Download or read book Developing Gene Delivery Vehicles Using Self assembly written by Chung-Huei Katherine Wang and published by . This book was released on 2011 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Self Assembling Complexes for Gene Delivery written by Alexander V. Kabanov and published by Wiley-Blackwell. This book was released on 1998-05-29 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent years have witnessed an explosion of activity in the field of gene therapy. Following advances in our understanding of the molecular basis of disease, hopes are high that the tremendous potential market for drugs employing antisense nucleotides and genes will one day be fulfilled. However, many obstacles remain, not least problems in the technology of gene delivery systems. Much of today's research focuses on non-viral approaches to gene delivery. Of particular importance are supramolecular complexes formed between DNA and various natural and synthetic polymers and lipids, otherwise known as 'self-assembling complexes for gene delivery'. In view of this fact, the editors of this volume have assembled an international team of contributors to present up-to-date reviews of the major chemical, biological and clinical aspects of such gene delivery vectors. Reflecting the diversity of research in this field, this book comprehensively covers: * the principles of self-assembly * natural mechanisms for gene delivery to cells * cationic lipids and liposomes * polyelectrolyte DNA complexes * systemic biodistribution of drug delivery systems * targeting of conjugates for gene delivery * new approaches to gene delivery * clinical evaluation Self-assembling Complexes for Gene Delivery is an essential reference for all professionals with an interest in gene and antisense therapy or novel drug delivery systems, including medicinal and pharmaceutical chemists, clinicians, human geneticists, molecular biologists and pharmacologists.

Download or read book Synthetic DNA Delivery Systems written by Dan Luo and published by Springer Science & Business Media. This book was released on 2003-09-30 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA delivery into cells is a rapidly developing area in gene therapy and biotechnology. Moreover, it is a powerful research tool to determine gene structure, regulation, and function. Viral methods of DNA delivery are well-characterized and efficient, but little is known about the toxicity and immunogenecity of viral vectors. As a result, non-viral, transfection methods of DNA delivery are of increasing interest. Synthetic DNA Delivery Systems is a comprehensive and current resource on DNA transfection. The use of histidine-rich peptides and polypeptides as DNA delivery systems and self-assembled delivery systems based on cationic lipids and polymers are discussed. Targeted delivery to organelles, tumor cells and dendritic cells comprise an important topic.

Download or read book Viral Nanoparticles written by Nicole F. Steinmetz and published by CRC Press. This book was released on 2019-08-21 with total page 281 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book overviews the applications of viral nanoparticles (VNPs) in areas ranging from materials science to biomedicine. It summarizes the many different VNP building blocks and describes chemistries that allow one to attach, entrap, or display functionalities on VNPs. The book outlines the strategies for the construction of 1-, 2-, and 3-D arrays, highlights the achievements in utilizing VNPs as tools for novel biosensors and nanoelectronic devices, and describes efforts in designing VNPs for biomedical applications, including their use as gene delivery vectors, novel vaccines, imaging modalities, and applications in targeted therapeutics.

Download or read book Non viral Gene Delivery Vectors Based on New Unsymmetrical Bolaamphiphiles written by Namrata Jain and published by . This book was released on 2010 with total page 209 pages. Available in PDF, EPUB and Kindle. Book excerpt: The success in gene therapy relies strongly on new efficient gene delivery vectors. Non-viral vectors are highly attractive, since they can deliver large quantities of genetic information and are low immunogenic. In this respect, unsymmetrical bolaamphiphiles (bolas) bearing two different hydrophilic head-groups connected by a hydrophobic spacer could be an attractive alternative for vector design, as they can form well-defined supramolecular nanostructures (nanovesicles and nanotubes) that are more stable than those formed with lipids. The aim of the present work was to develop new gene delivery vectors based on bolas capable to form such nanostructures hosting a DNA molecule and exposing at their external surface neutral ligands. For this purpose, a variety of bolas, bearing cationic and neutral groups connected by a hydrophobic spacer, were obtained by multi-step organic synthesis. Their characterization by different instrumental techniques suggested that the nature of the head groups as well as the hydrophobic spacer defines the bola self-assembly, their interaction with DNA and the morphology of their complexes (bolaplexes). While the first two generations of bolas lack the essential features of nonviral vectors, the final third generation was found highly promising. The latter showed a strong interaction with DNA, the formation of small bolaplexes and good transfection efficiency in different cell lines. The increase in transfection efficiency in presence of DOPE or chloroquine suggested that the key barrier for their internalization could be the lipid fusion and the endosomal escape. Some bolaplex formulations showed a transfection efficiency comparable to the best commercial transfection agents. Thus, the present work introduces a new class of efficient transfection agents based on unsymmetrical bolaamphiphiles.

Download or read book Development of a Tailorable and Tunable Mechanism for Cell responsive Substrate mediated Gene Delivery written by Kory M. Blocker and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to the spatial and temporal control as well as the cell-type specificity necessary to extend gene delivery to therapeutic applications, there exists a need to create systems capable of gene transfer that are well-understood and easily manipulated. Furthermore, the creation of such materials will enable further exploration of the correlation between biochemical cues and the resulting cellular responses. In response to this as yet unmet need, a method to promote cell-responsive substrate-mediated gene delivery was developed for this dissertation. Through the use of non-viral gene delivery, flexibility of the vehicle design was incorporated into the system. Using PNA technology, pDNA was able to be specifically tethered to a self-assembled monolayer via an enzymatically-labile peptide tether. This construct was shown to promote cell-responsive delivery while retaining flexibility over the chemical and physical properties of the vehicle and substrate. By alteration of some design parameters including tether number, pDNA surface coverage, and complexation agent, temporal control over the release profile was demonstrated. Furthermore, the ability to extend the applicability of the system was detailed by transitioning to a poly-D-lysine coated substrate upon which the pDNA is immobilized. This dissertation details proof-of-principle work in the formation of a controlled release gene delivery mechanism that may be used to promote understanding of cellular responses to biochemical signaling as well as be extended to use in tissue engineering applications.

Download or read book The New Physics written by Gordon Fraser and published by Cambridge University Press. This book was released on 2006-02-13 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: Underpinning all the other branches of science, physics affects the way we live our lives, and ultimately how life itself functions. Recent scientific advances have led to dramatic reassessment of our understanding of the world around us, and made a significant impact on our lifestyle. In this book, leading international experts, including Nobel prize winners, explore the frontiers of modern physics, from the particles inside an atom to the stars that make up a galaxy, from nano-engineering and brain research to high-speed data networks. Revealing how physics plays a vital role in what we see around us, this book will fascinate scientists of all disciplines, and anyone wanting to know more about the world of physics today.

Download or read book Gene Transfer in the Cardiovascular System written by Keith L. March and published by Springer. This book was released on with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goal of gene transfer is protein expression. a process brought about by the insertion of a gene coding for a foreign protein into target cells resulting in the synthesis of the foreign protein For gene therapy, a tmnsferred therapeutic gene must be expressed at a level beneficial for the patient. This chapter provides an introductory overview of the rapidly evolving field of non-viral approaches for gene delivery to rnarnrnalian cells. Although currently there are fewer ongoing clinical trials using non-viral approaches than those using viral based systems, the number of non-viral trials is increasing. The long range goal of some research groups is the development of a genetically engineered artificial virus targeted to specific cells in the human body. An arurual conference, organized by Cambridge Healthtech Institute entitled "Artificial Self-Assembling Systems for Gene Transfer", brings together researchers interested in this field [1]. Assembly of an artificial virus is very complex; other research groups aim to develop simpler delivery systems consisting of a plasmid combined with delivery agents. Viral-based systems are very successful for gene delivery, but despite their successes, viral-based systems have some geneml limitations and system-specific limitations. When employing a viml-based system, the following limitations should be considered: • size limitation of the inserted gene due to packaging constraints (e. g. adenovirus, retrovirus) . • potential tumorigenesis (e. g. retrovirus) • potential for insertional mutagenesis (greater than plasmid based systems) • potential imrnunogenicity (e. g.

Download or read book Intracellular Delivery III written by Aleš Prokop and published by Springer. This book was released on 2016-10-31 with total page 455 pages. Available in PDF, EPUB and Kindle. Book excerpt: A critical review is attempted to assess the status of nanomedicine entry onto the market. The emergence of new potential therapeutic entities such as DNA and RNA fragments requires that these new “drugs” will need to be delivered in a cell-and organelle-specific manner. Although efforts have been made over the last 50 years or so to develop such delivery technology, no effective and above all clinically approved protocol for cell-specific drug delivery in humans exists as yet. Various particles, macromolecules, liposomes and most recently “nanomaterials” have been said to “show promise” but none of these promises have so far been “reduced” to human clinical practice. The focus of this volume is on cancer indication since the majority of published research relates to this application; within that, we focus on solid tumors (solid malignancies). Our aim is critically to evaluate whether nanomaterials, both non-targeted and targeted to specific cells, could be of therapeutic benefit in clinical practice. The emphasis of this volume will be on pharmacokinetics (PK) and pharmacodynamics (PD) in animal and human studies. Apart from the case of exquisitely specific antibody-based drugs, the development of target-specific drug–carrier delivery systems has not yet been broadly successful at the clinical level. It can be argued that drugs generated using the conventional means of drug development (i.e., relying on facile biodistribution and activity after (preferably) oral administration) are not suitable for a target-specific delivery and would not benefit from such delivery even when a seemingly perfect delivery system is available. Therefore, successful development of site-selective drug delivery systems will need to include not only the development of suitable carriers, but also the development of drug entities that meet the required PK/PD profile.

