Download or read book Development of Boronic Acid based Spectroscopic Sensors for Saccharides written by Weijuan Ni and published by . This book was released on 2003 with total page 191 pages. Available in PDF, EPUB and Kindle. Book excerpt: Keywords: Boronic Acid, Sensor, Saccharides.
Download or read book Development of Boronic Acid Based Spectroscopic Sensors for Saccharides written by and published by . This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Boronic acid functional group is used widely as a recognition moiety for the development of sensors for saccharides, due to its unique strong and reversible interaction with diols. The fluorescence and/or UV spectroscopic method are the easiest and most common methods to signal the binding event of these receptors (boronic acid compounds) with saccharides. This dissertation consists of three major parts. First, a series of fluorescent diboronic acid compounds with an anthracene PET (photoinduced electron transfer) system were designed and synthesized (Chapter 1). These compounds can be used as sensors for cell surface carbohydrates, such as sialyl Lewis X (sLex), which are known to be biomarkers for certain cancers. Among these diboronic acid compounds, the lead compound was found to fluorescently label cells expressing sLex selectively. The structure of the lead compound was modified and new conformationally constrained analogs were synthesized. Second, the mechanism of the fluorescent photoinduced electron transfer (PET) system, which was used in our design of fluorescent sensors for sLex, was examined in detail using both density functional calculation (DFT) and model compound fluorescence studies. Based on the study, a new hydrolysis mechanism was proposed (Chapter 2 and 3). Third, novel nitrophenol-based boronic acid reporter compounds, which show significant UV spectroscopic changes upon addition of sugars at neutral pH in aqueous solution, were designed and synthesized (Chapter 4). The design took advantage of the ability of a boronic acid functional group to modulate the pKa and/or the electron density of a neighboring group. These reporter compounds can be used as the recognition and signaling unit for the construction of polyboronic acid sensors for selective and specific recognitions of saccharides of biological significance.
Download or read book Carbohydrate Recognition written by Binghe Wang and published by John Wiley & Sons. This book was released on 2011-09-06 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contains contributions from interdisciplinary scientists to collectively address the issue of targeting carbohydrate recognition for the development of novel therapeutic and diagnostic agents. The book covers (1) biological problems involving carbohydrate recognition, (2) structural factors mediating carbohydrate recognition, (3) design and synthesis of lectin mimics that recognize carbohydrate ligands with high specificity and affinity, and (4) modulation of biological and pathological processes through carbohydrate recognition.
Download or read book Glucose Sensing written by Chris D. Geddes and published by Springer Science & Business Media. This book was released on 2007-12-29 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential reference for any laboratory working in the analytical fluorescence glucose sensing field. The increasing importance of these techniques is typified in one emerging area by developing non-invasive and continuous approaches for physiological glucose monitoring. This volume incorporates analytical fluorescence-based glucose sensing reviews, specialized enough to be attractive to professional researchers, yet appealing to a wider audience of scientists in related disciplines of fluorescence.
Download or read book Boronic Acids in Saccharide Recognition written by Tony D. James and published by Royal Society of Chemistry. This book was released on 2006 with total page 185 pages. Available in PDF, EPUB and Kindle. Book excerpt: The desire to quantify the presence of analytes within diverse physiological, environmental and industrial systems has led to the development of many novel detection methods. In this arena, saccharide analysis has exploited the pair-wise interaction between boronic acids and saccharides. Boronic Acids in Saccharide Recognition provides a comprehensive review and critical analysis of the current developments in this field. It also assesses the potential of this innovative approach, outlining future lines of research and possible applications. Topics include: the molecular recognition of saccharides, the complexation of boronic acids with saccharides, fluorescent sensors and the modular construct of fluorescent sensors, further sensory systems for saccharide recognition and an extensive bibliography. This high level book is ideal for researchers both academic and industrial who require a comprehensive overview of the subject.
