Download or read book Development of New Spectrophotometric and HPLC Methods written by Madduri Ramachandra Reddy and published by LAP Lambert Academic Publishing. This book was released on 2014-09-09 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical chemistry is playing a vital role in determination of various drugs and pharmaceutical products.The chemical features of selected drug molecules still offer a lot of scope for the development of new UV and visible spectrophotometric and HPCL methods hopefully with better sensitivity, selectiviy, precision and accuracy.The author has made some attempts in this direction and succeeded in developing some new methods having advantages of one or more of the desirable features.

Download or read book HPLC Method Development for Pharmaceuticals written by Satinder Ahuja and published by Elsevier. This book was released on 2011-09-21 with total page 533 pages. Available in PDF, EPUB and Kindle. Book excerpt: High pressure, or high performance, liquid chromatography (HPLC) is the method of choice for checking purity of new drug candidates, monitoring changes during scale up or revision of synthetic procedures, evaluating new formulations, and running control/assurance of the final drug product. HPLC Method Development for Pharmaceuticals provides an extensive overview of modern HPLC method development that addresses these unique concerns. Includes a review and update of the current state of the art and science of HPLC, including theory, modes of HPLC, column chemistry, retention mechanisms, chiral separations, modern instrumentation (including ultrahigh-pressure systems), and sample preparation. Emphasis has been placed on implementation in a pharmaceutical setting and on providing a practical perspective. HPLC Method Development for Pharmaceuticals is intended to be particularly useful for both novice and experienced HPLC method development chemists in the pharmaceutical industry and for managers who are seeking to update their knowledge. Covers the requirements for HPLC in a pharmaceutical setting including strategies for software and hardware validation to allow for use in a regulated laboratory Provides an overview of the pharmaceutical development process (clinical phases, chemical and pharmaceutical development activities) Discusses how HPLC is used in each phase of pharmaceutical development and how methods are developed to support activities in each phase

Download or read book Development and Publication of New Spectrophotometric Methods of Analysis written by G.F. Kirkbright and published by . This book was released on 1965 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Development and Validation of HPLC Method for Combined Dosage Form written by Digbijay Kumar and published by LAP Lambert Academic Publishing. This book was released on 2013 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pharmaceutical products formulated with more than one drug, typically referred to as combination products, are intended to meet previously unmet patients need by combining the therapeutic effects of two or more drugs in one product. These combination products can present daunting challenges to the analytical chemist responsible for the development and validation of analytical methods. This presentation will discuss the development and validation of analytical method Spectrophotometric and High performance liquid chromatography (HPLC), for drug products containing more than one active ingredient. This book deals with various approaches applied for the development and validation of analytical method for paracetamol and pamabrom.

Download or read book Ultra Performance Liquid Chromatography Mass Spectrometry written by Mu Naushad and published by CRC Press. This book was released on 2014-03-18 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a unique collection of up-to-date UPLC-MS/MS (ultra performance liquid chromatography-tandem mass spectrometric) methods for the separation and quantitative determination of pesticides, capsaicinoids, heterocyclic amines, aflatoxin, perfluorochemicals, acrylamide, procyanidins and alkaloids, lactose content, phenolic compounds, vitamins, and aroma and flavor compounds in a wide variety of foods and food products. With contributions by experts in interdisciplinary fields, this reference offers practical information for readers in research and development, production, and routing analysis of foods and food products.

Download or read book Novel Spectrophotometric and HPLC Method Development and Validation written by Pratik Mehta and published by LAP Lambert Academic Publishing. This book was released on 2013-01 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt: Drug manufacturing control requires high level and intensive analytical and chemical support of all stages to ensure the drug's quality and safety. Highly specific and sensitive analytical techniques hold the key to the design, development, standardization and quality control of medicinal products. The book is focussed on development and validation of RP-HPLC method for simultaneous estimation of bromhexine HCl, dextromethorphan HBr, and guaiphenesin, colorimetric method for estimation of ferrous fumarate and zinc sulphate, and UV spectrophotometric method for simultaneous estimation of ambroxol HCl, cetirizine HCl, and dextromethorphan HBr, form their combined dosage form. The analysis should be useful to some pharmaceutical companies, students of pharmacy field or anyone else seeking knowledge in the respective field.

Download or read book Analytical Method Development and Validation written by Michael E. Swartz and published by CRC Press. This book was released on 2018-10-03 with total page 95 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes analytical methods development, optimization and validation, and provides examples of successful methods development and validation in high-performance liquid chromatography (HPLC) areas. The text presents an overview of Food and Drug Administration (FDA)/International Conference on Harmonization (ICH) regulatory guidelines, compliance with validation requirements for regulatory agencies, and methods validation criteria stipulated by the US Pharmacopia, FDA and ICH.

