Download or read book Determination of Degradative Gene Frequencies written by Karen Lynn Mumy and published by . This book was released on 2004 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aerobic polycyclic aromatic hydrocarbon (PAH) degradative pathways have been described in a wide variety of bacteria, fungi and algae. The occurrence and role of these pathways and their corresponding genes in natural attenuation in environmental systems remain poorly understood. The primary goal of this dissertation research was to determine the concentration and frequency of five degradative genes within natural microbial communities exposed to ambient, low and high levels of PAHs using a quantitive-competitive PCR approach adapted for microbial ecology use. PAH concentrations, total microbial biomass and microbial community structure for sediment samples were also determined using a coupled biochemical approach. Community structure analysis indicated that microeukaryotes may be indirectly responding to the contamination, suggesting the development of a PAH-based food web within the microbial communities. Gene frequencies and concentrations reflected increases with elevated levels of contamination. However, the measured frequencies for most genes indicated that only 0.1% or less of the microbial community had the genetic capacity to degrade the contaminants using described aerobic pathways and their corresponding genes and suggested these sequences are rare in these environmental samples. Metabolic assays indicated that the detected genetic capacity was sufficient to account for the overall biodegradative potential observed within the entire sediment microbial community. These data support that, although the reported frequencies were lower than those others have described, the determined frequencies are indicative of the genetic capacity of natural populations in these sediments. While it is likely that additional unidentified degradative pathways and genes exist and are operating in the environment, the data presented in this dissertation support that many of the well understood enzymes and pathways, while primarily studied and defined within laboratory settings, catalyze the majority of the metabolic activity observed in this environmental setting.
Download or read book Cells and Surveys written by National Research Council and published by National Academies Press. This book was released on 2001-01-19 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: What can social science, and demography in particular, reasonably expect to learn from biological information? There is increasing pressure for multipurpose household surveys to collect biological data along with the more familiar interviewer-respondent information. Given that recent technical developments have made it more feasible to collect biological information in non-clinical settings, those who fund, design, and analyze survey data need to think through the rationale and potential consequences. This is a concern that transcends national boundaries. Cells and Surveys addresses issues such as which biologic/genetic data should be collected in order to be most useful to a range of social scientists and whether amassing biological data has unintended side effects. The book also takes a look at the various ethical and legal concerns that such data collection entails.
Download or read book DNA Technology in Forensic Science written by National Research Council and published by National Academies Press. This book was released on 1992-02-01 with total page 199 pages. Available in PDF, EPUB and Kindle. Book excerpt: Matching DNA samples from crime scenes and suspects is rapidly becoming a key source of evidence for use in our justice system. DNA Technology in Forensic Science offers recommendations for resolving crucial questions that are emerging as DNA typing becomes more widespread. The volume addresses key issues: Quality and reliability in DNA typing, including the introduction of new technologies, problems of standardization, and approaches to certification. DNA typing in the courtroom, including issues of population genetics, levels of understanding among judges and juries, and admissibility. Societal issues, such as privacy of DNA data, storage of samples and data, and the rights of defendants to quality testing technology. Combining this original volume with the new update-The Evaluation of Forensic DNA Evidence-provides the complete, up-to-date picture of this highly important and visible topic. This volume offers important guidance to anyone working with this emerging law enforcement tool: policymakers, specialists in criminal law, forensic scientists, geneticists, researchers, faculty, and students.
Download or read book The Metabolic Molecular Bases of Inherited Disease written by Charles R. Scriver and published by New York ; Montreal : McGraw-Hill. This book was released on 2001 with total page 6338 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents clinical, biochemical, and genetic information concerning those metabolic anomalies grouped under inborn errors of metabolism.
Download or read book Biotreatment of Agricultural Wastewater written by Mark E. Huntley and published by CRC Press. This book was released on 2018-01-18 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biotreatment of Agricultural Wastewater is based on a symposium held in Lake Arrowhead, California in 1986, supported by a coalition of federal, state, and local agencies, and sponsored by the engineering firm of Swanson/Oswald Associates (Lafayette, California) and the research and development firms of Aquasearch, Inc. and EcoTechnology Corp. (La Jolla, California). This book is a synopsis of topics covered by world renowned experts on the biology and aquaculture of algae and bacteria and on the engineering of industrial scale systems for biological wastewater treatment and economists that were gathered to evaluate historically proven systems and develop new and innovative approaches to the biological treatment of agriculture wastewater.
Download or read book Environmental Biotechnology for Waste Treatment written by Gary S. Sayler and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of biotechnical processes in control of environmental pollution and in haz ardous waste treatment is viewed as an advantageous alternative or adduct to phys ical chemical treatment technologies. Yet, the development and implementation of both conventional and advanced biotechnologies in predictable and efficacious field applications suffer from numerous technical, regulatory, and societal uncertainties. With the application of modern molecular biology and genetic engineering, there is clear potential for biotechnical developments that will lead to breakthroughs in controlled and optimized hazardous waste treatment for in situ and unit process use. There is, however, great concern that the development of these technologies may be needlessly hindered in their applications and that the fundamental research base may not be able to sustain continued technology development. Some of these issues have been discussed in a fragmented fashion within the research and development community. A basic research agenda has been established to promote a sustainable cross-disciplinary technology base. This agenda includes developing new and improved strains for biodegradation, improving bioanalytical methods to measure strain and biodegradation performance, and providing an in tegrated environmental and reactor systems analysis approach for process control and optimization.
Download or read book Circadian Rhythms written by Michael Young and published by Elsevier. This book was released on 2005-04-04 with total page 951 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences. Circadian Rhythms contains an extensive discussion of genetic and biochemical aspects of circadian rhythms. In this volume organisms such as neurospora, bacteria, drosophila, arabidopsis and mammals are covered. Included are methods in genetics, transcriptional and post-transcriptional regulation, tissue culture, and populations are discussed in detail.* One of the most highly respected publications in the field of biochemistry since 1955 * Frequently consulted, and praised by researchers and reviewers alike * Truly an essential publication for anyone in any field of the life sciences
Download or read book Applications of Molecular Biology in Environmental Chemistry written by Roger A. Minear and published by CRC Press. This book was released on 1995-05-04 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: During this century we have experienced a shift in the leading causes of death from infectious diseases, such as pneumonia and influenza, to chronic diseases, such as heart disease and cancer. Whereas infectious diseases are often related to a single infectious agent, chronic diseases are often related to a combination of environmental (including occupational) chemical exposures and genetic factors. This valuable reference helps the reader to identify these chemical pollutants in environmental matrices such as air, water, food, and soil. It provides improved analytical methods to measure the pollutant, its metabolites, and its various possible adducts in humans. This book presents the latest work designed to assess potential exposure (environmental concentration and activity of pollutants), dose to humans, and a molecular basis for some of the affected biological mechanisms.
Download or read book Molecular Environmental Biology written by Seymour J. Garte and published by CRC Press. This book was released on 1993-11-23 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Environmental Biology is the first book to illustrate molecular biological approaches to major issues in environmental biology. International experts have contributed representative chapters that cover how molecular methods and concepts apply to wildlife management, ecology, pollution control and remediation, and environmental health. Specific topics discussed include the use of molecular techniques in the population biology of wild animals and in the management of fisheries, bioremediation, cloning and characterization of the genes responsible for degradation of PCBs and related environmental pollutants, molecular analysis of aromatic hydrocarbon degradation by soil bacteria, and molecular biological techniques in assessing environmental damage to natural habitats. The book also explores how new molecular approaches can be applied to human disease etiology and epidemiology. Topics discussed in this area include an introduction to molecular epidemiology, the uses of molecular biological markers in cancer risk assessment, specific environmental carcinogens found in foods, measuring DNA adducts and mutation frequencies to assess environmental toxic exposures and effect, and using the extent of gene inducibility as a dosimeter of toxic exposure. This book will interest researchers and students in all fields of environmental biology and environmental medicine. Readers will find information on new techniques and applications of established molecular methodology that will stimulate new research ideas, collaborations, and progress. Researchers will now have a chance to make rapid progress on environmental questions that were previously not even open for exploration.
Download or read book Genetic Analysis written by Philip Mark Meneely and published by Oxford University Press, USA. This book was released on 2014 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt: It uses extended case studies and text boxes to augment the narrative, taking the reader right to the forefront of contemporary research, without losing clarity of explanation and insight.
Download or read book Computational Genomics with R written by Altuna Akalin and published by CRC Press. This book was released on 2020-12-16 with total page 463 pages. Available in PDF, EPUB and Kindle. Book excerpt: Computational Genomics with R provides a starting point for beginners in genomic data analysis and also guides more advanced practitioners to sophisticated data analysis techniques in genomics. The book covers topics from R programming, to machine learning and statistics, to the latest genomic data analysis techniques. The text provides accessible information and explanations, always with the genomics context in the background. This also contains practical and well-documented examples in R so readers can analyze their data by simply reusing the code presented. As the field of computational genomics is interdisciplinary, it requires different starting points for people with different backgrounds. For example, a biologist might skip sections on basic genome biology and start with R programming, whereas a computer scientist might want to start with genome biology. After reading: You will have the basics of R and be able to dive right into specialized uses of R for computational genomics such as using Bioconductor packages. You will be familiar with statistics, supervised and unsupervised learning techniques that are important in data modeling, and exploratory analysis of high-dimensional data. You will understand genomic intervals and operations on them that are used for tasks such as aligned read counting and genomic feature annotation. You will know the basics of processing and quality checking high-throughput sequencing data. You will be able to do sequence analysis, such as calculating GC content for parts of a genome or finding transcription factor binding sites. You will know about visualization techniques used in genomics, such as heatmaps, meta-gene plots, and genomic track visualization. You will be familiar with analysis of different high-throughput sequencing data sets, such as RNA-seq, ChIP-seq, and BS-seq. You will know basic techniques for integrating and interpreting multi-omics datasets. Altuna Akalin is a group leader and head of the Bioinformatics and Omics Data Science Platform at the Berlin Institute of Medical Systems Biology, Max Delbrück Center, Berlin. He has been developing computational methods for analyzing and integrating large-scale genomics data sets since 2002. He has published an extensive body of work in this area. The framework for this book grew out of the yearly computational genomics courses he has been organizing and teaching since 2015.
Download or read book Clocks and Rhythms written by Bruce Stillman and published by CSHL Press. This book was released on 2007 with total page 664 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of a symposium which included sessions and poster sessions covering molecular strategies aimed at understanding the structure and function of biological clocks and rhythms.
Download or read book Plasmid Biology written by Gregory Phillips and published by Washington, DC : ASM Press. This book was released on 2004 with total page 648 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book opens with an essay on the historical perspective of the study of plasmids, reviewing important events and discoveries that have propelled the field forward. The remaining chapters are divided into six sections, detailing basic biological processes such as replication and inheritance functions, specific plasmid systems, plasmid evolution, and use of plasmids as genetic tools. Chapters include use of genomic approaches for the study of plasmid biology, and a review of plasmids from bacteria, archaea, and eukaryotes is presented. In-depth treatment is given to diversity of plasmid systems in the natural environment, and the development of plasmid use in the laboratory is also covered.
