Download or read book Development of a Rapid Method for Detection of Bloom forming Cyanobacteria and Algae in Selected New Jersey Waterbodies written by Ben Nyabwari Ondimu and published by . This book was released on 2010 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Early Detection of Cyanobacterial Toxins Using Genetic Methods written by J. Paul Rasmussen and published by American Water Works Association. This book was released on 2008 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: This research sought to develop and test rapid genetic methods to identify toxic cyanobacteria. The key objectives were to (1) conduct a literature review and industry questionnaire examining options for rapid genetic tests; (2) characterize and understand the genes involved in cylindrospermopsin and anatoxin production; (3) adapt conventional PCR assays to real-time PCR; (4) develop rapid field methods for DNA preparation; and (5) develop probes for toxin genes and testing in the field.
Download or read book Detection Methods for Cynobacterial toxins written by G A Codd and published by Elsevier. This book was released on 1994-01-01 with total page 202 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cyanobacteria, or blue-green algae, commonly occur in a variety of water types throughout the world. A variable, but, high proportion of the cyanobacterial blooms and scums, which can develop annually in lakes, reservoirs, canals and slow-flowing rivers, contain potent toxins. Although animal poisonings and human health problems associated with the ingestion of, or contact with, cyanobacterial scums have long been recognized, a developing understanding of the health hazards posed by the toxins requires that reliable, sensitive, specific and convenient methods are available for their detection and quantification.Detection methods for cynobacterial toxins looks at the application of biological, toxicological, biochemical and physicochemical techniques in studies of cyanobacterial toxins and at experimental methods that have identified at least 50 cyanobacterial compounds toxic to vertebrates.Academics, public health bodies, environmental protection agencies and water companies are all involved in monitoring cyanobacterial levels and effects, and this book will help to ensure that their research is not duplicated, is standardized and comparable, and that optimal progress can be made in an important area that is still in its developing stages.
Download or read book Detection Methods for Algae Protozoa and Helminths in Fresh and Drinking Water written by Franca Palumbo and published by John Wiley & Sons. This book was released on 2008-04-30 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the second book in the Water Quality Measurement Series. Itfocuses on the analytical aspects related to epidemiology,toxicology, sanitary, engineering and plant technology to providean integrated and clear strategy for carrying out surveillance,quality control, prevention and remedial measures. * Contains a significant number of tables, figures, colour andblack and white photographs and spectra * Offers workable answers to specific practical issues using acomprehensive and scientifically sound approach
Download or read book Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria written by Rainer Kurmayer and published by John Wiley & Sons. This book was released on 2017-06-29 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: A guide to state-of-the-art molecular tools for monitoring and managing the toxigenicity of cyanobacteria Runaway eutrophication and climate change has made the monitoring and management of toxigenic organisms in the world’s bodies of water more urgent than ever. In order to influence public policy regarding the detection and quantification of those organisms, it is incumbent upon scientists to raise the awareness of policy makers concerning the increased occurrence of toxigenic cyanobacteria and the threats they pose. As molecular methods can handle many samples in short time and help identify toxigenic organisms, they are reliable, cost-effective tools available for tracking toxigenic cyanobacteria worldwide. This volume arms scientists with the tools they need to track toxigenicity in surface waters and food supplies and, hopefully, to develop new techniques for managing the spread of toxic cyanobacteria. This handbook offers the first comprehensive treatment of molecular tools for monitoring toxigenic cyanobacteria. Growing out of the findings of the landmark European Cooperation in Science and Technology Cyanobacteria project (CYANOCOST), it provides detailed, practical coverage of the full array of available molecular tools and protocols, from water sampling, nucleic acid extraction, and downstream analysis—including PCR and qPCR based methods—to genotyping (DGGE), diagnostic microarrays, and community characterization using next-gen sequencing techniques. Offers an overview of the latest trends in the field, while providing a foundation for understanding and applying the tools and techniques described Provides detailed coverage of the full range of molecular tools currently available, with expert guidance on the analysis and interpretation of results Includes step-by-step guidance on standard operational procedures, including molecular tests used in environmental monitoring, with individual chapters devoted to each procedure Complements the published Handbook of Cyanobacterial Monitoring and Cyanotoxin Analysis from the CyanoCOST project This handbook is an indispensable working resource for scientists, lab technicians, and water management professionals and an excellent text/reference for graduate students and supervisors who use molecular tools. It will also be of great value to environmental health and protection officials and policy makers.
Download or read book Bloom forming Cyanobacteria and Other Phytoplankton in Northern New Jersey Freshwater Bodies written by Matthew J. Rienzo and published by . This book was released on 2012 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protocols for Cyanobacteria Sampling and Detection of Cyanotoxin written by N. Thajuddin and published by Springer Nature. This book was released on 2024-01-19 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: This protocol book provides detailed procedures for the isolation of cyanobacteria, extraction, quantification, and detection of cyanotoxins. It illustrates the sampling and processing of toxin-producing cyanobacteria in water and aquatic animal samples, detection of cyanotoxins from Anabaena, Anabaenopsis, Cylindrospermopsis, Microsystis, Microcystis, Nodularia, Nostoc, Schizotrix, Lyngbya, Raphidiopsis, Oscillatoria, Planktothrix in aquatic resources. It also covers toxicity analysis by various bioassay protocols, and in vitro and insilico analysis methods. The book also reviews the methods for cyanotoxin extraction, detection, and quantification by various tools including LC-MS/MS, HPLC, NMR, PCR, and HESI-MS/MS. A separate section is dedicated to the advanced methods in Cyanotoxin analysis including the Molecular Imprinting Method (MIM), Cellular signaling biosensor, Electrochemical sensor, Nanosensors, and screening of Polyketide synthase gene. The analysis of various toxin-producing genes like sxtA and mcy is also accounted for in this book. In a nutshell, the book gives comprehensive procedures about the basics and preliminary processes that are involved in sample collection to advanced methods incorporated into the well-explored and unexplored Cyanobacterial toxin. Consequently, this manual is useful for both beginners and advanced researchers, including postgraduate students, academicians, researchers, and scientists in the field of Cyanobacterial research.
Download or read book Algae Detection and Removal Strategies for Drinking Water Treatment Plants written by and published by American Water Works Association. This book was released on 2004 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual for conventional water treatment plants outlines monitoring strategies for detecting the onset of algae blooms in drinking water sources as well as treatment strategies for minimizing the adverse effects of algae on unit process performance and finished water quality. The manual draws on
Download or read book Detection Methods for Cynobacterial Toxins written by G. A. Codd and published by Woodhead Pub Limited. This book was released on 1994-01-01 with total page 202 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cyanobacteria, or blue-green algae, commonly occur in a variety of water types throughout the world. A high proportion of the cyanobacterial blooms and scums contain potent toxins. This volume looks at the application of biological, toxicological, biochemical and physicochemical techniques in studies of cyanobacterial toxins and at experimental methods that have identified at least 50 cyanobacterial compounds toxic to vertebrates.
Download or read book Bloom Forming Cyanobacteria and Other Phytoplankton in Northern New Jersey Freshwater Bodies written by Tin-Chun Chu and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Bloom-Forming Cyanobacteria and Other Phytoplankton in Northern New Jersey Freshwater Bodies.
Download or read book Freshwater Algae written by Edward G. Bellinger and published by John Wiley & Sons. This book was released on 2011-09-20 with total page 379 pages. Available in PDF, EPUB and Kindle. Book excerpt: Freshwater Algae: Identification and Use as Bioindicators provides a comprehensive guide to temperate freshwater algae, with additional information on key species in relation to environmental characteristics and implications for aquatic management. The book uniquely combines practical material on techniques and water quality management with basic algal taxonomy and the role of algae as bioindicators. Freshwater Algae: Identification and Use as Bioindicators is divided into two parts. Part I describes techniques for the sampling, measuring and observation of algae and then looks at the role of algae as bioindicators and the implications for aquatic management. Part II provides the identification of major genera and 250 important species. Well illustrated with numerous original illustrations and photographs, this reference work is essential reading for all practitioners and researchers concerned with assessing and managing the aquatic environment.
