Download or read book Development of a Single Nucleotide Polymorphism SNP Assay for Rapid Genotyping of Elk Cervus Canadensis Based on Non invasively Collected Samples written by Gunmeet Kaur Bali and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent decades, non-invasively collected DNA samples have become an essential tool for wildlife genetic monitoring and conservation. While microsatellites have been traditionally used to genotype non-invasive genetic samples, single nucleotide polymorphisms (SNPs) offer several potential advantages over microsatellites. However, high-quantity DNA requirements of most SNP-genotyping platforms have posed a challenge to the use of SNPs for noninvasively collected DNA samples. We used a nanofluidic Integrated Fluidic Circuit (IFC)-based SNP genotyping technology (Fluidigm), which had been used previously on noninvasively collected DNA samples from carnivores. We developed a panel of 96 SNP loci for a Fluidigm platform and validated this assay for genotyping low quantity and quality DNA samples (fecal pellets) obtained from elk (Cervus canadensis). We initially tested 168 SNP loci on 94 high-quality (tissue, blood) samples (29 C. c. nannodes, 33 C. c. roosevelti, and 32 C. c. nelsoni), and selected a final set of 96 highly informative SNP loci based on performance and power to resolve individuals and discriminate subspecies. We then tested this panel of 96 SNPs on serial dilutions of the same high-quality samples (to simulate low quantity and quality DNA) as well as on 40 fecal DNA samples run in duplicate. Call rates were >98% and genotyping error rates
Download or read book Single Nucleotide Polymorphisms written by Anton A. Komar and published by Humana Press. This book was released on 2012-02-25 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, single nucleotide polymorphisms have received increased and special attention in a rapidly developing field of personalized medicine and drug treatment. Comprising more than eighty percent of all known polymorphisms, single nucleotide polymorphisms (SNPs) are primarily responsible for phenotypic differences between individuals, and have been suggested to affect the development of diseases in humans and the response to drug treatment and environmental stress. In Single Nucleotide Polymorphisms: Methods and Protocols, Second Edition, expert researchers explore the latest advances in this area, highlighting the substantial progress that has been made in SNP genotyping, examining recent developments in high-throughput genotyping approaches, and exploring our new understanding of the impact of SNPs on gene function. Chapters address the impact of SNPs on phenotype, examine SNP databases, look at methods that have been applied for SNP bioinformatics discovery and analysis, and discuss advanced experimental approaches used for SNP detection. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Current and innovative, Single Nucleotide Polymorphisms: Methods and Protocols, Second Edition is an essential guidebook for individual researchers as well as institutions and companies working in the field.
Download or read book Development of Single Nucleotide Polymorphism SNP Markers for Rapid Inexpensive and Standardized Identification of Pallid Scaphirhynchus Albus and Shovelnose S Platorynchus Sturgeon Larvae written by Matthew S. Krampe and published by . This book was released on 2011 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this project was to develop inexpensive, standardized, and high throughput Single Nucleotide Polymorphism (SNP) markers that discriminate between pallid (Scaphirhynchus albus ) and shovelnose (S. platorynchus ) sturgeon for use as a larval identification tool. A total of 67 polymorphic sites was identified in DNA sequences from three genes: Recombination Activating Gene-1, Beta Actin, and Beta-2-Microglobulin. Allele frequencies from the 10 most variable SNPs were characterized for both pallid and shovelnose sturgeon in three geographically separated populations throughout the range of the pallid sturgeon. To create a standardized method of genotyping SNPs for larval pallid and shovelnose sturgeon, 5' nuclease allelic discrimination (TaqMan) assays were designed for two unlinked SNPs that exhibited the greatest allele frequency differences between species. A power analysis compared these SNP loci and their diagnostic power for species discrimination compared to sixteen microsatellite loci currently used for species discrimination (Schrey et al. 2007) One SNP locus was the most powerful marker for species identification in the upper and middle Missouri River. This study provides practical genetic tools for species discrimination between pallid and shovelnose that will facilitate understanding addressing questions that were previously too costly, labor intensive or technically challenging to answer.
Download or read book Critical Needs and Gaps in Understanding Prevention Amelioration and Resolution of Lyme and Other Tick Borne Diseases written by Institute of Medicine and published by National Academies Press. This book was released on 2011-07-01 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: A single tick bite can have debilitating consequences. Lyme disease is the most common disease carried by ticks in the United States, and the number of those afflicted is growing steadily. If left untreated, the diseases carried by ticks-known as tick-borne diseases-can cause severe pain, fatigue, neurological problems, and other serious health problems. The Institute of Medicine held a workshop October 11-12, 2010, to examine the state of the science in Lyme disease and other tick-borne diseases.
Download or read book Bears of the World written by Vincenzo Penteriani and published by Cambridge University Press. This book was released on 2020-11-30 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bears have fascinated people since ancient times. The relationship between bears and humans dates back thousands of years, during which time we have also competed with bears for shelter and food. In modern times, bears have come under pressure through encroachment on their habitats, climate change, and illegal trade in their body parts, including the Asian bile bear market. The IUCN lists six bears as vulnerable or endangered, and even the least concern species, such as the brown bear, are at risk of extirpation in certain countries. The poaching and international trade of these most threatened populations are prohibited, but still ongoing. Covering all bears species worldwide, this beautifully illustrated volume brings together the contributions of 200 international bear experts on the ecology, conservation status, and management of the Ursidae family. It reveals the fascinating long history of interactions between humans and bears and the threats affecting these charismatic species.
Download or read book European Ungulates and Their Management in the 21st Century written by Marco Apollonio and published by Cambridge University Press. This book was released on 2010-02-04 with total page 619 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first book to summarise management objectives for ungulates across Europe.
Download or read book Sika Deer written by Dale R. McCullough and published by Springer Science & Business Media. This book was released on 2008-12-05 with total page 665 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sika deer, the graceful spotted deer of Japanese and Chinese art, originally were native to Asia from far-east Russia to Vietnam to the islands of Japan and Taiwan. They are widely raised in captivity to supply velvet antler for traditional medicine. They also were introduced to Europe, North America, and New Zealand, where they compete or interbreed with native deer. Sika deer typically occupy lowland hardwood forests with low winter snow depths, where they thrive in sites disturbed by fire, storm, or logging. In high numbers they can severely impact vegetation though overgrazing, stripping bark from trees and damaging crop fields and forest plantations. Their numbers are high in many parts of Japan, moderate in Russia, and reduced or extinct in the wild in China, Korea, Vietnam, and Taiwan. This book explores their basic biology, behavior, and ecology, including management for sport hunting, conservation or recovery of threatened populations, and resolution of conflict with humans in native and introduced lands.
Download or read book Escherichia coli in the Americas written by Alfredo G. Torres and published by Springer. This book was released on 2016-10-14 with total page 393 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacterial diarrheal diseases remain an important leading cause of preventable death, especially among children under five in developing countries. In the American continent, diarrheal disease and other health complications caused by Escherichia coli constitute a major public health problem, and, therefore, several research groups have dedicated their effort to understand this pathogen and provide feasible solutions to prevent, treat and reduce E. coli infections. The Latin American Coalition for Escherichia coli Research (LACER) was created as a multidisciplinary network of international research groups working with E. coli with the ultimate goal of advancing understanding of E. coli, and to prepare the next generation of American E. coli investigators. As such, this book compiles the knowledge of these investigators about E. coli, a commensal bacteria living inside its host, and a pathogen causing disease in animals and humans. Escherichia coli in the Americas contains a series of 15 chapters written by experts, covering basic concepts regarding the different categories of E. coli, including their environmental niche, virulence mechanisms, host reservoir, and disease outcomes, as well as diagnosis, vaccine development and treatment. This book's target audience include trainees and students learning about the basic and clinical aspects of E. coli pathogenesis, as well as experts around the globe who wish to learn more about this pathogen and the public health impact this bacteria has in America.
