Download or read book Development of Fluorescent and Luminescent Probes for Monitoring Biological Events written by Minhee Lee and published by . This book was released on 2010 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Fluorescent and Luminescent Probes for Biological Activity written by W. T. Mason and published by Elsevier. This book was released on 1999-04-16 with total page 697 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes. The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research. TOPICS COVERED: * Single and multiphoton confocal microscopy * Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies * Applications of new optical probes for imaging proteins in gels * Probes and detection technologies for imaging membrane potential in live cells * Use of optical probes to detect microorganisms * Raman and confocal raman microspectroscopy * Fluorescence lifetime imaging microscopy * Digital CCD cameras and their application in biological microscopy

Download or read book Fluorescent Energy Transfer Nucleic Acid Probes written by Vladimir V. Didenko and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. These probes signal detection of their targets by changing either the intensity or the color of their fluorescence. Not surpr- ingly, these luminous, multicolored probes carry more flashy names than their counterparts in the other fields of molecular biology. In recent years, fluor- cent probes and assays, which make use of energy transfer, have multiplied at a high rate and have found numerous applications. However, in spite of this explosive growth in the field, there are no manuals summarizing different p- tocols and fluorescent probe designs. In view of this, the main objective of Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is to provide such a collection. Oligonucleotides with one or several chromophore tags can form fluor- cent probes capable of energy transfer. Energy transport within the probe can occur via the resonance energy transfer mechanism, also called Förster tra- fer, or by non-Förster transfer mechanisms. Although the probes using Förster transfer were developed and used first, the later non-Förster-based probes, such as molecular beacons, now represent an attractive and widely used option. The term “fluorescent energy transfer probes” in the title of this book covers both Förster-based fluorescence resonance energy transfer (FRET) probes and probes using non-FRET mechanisms. Energy transfer probes serve as molecule-size sensors, changing their fluorescence upon detection of various DNA reactions.

Download or read book Optical Probes in Biology written by Jin Zhang and published by CRC Press. This book was released on 2016-04-19 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical probes, particularly the fluorescent varieties, enable researchers to observe cellular events in real time and with great spatial resolution. Optical Probes in Biology explores the diverse capabilities of these powerful and versatile tools and presents various approaches used to design, develop, and implement them. The book examines the use

Download or read book Fluorescence Microscopy and Fluorescent Probes written by Jan Slavík and published by Springer Science & Business Media. This book was released on 1998-08-31 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: The April 1997 conference held in Prague attracted the cream of primarily European and Russian researchers (with a handful from the US, primarily from the U. of Maryland School of Medicine) to the burgeoning biological and medical applications of innovative optical technology, particularly laser confocal fluorescence microscopy. Six invited review lectures illuminate these recent developments and their role in cell biology, including computer simulations of molecular behavior. The 34 other papers focus on the major themes of: new developments in fluorescence instrumentation; fluorescent probes; nucleic acid labels; and other fluorescent labels, markers, and fluorogenic substrates. Annotation copyrighted by Book News, Inc., Portland, OR

Download or read book Introduction to Fluorescence Sensing written by Alexander P. Demchenko and published by Springer Nature. This book was released on 2020-12-01 with total page 673 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides systematic knowledge of basic principles in the design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Fluorescence is the most popular technique in chemical and biological sensing because of its ultimate sensitivity, high temporal and spatial resolution and versatility that enables imaging within the living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response up to detection of single molecules. Its application areas range from control of industrial processes to environment monitoring and clinical diagnostics. Being a guide for students and young researchers, it also addresses professionals involved in active basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.

Download or read book Introduction to Fluorescence Sensing written by Alexander P. Demchenko and published by Springer Nature. This book was released on 2023-02-15 with total page 772 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence is the most popular technique in chemical and biological sensing because of its ultimate sensitivity, high temporal and spatial resolution and versatility that enables imaging within the living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response up to detection of single molecules. Its application areas range from control of industrial processes to environment monitoring and clinical diagnostics. This book provides systematic knowledge of basic principles in design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Being a guide for students and young researchers, it also addresses professionals involved in active basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.