Download or read book Validated Uv Spectrophotometric and Rp Hplc Method for Two Drugs written by Ankur Kothari and published by LAP Lambert Academic Publishing. This book was released on 2012-08 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt: The proposed method was quite simple and do not require any pretreatment of drugs and tedious extraction procedure. The method has wider linear range. Hence, the data presented in the manuscript "Validated UV Spectrophotometric and RP-HPLC method development for the simultaneous estimation of Sitagliptin and Simvastatin in marketed Formulation" demonstrate that the proposed method is linear and offer advantages of reagent availability and stability, less time consumption.The statisticalanalysis proves that the methods are reproducible and selective for the estimation of Sitagliptin and Simvastatin in marketed tablet formulation. Thus it can be extended for routine analysis of Sitagliptin and Simvastatin in pharmaceutical industries, hospitals, and research laboratories. These all process is done for the betterment of medicine, so that no or less side effects occur.

Download or read book The Second SeaWiFS HPLC Analysis Round Robin Experiment SeaHARRE 2 written by National Aeronautics and Space Administration (NASA) and published by Createspace Independent Publishing Platform. This book was released on 2018-06-15 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt: Eight international laboratories specializing in the determination of marine pigment concentrations using high performance liquid chromatography (HPLC) were intercompared using in situ samples and a variety of laboratory standards. The field samples were collected primarily from eutrophic waters, although mesotrophic waters were also sampled to create a dynamic range in chlorophyll concentration spanning approximately two orders of magnitude (0.3 25.8 mg m-3). The intercomparisons were used to establish the following: a) the uncertainties in quantitating individual pigments and higher-order variables (sums, ratios, and indices); b) an evaluation of spectrophotometric versus HPLC uncertainties in the determination of total chlorophyll a; and c) the reduction in uncertainties as a result of applying quality assurance (QA) procedures associated with extraction, separation, injection, degradation, detection, calibration, and reporting (particularly limits of detection and quantitation). In addition, the remote sensing requirements for the in situ determination of total chlorophyll a were investigated to determine whether or not the average uncertainty for this measurement is being satisfied. The culmination of the activity was a validation of the round-robin methodology plus the development of the requirements for validating an individual HPLC method. The validation process includes the measurements required to initially demonstrate a pigment is validated, and the measurements that must be made during sample analysis to confirm a method remains validated. The so-called performance-based metrics developed here describe a set of thresholds for a variety of easily-measured parameters with a corresponding set of performance categories. The aggregate set of performance parameters and categories establish a) the overall performance capability of the method, and b) whether or not the capability is consistent with the required accuracy objectives. Goddard Space Flight Center

Download or read book Development of New Liquid Chromatography Techniques for Mass Spectrometric Analysis of Metabolites and Proteins written by Zhihui Wen and published by . This book was released on 2006 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Mass Spectrometry in Drug Discovery written by David T. Rossi and published by CRC Press. This book was released on 2001-11-07 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass Spectrometry in Drug Discovery summarizes the theory, instrumentation, techniques, and application of mass spectrometry and atmospheric pressure ionization to screening, evaluating, and improving the performance and quality of drug candidates. It provides time- and cost-efficient approaches for the generation and analysis of effective pharmaceuticals, covers advances in combinatorial chemistry, molecular biology, bioanalysis automation, and computing, and demonstrates the use of mass spectrometry in the assessment of disease states, drug targets, and potential drug agents.

Download or read book Handbook of Advanced Chromatography Mass Spectrometry Techniques written by Michal Holcapek and published by Elsevier. This book was released on 2017-09-07 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of Advanced Chromatography /Mass Spectrometry Techniques is a compendium of new and advanced analytical techniques that have been developed in recent years for analysis of all types of molecules in a variety of complex matrices, from foods to fuel to pharmaceuticals and more. Focusing on areas that are becoming widely used or growing rapidly, this is a comprehensive volume that describes both theoretical and practical aspects of advanced methods for analysis. Written by authors who have published the foundational works in the field, the chapters have an emphasis on lipids, but reach a broader audience by including advanced analytical techniques applied to a variety of fields. Handbook of Advanced Chromatography / Mass Spectrometry Techniques is the ideal reference for those just entering the analytical fields covered, but also for those experienced analysts who want a combination of an overview of the techniques plus specific and pragmatic details not often covered in journal reports. The authors provide, in one source, a synthesis of knowledge that is scattered across a multitude of literature articles. The combination of pragmatic hints and tips with theoretical concepts and demonstrated applications provides both breadth and depth to produce a valuable and enduring reference manual. It is well suited for advanced analytical instrumentation students as well as for analysts seeking additional knowledge or a deeper understanding of familiar techniques. Includes UHPLC, HILIC, nano-liquid chromatographic separations, two-dimensional LC-MS (LCxLC), multiple parallel MS, 2D-GC (GCxGC) methodologies for lipids analysis, and more Contains both practical and theoretical knowledge, providing core understanding for implementing modern chromatographic and mass spectrometric techniques Presents chapters on the most popular and fastest-growing new techniques being implemented in diverse areas of research