Download or read book Design and Synthesis of a Boronic Acid Sensor to Study Carbohydrate Binding Using SERS written by Paul Russell Petersen and published by . This book was released on 2010 with total page 67 pages. Available in PDF, EPUB and Kindle. Book excerpt: Carbohydrates are known to play a large number of significant roles in various biological and pathological processes such as cancer metastasis and cellular communication. This is because of their ability to bind a wide range of biological receptors such as proteins and viruses. Due to these important interactions, carbohydrate sensing has long been a main focus of research. These research strategies have included the use of aptamers, non-covalent interactions, and boronic acid-based receptors. Boronic acid-based sensors are of particular interest due to their selectivity for 1,2- or 1,3-diols. Within these boronic acid-based studies, a large variety of techniques were employed for detection including different fluorescent, electrochemical, polymeric, and colorimetric studies, as well as various surface bound sensors. One type of technique that has rarely been applied is Surface Enhanced Raman Spectroscopy or SERS. This strategy would be beneficial as it provides information about functional groups, which would aid in the identification of the bound sugar. In this thesis, we present work based on the development of a boronic acid-based carbohydrate receptor that will be used to study carbohydrate binding through SERS. The receptor design includes an aryl boronic acid for carbohydrate recognition, a nitrogen atom in close proximity to the boron center to enhance binding, and a terminal thiol for attachment to a metal surface for SERS. This sensor will be used to study the binding of different saccharides for sensing applications.
Download or read book New Supramolecular Approach for Sugar Analysis written by Serhan Boduroglu and published by . This book was released on 2006 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt: The main objective of the work presented in this thesis was to develop new methods for sugar sensing as well as sugar analysis. Boronic acids were chosen as sugar binding motif because of their affinity to sugars. For this purpose a novel type of boronic acid, 3-Pyridinylboronic acid (3pa), was developed. This boronic acid was identified as a key sensing element for reversible sugar complexation in an aqueous solution at physiological pH. In terms of sugar sensing, 3pba was successfully applied to develop a surface-enhanced Raman glucose sensor. In this study, indirect detection of glucose was demonstrated for the first time by using surface-enhanced Raman spectroscopy. Then, taking advantage of the high binding constant of 3pba with sugars, colorimetric detections of monosaccharides were done using absorption spectroscopy in the visible region. The mechanism of the high diol/triol binding of 3pba in a neutral pH was discussed based on the 1H, 13C, and 11B NMR spectroscopic studies. Monosaccharides as well as disaccharides were explored and K constants were verified by the NMR spectroscopy technique. In terms of sugar analysis, 3pba was used to develop an effective ionization agent for ESI-MS analysis of sugars. It was demonstrated that the dynamic covalent supramolecular binding, between the arylboronic acid and sugars allowed convenient in situ derivatization of the sugar analytes. The zwitterionic nature of 3pba allowed intense ion signals of both the corresponding cations and anions to be observed by a simple switching of the ESI-MS polarity. Isomeric monosaccharides, such as fructose, glucose, and galactose, and isomeric disaccharides, such as lactose and sucrose, were ionized and distinguished by a simple ESI-MS2 experiment. Moreover, zwitterionic aryl boronic acid allowed the facile formations of structurally well-defined gas phase ions with inert charge sites on trisaccharides. As a consequence, successful demonstration of sequencing of a trisaccharide was achieved.
Download or read book Design Synthesis and Optimization of an Optical Sensing System Utilizing Boronic Acid substituted Viologens and Pyrene based Fluorescent Dyes written by Soya Gamsey and published by . This book was released on 2006 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Interaction of Boronic Acid Based Self Ssembled Monolayers as a Potential Glucose Sensor written by and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Recognition of sugars such as glucose with phenylboronic acid- erminated self assembled monolayers (SAMs) was the basis for the designing and fabricating a biosensor. Self-assembled monolayers were formed on gold surfaces as shown by infrared spectroscopy. The continuous, repeatable increase of the electrochemical impedance as the concentration of glucose was increased gave indication of binding. This binding could be attributed to the precedent formation of relatively stable esters between phenylboronic acids and sugars. This impedance change upon binding between glucose and a phenylboronic acid-terminated SAM showed promise in the development of a glucose biosensor.
