Download or read book Development of a Confocal and Near field Scanning Optical Microscope for Polarization resolved Temporal Studies of Single Nano objects written by Michael Johannes Nasse and published by . This book was released on 2004 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: We present the development and application of a hybrid confocal and aperture near-field scanning optical microscope operating in reflection mode. It has been designed to work at cryogenic temperatures and in vacuum or with an exchange gas. Furthermore, it permits to split the fluorescence into two perpendicular polarization channels.We have studied various single nano-objects, such as molecules, CdSe/ZnSe nanocrystals, CdSe/ZnS nanorods and fluorescent nanospheres. As molecular samples we used diI-C18 molecules and molecules derived from anthracene. The ensemble absorption and emission spectra of the latter were measured, as well as their absorption cross-section and quantum yield in solution for the first time. Furthermore, we used time correlated fluorescence decay experiments that suggest the presence of different conformations in solution, already observed with similar molecules.We used our instrument in confocal mode to image, record time traces, and acquire some spectra of single nano-objects. Single nanospheres with a diameter of 27 nm are used to map the optical field distribution in the vicinity of the focal point of a microscope objective. The experimentally obtained images are qualitatively compared with a scalar model. The near-field scanning optical microscopy setup is validated using nanospheres with a diameter of 500 nm.The dynamic temporal behavior is examined with the help of time traces. The study is based on the autocorrelation function for single molecules, and using an on-off statistics for single nanocrystals/-rods. In the latter case a power law distribution of the on- and off-time probabilities is observed.

Download or read book Confocal Scanning Optical Microscopy and Related Imaging Systems written by Gordon S. Kino and published by Academic Press. This book was released on 1996-09-18 with total page 353 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers. The book concentrates mainly on two instruments: the Confocal Scanning Optical Microscope (CSOM), and the Optical Interference Microscope (OIM). A comprehensive discussion of the theory and design of the Near-Field Scanning Optical Microscope (NSOM) is also given. The text discusses the practical aspects of building a confocal scanning optical microscope or optical interference microscope, and the applications of these microscopes to phase imaging, biological imaging, and semiconductor inspection and metrology.A comprehensive theoretical discussion of the depth and transverse resolution is given with emphasis placed on the practical results of the theoretical calculations and how these can be used to help understand the operation of these microscopes. Provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers Explains many practical applications of scanning optical and interference microscopy in such diverse fields as biology and semiconductor metrology Discusses in theoretical terms the origin of the improved depth and transverse resolution of scanning optical and interference microscopes with emphasis on the practical results of the theoretical calculations Considers the practical aspects of building a confocal scanning or interference microscope and explores some of the design tradeoffs made for microscopes used in various applications Discusses the theory and design of near-field optical microscopes Explains phase imaging in the scanning optical and interference microscopes

Download or read book Nano optics and Near field Optical Microscopy written by Anatoly V. Zayats and published by Artech House. This book was released on 2009 with total page 379 pages. Available in PDF, EPUB and Kindle. Book excerpt: "This groundbreaking book focuses on near-field microscopy which has opened up optical processes at the nanoscale for direct inspection. Further, it explores the emerging area of nano-optics which promises to make possible optical microscopy with true nanometer resolution. This frontline resource helps you achieve high resolution optical imaging of biological species and functional materials. You also find guidance in the imaging of optical device operation and new nanophotonics functionalities"--EBL.

Download or read book Spatial Temporal Resolution in Near Field Optical Microscopy written by Hans Hallen and published by . This book was released on 1998 with total page 13 pages. Available in PDF, EPUB and Kindle. Book excerpt: This project elucidated and examined the unique science behind the spectral and temporal contrast of near field scanning optical microscopy, illuminated the technology this enabled, and considered the limits of simultaneous position, time and spectral resolution. Specifically, (1) High-resolution, quantitative measurements of excess carrier lifetime in silicon were acquired in a novel, all-optical technique. The contrast in the images was modeled and is of interest since the resolution is significantly shorter than the carrier diffusion length. (2) a Ti-sapphire laser has been constructed and modified to decrease pump power requirements so that measurements can be made before the carriers diffuse from under the probe. Novel electron-hole droplet effects are expected. (3) Spectroscopic nano-Raman images were acquired for the first time. The Raman data illustrated interesting manifestations of a near-field in comparisons with far-field spectroscopy. (4) The first and most thorough studies of the optical and thermal properties of near-field scanning optical microscope probes gave insights which led to the development of a new generation of high throughput probes. Other results reflect instrumentation advances: (5) A new constant linear motion system useful for NSOM or other probe microscope coarse approach. (6) A new, low cost force feedback system. (7) A nanometer-resolution, kHz bandwidth, millimeter range position sensor.

Download or read book Near Field Optics And Nanoscopy written by Jean-pierre Fillard and published by World Scientific. This book was released on 1996-09-20 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contains the most recent information on optical nanoscopy. Far-Field and Near-Field properties on e.m. waves are presented which illustrate how optical images can be obtained from sub-micron objects. Scanning Probe techniques and computer processing are covered here. An explanation is given on how propagating photons or evanescent waves can behave over distances shorter than the wavelength, taking into account the presence of small objects. Quantum tunneling of photons is explained comparatively with the electron mechanism. Technical details are given on photon tunneling microscopes. Typical results already obtained with these techniques are also described.

Download or read book Development of a Near field confocal Polarization Microscope for Local Measurements of Anisotropy in Organic Films written by Andrey Valentinovich Kosterin and published by . This book was released on 2000 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Handbook of Biological Confocal Microscopy written by James Pawley and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 1987 the Electron Microscopy Society of America (EMSA) going to drive important scientific discoveries across wide areas under the leadership of J. P. Revel (Cal Tech) initiated a major of physiology, cellular biology and neurobiology. They had been program to present a discussion of recent advances in light looking for a forum in which they could advance the state of microscopy as part of the annual meeting. The result was three the art of confocal microscopy, alert manufacturers to the lim special LM sessions at the Milwaukee meeting in August 1988: itations of current instruments, and catalyze progress toward The LM Forum, organized by me, and Symposia on Confocal new directions in confocal instrument development. LM, organized by G. Schatten (Madison), and on Integrated These goals were so close to those of the EMSA project that Acoustic/LM/EM organized by C. Rieder (Albany). In addition, the two groups decided to join forces with EMSA to provide there was an optical micro-analysis session emphasizing Raman the organization and the venue for a Confocal Workshop and techniques, organized by the Microbeam Analysis Society, for NSF to provide the financial support for the speakers expenses a total of 40 invited and 30 contributed papers on optical tech and for the publication of extended abstracts.

Download or read book Atomic Force Microscopy Scanning Nearfield Optical Microscopy and Nanoscratching written by Gerd Kaupp and published by Springer Science & Business Media. This book was released on 2006-10-24 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: Making a clear distinction is made between nano- and micro-mechanical testing for physical reasons, this monograph describes the basics and applications of the supermicroscopies AFM and SNOM, and of the nanomechanical testing on rough and technical natural surfaces in the submicron range down to a lateral resolution of a few nm. New or improved instrumentation, new physical laws and unforeseen new applications in all branches of natural sciences (around physics, chemistry, mineralogy, materials science, biology and medicine) and nanotechnology are covered as well as the sources for pitfalls and errors. It outlines the handling of natural and technical samples in relation to those of flat standard samples and emphasizes new special features. Pitfalls and sources of errors are clearly demonstrated as well as their efficient remedy when going from molecularly flat to rough surfaces. The academic or industrial scientist learns how to apply the principles for tackling their scientific or manufacturing tasks that include roughness far away from standard samples.

Download or read book Theory and Practice of Scanning Optical Microscopy written by Tony Wilson and published by . This book was released on 1984 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Laborers in the Vineyard Bible Study written by and published by . This book was released on 2008 with total page 6 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Studies in Confocal Scanning Optical Microscopy written by Timothy Richard Corle and published by . This book was released on 1989 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Development of Optical Microscopy Techniques for the Advancement of Single particle Studies written by and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Single particle orientation and rotational tracking (SPORT) has recently become a powerful optical microscopy tool that can expose many molecular motions. Unfortunately, there is not yet a single microscopy technique that can decipher all particle motions in all environmental conditions, thus there are limitations to current technologies. Within, the two powerful microscopy tools of total internal reflection and interferometry are advanced to determine the position, orientation, and optical properties of metallic nanoparticles in a variety of environments. Total internal reflection is an optical phenomenon that has been applied to microscopy to produce either fluorescent or scattered light. The non-invasive far-field imaging technique is coupled with a near-field illumination scheme that allows for better axial resolution than confocal microscopy and epi-fluorescence microscopy. By controlling the incident illumination angle using total internal reflection fluorescence (TIRF) microscopy, a new type of imaging probe called "non-blinking" quantum dots (NBQDs) were super-localized in the axial direction to sub-10-nm precision. These particles were also used to study the rotational motion of microtubules being propelled by the motor protein kinesin across the substrate surface. The same instrument was modified to function under total internal reflection scattering (TIRS) microscopy to study metallic anisotropic nanoparticles and their dynamic interactions with synthetic lipid bilayers. Utilizing two illumination lasers with opposite polarization directions at wavelengths corresponding to the short and long axis surface plasmon resonance (SPR) of the nanoparticles, both the in-plane and out-of-plane movements of many particles could be tracked simultaneously. When combined with Gaussian point spread function (PSF) fitting for particle super-localization, the binding status and rotational movement could be resolved without degeneracy. TIRS microscopy was also used to find the 3D orientation of stationary metallic anisotropic nanoparticles utilizing only long-axis SPR enhancement. The polarization direction of the illuminating light was rotated causing the relative intensity of p-polarized and s-polarized light within the evanescent field to change. The interaction of the evanescent field with the particles is dependent on the orientation of the particle producing an intensity curve. This curve and the in-plane angle can be compared with simulations to accurately determine the 3D orientation. Differential interference contrast (DIC) microscopy is another non-invasive far-field technique based upon interferometry that does not rely on staining or other contrast enhancing techniques. In addition, high numerical aperture condensers and objectives can be used to give a very narrow depth of field allowing for the optical tomography of samples, which makes it an ideal candidate to study biological systems. DIC microscopy has also proven itself in determining the orientation of gold nanorods in both engineered environments and within cells. Many types of nanoparticles and nanostructures have been synthesized using lithographic techniques on silicon wafer substrates. Traditionally, reflective mode DIC microscopes have been developed and applied to the topographical study of reflective substrates and the imaging of chips on silicon wafers. Herein, a laser-illuminated reflected-mode DIC was developed for studying nanoparticles on reflective surfaces.