Download or read book Fields Virology Emerging Viruses written by Peter M. Howley and published by Lippincott Williams & Wilkins. This book was released on 2020-02-11 with total page 2597 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now in four convenient volumes, Field’s Virology remains the most authoritative reference in this fast-changing field, providing definitive coverage of virology, including virus biology as well as replication and medical aspects of specific virus families. This volume of Field’s Virology: Emerging Viruses, 7th Edition covers recent changes in emerging viruses, providing new or extensively revised chapters that reflect these advances in this dynamic field.

Download or read book Synthesis and Applications of Inorganic Nanostructures written by Huaqiang Cao and published by John Wiley & Sons. This book was released on 2017-09-13 with total page 561 pages. Available in PDF, EPUB and Kindle. Book excerpt: Authored by a leading figure in the field, this book systematically describes all the fundamental aspects and applications of inorganic nanostructures from zero to three dimensions. It not only discusses various synthesis technologies, but also covers the physical properties of inorganic nanostructures, such as optical, electric and magnetic properties, and practical applications such as energy storage (including Li-ion and Ni-MH batteries and supercapacitors), superhydrophobic and bio-applications, etc. The focus throughout is on the synthesis-structure-application relationships, including the growth mechanisms for the nanostrucutres. Concise yet comprehensive, this is indispensable reading for chemists and materials scientists.

Download or read book Developing Novel Drug Delivery Systems For Platinum based Anticancer Drugs Using Coordination driven Self assembly written by Han Wang and published by . This book was released on 2020 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Platinum complexes are the most common anticancer agents used in the chemotherapeutic treatment regimens. (Wheate, Walker et al. 2010) Cisplatin, carboplatin, and oxaliplatin are the FDA-approved platinum-based chemotherapeutics used for clinical cancer therapy. (Wang 2005) However, patients treated with Pt-based drugs usually suffer from a variety of side effects including kidney damage, nerve damage, hearing loss, and suppression of bone marrow activity. To overcome these undesired side effects, it is necessary to develop a suitable drug delivery system for carrying anticancer agents to the target location. (Farokhzad and Langer 2009) The primary goal of this work is to combine the Pt (IV) prodrugs with nanodelivery to develop new drug delivery systems. In the second chapter, we present a novel design to use metal-organic cages (MOCs) to encapsulate Pt-based anticancer agents for delivery. A fluorescein conjugated Pt (IV) prodrug of cisplatin is developed for its encapsulation in a cationic MOC via host-guest interactions, which then forms drug-loaded nanoparticles with an anionic polymer. In Chapter 3, we design a Pt6L4 hydrophobic metallacage to encapsulate combat platinum resistance in ovarian cancer cells. The cage is further formulated into nanoparticles upon mixing with fluorescein and pegylated anionic polymer. The metal organic cage has a 20-fold increase in solubility and exhibits lower resistance factor on chemoresistance ovarian cancer cell lines. In Chapter 4, we develop a new strategy to conjugate Pt (IV) prodrugs covalently to self-assembled supramolecular hexagon. This new platinum drug delivery platform controls the drug loading precisely and displays a superior therapeutic index compared to cisplatin against a panel of human cancer cell lines. In Chapter 5, we demonstrate two kinds of self-assembled M12L24 metallosupramolecular spheres which can further form lipid-coated nanoparticles. Such novel nanoparticles exhibit superior therapeutic effects compared with that of cisplatin and trigger both DNA and mitochondria damage after entering cancer cells. References Farokhzad, O. C. and R. Langer (2009). "Impact of nanotechnology on drug delivery." ACS nano 3(1): 16-20.Wang, D. (2005). "Lippard sJ." Cellular processing of platinum anticancer drugs. Nat Rev Drug Discov 4: 307-320.Wheate, N. J., et al. (2010). "The status of platinum anticancer drugs in the clinic and in clinical trials." Dalton transactions 39(35): 8113-8127.

Download or read book Preclinical Development Handbook written by Shayne Cox Gad and published by John Wiley & Sons. This book was released on 2008-03-21 with total page 1352 pages. Available in PDF, EPUB and Kindle. Book excerpt: A clear, straightforward resource to guide you through preclinical drug development Following this book's step-by-step guidance, you can successfully initiate and complete critical phases of preclinical drug development. The book serves as a basic, comprehensive reference to prioritizing and optimizing leads, dose formulation, ADME, pharmacokinetics, modeling, and regulations. This authoritative, easy-to-use resource covers all the issues that need to be considered and provides detailed instructions for current methods and techniques. Each chapter is written by one or more leading experts in the field. These authors, representing the many disciplines involved in preclinical toxicology screening and testing, give you the tools needed to apply an effective multidisciplinary approach. The editor has carefully reviewed all the chapters to ensure that each one is thorough, accurate, and clear. Among the key topics covered are: * Modeling and informatics in drug design * Bioanalytical chemistry * Absorption of drugs after oral administration * Transporter interactions in the ADME pathway of drugs * Metabolism kinetics * Mechanisms and consequences of drug-drug interactions Each chapter offers a full exploration of problems that may be encountered and their solutions. The authors also set forth the limitations of various methods and techniques used in determining the safety and efficacy of a drug during the preclinical stage. This publication should be readily accessible to all pharmaceutical scientists involved in preclinical testing, enabling them to perform and document preclinical safety tests to meet all FDA requirements before clinical trials may begin.

Download or read book Design Principle and Application of Self Assembled Nanobiomaterials in Biology and Medicine written by Alok Pandya and published by Academic Press. This book was released on 2022-08-04 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: Design, Principle and Application of Self-Assembled Nanobiomaterials in Biology and Medicine discusses recent advances in science and technology using nanoscale units that show the novel concept of combining nanotechnology with various research disciplines within both the biomedical and medicine fields. Self-assembly of molecules, macromolecules, and polymers is a fascinating strategy for the construction of various desired nanofabrication in chemistry, biology, and medicine for advanced applications. It has a number of advantages: (1) It is involving atomic-level modification of molecular structure using bond formation advanced techniques of synthetic chemistry. (2) It draws from the enormous wealth of examples in biology for the development of complex, functional structures. (3) It can incorporate biological structures directly as components in the final systems. (4) It requires that the target self-assembled structures be thermodynamically most stable with relatively defect-free and self-healing. In this book, we cover the various emerging self-assembled nanostructured objects including molecular machines, nano-cars molecular rotors, nanoparticles, nanosheets, nanotubes, nanowires, nano-flakes, nano-cubes, nano-disks, nanorings, DNA origami, transmembrane channels, and vesicles. These self-assembled materials are used for sensing, drug delivery, molecular recognition, tissue engineering energy generation, and molecular tuning. Provides a basic understanding of how to design, and implement various self-assembled nanobiomaterials Covers principles implemented in the constructions of novel nanostructured materials Offers many applications of self-assemblies in fluorescent biological labels, drug and gene delivery, bio-detection of pathogens, detection of proteins, probing of DNA structure, tissue engineering, and many more

Download or read book Polymeric Gene Delivery Systems written by Yiyun Cheng and published by Springer. This book was released on 2018-09-04 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: ​The series Topics in Current Chemistry Collections presents critical reviews from the journal Topics in Current Chemistry organized in topical volumes. The scope of coverage is all areas of chemical science including the interfaces with related disciplines such as biology, medicine and materials science. The goal of each thematic volume is to give the non-specialist reader, whether in academia or industry, a comprehensive insight into an area where new research is emerging which is of interest to a larger scientific audience. Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field. The chapter "Polymeric Nanoparticle-Mediated Gene Delivery for Lung Cancer Treatment" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Download or read book Polymeric Nano Biomaterials for Medical Applications Advancements in Developing and Implementation Considering Safety By Design Concepts written by Gerrit Borchard and published by Frontiers Media SA. This book was released on 2020-12-22 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.

Download or read book Gene Therapy Methods written by M. Ian Phillips and published by Academic Press. This book was released on 2002-02-22 with total page 770 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume in the prestigious Methods in Enzymology series discusses methods currently used in preclinical and clinical gene therapy. Subjects covered in this book, such as the use of adeno-associated virus delivery for treatment of Parkinson's disease, are topical and are presented in the methods-oriented style popularized by this series. Discusses methods currently used in preclinical and clinical gene therapy Covers the use of adeno-associated virus delivery for treatment of Parkinson's disease