Download or read book Development of Fluorescent Molecular Probes for Potential Biological and Materials Applications written by Maura Loew and published by . This book was released on 2014 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book DEVELOPMENT OF NEAR INFRARED FLUORESCENT PROBES FOR MONITORING LYSOSOMAL PH CHANGES written by and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract : Lysosome is an acidic membrane-bound organelle containing more than 70 hydrolytic enzymes that breaks down different biological macromolecules. Substantial lysosomal pH disruption can cause lysosome malfunction and consequently lead to lysosomal storage disease. Therefore, it is essential to precisely monitor lysosomal pH changes in order to explore cellular functions and get insightful understanding of physiological and pathological processes. Fluorescence imaging based on fluorescent probes is a powerful technique to monitor lysosomal pH changes because of advantageous features including operational simplicity, high sensitivity, non-invasive approach, and high spatial resolution. However, most of the reported lysosomal pH probes are based on Stokes-shift fluorescence with lower energy emission under higher energy excitation, and exhibit less than 600 nm absorption and emission wavelengths, which causes cellular and tissue photodamage and contains biological fluorescence background. In order to avoid these issues, we developed near-infrared fluorescent probes based on single-photon anti-Stokes fluorescence with near-infrared excitations and emissions. We significantly improved biocompatibility and water-solubility of fluorescent probes by introducing mannose residues to the fluorophores through oligo(ethylene glycol) tethered spacers for sensitive detection of lysosomal pH changes in two near-infrared channels. In order to take advantage of ratiometric and near-infrared imaging to overcome systematic errors of intensity-based fluorescent probes caused by probe concentration variation and uneven distribution, temperature, solvent polarity, and excitation light fluctuation, we developed ratiometric near-infrared fluorescent probes for ratiometric detection of lysosomal pH changes by introducing tetraphenylethene (TPE) dyes to hemicyanine dyes. Gradual lysosomal pH decreases result in gradual increases of hemicyanine fluorescence, and corresponding concomitant decreases of TPE fluorescence. The probes allow for development of various ratiometric near-infrared fluorescent probes for quantitative and comparative reliable analyses of cations, reactive nitrogen, oxygen and sulfur species by conjugating various biosensing groups into the near-infrared hemicyanine moieties.

Download or read book SMALL MOLECULE BASED FLUORESCENT MOLECULAR PROBES FOR FACILITATING BIOMEDICAL RESEARCH written by and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract : My thesis is focused on the development of fluorescent probes for biosensing and bioimaging within specific organelles. My main research efforts are mainly focused on the design, synthesis and biological applications of these new molecular probes. These new fluorescent probes I developed can be manipulated through the chemical modifications for binding to specific organelles capable of reporting localized bioinformation. Compared to the currently commercially available organelle-specific fluorescent stains, the advantages of the newly synthesized fluorescent probes include low cytotoxicity, high photostability, and long fluorescence lifetimes. These features are crucial for long-time tracking study of biological processes. Research on fluorescent probes with both analyte responsiveness and organelle targetability is a new and emerging area that has attracted increasing attention over the past few years. Because of their high sensitivity, specificity and fast response, these novel fluorescent probes have been proven to be useful tools for facilitating biomedical research. I have further extended the diversity by developing organelle-specific responsive probes capable of detecting changes in biomolecular levels and the microenvironment. My future research efforts give more considerations of the "low-concern" organelles, such as the Golgi apparatus, the endoplasmic reticulum, and ribosomes. Considering the tiny sizes of subcellular organelles, we anticipate that better visualization of the cellular events within specific organelles will rely on super-resolution optical microscopy with nanoscopic-scale resolution.

Download or read book Fluorescence Spectroscopy Imaging and Probes written by Ruud Kraayenhof and published by Springer. This book was released on 2012-01-30 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. This topic book, the second in his series, reflects this exciting scientific progress and deals, among others, with new approaches and new probes in fluorescence spectroscopy, single molecule fluorescence, applications in biomembrane and enzyme studies and imaging of living cells.