Download or read book Near field Nano Atom Optics and Technology written by Motoichi Ohtsu and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Intrinsic features of the optical near field open a new frontier in optical science and technology by finally overcoming the diffraction limit to reach nanometric dimensions. But this book goes beyond near-field optical microscopy to cover local spectroscopy, nanoscale optical processing and storage, quantum near-field optics, and atom manipulation. Near-Field Nano/Atom Optics and Technology provides the first complete and systematically compiled account of the science and technology required to generate the near field, and features applications including imaging of biological specimens and diagnostics for semiconductor nanomaterials and devices. This monograph will be invaluable to researchers who want to implement near-field technology in their own work, and it can also be used as a textbook for graduate or undergraduate students.

Download or read book Handbook of Microscopy for Nanotechnology written by Nan Yao and published by Springer Science & Business Media. This book was released on 2005-03-21 with total page 772 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanostructured materials take on an enormously rich variety of properties and promise exciting new advances in micromechanical, electronic, and magnetic devices as well as in molecular fabrications. The structure-composition-processing-property relationships for these sub 100 nm-sized materials can only be understood by employing an array of modern microscopy and microanalysis tools. Handbook of Microscopy for Nanotechnology aims to provide an overview of the basics and applications of various microscopy techniques for nanotechnology. This handbook highlights various key microcopic techniques and their applications in this fast-growing field. Topics to be covered include the following: scanning near field optical microscopy, confocal optical microscopy, atomic force microscopy, magnetic force microscopy, scanning turning microscopy, high-resolution scanning electron microscopy, orientational imaging microscopy, high-resolution transmission electron microscopy, scanning transmission electron microscopy, environmental transmission electron microscopy, quantitative electron diffraction, Lorentz microscopy, electron holography, 3-D transmission electron microscopy, high-spatial resolution quantitative microanalysis, electron-energy-loss spectroscopy and spectral imaging, focused ion beam, secondary ion microscopy, and field ion microscopy.

Download or read book Optical Near Fields written by Motoichi Ohtsu and published by Springer Science & Business Media. This book was released on 2004-01-22 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book outlines physically intuitive concepts and theories for students, engineers, and scientists who will be engaged in research in nanophotonics and atom photonics. The main topic is the optical near ?eld, i.e., the thin ?lm of light that is localized on the surface of a nanometric material. In the early 1980s, one of the authors (M. Ohtsu) started his pioneering research on optical near ?elds because he judged that nanometer-sized light would be required to shift the paradigm of optical science and technology. This ?eld of research did not exist previously, and was not compatible with trends in opticalscienceandtechnology.However,hewasencouragedbytheknowledge that scientists in other countries started similar research in the mid 1980s. In the 1990s, optical technology progressed very rapidly and the p- tonics industry developed, but further progress became di?cult due to the fundamental limit of light known as the di?raction limit. However, there was a growing awareness among scientists and engineers that this limit can be overcome using optical near ?elds. Since a drastic paradigm shift in the c- cepts of optics is required to understand the intrinsic nature of optical near ?elds, the demand for a textbook on this subject has increased. The present book aims to meet this demand.

Download or read book Principles Of Three dimensional Imaging In Confocal Microscopes written by Min Gu and published by World Scientific. This book was released on 1996-07-29 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses the various principles in confocal scanning microscopy which has become a useful tool in many practical fields including biological studies and industrial inspection. The methodology presented in this book is unique and is based on the concept of the three-dimensional transfer functions which have been developed by the author and his colleagues over the last five years. With the 3-D transfer functions, resolving power in 3-D confocal imaging can be defined in a unified way, different optical arrangements can be compared with an insight into their inter-relationship, and images of thick objects can be modeled in terms of the Fourier transform which makes the analysis easy. The aim of this book is to provide a systematic introduction to the concept of the 3-D transfer functions in various confocal microscopes, to describe the methods for the derivation of different 3-D transfer functions, and to explain the principles of 3-D confocal imaging in terms of these functions.

Download or read book Confocal Microscopy and Multiphoton Excitation Microscopy written by Barry R. Masters and published by SPIE Press. This book was released on 2006 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text guides you through the principles and practical techniques of confocal and multiphoton microscopy. It also describes the historical connections and parallel inventions that resulted in modern techniques of live cell imaging and their use in biology and medicine. You will find comparisons of different types of confocal and multiphoton microscopes, solutions to the problems one would encounter when using various microscopic techniques, tips on selecting equipment, and an extensive annotated bibliography of additional resources.