelioma patients, including those with extra-thoracic localizations. Given its scope, the book offers an invaluable tool for researchers, oncologists and clinicians alike.
Download or read book Cell and Molecular Biology of Breast Cancer written by Heide Schatten and published by Springer Science & Business Media. This book was released on 2013-09-27 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Highlighting recent advances in our understanding of breast cancer, this book is intended for a wide audience as a reference book. Included are reviews of genetics, epigenetics, various aspects of cell and molecular biology, and several other areas of breast cancer that are aimed at determining new intervention sites for treatments and cures of the disease. The chapters are written by internationally recognized experts and include reviews of key topics in breast cancer research. Each chapter highlights the new aspects of specific research topics and the various impacts of designing new strategies as well as identifies new targets for therapeutic intervention. The topics addressed are selected to be of interest to patients, scientists, students, teachers, and anyone else interested in expanding their knowledge of breast cancer imaging, diagnostics, therapeutics, or basic biomedical research on breast cancer.
Download or read book Epigenetics and Metabolomics written by Paban K. Agrawala and published by Elsevier. This book was released on 2021-08-25 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt: Epigenetics and Metabolomics, a new volume in the Translational Epigenetics series, offers a synthesized discussion of epigenetic control of metabolic activity, and systems-based approaches for better understanding these mechanisms. Over a dozen chapter authors provide an overview of epigenetics in translational medicine and metabolomics techniques, followed by analyses of epigenetic and metabolomic linkage mechanisms likely to result in effective identification of disease biomarkers, as well as new therapies targeting the removal of the inappropriate epigenetic alterations. Epigenetic interventions in cancer, brain damage, and neuroendocrine disease, among other disorders, are discussed in-depth, with an emphasis on exploring next steps for clinical translation and personalized healthcare. Offers a synthesized discussion of epigenetic regulation of metabolic activity and systems-based approaches to power new research Discusses epigenetic control of metabolic pathways and possible therapeutic targets for cancer, neurodegenerative, and neuroendocrine diseases, among others Provides guidance in epigenomics and metabolomic research methodology
Download or read book Genetics of Human Cancer written by Joseph F. Fraumeni and published by . This book was released on 1977 with total page 541 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Urologic Surgery in the Digital Era written by Domenico Veneziano and published by Springer Nature. This book was released on 2021-11-09 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a practical guide to the use of the latest applicable surgical procedures and present novel pathways. It details how to utilize a range of techniques including three-dimensional (3D) laparoscopy and those that require robotic assistance. Guidance is also contained on innovative tools including confocal laser endomicroscopy along with the application of biomaterials, artificial intelligence and other exponential technologies, enabling the reader to systematically develop a thorough understanding of these methodologies. Urologic Surgery in the Digital Era: Next Generation Surgery and Novel Pathways systematically reviews the application of the latest surgical techniques and potential future developments in urology. Therefore, it is a critical resource for all practicing and trainee urological surgeons.
Download or read book Phosphoinositides I Enzymes of Synthesis and Degradation written by Tamas Balla and published by Springer Science & Business Media. This book was released on 2012-03-14 with total page 361 pages. Available in PDF, EPUB and Kindle. Book excerpt: Phosphoinositides play a major role in cellular signaling and membrane organization. During the last three decades we have learned that enzymes turning over phosphoinositides control vital physiological processes and are involved in the initiation and progression of cancer, inflammation, neurodegenerative, cardiovascular, metabolic disease and more. In two volumes, this book elucidates the crucial mechanisms that control the dynamics of phosphoinositide conversion. Starting out from phosphatidylinositol, a chain of lipid kinases collaborates to generate the oncogenic lipid phosphatidylinositol(3,4,5)-trisphosphate. For every phosphate group added, there are specific lipid kinases – and phosphatases to remove it. Additionally, phospholipases can cleave off the inositol head group and generate poly-phosphoinositols, which act as soluble signals in the cytosol. Volume I untangles the web of these enzymes and their products, and relates them to function in health and disease. Phosphoinositide 3-kinases and 3-phosphatases have received a special focus in volume I, and recent therapeutic developments in human disease are presented along with a historical perspective illustrating the impressive progress in the field.