Download or read book Fluorescence Probes for Sensing written by Sheshanath V. Bhosale (Ed.) and published by . This book was released on 2018 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: The rapid progress in sensor science in recent years has resulted in the development of fluorescence probes with enhanced analytical capabilities. Because of the vast evolution of this research field, therefore, we have decided to combine all the research articles published in "Fluorescence Probes and Sensors" for a Special Issue (SI) book of Sensors which was focusing on the important role sensors play in "Fluorescent Probes and Sensors". Fluorescence novel Probes make an ideal candidate for promising applications in biological analytes and environmental monitoring. Fluorescent probes along with metal complexes have been developed as a new class of fluorophores with excellent properties. This book illustrating the suitability of newly developed sensors for fluorescent analysis applications, as well as describing novel applications of established sensors in solving real life analytical problems.

Download or read book Synthesis and Characterization of New Light Emitting Probes for Sensitive Detection of Bio molecules and Live Cells written by Shyamala Pravin Pillai and published by . This book was released on 2013 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: A variety of contemporary analytical platforms in technical and biological applications take advantage of labeling the objects of interest with fluorescent or luminescent tracers. Luminescent tracers take advantage of the unique property of some lanthanide metals to absorb and emit light. Long lifetime of lanthanide emission allows temporal gating of the signal, which avoids the short-lived background of interfering sample components. This property in combination with large Stokes shift contributes to extreme sensitivity of detection (ca. 10−13-10−14 M), which makes lanthanide-based probes suitable for large variety of challenging tasks ( e.g., intracellular detection of single DNA/RNA, or protein molecules, microbial pathogen detection in human specimens, tracing analysis, etc.). Luminescent probes include antenna fluorophore that absorbs light and transfers the excitation energy to a lanthanide metal tethered to antenna through chelation. The probe also contains crosslinking group that allows covalent labeling of the molecule of interest. Despite great potentials of lanthanide-based tracers the wide spread of the technology is impeded by very high price of commercially available probes to their complex structure. The goal is to develop novel approaches for the synthesis of lanthanide probes with improved quantum efficiency. This work discusses development of new strategies for synthesis of antena-fluorophores, development of new methods for introduction of the crosslinking groups in the luminescent probes and elucidates the mechanisms of chemical reactions leading to principal synthetic intermediates. New quinoline and quinolone based fluorescent compounds were synthesized, whose light emission can be conveniently tuned by simple structural modifications. Developed probes represent high-quantum yield, large Stokes shift fluorophores with amine-reactive and click-reactive groups convenient for conjugation. Some of these compounds can be used as sensitizers for lanthanide emission in design of highly sensitive luminescent probes. Obtained probes demonstrate efficient derivatization reactions allowing introduction of amine- or click-reactive crosslinking groups into the fluorophores. The reactivity of synthesized compounds is confirmed in reaction with low molecular weight nucleophiles as well as with click-reactive DNA-oligonucleotide counterparts. These reactive derivatives can be used for covalent attachment of the fluorophores to various biomolecules of interest including nucleic acids, proteins, live cells and small cellular metabolites. Synthesized compounds were characterized using N MR, steady-state and time-resolved fluorescence spectroscopy, as well UV absorption spectroscopy. This work also discusses the development of fluorescent derivatives of the antifungal drugs, posaconazole and caspofungin, for diagnostic imaging of fungal cells. Invasive fungal infections (IFI's) are a growing threat to human health particularly, with increase in the number of immune-compromised patient population, such as organ transplant recipients, al logeneic B MT, hematologic cancers; AIDS etc. The diagnosis of fungal infections is a complicated task and requires a combination of clinical observations, laboratory investigation, and radiological or other diagnostic imaging methods. Only 25% of I FI cases are diagnosed pre-mortem due to the current diagnostic challenges, which justifies the development of express diagnostic procedures. To address the issue fluorescent derivatives of the antifungal drugs, posaconazole and caspofungin, were synthesized. The fluorescent derivatives retained strong and highly specific binding to their cellular targets rendering the cells fluorescent. This new affinity-based approach strongly facilitates the detection of fungal pathogen thereby overcoming the current diagnostic challenges and can be used for clinical diagnostics. The power of this approach is not limited to fungal pathogens, but in fact represents a broader platform useful for detection of other classes of infections, both in the biological specimens and in the whole body using contemporary 3-D imaging approaches like fluorescence based in vivo imaging, luminescence imaging, positron emission tomography approach, and computer-assisted X-ray tomography.

