Download or read book Developing Droplet Microfluidic Technologies for Single cell and Single molecule Assays written by Soroush Kahkeshani and published by . This book was released on 2017 with total page 115 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past decade, growing number of commercialized "lab on a chip" systems have been addressing needs in biology for patients and researchers. The ability to miniaturize conventional systems used in lab while increasing sensitivity in addition to newly developed platforms, have enabled new applications such as point-of-care diagnostics and single-cell sequencing. This dissertation focuses on new droplet microfluidic platforms developed for addressing the current challenges (controlling encapsulation beyond Poisson distribution, removing bulky and expensive pumps,...) in commercialization of single-cell and single-molecule assays. In chapter 1, current state of the art, recent commercialized products and their limitations are discussed. In chapter 2, new findings about particle interactions in single-phase microfluidics leading to "preferred interparticle spacings" and trajectory attractors in inertial microflow regimes are presented. Furthermore, these findings have been combined with new modifications made to conventional flow focusing droplet generators in order to avoid jetting regime in inertial regimes, thus allowing making use of preferred spacing between cells/particles in inertial flows for controlling number of cells/particles encapsulated in droplets (one cell/particle per drop). Our findings could be used for many applications such as drug discovery. The other application could be for droplet sequencing (drop-seq) where for example RNA of individual cells are sequenced and it is important that only one cell is present in each droplet to generate unbiased data. In chapter 3 and 4 of the dissertation, new patented technology for generation of droplets, replacing bulky and expensive pumps, with body force exerted on the ferrofluid by magnetic field acting on magnetic nanoparticles mixed with the fluid. Thus the cost and footprint of the device is reduced significantly, allowing for use in limited-resource settings (global health) for quantification of biomolecules by controlled magnetic dosing of nanoliter droplets.

Download or read book Functional Metagenomics Tools and Applications written by Trevor C. Charles and published by Springer. This book was released on 2017-10-09 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book, the latest tools available for functional metagenomics research are described.This research enables scientists to directly access the genomes from diverse microbial genomes at one time and study these “metagenomes”. Using the modern tools of genome sequencing and cloning, researchers have now been able to harness this astounding metagenomic diversity to understand and exploit the diverse functions of microorganisms. Leading scientists from around the world demonstrate how these approaches have been applied in many different settings, including aquatic and terrestrial habitats, microbiomes, and many more environments. This is a highly informative and carefully presented book, providing microbiologists with a summary of the latest functional metagenomics literature on all specific habitats.

Download or read book Microfluidics for Single Cell Analysis written by Jin-Ming Lin and published by Springer Nature. This book was released on 2019-08-28 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.

Download or read book Microfluidics and Nanotechnology written by Eric Lagally and published by CRC Press. This book was released on 2017-12-19 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: An increasing number of technologies are being used to detect minute quantities of biomolecules and cells. However, it can be difficult to determine which technologies show the most promise for high-sensitivity and low-limit detection in different applications. Microfluidics and Nanotechnology: Biosensing to the Single Molecule Limit details proven approaches for the detection of single cells and even single molecules—approaches employed by the world’s foremost microfluidics and nanotechnology laboratories. While similar books concentrate only on microfluidics or nanotechnology, this book focuses on the combination of soft materials (elastomers and other polymers) with hard materials (semiconductors, metals, and glass) to form integrated detection systems for biological and chemical targets. It explores physical and chemical—as well as contact and noncontact—detection methods, using case studies to demonstrate system capabilities. Presenting a snapshot of the current state of the art, the text: Explains the theory behind different detection techniques, from mechanical resonators for detecting cell density to fiber-optic methods for detecting DNA hybridization, and beyond Examines microfluidic advances, including droplet microfluidics, digital microfluidics for manipulating droplets on the microscale, and more Highlights an array of technologies to allow for a comparison of the fundamental advantages and challenges of each, as well as an appreciation of the power of leveraging scalability and integration to achieve sensitivity at low cost Microfluidics and Nanotechnology: Biosensing to the Single Molecule Limit not only serves as a quick reference for the latest achievements in biochemical detection at the single-cell and single-molecule levels, but also provides researchers with inspiration for further innovation and expansion of the field.

