Download or read book Development and Applications of Novel Fluorescent Molecular Probe Strategies written by Dolonchampa Maji and published by . This book was released on 2017 with total page 149 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical imaging and spectroscopy technologies offer the ability to provide structural and functional information in a fast, low-cost, ionizing radiation free, highly sensitive and high throughput fashion. The diverse contrast mechanisms and complementary imaging platforms form the foundation for the application of optical imaging in pre-clinical studies of pathophysiological development as well as direct clinical application as a tool for diagnosis and therapy. Fluorescence imaging techniques have been one of the most rapidly adopted methods in biology and biomedicine. Visualization of biological processes and pathologic conditions at the cellular and tissue levels largely relies on the use of exogenous fluorophores or their bioconjugates. Some fluorescent molecular probes provide usable contrast for disease diagnosis due to their responsiveness to interactions with other molecular species and/or immediate microenvironment. As a result, understanding exogenous fluorescent contrast mechanisms will allow the development of efficient strategies for biomedical fluorescence imaging. The present work focuses on exploring novel fluorescent molecular probe strategies for imaging cancer and cardiovascular diseases. We have developed a platform for synthesizing activatable fluorescent molecular probes using the fluorescence quenching properties of copper (II) ions. We used these activatable probes for rapid imaging of cancerous tissue in vivo in mice. While developing these molecular probes, we discovered an unexpected molecular interaction that yields stable dimeric molecules. This finding can potentially enable the development of new molecular entities for modifying the signaling properties of fluorescent dyes to minimize background fluorescence. Although planar fluorescence imaging methods using exogenous molecular probes provide rapid information about molecular processes in vivo, extraction of depth information require complex data acquisition and image analysis methods. By designing a dual emission fluorescent probe incorporating two spectrally different fluorophore systems, we developed a method to successfully estimate the depth of fluorescent inclusions from planar imaging data and demonstrated the potential of using this approach to locate a blood vessel and tumorous tissue in mouse in vivo. An important feature of fluorescence methods is the availability of various techniques that provide complementary information. Combining the fluorescence intensity and lifetime properties of a biologically targeted near infrared fluorescent probe, we demonstrate an effective way to distinguish specific from nonspecific uptake mechanisms in cancer cells, an approach that can be translated in vivo. Alternatively, dynamic fluorescence imaging technique expands the scope of applications to include detection and estimation of the size of circulating cancer cells and clusters. The approach developed in this work could allow longitudinal monitoring of these cells, which are implicated in cancer metastases. To circumvent the shallow penetration of light using optical methods, we developed multimodal imaging approaches by incorporating a radionuclide for nuclear imaging into a broad spectrum near infrared fluorescent tumor targeting agent. This molecular construct allows for noninvasive whole body nuclear imaging of tumors, followed by fluorescence image guided resection. In each of these areas, novel fluorescent molecular probes were developed, characterized and applied to solve critical biomedical problems.
Download or read book Development of Fluorescent Molecular Probes for Potential Biological and Materials Applications written by Maura Loew and published by . This book was released on 2014 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Make Life Visible written by Yoshiaki Toyama and published by Springer Nature. This book was released on 2019-10-02 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book describes marked advances in imaging technology that have enabled the visualization of phenomena in ways formerly believed to be completelyimpossible. These technologies have made major contributions to the elucidation of the pathology of diseases as well as to their diagnosis and therapy. The volume presents various studies from molecular imaging to clinical imaging. It also focuses on innovative, creative, advanced research that gives full play to imaging technology inthe broad sense, while exploring cross-disciplinary areas in which individual research fields interact and pursuing the development of new techniques where they fuse together. The book is separated into three parts, the first of which addresses the topic of visualizing and controlling molecules for life. Th e second part is devoted to imaging of disease mechanisms, while the final part comprises studies on the application of imaging technologies to diagnosis and therapy. Th e book contains the proceedings of the 12th Uehara International Symposium 2017, “Make Life Visible” sponsored by the Uehara Memorial Foundation and held from June 12 to 14, 2017. It is written by leading scientists in the field and is an open access publication under a CC BY 4.0 license.
Download or read book Fluorescent Energy Transfer Nucleic Acid Probes written by Vladimir V. Didenko and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. These probes signal detection of their targets by changing either the intensity or the color of their fluorescence. Not surpr- ingly, these luminous, multicolored probes carry more flashy names than their counterparts in the other fields of molecular biology. In recent years, fluor- cent probes and assays, which make use of energy transfer, have multiplied at a high rate and have found numerous applications. However, in spite of this explosive growth in the field, there are no manuals summarizing different p- tocols and fluorescent probe designs. In view of this, the main objective of Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is to provide such a collection. Oligonucleotides with one or several chromophore tags can form fluor- cent probes capable of energy transfer. Energy transport within the probe can occur via the resonance energy transfer mechanism, also called Förster tra- fer, or by non-Förster transfer mechanisms. Although the probes using Förster transfer were developed and used first, the later non-Förster-based probes, such as molecular beacons, now represent an attractive and widely used option. The term “fluorescent energy transfer probes” in the title of this book covers both Förster-based fluorescence resonance energy transfer (FRET) probes and probes using non-FRET mechanisms. Energy transfer probes serve as molecule-size sensors, changing their fluorescence upon detection of various DNA reactions.
Download or read book Development and Application of Novel Fluorescent Probes in Bio sensing written by Vishal Kanthuria and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemosensors written by Binghe Wang and published by John Wiley & Sons. This book was released on 2011-08-24 with total page 830 pages. Available in PDF, EPUB and Kindle. Book excerpt: A thorough, accessible, and general overview of chemosensors Providing a comprehensive overview of chemosensors organic molecules designed to bind and sense small molecules or metal ions and their applications, Chemosensors: Principles, Strategies, and Applications is an accessible one-stop resource for analysts, clinicians, and graduate students studying advanced chemistry and chemosensing. Chemosensors function on a molecular level, generating a signal upon binding. The book reviews their synthesis, design, and applications for detecting biological and organic molecules as well as metal ions. The text highlights applications in drug discovery and catalyses that have not been well covered elsewhere. Covering such topics as molecular recognition, detection methods, design strategies, and important biological issues, the book is broken into four sections that examine intermolecular interactions, strategies in sensor design, detection methods, and case studies in metal, saccharide, and amino acid sensing. An indispensable source of information for chemical and biomedical experts using sensors, Chemosensors includes case studies to make the material both accessible and understandable to chemists of all backgrounds.
Download or read book Fluorescence Spectroscopy Imaging and Probes written by Ruud Kraayenhof and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 401 pages. Available in PDF, EPUB and Kindle. Book excerpt: The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. This topic book, the second in his series, reflects this exciting scientific progress and deals, among others, with new approaches and new probes in fluorescence spectroscopy, single molecule fluorescence, applications in biomembrane and enzyme studies and imaging of living cells.
Download or read book Fluorescence In Situ Hybridization FISH Application Guide written by Thomas Liehr and published by Springer Science & Business Media. This book was released on 2008-11-26 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.
Download or read book Fluorescence Based Biosensors written by and published by Academic Press. This book was released on 2012-12-31 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the major challenges of modern biology and medicine consists in finding means to visualize biomolecules in their natural environment with the greatest level of accuracy, so as to gain insight into their properties and behaviour in a physiological and pathological setting. This has been achieved thanks to the design of novel imaging agents, in particular to fluorescent biosensors. Fluorescence Biosensors comprise a large set of tools which are useful for fundamental purposes as well as for applications in biomedicine, drug discovery and biotechnology. These tools have been designed and engineered thanks to the combined efforts of chemists and biologists over the last decade, and developed hand in hand together with imaging technologies. This volume will convey the many exciting developments the field of fluorescent biosensors and reporters has witnessed over the recent years, from concepts to applications, including chapters on the chemistry of fluorescent probes, on technologies for monitoring protein/protein interactions and technologies for imaging biosensors in cultured cells and in vivo. Other chapters are devoted to specific examples of genetically-encoded reporters, or to protein and peptide biosensors, together with examples illustrating their application to cellular and in vivo imaging, biomedical applications, drug discovery and high throughput screening. Contributions from leading authorities Informs and updates on all the latest developments in the field
Download or read book SMALL MOLECULE BASED FLUORESCENT MOLECULAR PROBES FOR FACILITATING BIOMEDICAL RESEARCH written by and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract : My thesis is focused on the development of fluorescent probes for biosensing and bioimaging within specific organelles. My main research efforts are mainly focused on the design, synthesis and biological applications of these new molecular probes. These new fluorescent probes I developed can be manipulated through the chemical modifications for binding to specific organelles capable of reporting localized bioinformation. Compared to the currently commercially available organelle-specific fluorescent stains, the advantages of the newly synthesized fluorescent probes include low cytotoxicity, high photostability, and long fluorescence lifetimes. These features are crucial for long-time tracking study of biological processes. Research on fluorescent probes with both analyte responsiveness and organelle targetability is a new and emerging area that has attracted increasing attention over the past few years. Because of their high sensitivity, specificity and fast response, these novel fluorescent probes have been proven to be useful tools for facilitating biomedical research. I have further extended the diversity by developing organelle-specific responsive probes capable of detecting changes in biomolecular levels and the microenvironment. My future research efforts give more considerations of the "low-concern" organelles, such as the Golgi apparatus, the endoplasmic reticulum, and ribosomes. Considering the tiny sizes of subcellular organelles, we anticipate that better visualization of the cellular events within specific organelles will rely on super-resolution optical microscopy with nanoscopic-scale resolution.
Download or read book Molecular Fluorescence written by Bernard Valeur and published by John Wiley & Sons. This book was released on 2013-03-25 with total page 595 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.
Download or read book Identification of Biological Species Using Fluorescent Molecular Probes written by Poojitha B Sridhara Setty and published by . This book was released on 2024-05-04 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: According to popular belief, appealing to the sense of sight is more powerful than appealing to the sense of hearing, which could be the origin of the adage ""Seeing is believing."" Technologies that allow researchers to ""see into the body"" or ""see into cells"" are crucial for both basic biological processes research and the detection and treatment of disease. It is ideal for the procedures to be non-invasive, meaning they shouldn't include slicing into the body or separating its components. Thus, in the biomedical sciences, methods for visualising physiological or pathological changes in the body and cells have grown in significance. Contributing to the regulation of numerous physiological processes and playing critical roles in preserving the healthy development of living bodies, a few significant biological species and their microenvironments manage a complex and sensitive dynamic balance in life systems. Living things can develop a variety of illnesses or even pass away if their homeostasis is disturbed. Real-time monitoring of these biological species and their microenvironments during various physiological and pathological processes is therefore extremely important. One of the most effective methods for real-time imaging in biological samples is the use of fluorescent probe-based approaches. Small-molecule fluorescent probes have developed into effective instruments for exploiting light to enhance the field of cell biology research, find novel medications, identify pollutants in the environment, and increase cancer diagnosis. The growth of the fluorescent probe research community-which was tiny in the late 20th century but now comprises over a hundred research groups worldwide-correlates with these uses. It took scientists with a lot of courage from many different professions to join this expansion. In this book, pyrene, coumarin, and naphthalimide are among the fluorescent dyes that are most frequently employed for fluorescence imaging.
Download or read book Development of Two Novel Fluorescent Enzyme Assays written by Andreas Hennig and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This doctoral thesis describes two novel fluorescent methods for assaying enzymatic activity, which are referred to as nanosecond time-resolved fluorescence (Nano-TRF) assays and supramolecular tandem assays. The first method, developed in collaboration with Fa. Hoffmann-La Roche, introduces a novel fluorescent probe (Dbo) for enzyme assays, which combines several desirable properties. First, Dbo has an exceedingly long fluorescence lifetime, which allows the use of Nano-TRF detection to increase the robustness of an assay by suppressing background fluorescence, for example from library compounds in high-throughput screening (HTS). Second, Dbo is efficiently quenched by tryptophan and tyrosine, which allows single-label assays. And third, Dbo has a very small size and hydrophilicity compared to common aromatic hydrophobic fluorescent probes. It has been demonstrated that the combination of these properties affords a minimally invasive, yet very powerful approach to determine the activity of proteases, tyrosine kinases and phosphatases. The second method introduces the use of water-soluble macrocycles and fluorescent dyes for enzyme assays, which presents an economic, convenient, and general assay principle. The assay is based on the competition of dye versus substrate and product in the reversible formation of a complex with the macrocycle. The enzyme thus converts a weak competitor (substrate) into a strong competitor (product) or vice versa, which leads to a different fraction of fluorescent dye bound to the macrocycle. Depending on whether the macrocycle/dye-complex is more or less fluorescent than the free dye, an increase or a decrease in fluorescence results, which signals the enzymatic activity. The method was applied to amino acid decarboxylases and arginase, and the possibility to derive enzyme kinetic parameters and inhibition constants has been demonstrated. Furthermore, the assays were conceptualized by supporting simulation.