Download or read book Dual locked Fluorescent Probes for Monitoring Biological Redox State written by Luling Wu and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Handbook of Fluorescence Spectroscopy and Imaging written by Markus Sauer and published by John Wiley & Sons. This book was released on 2010-12-23 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.

Download or read book Development and Applications of Novel Fluorescent Molecular Probe Strategies written by Dolonchampa Maji and published by . This book was released on 2017 with total page 149 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical imaging and spectroscopy technologies offer the ability to provide structural and functional information in a fast, low-cost, ionizing radiation free, highly sensitive and high throughput fashion. The diverse contrast mechanisms and complementary imaging platforms form the foundation for the application of optical imaging in pre-clinical studies of pathophysiological development as well as direct clinical application as a tool for diagnosis and therapy. Fluorescence imaging techniques have been one of the most rapidly adopted methods in biology and biomedicine. Visualization of biological processes and pathologic conditions at the cellular and tissue levels largely relies on the use of exogenous fluorophores or their bioconjugates. Some fluorescent molecular probes provide usable contrast for disease diagnosis due to their responsiveness to interactions with other molecular species and/or immediate microenvironment. As a result, understanding exogenous fluorescent contrast mechanisms will allow the development of efficient strategies for biomedical fluorescence imaging. The present work focuses on exploring novel fluorescent molecular probe strategies for imaging cancer and cardiovascular diseases. We have developed a platform for synthesizing activatable fluorescent molecular probes using the fluorescence quenching properties of copper (II) ions. We used these activatable probes for rapid imaging of cancerous tissue in vivo in mice. While developing these molecular probes, we discovered an unexpected molecular interaction that yields stable dimeric molecules. This finding can potentially enable the development of new molecular entities for modifying the signaling properties of fluorescent dyes to minimize background fluorescence. Although planar fluorescence imaging methods using exogenous molecular probes provide rapid information about molecular processes in vivo, extraction of depth information require complex data acquisition and image analysis methods. By designing a dual emission fluorescent probe incorporating two spectrally different fluorophore systems, we developed a method to successfully estimate the depth of fluorescent inclusions from planar imaging data and demonstrated the potential of using this approach to locate a blood vessel and tumorous tissue in mouse in vivo. An important feature of fluorescence methods is the availability of various techniques that provide complementary information. Combining the fluorescence intensity and lifetime properties of a biologically targeted near infrared fluorescent probe, we demonstrate an effective way to distinguish specific from nonspecific uptake mechanisms in cancer cells, an approach that can be translated in vivo. Alternatively, dynamic fluorescence imaging technique expands the scope of applications to include detection and estimation of the size of circulating cancer cells and clusters. The approach developed in this work could allow longitudinal monitoring of these cells, which are implicated in cancer metastases. To circumvent the shallow penetration of light using optical methods, we developed multimodal imaging approaches by incorporating a radionuclide for nuclear imaging into a broad spectrum near infrared fluorescent tumor targeting agent. This molecular construct allows for noninvasive whole body nuclear imaging of tumors, followed by fluorescence image guided resection. In each of these areas, novel fluorescent molecular probes were developed, characterized and applied to solve critical biomedical problems.

Download or read book Metal Enhanced Fluorescence written by Chris D. Geddes and published by John Wiley & Sons. This book was released on 2010-06-22 with total page 655 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence.