Download or read book Development of an Automated 3D Flow focusing Microfluidic Platform for Single Cell Sorting written by Yingkai Lyu and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Analysis on Microfluidics written by Jin-Ming Lin and published by Springer. This book was released on 2017-10-25 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a detailed overview of the design, formatting, application, and development of microfluidic chips in the context of cell biology research, enumerating each element involved in microfluidics-based cell analysis, discussing its history, status quo, and future prospects, It also offers an extensive review of the research completed in the past decade, including numerous color figures. The individual chapters are based on the respective authors' studies and experiences, providing tips from the frontline to help researchers overcome bottlenecks in their own work. It highlights a number of cutting-edge techniques, such as 3D cell culture, microfluidic droplet technique, and microfluidic chip-mass spectrometry interfaces, offering a first-hand impression of the latest trends in the field and suggesting new research directions. Serving as both an elementary introduction and advanced guidebook, the book interests and inspires scholars and students who are currently studying microfluidics-based cell analysis methods as well as those who wish to do so.
Download or read book Self Learning Microfluidic Platform for Single Cell Imaging and Classification in Flow written by Iordania Constantinou and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Single-cell analysis commonly requires the confinement of cell suspensions in an analysis chamber or the precise positioning of single cells in small channels. Hydrodynamic flow focusing has been broadly utilized to achieve stream confinement in microchannels for such applications. As imaging flow cytometry gains popularity, the need for imaging-compatible microfluidic devices that allow for precise confinement of single cells in small volumes becomes increasingly important. At the same time, high-throughput single-cell imaging of cell populations produces vast amounts of complex data, which gives rise to the need for versatile algorithms for image analysis. In this work, we present a microfluidics-based platform for single-cell imaging in-flow and subsequent image analysis using variational autoencoders for unsupervised characterization of cellular mixtures. We use simple and robust Y-shaped microfluidic devices and demonstrate precise 3D particle confinement towards the microscope slide for high-resolution imaging. To demonstrate applicability, we use these devices to confine heterogeneous mixtures of yeast species, brightfield-image them in-flow and demonstrate fully unsupervised, as well as few-shot classification of single-cell images with 88% accuracy.
Download or read book Microtechnology for Cell Manipulation and Sorting written by Wonhee Lee and published by Springer. This book was released on 2016-10-05 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book delves into the recent developments in the microscale and microfluidic technologies that allow manipulation at the single and cell aggregate level. Expert authors review the dominant mechanisms that manipulate and sort biological structures, making this a state-of-the-art overview of conventional cell sorting techniques, the principles of microfluidics, and of microfluidic devices. All chapters highlight the benefits and drawbacks of each technique they discuss, which include magnetic, electrical, optical, acoustic, gravity/sedimentation, inertial, deformability, and aqueous two-phase systems as the dominant mechanisms utilized by microfluidic devices to handle biological samples. Each chapter explains the physics of the mechanism at work, and reviews common geometries and devices to help readers decide the type of style of device required for various applications. This book is appropriate for graduate-level biomedical engineering and analytical chemistry students, as well as engineers and scientists working in the biotechnology industry.
Download or read book Development of a Multisectorial Electroactive Nanowell Platform for Selective Single cell Sorting and Quantification written by Lukas Menze and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The rapid isolation of specific rare target cells is of rising importance in current targeted patient treatment plans. Common cell sorting approaches such as stream-based dielectrophoresis (DEP) based cell sorters are limited by their spatial dimensions as only a number of cell types corresponding to the number of output channels of the platform can be sorted. In this thesis, a microfluidic platform for selective single-cell sorting and subsequent quantification of sorted cells is presented, which can be upscaled to a high number of unique cell types without facing the same technical difficulties as previously developed devices. The platform consists of interdigitated electrodes (IDEs) and uses dielectrophoresis to capture target cells in a layer of 10 000 nanoliter wells placed on top. By splitting the design into 10 individually addressable IDE sectors, a large number of different cell types can be captured. This sectorial approach is highly modifiable and allows for complex samples to be captured over different sectors instead of requiring separate output channels for each cell type. The microfluidic behaviour of the platform regarding flow rate and DEP signal strength was examined to determine valid parameters for cell sorting and capture. A clinically relevant mixed sample of benign (MCF-10A) and malignant (MDA-MB-231) breast cells was used to validate the cell sorting performance of the platform and a target to non-target sorting accuracy of over 95\% could be achieved. To monitor sector occupancy and determine how much sample has been sorted, the capabilities of electrochemical impedance spectroscopy were examined. Experimental results revealed that impedimetric measurements can be used to quantify the number of captured cells, removing the need for an additional cell counting structure on the platform and hence reducing system complexity. Lastly, it was discussed how the presented microfluidic platform could be potentially expanded to facilitate single-cell RNA sequencing. The inability of current sequencing platforms to selectively capture and sequence cells from a mixed sample is a major problem of these designs. Since the platform presented in this thesis solves this problem, it may be a good candidate for future on-chip single-cell sequencing. Overall, the presented microfluidic cell sorting platform shows great promise to be used as either a point-of-care device or in clinical environments where reliable sorting of varying cell samples is important.