Download or read book Fluorescent and Luminescent Probes for Biological Activity written by W. T. Mason and published by Elsevier. This book was released on 1999-04-16 with total page 697 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes. The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research. TOPICS COVERED: * Single and multiphoton confocal microscopy * Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies * Applications of new optical probes for imaging proteins in gels * Probes and detection technologies for imaging membrane potential in live cells * Use of optical probes to detect microorganisms * Raman and confocal raman microspectroscopy * Fluorescence lifetime imaging microscopy * Digital CCD cameras and their application in biological microscopy
Download or read book Fluorescent Energy Transfer Nucleic Acid Probes written by Vladimir V. Didenko and published by Humana. This book was released on 2006-04-01 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. These probes signal detection of their targets by changing either the intensity or the color of their fluorescence. Not surpr- ingly, these luminous, multicolored probes carry more flashy names than their counterparts in the other fields of molecular biology. In recent years, fluor- cent probes and assays, which make use of energy transfer, have multiplied at a high rate and have found numerous applications. However, in spite of this explosive growth in the field, there are no manuals summarizing different p- tocols and fluorescent probe designs. In view of this, the main objective of Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is to provide such a collection. Oligonucleotides with one or several chromophore tags can form fluor- cent probes capable of energy transfer. Energy transport within the probe can occur via the resonance energy transfer mechanism, also called Förster tra- fer, or by non-Förster transfer mechanisms. Although the probes using Förster transfer were developed and used first, the later non-Förster-based probes, such as molecular beacons, now represent an attractive and widely used option. The term “fluorescent energy transfer probes” in the title of this book covers both Förster-based fluorescence resonance energy transfer (FRET) probes and probes using non-FRET mechanisms. Energy transfer probes serve as molecule-size sensors, changing their fluorescence upon detection of various DNA reactions.
Download or read book Molecular Fluorescent Sensors for Cellular Studies written by Elizabeth J. New and published by John Wiley & Sons. This book was released on 2022-08-16 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Fluorescent Sensors for Cellular Studies Enables readers to fully understand the fundamentals and chemical principles of fluorescent sensing and the design of fluorescent sensors Fluorescent sensors are able to provide specific chemical information about cells and can be invaluable in understanding processes that underpin health and disease. Molecular Fluorescent Sensors for Cellular Studies provides an avenue into and overview of currently available fluorescent sensing technology and its application to biological imaging. This book aims to help the reader understand the principles of fluorescence and the mechanisms by which fluorescent sensors operate in order to ensure appropriate and optimal use of sensors. Key applications of fluorescent sensing are presented, with explanations not only of how new sensors can be designed, but also how existing sensors can be applied to various biological settings and conditions. Clear and engaging schematics throughout the book explain chemical principles of sensing to the non-expert. Discusses the breadth of fluorescent sensors, from commercially available sensors to those reported in literature which are yet to be used widely Explains how fluorescent sensors operate for appropriate and optimal use from a theoretical standpoint Provides guidance on how to achieve optimal use of fluorescent sensors in practical settings Summarizes the principles behind fluorescent sensors and their design This work will be an invaluable resource for postgraduates and professionals in the fields of microscopy, bioimaging, and diagnostic imaging who wish to harness the information to improve practical applications and to gain key knowledge surrounding the many facets of fluorescent sensing. It is also of interest to medical and biological researchers working across industry, universities and medical institutes.
Download or read book The Science of Photobiology written by Kendric C. Smith and published by Springer Science & Business Media. This book was released on 2013-03-08 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of The Science of Photobiology was published in 1977, and was the first textbook to cover all of the major areas of photobiology. The science of photobiology is currently divided into 14 subspecialty areas by the American Society for Photobiology. In this edition, however, the topics of phototechnology and spectroscopy have been com bined in a new chapter entitled "Photophysics." The other subspecialty areas remain the same, i.e., Photochemistry, Photosensitization, UV Radiation Effects, Environmental Photobiology, Photomedicine, Circadian Rhythms, Extraretinal Photoreception, Vision, Photomorphogenesis, Photomovement, Photosynthesis, and Bioluminescence. This book has been written as a textbook to introduce the science of photobiology to advanced undergraduate and graduate students. The chapters are written to provide a broad overview of each topic. They are designed to contain the amount of information that might be presented in a one-to two-hour general lecture. The references are not meant to be exhaustive, but key references are included to give students an entry into the literature. Frequently a more recent reference that reviews the literature will be cited rather than the first paper by the author making the original discovery. The chapters are not meant to be a repository of facts for research workers in the field, but rather are concerned with demon strating the importance of each specialty area of photobiology, and documenting its relevance to current and/or future problems of man.