Download or read book Boronic Acids written by Dennis G. Hall and published by John Wiley & Sons. This book was released on 2006-05-12 with total page 571 pages. Available in PDF, EPUB and Kindle. Book excerpt: For the first time, the whole field of organoboronic acids is presented in one comprehensive handbook. Professor Dennis Hall, a rising star within the community, covers all aspects of this important substance class, including applications in chemistry, biology and medicine. Starting with an introduction to the structure, properties, and preparation of boronic acid derivatives, together with an overview of their reactions and applications, the book goes on to look at metal-catalyzed borylation of alkanes and arenas, coupling reactions and rhodium-catalyzed additions of boronic acids to alkenes and carbonyl compounds. There follows chapters on copper-promoted C-O and C-N cross-coupling of boronic acids, recent applications in organic synthesis, as well as alpha-haloalkylboronic esters in asymmetric synthesis. Later sections deal with cycloadditions, organoboronic acids, oxazaborolidines as asymmetric inducers, and boronic acid based receptors and sensors. The whole is rounded off with experimental procedures, making this invaluable reading for organic, catalytic and medicinal chemists, as well as those working in organometallics.
Download or read book Advanced Concepts in Fluorescence Sensing written by Chris D. Geddes and published by Springer Science & Business Media. This book was released on 2010-07-18 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, fluorescence has become the dominant tool in biotechnology and medical imaging. These exciting advances have been underpinned by the advances in time-resolved techniques and instrumentation, probe design, chemical / biochemical sensing, coupled with our furthered knowledge in biology. Complementary volumes 9 & 10, Advanced Concepts of Fluorescence Sensing: Small Molecule Sensing and Advanced Concepts of Fluorescence Sensing: Macromolecular Sensing, aim to summarize the current state of the art in fluorescent sensing. For this reason, Drs. Geddes and Lakowicz have invited chapters, encompassing a broad range of fluorescence sensing techniques. Some chapters deal with small molecule sensors, such as for anions, cations, and CO2, while others summarize recent advances in protein-based and macromolecular sensors. The Editors have, however, not included DNA or RNA based sensing in this volume, as this were reviewed in Volume 7 and is to be the subject of a more detailed volume in the near future.
Download or read book Design and Synthesis of Boronic Acid based Sensors for Microarray Analysis and FRET detection to Study Carbohydrates written by Yilin Wang and published by . This book was released on 2012 with total page 83 pages. Available in PDF, EPUB and Kindle. Book excerpt: Carbohydrates play significant roles in various biological and pathological processes such as cancer metastasis and inflammation. They participate in various signaling pathways and are able to bind to a litany of biological receptors such as proteins and viruses. Biologists have known for decades that the structures of glycans change with the onset of cancer and inflammation. Due to these important activities, carbohydrate sensing has long been a focus of research. One example of such a carbohydrate sensor is boronic acid-based sensors. Boronic acid-based sensors are of particular interest due to their selectivity for 1,2- or 1,3-diols. A variety of techniques have been employed for detection. Two techniques that have rarely been applied are high-throughput microarray analysis and FRET detection. In this thesis, we present work based on the development of a boronic acid-based carbohydrate sensor that will be used for microarray analysis and the development of various fluorophore-tagged boronic acids as donor/acceptor pairs for FRET detection. Herein, we describe the design and development of these compounds as well as initial results in their application for the detection of saccharides involved in important biological properties.
Download or read book Glucose Sensing written by Chris D. Geddes and published by Springer. This book was released on 2014-12-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential reference for any laboratory working in the analytical fluorescence glucose sensing field. The increasing importance of these techniques is typified in one emerging area by developing non-invasive and continuous approaches for physiological glucose monitoring. This volume incorporates analytical fluorescence-based glucose sensing reviews, specialized enough to be attractive to professional researchers, yet appealing to a wider audience of scientists in related disciplines of fluorescence.