Download or read book Microfluidic Methods for Molecular Biology written by Chang Lu and published by Springer. This book was released on 2016-05-14 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and forensics, and microfluidic in situ hybridization for RNA analysis. Key molecular biology studies using model organisms are reviewed in detail. This is an ideal book for students and researchers in the microfluidics and molecular biology fields as well as engineers working in the biotechnology industry. This book also: Reviews exhaustively the latest techniques for single-cell genetic, epigenetic, metabolomic, and proteomic analysis Illustrates microfluidic approaches for inverse metabolic engineering, as well as analysis of circulating exosomes Broadens readers’ understanding of microfluidics convection-based PCR technology, microfluidic RNA-seq, and microfluidics for robust mobile diagnostics

Download or read book Single Cell Assays written by Paul C.H. Li and published by Springer Nature. This book was released on 2023-07-10 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores the use of single-cell assays in research for drug discovery, microfluidics, and more. The book delves into methodologies involving a variety of cell types and diseases, small molecules and biologics, as well as studies of the genome and transciptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Single-Cell Assays: Microfluidics, Genomics, and Drug Discovery serves to enable researchers to obtain a rapid overview in state-of-the-art microfluidic single-cell assays and an impression of what possibilities these assays offer to drug discovery.

Download or read book Microfluidics and Nanotechnology written by Eric Lagally and published by . This book was released on 2014-01-01 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: An increasing number of technologies are being used to detect minute quantities of biomolecules and cells. However, it can be difficult to determine which technologies show the most promise for high-sensitivity and low-limit detection in different applications. Microfluidics and Nanotechnology: Biosensing to the Single Molecule Limit details proven approaches for the detection of single cells and even single molecules approaches employed by the world s foremost microfluidics and nanotechnology laboratories. While similar books concentrate only on microfluidics or nanotechnology, this book focuses on the combination of soft materials (elastomers and other polymers) with hard materials (semiconductors, metals, and glass) to form integrated detection systems for biological and chemical targets. It explores physical and chemical as well as contact and noncontact detection methods, using case studies to demonstrate system capabilities. Presenting a snapshot of the current state of the art, the text: Explains the theory behind different detection techniques, from mechanical resonators for detecting cell density to fiber-optic methods for detecting DNA hybridization, and beyond Examines microfluidic advances, including droplet microfluidics, digital microfluidics for manipulating droplets on the microscale, and more Highlights an array of technologies to allow for a comparison of the fundamental advantages and challenges of each, as well as an appreciation of the power of leveraging scalability and integration to achieve sensitivity at low cost Microfluidics and Nanotechnology: Biosensing to the Single Molecule Limit not onlyserves as a quick reference for the latest achievements in biochemical detection at the single-cell and single-molecule levels, but also provides researchers with inspiration for further innovation and expansion of the field."

Download or read book Cell Analysis on Microfluidics written by Jin-Ming Lin and published by Springer. This book was released on 2017-10-25 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a detailed overview of the design, formatting, application, and development of microfluidic chips in the context of cell biology research, enumerating each element involved in microfluidics-based cell analysis, discussing its history, status quo, and future prospects, It also offers an extensive review of the research completed in the past decade, including numerous color figures. The individual chapters are based on the respective authors' studies and experiences, providing tips from the frontline to help researchers overcome bottlenecks in their own work. It highlights a number of cutting-edge techniques, such as 3D cell culture, microfluidic droplet technique, and microfluidic chip-mass spectrometry interfaces, offering a first-hand impression of the latest trends in the field and suggesting new research directions. Serving as both an elementary introduction and advanced guidebook, the book interests and inspires scholars and students who are currently studying microfluidics-based cell analysis methods as well as those who wish to do so.