Download or read book Wildlife DNA Analysis written by Adrian Linacre and published by John Wiley & Sons. This book was released on 2013-03-27 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Clearly structured throughout, the introduction highlights the different types of crime where these techniques are regularly used. This chapter includes a discussion as to who performs forensic wildlife examinations, the standardisation and validation of methods, and the role of the expert witness in this type of alleged crime. This is followed by a detailed section on the science behind DNA typing including the problems in isolating DNA from trace material and subsequent genetic analysis are also covered. The book then undertakes a comprehensive review of species testing using DNA, including a step-by-step guide to sequence comparisons. A comparison of the different markers used in species testing highlights the criteria for a genetic marker. A full set of case histories illustrates the use of the different markers used. The book details the use of genetic markers to link two or more hairs/feather/leaves/needles to the same individual organism and the software used in population assignment. The problems and possibilities in isolating markers, along with the construction of allele databases are discussed in this chapter. The book concludes with evaluation and reporting of genetic evidence in wildlife forensic science illustrated by examples of witness statements.
Download or read book Chromosome Structure and Aberrations written by Tariq Ahmad Bhat and published by Springer. This book was released on 2017-02-08 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a compilation of various chapters contributed by a group of leading researchers from different countries and covering up to date information based on published reports and personal experience of authors in the field of cytogenetics. Beginning with the introduction of chromosome, the subsequent chapters on organization of genetic material, karyotype evolution, structural and numerical variations in chromosomes, B-chromosomes and chromosomal aberrations provide an in-depth knowledge and easy understanding of the subject matter. A special feature of the book is the inclusion of a series of chapters on various types of chromosomal aberrations and their impact on breeding behaviour and crop improvement. The possible mechanism, their consequences and role in genetic analysis has been emphasized in these chapters. A few chapters have also been dedicated on various techniques routinely used in the laboratory by students and researchers. Each chapter ends with an extensive bibliography so that the students and researchers may find it relevant to consult more literature on the subject than a book of this size can offer. The book is intended to fulfill the needs of undergraduate and post graduate students of botany, zoology and agriculture besides, teachers and researchers engaged in the field of genetics, cytogenetics, and molecular genetics. In general the readers will find each chapter of the book informative and easy to understand.
Download or read book Foundations of Restoration Ecology written by Society for Ecological Restoration International and published by Island Press. This book was released on 2016-11 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Society for Ecological Restoration"--Cover.
Download or read book Forensic Science in Wildlife Investigations written by Adrian Linacre and published by CRC Press. This book was released on 2009-03-12 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bringing together the most recent literature, this book provides an in-depth look at the field of wildlife forensic examination. Offering practical guidance, it helps investigators and lab technicians decide on best methods, including a determination of when basic microscopy is sufficient, when DNA testing should occur, and what tests or combination of tests should be executed in a particular circumstance. The text illustrates how to identify the species and geographic region of origin of an unknown sample. International contributors separate truth from myth in providing information and insight regarding the wide scope of endangered animal trafficking for meats, arts, curios, aphrodisiacs, and traditional medicines.Selected Contents: CITES
Download or read book Wildlife Biodiversity Conservation written by Susan C. Underkoffler and published by Springer Nature. This book was released on 2021-06-26 with total page 485 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book addresses the multidisciplinary challenges in biodiversity conservation with a focus on wildlife crime and how forensic tools can be applied to protect species and preserve ecosystems. Illustrated by numerous case studies covering different geographical regions and species the book introduces to the fundamentals of biodiversity conflicts, outlines the unique challenges of wildlife crime scenes and reviews latest techniques in environmental forensics, such as DNA metagenomics. In addition, the volume explores the socio-economic perspective of biodiversity protection and provides an overview of national and international conservation laws. The field of conservation medicine stresses the importance of recognizing that human health, animal health, and ecosystem health are inextricably interdependent and the book serves as important contribution towards achieving the UN Sustainable Developmental Goals, in particular SDG 15, Life on Land. The book addresses graduate students, scientists and veterinary professionals working in wildlife research and conservation biology.