Download or read book Molecular Oncology written by Frank Joseph Rauscher (III) and published by Cambridge University Press. This book was released on 2014 with total page 985 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reviews the origins of molecular oncology, including technologies for cancer analysis, key pathways in human malignancies, and available pharmacologic therapies.
Download or read book Artificial Intelligence Methods Applied to Urban Remote Sensing and GIS written by Chang-Wook Lee and published by Mdpi AG. This book was released on 2021-11-11 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is based on Special Issue "Artificial Intelligence Methods Applied to Urban Remote Sensing and GIS" from early 2020 to 2021. This book includes seven papers related to the application of artificial intelligence, machine learning and deep learning algorithms using remote sensing and GIS techniques in urban areas.
Download or read book Ovarian Cancer Biomarkers written by Khalid El Bairi and published by Springer Nature. This book was released on 2021-10-09 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book comprehensively summarizes the biology, etiology, and pathology of ovarian cancer and explores the role of deep molecular and cellular profiling in the advancement of precision medicine. The initial chapter discusses our current understanding of the origin, development, progression and tumorigenesis of ovarian cancer. In turn, the book highlights the development of resistance, disease occurrence, and poor prognosis that are the hallmarks of ovarian cancer. The book then reviews the role of deep molecular and cellular profiling to overcome challenges that are associated with the treatment of ovarian cancer. It explores the use of genome-wide association analysis to identify genetic variants for the evaluation of ovarian carcinoma risk and prognostic prediction. Lastly, it highlights various diagnostic and prognostic ovarian cancer biomarkers for the development of molecular-targeted therapy.
Download or read book Sobotta Dissection Atlas written by Friedrich Paulsen and published by Elsevier Health Sciences. This book was released on 2017-08-21 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: Präparieren? Sobotta Präparieratlas! - Angepasst an die 24. Auflage Die Anatomie steht an, der Anatomie-Atlas und das Präparier-Besteck sind gekauft, das universitätseigene Präparierskript ist zur Hand - und ab gehts in den Präpariersaal. Aber halt - meinen teuren Atlas möchte ich nicht mitnehmen in den Präpariersaal, nur woher bekomme ich gute Abbildungen, die mir erklären, was ich am Körperspender wo genau sehe? Hier hilft der Sobotta Präparieratlas weiter! In diesem handlichen Atlas sind alle für das Präparieren wichtigen Sobotta-Abbildungen zusammengestellt, überaus realisitätsnah, besonders detailliert wo nötig und zu speziellen Themen um echte Leichenfotos ergänzt. Das Präparierskript der Uni liefert die Anleitungen, der Sobotta Präparieratlas die Abbildungen dazu. Damit der gute Atlas zu Hause bleiben kann! Bilinguale Ausgabe Deutsch-Englisch mit lateinischer Nomenklatur The dissection course is due? Then the new Dissection Atlas is a must-have! This convenient hands-on atlas compiles all essential anatomic images necessary for successful dissection. Spiral binding and firm, wipeable pages make the Dissection Atlas the ideal companion for the dissection lab – combinable with all other atlases or lecture notes. Particularly detailed and realistic images make it easy to clearly recognise anatomic structures and, therefore, to master the real situation in the dissection lab. Ideally equipped for dissection class: Step by step:All body areas are bundled by chapter following the order in your course Layer by layer:Successive images allow effortless understanding of every single step in the dissection process For those who already study with the Sobotta Atlas: The chapter division is consistent with that of our three-volume Sobotta Atlas of Human Anatomy which facilitates consulting your books for reinforced learning. In addition, the original image numbers from the Sobotta Atlas are provided with each image – retrieval guaranteed! Bilingual Edition German - English with Latin Nomenclature