Download or read book Droplet Microfluidics written by Eric Brouzes and published by . This book was released on 2021 with total page 114 pages. Available in PDF, EPUB and Kindle. Book excerpt: Droplet microfluidics has dramatically developed in the past decade and has been established as a microfluidic technology that can translate into commercial products. Its rapid development and adoption have relied not only on an efficient stabilizing system (oil and surfactant), but also on a library of modules that can manipulate droplets at a high-throughput. Droplet microfluidics is a vibrant field that keeps evolving, with advances that span technology development and applications. Recent examples include innovative methods to generate droplets, to perform single-cell encapsulation, magnetic extraction, or sorting at an even higher throughput. The trend consists of improving parameters such as robustness, throughput, or ease of use. These developments rely on a firm understanding of the physics and chemistry involved in hydrodynamic flow at a small scale. Finally, droplet microfluidics has played a pivotal role in biological applications, such as single-cell genomics or high-throughput microbial screening, and chemical applications. This Special Issue will showcase all aspects of the exciting field of droplet microfluidics, including, but not limited to, technology development, applications, and open-source systems.

Download or read book Unravelling Single Cell Genomics written by Nathalie Bontoux and published by Royal Society of Chemistry. This book was released on 2010-10-18 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique introduction to the growing field of microfluidics applied to genomics provides an overview of the latest technologies and emphasizes its potential in answering important biological questions. Written by a physicist and a biologist, it offers a more comprehensive view than the previous literature. The book starts with key ideas in molecular biology, developmental biology and microtechnology before going on to cover the specifics of single cell analysis and microfluidic devices for single cell molecular analysis. Review chapters discuss the state-of-the art and will prove invaluable to all those planning to develop microdevices for molecular analysis of single cells. Methods allowing complete analysis of gene expression in the single cell are stressed - as opposed the more commonly used techniques that allow analysis of only a few genes at a time. As pioneers in the field, the authors understand how critical it is for a physicist to understand the biological issues and questions related to single cell analysis, as well for biologists to understand what microfluidics is all about. Aimed predominantly at graduate students, this book will also be of significant interest to scientists working in or affiliated with this field.

Download or read book High Throughput Microfluidic Technologies for Cell Separation and Single cell Analysis written by Lidan Wu (Ph. D.) and published by . This book was released on 2016 with total page 102 pages. Available in PDF, EPUB and Kindle. Book excerpt: The heterogeneity of individual cellular behavior in response to physical and chemical stimuli has raised increasing attention in many biological processes. There is great incentive in developing techniques for high throughput single-cell measurements and manipulations. Particularly, cell size has been recognized as an important parameter in single cell study and pericellular protease activity plays a key role in regulating the microenvironment of individual cells. Therefore, this thesis focuses on establishing new methods to address the issues of cell size and single cell protease measurement. We first develop a size-based cell separation technique using Dean-coupled inertial microfluidic sorter. Separation of cells by size before downstream assays might be beneficial in simplifying the system and facilitating the discovery of rare subpopulations through enrichment of cells with certain size range or cell cycle phase. By investigating the particle focusing and separation mechanisms in curved microfluidic channel, we develop a novel design of inertial microfluidic sorter with higher separation resolution and then demonstrate its capacity in leukocyte isolation from blood. This novel cell sorter would be a promising alternative to many other cell separation problems. We then establish a microfluidic platform for functional measurement of single cell pericellular proteases, including both those secreted and expressed on cell surface. We apply the platform to studying the PMA-mediated protease response of HepG2 cells at single-cell level and reveal the diversity in the dynamic patterns of single-cell protease activity profile upon drug stimulation. We also present the preliminary exploration of single-cell protease activity behavior in anticancer drug resistance development. Lastly, we explore the applicability of our platform for single-cell shedding measurement. Protease-mediated molecular shedding is one of the key mechanisms through which individual cells actively regulate their own microenvironment. However, the amount of molecules being shed for individual cells is extremely low, posing significant challenges in detecting shedding quantitatively. By means of analytical analysis and numerical simulations, we investigate the intrinsic noise of low-abundance molecule detection. Experimental characterizations have also been performed to evaluate the impact of practical factors on actual readout variation.