Download or read book Real time Coastal Observing Systems for Marine Ecosystem Dynamics and Harmful Algal Blooms written by Babin, Marcel and published by UNESCO. This book was released on 2008-06-05 with total page 880 pages. Available in PDF, EPUB and Kindle. Book excerpt: The proliferation of harmful phytoplankton in marine ecosystems can cause massive fish kills, contaminate seafood with toxins, impact local and regional economies and dramatically affect ecological balance. Real-time observations are essential for effective short-term operational forecasting, but observation and modelling systems are still being developed. This volume provides guidance for developing real-time and near real-time sensing systems for observing and predicting plankton dynamics, including harmful algal blooms, in coastal waters. The underlying theory is explained and current trends in research and monitoring are discussed.Topics covered include: coastal ecosystems and dynamics of harmful algal blooms; theory and practical applications of in situ and remotely sensed optical detection of microalgal distributions and composition; theory and practical applications of in situ biological and chemical sensors for targeted species and toxin detection; integrated observing systems and platforms for detection; diagnostic and predictive modelling of ecosystems and harmful algal blooms, including data assimilation techniques; observational needs for the public and government; and future directions for research and operations.
Download or read book Public Health Surveillance of Toxic Cyanobacteria in Freshwater Systems Using Remote Detection Methods written by Trina Nicole Mackie and published by . This book was released on 2010 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cyanotoxins, the group of toxic chemicals produced by the blue-green algae or cyanobacteria that can proliferate in fresh and salt-water, cause a range of harmful health effects including skin rashes, flu-like symptoms, nausea, diarrhea, tingling and nerve damage, liver damage, tumors, and death ([1, 2]). Although cyanobacteria are one of the oldest organisms on the planet, anthropogenic development (e.g. dams, water diversions, nutrient rich runoff from intensive agriculture, decreased impervious area from urbanization, etc.) has caused many watersheds to lose substantial water volume, suffer tremendous inputs of nutrients and other organic and inorganic pollutants, increase in temperature and overall become more suitable for the proliferation of harmful blooms of cyanobacteria. Cyanobacteria blooms are now increasingly prevalent in freshwaters as eutrophication becomes ever more common with human stressors, and climate change is likely to only further exacerbate this problem. Public health professionals are dependant upon early and dependable information on the presence, concentration, and location of cyanobacteria blooms in order to inform the public and reduce potential exposure. This research project evaluated the efficacy of remote sensing data to provide this kind of surveillance and early detection for characterizing the presence of toxic algae in freshwater systems. It explored the relevance of specific remote sensing techniques to freshwater cyanobacteria bloom identification. The various remote sensing platforms available for this kind of research vary in cost, swath coverage, spatial scale and spectral resolution. For this study, three different remote imagery platforms were compared in terms of their ability to identify surface blooms and to distinguish gradients in cell density or bloom intensity. This exploration of the application of remote sensing used a hyperspectral airborne sensor with high spatial resolution (SpecTIR), a multispectral satellite image also with high spatial resolution (IKONOS), and a lower spatial resolution multispectral satellite image (Landsat). Water sampling data (algal pigment concentrations, turbidity, transparency, and temperature) from known blue-green algae blooms dominated by microcystis aeruginosa on the Iron Gate and Copco Reservoirs on the Klamath River were used to evaluate and classify these different images. This research successfully used both satellite and airborne remotely sensed data to visualize where the medium or high density sections of the bloom were located, to quantify the intensity of the bloom in terms of area impacted, and to compare the intensity of the bloom at different dates in time. Remote sensing can provide a synoptic overview of the entire system, making it possible to truly assess relative bloom intensity. Furthermore the results indicate that when given the choice, the investment in higher spectral resolution should be chosen over higher spatial resolution as the former appears to provide more benefits in cyanobacteria detection.