Download or read book NMR Spectroscopy in Pharmaceutical Analysis written by Iwona Wawer and published by Elsevier. This book was released on 2008-08-22 with total page 617 pages. Available in PDF, EPUB and Kindle. Book excerpt: For almost a decade, quantitative NMR spectroscopy (qNMR) has been established as valuable tool in drug analysis. In all disciplines, i. e. drug identification, impurity profiling and assay, qNMR can be utilized. Separation techniques such as high performance liquid chromatography, gas chromatography, super fluid chromatography and capillary electrophoresis techniques, govern the purity evaluation of drugs. However, these techniques are not always able to solve the analytical problems often resulting in insufficient methods. Nevertheless such methods find their way into international pharmacopoeias. Thus, the aim of the book is to describe the possibilities of qNMR in pharmaceutical analysis. Beside the introduction to the physical fundamentals and techniques the principles of the application in drug analysis are described: quality evaluation of drugs, polymer characterization, natural products and corresponding reference compounds, metabolism, and solid phase NMR spectroscopy for the characterization drug substances, e.g. the water content, polymorphism, and drug formulations, e.g. tablets, powders. This part is accompanied by more special chapters dealing with representative examples. They give more detailed information by means of concrete examples. - Combines theory, techniques, and concrete applications—all of which closely resemble the laboratory experience - Considers international pharmacopoeias, addressing the concern for licensing - Features the work of academics and researchers, appealing to a broad readership
Download or read book Management of Genetic Syndromes written by Suzanne B. Cassidy and published by John Wiley & Sons. This book was released on 2011-09-20 with total page 1678 pages. Available in PDF, EPUB and Kindle. Book excerpt: The bestselling guide to the medical management of common genetic syndromes —now fully revised and expanded A review in the American Journal of Medical Genetics heralded the first edition of Management of Genetic Syndromes as an "unparalleled collection of knowledge." Since publication of the first edition, improvements in the molecular diagnostic testing of genetic conditions have greatly facilitated the identification of affected individuals. This thorough revision of the critically acclaimed bestseller offers original insights into the medical management of sixty common genetic syndromes seen in children and adults, and incorporates new research findings and the latest advances in diagnosis and treatment of these disorders. Expanded to cover five new syndromes, this comprehensive new edition also features updates of chapters from the previous editions. Each chapter is written by an expert with extensive direct professional experience with that disorder and incorporates thoroughly updated material on new genetic findings, consensus diagnostic criteria, and management strategies. Edited by two of the field's most highly esteemed experts, this landmark volume provides: A precise reference of the physical manifestations of common genetic syndromes, clearly written for professionals and families Extensive updates, particularly in sections on diagnostic criteria and diagnostic testing, pathogenesis, and management A tried-and-tested, user-friendly format, with each chapter including information on incidence, etiology and pathogenesis, diagnostic criteria and testing, and differential diagnosis Up-to-date and well-written summaries of the manifestations followed by comprehensive management guidelines, with specific advice on evaluation and treatment for each system affected, including references to original studies and reviews A list of family support organizations and resources for professionals and families Management of Genetic Syndromes, Third Edition is a premier source to guide family physicians, pediatricians, internists, medical geneticists, and genetic counselors in the clinical evaluation and treatment of syndromes. It is also the reference of choice for ancillary health professionals, educators, and families of affected individuals looking to understand appropriate guidelines for the management of these disorders. From a review of the first edition: "An unparalleled collection of knowledge . . . unique, offering a gold mine of information." —American Journal of Medical Genetics
Download or read book Mededelingen written by and published by . This book was released on 1996 with total page 916 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Evolutionary processes and theory written by Evitar Nevo and published by Elsevier. This book was released on 2012-12-02 with total page 797 pages. Available in PDF, EPUB and Kindle. Book excerpt: Evolutionary Processes and Theory contains the proceedings of a workshop held in Israel in March 1985. Contributors explore evolutionary processes and theory and highlight advances in knowledge concerning differentiation, metabolic and immunological mechanisms, and the molecular biology of the genome. Issues that are being debated are also considered, including the origin and evolution of sexual systems, the genetics of altruism, and general forms and levels of social evolution. This volume is organized into six sections encompassing 33 chapters and begins with an overview of the evolutionary problems of molecular biology. Some chapters are devoted to topics such as the role of gene regulation in evolutionary processes; the structural diversity and evolution of intermediate filament proteins; and adaptation and evolution in the immune system. The next section examines the tempo and mode of molecular evolution, including that of hybrid dysgenesis systems, as well as the statistical aspects of the molecular clock. Later chapters focus on DNA and protein sequences; sexual selection and speciation; and the relation between speciation mechanisms and macroevolutionary patterns. The book also methodically explains population genetics, with particular reference to the altruistic behavior in sibling groups with unrelated intruders, the endosperm evolution in higher plants, and the evolutionary aspects of sexual reproduction in predominantly asexual populations. This book will be of interest to geneticists and molecular biologists.