Download or read book Applications of Microfluidic Systems in Biology and Medicine written by Manabu Tokeshi and published by Springer Nature. This book was released on with total page 559 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Microfluidics in Cell Biology Part C Microfluidics for Cellular and Subcellular Analysis written by and published by Academic Press. This book was released on 2018-11-22 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics in Cell Biology Part C, Volume 148, a new release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various multi-cellular models, including microfluidics in cell monolayers/spheroids, microfluidics in organ on chips, and microfluidics in model organisms. Specific chapters discuss collective migration in microtubes, leukocyte adhesion dynamics on endothelial monolayers under flow, constrained spheroid for perfusion culture, cells in droplet arrays, heart on chips, kidney on chips, liver on chips, and more. - Contains contributions from experts in the field from across the world - Covers a wide array of topics on both mitosis and meiosis - Includes relevant, analysis based topics

Download or read book Microdroplet Technology written by Philip Day and published by Springer Science & Business Media. This book was released on 2012-07-28 with total page 249 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microdroplet technology has recently emerged to provide new and diverse applications via microfluidic functionality, especially in various areas of biology and chemistry. This book, then, gives an overview of the principle components and wide-ranging applications for state-of-the-art of droplet-based microfluidics. Chapter authors are internationally-leading researchers from chemistry, biology, physics and engineering that present various key aspects of micrdroplet technology -- fundamental flow physics, methodology and components for flow control, applications in biology and chemistry, and a discussion of future perspectives. This book acts as a reference for academics, post-graduate students, and researcher wishing to deepen their understand of microfluidics and introduce optimal design and operation of new droplet-based microfluidic devices for more comprehensive analyte assessments.

Download or read book Novel Droplet Techniques for Integrative Single Cell Analyses written by Kara Kimberly Brower and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The last decade has yielded transformative new methods to analyze single cells, revealing new insights into cellular diversity, tissue organization, and organism development. Droplet microfluidics, in particular, has made it possible to characterize single cells by their genome, epigenome, and transcriptome at unprecedented scale. These techniques have given rise to the first cell atlases, catalogs of hundreds of thousands of cells classified by type across tissues, systems, and even whole organisms. However, despite these advances, researchers lack an unbiased, high-throughput means to link cellular phenotype to genotype within the same cell. This capability would allow single cell analyses to move beyond simply characterizing the heterogeneity within systems and toward a more mechanistic understanding of whole-cell regulation. Here I describe the development of a new technology, Droception, that marries the three most powerful single cell analysis techniques -- droplet microfluidics, flow cytometry, and next-generation sequencing -- to screen and sort cells by complex phenotypes with downstream sequencing. This technology has the potential to expand our mechanistic understanding of rare biological populations, identify novel cell types unresolved by current techniques, and unlock new avenues for single cell multi-omics.

Download or read book ImmunoPhysics and ImmunoEngineering written by Jorge Bernardino De La Serna and published by Frontiers Media SA. This book was released on 2020-03-30 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: ImmunoPhysics (ImmPhys) and ImmunoEngineering (ImmPhysEng), are two cross-disciplinary fields. ImmPhysEng aims to unravel quantitatively the immune-system function and regulation in health and disease. Whereas ImmPhys study and assess the physical basis of the immune response, ImmEng pursues its control and prediction. Ultimately, the overarching goal of these disciplines is to facilitate the development of therapeutic interventions to more precisely modulate and control the compromised immune response during diseases. Lately, these disciplines are becoming more popular and as such, the number of publications applying physical or engineering tools to understand the immune response is increasing. Nevertheless, there is still no scientific forum compiling the ImmPhysEng research breakthroughs. Possibly the biggest burden is to stimulate a fluent communication and syntony between a physicist or engineer and an immunologist.

Download or read book 7th International Conference on the Development of Biomedical Engineering in Vietnam BME7 written by Vo Van Toi and published by Springer. This book was released on 2019-06-05 with total page 673 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents the proceedings of the 7th International Conference on the Development of Biomedical Engineering in Vietnam which was held from June 27-29, 2018 in Ho Chi Minh City. The volume reflects the progress of Biomedical Engineering and discusses problems and solutions. It aims to identify new challenges, and shaping future directions for research in biomedical engineering fields including medical instrumentation, bioinformatics, biomechanics, medical imaging, drug delivery therapy, regenerative medicine and entrepreneurship in medical devices.