Download or read book Cyanobacterial Harmful Algal Blooms State of the Science and Research Needs written by H. Kenneth Hudnell and published by Springer Science & Business Media. This book was released on 2008-03-13 with total page 955 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the ever-increasing incidence of harmful cyanobacterial algal blooms, this monograph has added urgency and will be essential reading for all sorts of researchers, from neuroscientists to cancer research specialists. The volume contains the proceedings of the 2005 International Symposium on Cyanobacterial Harmful Algal Blooms, and has been edited by H. Kenneth Hudnell, of the US Environmental Protection Agency. It contains much of the most recent research into the subject.
Download or read book Development of Monitoring and Treatment Technologies to Combat Harmful Algae Blooms written by Shardula Gawankar and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lake Erie has been affected by harmful algal blooms for decades. In 2014, this resulted in the plant having to shut down its intake after toxic cyanotoxins were found in source water. Such occurrences are becoming more common across the globe. U.S. EPA has established regulations for microcystin, the most common form of cyanotoxin. Climate change is predicted to increase the occurrence of other types of cyanotoxins, such as saxitoxins, which are not regulated by the U.S. EPA. Hence, the removal and monitoring of cyanotoxins, produced by harmful algae blooms, in water is of utmost importance to protect public health.The efficacy of oxidation varies greatly for each of the cyanotoxins due to their different chemical structures. There is presently no oxidation process that a water treatment plant can implement that is proven to simultaneously remove all the cyanotoxins (microcystin, saxitoxin, cylindrospermopsin, and anatoxin) from drinking water. Thus, water treatment plants that are currently designed to remove microcystins are not protected against all forms of cyanotoxins. The investigation of the removal of these cyanotoxins using innovative treatment technologies requires a detection method that is sensitive and capable of detecting all the variants of cyanotoxins. The detection of saxitoxin is particularly challenging as compared to other cyanotoxins due to its low molecular mass and highly polar nature. Hydrophilic interaction liquid chromatography coupled with mass spectrometry (HILIC-MS) has the ability to provide specific detection through mass differentiation, which makes it an ideal tool for the quantitative analysis of saxitoxin and its variants. Hence, a method was developed to extract and detect saxitoxin from water using HILIC-MS in conjunction with weak cation exchange solid phase extraction (SPE), to provide a sensitive and reliable quantification of saxitoxins. However, the application of LC/MS for the detection of cyanotoxins in treatment studies is not cost effective as the cost of instrumentation is high, its operation requires high skill, and cyanotoxin standards have limited access and are expensive. Hence, a screening technique has been developed which uses methylene blue to identify the reaction kinetics of persulfate and peroxide oxidation in the presence of ferrous chloride and to optimize parameters, which can be helpful in predicting the degradation of cyanotoxins under similar conditions. Catalyst activated persulfate and peroxide oxidation produce sulfate and hydroxyl radicals, which can degrade a wide range of recalcitrant chemicals and hence are preferred in water and wastewater treatment. The screening technique was validated by investigating the degradation of microcystin-LR. The notable advantages of developing this screening technique are: (i) reduced cost of analysis as methylene blue can be detected in real time by measuring its absorbance, and (ii) can perform multiple trials in short time due to ease of analysis. This screening technique was also applied to iron oxide coated ceramic membranes in combination with persulfate oxidation to understand the degradation kinetics.
Download or read book Guidelines for Safe Recreational Water Environments Coastal and fresh waters written by World Health Organization and published by World Health Organization. This book was released on 2003 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: The new guidelines are meant to protect public health, help evaluate development projects near freshwater and recreational sites and assess potential health aspects of recreational projects.
Download or read book Development of Optical Bio sensing Methods for Detection of Toxins Produced by Algae written by Rukaiah Al-Ammar and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