Download or read book Functional Metagenomics Tools and Applications written by Trevor C. Charles and published by Springer. This book was released on 2017-10-09 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book, the latest tools available for functional metagenomics research are described.This research enables scientists to directly access the genomes from diverse microbial genomes at one time and study these “metagenomes”. Using the modern tools of genome sequencing and cloning, researchers have now been able to harness this astounding metagenomic diversity to understand and exploit the diverse functions of microorganisms. Leading scientists from around the world demonstrate how these approaches have been applied in many different settings, including aquatic and terrestrial habitats, microbiomes, and many more environments. This is a highly informative and carefully presented book, providing microbiologists with a summary of the latest functional metagenomics literature on all specific habitats.
Download or read book High Throughput Microfluidic Technologies for Cell Separation and Single cell Analysis written by Lidan Wu (Ph. D.) and published by . This book was released on 2016 with total page 102 pages. Available in PDF, EPUB and Kindle. Book excerpt: The heterogeneity of individual cellular behavior in response to physical and chemical stimuli has raised increasing attention in many biological processes. There is great incentive in developing techniques for high throughput single-cell measurements and manipulations. Particularly, cell size has been recognized as an important parameter in single cell study and pericellular protease activity plays a key role in regulating the microenvironment of individual cells. Therefore, this thesis focuses on establishing new methods to address the issues of cell size and single cell protease measurement. We first develop a size-based cell separation technique using Dean-coupled inertial microfluidic sorter. Separation of cells by size before downstream assays might be beneficial in simplifying the system and facilitating the discovery of rare subpopulations through enrichment of cells with certain size range or cell cycle phase. By investigating the particle focusing and separation mechanisms in curved microfluidic channel, we develop a novel design of inertial microfluidic sorter with higher separation resolution and then demonstrate its capacity in leukocyte isolation from blood. This novel cell sorter would be a promising alternative to many other cell separation problems. We then establish a microfluidic platform for functional measurement of single cell pericellular proteases, including both those secreted and expressed on cell surface. We apply the platform to studying the PMA-mediated protease response of HepG2 cells at single-cell level and reveal the diversity in the dynamic patterns of single-cell protease activity profile upon drug stimulation. We also present the preliminary exploration of single-cell protease activity behavior in anticancer drug resistance development. Lastly, we explore the applicability of our platform for single-cell shedding measurement. Protease-mediated molecular shedding is one of the key mechanisms through which individual cells actively regulate their own microenvironment. However, the amount of molecules being shed for individual cells is extremely low, posing significant challenges in detecting shedding quantitatively. By means of analytical analysis and numerical simulations, we investigate the intrinsic noise of low-abundance molecule detection. Experimental characterizations have also been performed to evaluate the impact of practical factors on actual readout variation.
Download or read book Development of Multi purpose Microfluidic Platform for Single cell and Pairwise Cell Interaction Assay written by Young-Ji Kim and published by . This book was released on 2009 with total page 95 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microscale Acoustofluidics written by Thomas Laurell and published by Royal Society of Chemistry. This book was released on 2014-12-08 with total page 593 pages. Available in PDF, EPUB and Kindle. Book excerpt: The manipulation of cells and microparticles within microfluidic systems using external forces is valuable for many microscale analytical and bioanalytical applications. Acoustofluidics is the ultrasound-based external forcing of microparticles with microfluidic systems. It has gained much interest because it allows for the simple label-free separation of microparticles based on their mechanical properties without affecting the microparticles themselves. Microscale Acoustofluidics provides an introduction to the field providing the background to the fundamental physics including chapters on governing equations in microfluidics and perturbation theory and ultrasound resonances, acoustic radiation force on small particles, continuum mechanics for ultrasonic particle manipulation, and piezoelectricity and application to the excitation of acoustic fields for ultrasonic particle manipulation. The book also provides information on the design and characterization of ultrasonic particle manipulation devices as well as applications in acoustic trapping and immunoassays. Written by leading experts in the field, the book will appeal to postgraduate students and researchers interested in microfluidics and lab-on-a-chip applications.