Download or read book Analytical Method Development and Validation by Uv and Hplc Techniques written by Hajera Khan and published by LAP Lambert Academic Publishing. This book was released on 2012 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gemifloxacin, a flouroquinoline derivative has antibacterial activity. Ambroxol dibromoaminobenzyl derivatives have mucolytic activity.GEM and AMB are available in tablet dosage form (G-cin A, Lupin)for mucolytic action. The present work dealt with simultaneous estimation of GEM and AMB from bulk and tablet formulation by different UV spectrophotometric, RPHPLC and Dissolution techniques. Five UV methods were developed which are accurate, precise, rapid and economical for the estimation of GEM and AMB in Tablet dosage form. The developed HPLC method was validated in terms of accuracy, repeatability, and precision. A good linear relationship was observed for GEM An attempt has been made to carry out the dissolution study of the marketed formulation by applying four established UV-Visible Spectrophotometric methods for estimation of % release of the drug (GEM & AMB

Download or read book The Second SeaWiFS HPLC Analysis Round robin Experiment SeaHARRE 2 written by and published by . This book was released on 2005 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book High performance Liquid Chromatography And Mass Spectrometry Of Porphyrins Chlorophylls And Bilins written by Chang-kee Lim and published by World Scientific. This book was released on 2009-07-17 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: The porphyrins, chlorophylls, bilins and related tetrapyrroles are vital for all living organisms. Natural and synthetic tetrapyrroles are used extensively in foods, cosmetics, biotechnology, pharmaceuticals, diagnostics and medicine. Methods for their separation and characterization therefore, have a very wide area of applications. Yet, there is a dearth of books dedicated to HPLC and HPLC/MS of tetrapyrroles. Lim addresses this problem admirably by providing practical HPLC and HPLC/MS protocols coupled with in-depth chromatographic and mass spectrometric reference data. These are invaluable in the analysis, identification and characterization of porphyrins, chlorophylls, bilins and other related compounds found in biological and clinical materials. HPLC method development and optimization for coupling to mass spectrometry are also described in rich detail. Sample preparation, and suggestions for avoiding procedural artifacts during extraction of clinical and biological samples are discussed. Clinical biochemists involved in biochemical diagnosis of human porphyrias will find this monograph assuredly helpful, as would analysts, biochemists and chemists involved in the separation, isolation and characterization of natural and synthetic tetrapyrroles. Undoubtedly, Lim has contributed a master-piece containing sufficient background material for beginners and up-to-date references for all researchers in the field.

Download or read book Colorimetric and Fluorimetric Analysis of Organic Compounds and Drugs written by Maurice Pesez and published by . This book was released on 1974 with total page 706 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Development of Liquid Chromatography mass Spectrometric Assays and Sample Preparation Methods for the Biological Sample Analysis written by Sujatha Chilakala and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The area of biosample analysis encompasses a very broad range of assays which support the clinical and nonclinical studies. Biosample analysis is used to provide a quantitative or qualitative measure of the active drug and/or its metabolite(s) in the biological matrix for the purpose of pharmacokinetics, toxicokinetics, bioequivalence, and exposure-response (pharmacokinetics /pharmacodynamics) studies. Due to the significance of pharmacological analysis, sensitive, reproducible and robust analytical methods are critically needed for pharmacological studies of the biosamples. A bioanalytical method mainly contains two components I) Sample preparation II) detection of the compound. Therefore, the main aims of this thesis are the development of quantitative and qualitative analytical methods for the target compounds using LC-MS(/MS) and development of accelerated sample preparation for high throughput sample analysis for DNA and proteins.In this dissertation, a brief review on the method rationale, workflow of the method development, sample preparation methods, instrumentations and analytical method validation, are discussed in Chapter 1. Also, research projects were discussed and the techniques used in the experiments for this thesis were reviewed. As so, chapter II and III were mainly focused on the accelerated sample preparation methods for the high throughput sample analysis of DNA and proteins respectively, where the sample preparation time was significantly reduced from hours to minutes, which are suitable for qualitative and quantitative analysis of DNA and proteins. In Chapter IV, a systematic study on the structural characterization of the model glycoprotein Human IgG was described. In chapter V successful development of LC-MS method was developed for the determination of Oxygen -18 isotope enrichment in the phosphate samples in the positional isotope exchange reactions to study the reversibility of certain enzymatic reactions was described. Successful development and validation of a new and sensitive analytical LC-MS/MS method for the determination and quantitation of incorporation rates of decitabine, an anti-cancer drug which can be applied to determine the sensitivity and responsiveness in patients treated with decitabine was described in Chapter VI.