Download or read book Forensic Analysis written by Ian R. Freckelton and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Forensic Analysis - Scientific and Medical Techniques and Evidence under the Microscope is an edited collection with contributions from scholars in ten countries, containing cutting-edge analyses of diverse aspects of contemporary forensic science and forensic medicine. It spans forensic gait analysis evidence, forensic analysis in wildlife investigations, mitochondrial blood-typing, DNA profiling, probabilistic genotyping, toolmark analysis, forensic osteology, obstetric markers as a diagnostic tool, salivary analysis, pharmacogenetics, and forensic analysis of herbal drugs. This book provides information about the parameters of expertise in relation to a number of areas that are being utilised as a part of criminal investigations and that are coming before courts internationally or will soon do so. Thereby, it is hoped that rigor in the evaluation of such evidence will be enhanced, a fillip for developing standards will be provided, and the incidence of miscarriages of criminal justice will be minimised.
Download or read book Rapidly Evolving Genes and Genetic Systems written by Rama S. Singh and published by Oxford University Press. This book was released on 2012-06-28 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: A range of theories on the rates of evolution-from static to gradual to punctuated to quantum-have been developed, mostly by comparing morphological changes over geological timescales as described in the fossil record.
Download or read book Molecular Ecology written by Joanna R. Freeland and published by John Wiley & Sons. This book was released on 2006-03-30 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Ecology provides a comprehensive introduction to the many diverse aspects of this subject. The book unites theory with examples from a wide range of taxa in a logical and progressive manner, and its accessible writing style makes subjects such as population genetics and phylogenetics highly comprehensible to its readers. The first part of the book introduces the essential underpinnings of molecular ecology, starting with a review of genetics and a discussion of the molecular markers that are most frequently used in ecological research. This leads into an overview of population genetics in ecology. The second half of the book then moves on to specific applications of molecular ecology, covering phylogeography, behavioural ecology and conservation genetics. The final chapter looks at molecular ecology in a wider context by using a number of case studies that are relevant to various economic and social concerns, including wildlife forensics, agriculture, and overfishing * comprehensive overview of the different aspects of molecular ecology * attention to both theoretical and applied concerns * accessible writing style and logical structure * numerous up-to-date examples and references This will be an invaluable reference for those studying molecular ecology, population genetics, evolutionary biology, conservation genetics and behavioural ecology, as well as researchers working in these fields.
Download or read book Handbook of Statistical Genetics written by David J. Balding and published by John Wiley & Sons. This book was released on 2008-06-10 with total page 1616 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Handbook for Statistical Genetics is widely regarded as the reference work in the field. However, the field has developed considerably over the past three years. In particular the modeling of genetic networks has advanced considerably via the evolution of microarray analysis. As a consequence the 3rd edition of the handbook contains a much expanded section on Network Modeling, including 5 new chapters covering metabolic networks, graphical modeling and inference and simulation of pedigrees and genealogies. Other chapters new to the 3rd edition include Human Population Genetics, Genome-wide Association Studies, Family-based Association Studies, Pharmacogenetics, Epigenetics, Ethic and Insurance. As with the second Edition, the Handbook includes a glossary of terms, acronyms and abbreviations, and features extensive cross-referencing between the chapters, tying the different areas together. With heavy use of up-to-date examples, real-life case studies and references to web-based resources, this continues to be must-have reference in a vital area of research. Edited by the leading international authorities in the field. David Balding - Department of Epidemiology & Public Health, Imperial College An advisor for our Probability & Statistics series, Professor Balding is also a previous Wiley author, having written Weight-of-Evidence for Forensic DNA Profiles, as well as having edited the two previous editions of HSG. With over 20 years teaching experience, he’s also had dozens of articles published in numerous international journals. Martin Bishop – Head of the Bioinformatics Division at the HGMP Resource Centre As well as the first two editions of HSG, Dr Bishop has edited a number of introductory books on the application of informatics to molecular biology and genetics. He is the Associate Editor of the journal Bioinformatics and Managing Editor of Briefings in Bioinformatics. Chris Cannings – Division of Genomic Medicine, University of Sheffield With over 40 years teaching in the area, Professor Cannings has published over 100 papers and is on the editorial board of many related journals. Co-editor of the two previous editions of HSG, he also authored a book on this topic.