Download or read book Novel Chemical Sensors Based on Boronic Acids for Glucose Detection written by Danielle Bruen and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Boronic acid (BA) derivatives have been exploited for their strong and reversible interactions with diol-containing compounds for the recognition of saccharides, such as glucose. Combining BA groups and fluorescent moieties can allow for sugar concentrations to be monitored by changes in fluorescence. In this thesis, two approaches based on BA sensing capabilities are investigated. In a direct sensing approach, the BA group is covalently attached to the fluorescent reporter group. Conversely, in an indirect sensing approach, a two-component system is created when the BA group and fluorophore are incorporated in to separate molecules. A direct sensing approach is described in Chapter 2, where the BA derivatives employed contain a quinoline-5-carboxylic acid functionality. These BA fluorescent sensors were investigated for their glucose sensing capabilities in solutions of various pH and when immobilised on to a 'lens-like' platform. An indirect sensing approach is described in Chapter 3, where a BA-cationic pyrimidinium molecule, induced fluorescence quenching in an anionic fluorophore (7-hydroxycoumarin). On introducing glucose, the fluorescence was recovered. This sensing system was investigated in solutions of various pH. Chapter 4 details the synthesis of a new family of BA-monomers. These monomers were characterised by 11B NMR and fluorescence in the absence and presence of glucose. In Chapter 5, the BA-monomers described in Chapter 4 were investigated for indirect sensing with the anionic fluorophore pyranine in solution and in hydrogels. Finally, in Chapter 6, additional strategies for the integration of a two-component sensing in to hydrogel matrices are investigated. The aim of this research is the development of novel sensing systems that could be integrated in to a continuous glucose-monitoring device. Such a platform could offer diabetics personal control over monitoring their glucose levels, to aid the prevention of the side effects associated with the disease.
Download or read book Cell based Biosensors written by Qingjun Liu and published by Artech House. This book was released on 2009-10 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by recognized experts the field, this leading-edge resource is the first book to systematically introduce the concept, technology, and development of cell-based biosensors. You find details on the latest cell-based biosensor models and novel micro-structure biosensor techniques. Taking an interdisciplinary approach, this unique volume presents the latest innovative applications of cell-based biosensors in a variety of biomedical fields. The book also explores future trends of cell-based biosensors, including integrated chips, nanotechnology and microfluidics. Over 140 illustrations help clarify key topics throughout the book.
Download or read book Transforming Glycoscience written by National Research Council and published by National Academies Press. This book was released on 2012-10-23 with total page 171 pages. Available in PDF, EPUB and Kindle. Book excerpt: A new focus on glycoscience, a field that explores the structures and functions of sugars, promises great advances in areas as diverse as medicine, energy generation, and materials science, this report finds. Glycans-also known as carbohydrates, saccharides, or simply as sugars-play central roles in many biological processes and have properties useful in an array of applications. However, glycans have received little attention from the research community due to a lack of tools to probe their often complex structures and properties. Transforming Glycoscience: A Roadmap for the Future presents a roadmap for transforming glycoscience from a field dominated by specialists to a widely studied and integrated discipline, which could lead to a more complete understanding of glycans and help solve key challenges in diverse fields.
Download or read book Boronic Acids as Optical Chemosensors for Saccharides and Phosphate Related Analytes written by Andrej Penavic and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the recent years, science has faced and is till facing numerous challenges associated with climate changes, environment pollution, food production, and access to inexpensive healthcare to name a few. These challenges have also created opportunities for fundamental research and development of new technologies that could alleviate the above problems. One example of such research activities is also the research and development of materials and methods useful for detecting and analyzing food or beverage components, environmental pollutants, or markers of important biological processes. Within this general field, molecules and materials that can be used as fluorescence-based sensors are very attractive. This is because of their potential for superior sensitivity at low cost and the fact that their operation does not require expensive equipment and highly trained personnel. Thus, the synthesis and investigation of supramolecular sensors that respond to analytes such as saccharides in food or beverage sources, or phosphates that are widely used as fertilizers and are also the source of eutrophication of water bodies are of both fundamental and practical importance. This dissertation describes the studies focused on supramolecular fluorescent chemosensors that are able to distinguish among various analytes that are structurally similar. We have synthesized and studied chemosensors for sensing of saccharides and their mixtures in beverages, as well as self-assembly based sensors for the detection of biologically important phosphate anions. Here, the sensing of phosphate-type anions is not only important for monitoring of eutrophication agents (orthophosphate and in lesser extent also oligo-/polyphosphates), but also for future application in biotechnologies such as quantitative polymerase chain reaction (qPCR). We hope that our contributions to research in sensors would contribute to further advancement of our knowledge and potential practical applications.