Download or read book Microfluidics for Single Molecule Detection and Material Processing written by Sung Min Hong and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: In the cancer research, it is important to understand protein dynamics which are involved in cell signaling. Therefore, particular protein detection and analysis of target protein behavior are indispensable for current basic cancer research. However, it usually performed by conventional biochemical approaches, which require long process time and a large amount of samples. We have been developed the new applications based on microfluidics and Raster image Correlation spectroscopy (RICS) techniques. A simple microfluidic 3D hydrodynamic flow focusing device has been developed for quantitative determinations of target protein concentrations. The analyte stream was pinched not only horizontally, but also vertically by two sheath streams by introducing step depth cross junction structure. As a result, a triangular cross-sectional flow profile was formed and the laser was focused on the top of the triangular shaped analyte stream. Through this approach, the target protein concentration was successfully determined in cell lysate samples. The RICS technique has been applied to characterize the dynamics of protein 53 (p53) in living cells before and after the treatment with DNA damaging agents. P53 tagged with Green Fluores-cent Protein (GFP) were incubated with and without DNA damaging agents, cisplatin or eptoposide. Then, the diffusion coefficient of GFP-p53 was determined by RICS and it was significantly reduced after the drug treatment while that of the one without drug treatment was not. It is suggested that the drugs induced the interaction of p53 with either other proteins or DNA. This result demonstrates that RICS is able to detect protein-protein or protein-DNA interactions in living cells and it may be useful for the drug screening. As another application of microfluidics, an integrated microfluidic platform was developed for generating collagen microspheres with encapsulation of viable cells. The platform integrated four automated functions on a microfluidic chip, (1) collagen solution cooling system, (2) cell-in-collagen microdroplet generation, (3) collagen microdroplet polymerization, and (4) incubation and extraction of the microspheres. This platform provided a high throughput and easy way to generate uniform dimensions of collagen microspheres encapsulating viable cells that were able to proliferate for more than 1 week.
Download or read book New Microfluidic Platforms for Cell Studies written by Irena Barbulovic-Nad and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Microflow Cytometer written by Frances S. Ligler and published by Pan Stanford Publishing. This book was released on 2010-05-31 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Great book! Excellent compilation. From history of the very early days of flow cytometers to the latest unique unconventional microflow cytometers. From commercialization philosophy to cutting edge engineering designs. From fluid mechanics to optics to electronic circuit considerations. Well balanced and comprehensive."--Shuichi Takayama University of Michigan, USA.
Download or read book Toward Accessible Microfluidics written by Vinay Vishwas Abhyankar and published by . This book was released on 2008 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microfluidics written by Yu Song and published by John Wiley & Sons. This book was released on 2018-05-07 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first book offering a global overview of fundamental microfluidics and the wide range of possible applications, for example, in chemistry, biology, and biomedical science. As such, it summarizes recent progress in microfluidics, including its origin and development, the theoretical fundamentals, and fabrication techniques for microfluidic devices. The book also comprehensively covers the fluid mechanics, physics and chemistry as well as applications in such different fields as detection and synthesis of inorganic and organic materials. A useful reference for non-specialists and a basic guideline for research scientists and technicians already active in this field or intending to work in microfluidics.
Download or read book Microfluidics for Single Cell Analysis written by Jin-Ming Lin and published by Springer Nature. This book was released on 2019-08-28 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.
Download or read book Development of a Microfluidic Platform for Multicellular Tumour Spheroid Assays written by Kay Seonaid McMillan and published by . This book was released on 2016 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics is a valuable technology for a variety of different biomedical applications. In particular, within cancer research, it can be used to improve upon currently used in vitro screening assays by facilitating the use of 3D cell culture models. One of these models is the multicellular tumour spheroid (MCTS), which provides a more accurate reflection of the tumour microenvironment in vivo by reproducing the cell to cell contact, the development of a nutritional gradient and the formation of a heterogeneous population of cells. Therefore, the MCTS provides a more physiologically relevant in vitro model for testing the efficacy of treatments at the preclinical level. Currently, methods for the formation and culture of spheroids have several limitations, including being labour intensive, being low throughput, producing shear stress towards cells and the hanging drop system being unstable to physical shocks. Recently, microfluidics (especially droplet microfluidics) has been employed for the culture and screening of spheroids, providing a high-throughput methodology which only requires small volumes of fluids and small numbers of cells. However, current issues with droplet microfluidics include complicated droplet gelation procedures and short cell culture times.In this thesis, the use of microfluidic technologies as an approach for spheroid formation and culture are investigated with the aim to create a platform for radiotherapeutic and chemotherapeutic treatment of spheroids using cell lines. Initially, the use of emulsion technology at the macro scale was evaluated to determine the best conditions for spheroid culture. Once this was achieved the spheroids were compared to spheroids using a traditional method and radiotherapeutic treatment was conducted. Subsequently, avenues for miniaturising the developed emulsion-based methods were studied to provide a microfluidic technology. Finally, along with identifying the optimal culture conditions using hydrogels, a microfluidic system that integrated both droplet and single phase microfluidics features was developed for the formation and culture of spheroids. Using the latter, proof of principle experiments were conducted to demonstrate the suitability of the platform for both chemotherapeutic and radiotherapeutic assays within the same device.
Download or read book Development and Application of a Microfluidic Platform for Single cell Cultivation of CHO Suspension Cell Lines written by Julian Schmitz and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
