Download or read book Detection of DNA Hybridization to Oligonucleotide Arrays on Gold Surfaces Using in Situ Surface Plasmon Resonance and Fluorenscence Imaging Techniques written by Andrew J. Thiel and published by . This book was released on 1997 with total page 638 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Detection of Highly Dangerous Pathogens written by Tanja Kostic and published by John Wiley & Sons. This book was released on 2009-04-29 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by leading experts in the field as part of an interdisciplinary pan-European research program funded by the EU, the results provided in this booklet provide a unique and comprehensive overview of how microarray technology can be used in safely tracking the most highly dangerous pathogens. A must-have for public health agencies focused on bioterrorism as well as all laboratories working with BSL3 and/or BSL 4 agents.
Download or read book Principles of Bacterial Detection Biosensors Recognition Receptors and Microsystems written by Mohammed Zourob and published by Springer Science & Business Media. This book was released on 2008-09-03 with total page 980 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems will cover the up-to-date biosensor technologies used for the detection of bacteria. Written by the world's most renowned and learned scientists each in their own area of expertise, Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems is the first title to cover this expanding research field.
Download or read book Surface Functionalization for Enhanced Fluorescence Detection Surface Plasmon Resonance Imaging and Microscopy written by Jennifer Betsy Fasoli and published by . This book was released on 2015 with total page 67 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work presents several high throughput imaging and analysis techniques performed by fluorescence detection and surface plasmon resonance biosensing. The microarray fabrication methods introduced in this thesis, as well as the DNA functionalization on planar and nanoparticle surfaces, enable and facilitate the real-time study of adsorption events via DNA- DNA hybridization and protein-DNA interaction. Silica deposited on polyolefin film serves as the base for the development of DNA microarray. Fabrication process involves poly(L-glutamic acid) monolayer to react with amine functional groups functionalized on both the silica surface and the probe DNA strands. Covalently surface-bound probes then hybridize with fluorophore-labeled target DNA. Shrinking the microarray with heat significantly enhances the sensitivity of detection via fluorescence signal amplification. Polydopamine, a biomimetic polymer, easily and rapidly grows an adhesive layer to link amine-terminated, single-stranded DNA to gold surface. Self-polymerization of polydopamine simply requires mild reagents and reaction condition. The microarray formed with polydopamine attachment technique reserves its stability and specificity to detect DNA and protein targets by surface plasmon resonance imaging. This one-step microarray fabrication approach, when coupled with signal amplification from DNA-modified gold nanoparticles, empowers the detection limit capable of seeing down to 10 pM target concentration. Electrochemical deposition of polydopamine demonstrates strong control over film localization and thickness. This meticulous polymerization on gold surface at the microscale proves the high toposelectivity of this deposition technique. Amine-modified DNA bound on this polydopamine thin flim hybridizes with fluorophore-tagged DNA to generate fluorescent DNA micropatterns. This electordeposition also forms DNA microarrays used in surface plasmon resonance imaging for DNA-DNA hybridization and DNA-coated gold nanoparticle adsorption measurements. A 814 nm, near-infrared surface plasmon resonance microscope exemplifies a novel, objective-coupled sensing and imaging system at single-nanoparticle scale. This microscope visualizes individual binding occurrence of gold nanoparticles and polystyrene beads. Adsorption analysis presents the calculated surface plasmon resonance signal ([delta]%R) as it varies with particle size. For initial biosensing measurements, the surface plasmon resonance microscopy examines each hybridization-adsorption of DNA-decorated gold nanoparticle on gold surface functionalized with either completely or partially complementary DNA monolayer.
Download or read book Plasmonic Biosensors written by A.B. Dahlin and published by IOS Press. This book was released on 2012-01-11 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book Andreas Dahlin has written a comprehensive and thorough review of plasmonic biosensors that operate by refractometric detection. After an introductory chapter on biosensors, in which he sets out the concepts of biosensing in its application to such areas as proteomics, medical diagnostics and environmental control, he undertakes an integrated coverage of surface chemistry, surface physics and optics, specifically related to the requirements of design of a plasmonic biosensor. Sections on nanoparticle plasmons and surface plasmons follow, leading to a review of SPR technology for biosensing. The optical properties of nanoholes in metal films and other more complicated plasmonic nanostructures are also briefly discussed. Later chapters discuss experimental plasmon spectroscopy and spectral analysis while the final sections discuss topics such as sensor response and the extent to which quantitative measurements can be made. The book introduces a few relatively controversial opinions on some open questions, such as how best to define sensor performance and the practical use of highly miniaturized sensors. Each of the chapters is extensively referenced and contains appropriate illustrations. The book contains a wealth of information that will be highly beneficial to both existing and new users of refractometric sensing techniques in life science research. It will be a valuable resource for post-graduate research students, academic researchers and those working in industry.
Download or read book Tietz Textbook of Clinical Chemistry and Molecular Diagnostics E Book written by Carl A. Burtis and published by Elsevier Health Sciences. This book was released on 2011-12-16 with total page 2259 pages. Available in PDF, EPUB and Kindle. Book excerpt: As the definitive reference for clinical chemistry, Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 5th Edition offers the most current and authoritative guidance on selecting, performing, and evaluating results of new and established laboratory tests. Up-to-date encyclopedic coverage details everything you need to know, including: analytical criteria for the medical usefulness of laboratory procedures; new approaches for establishing reference ranges; variables that affect tests and results; the impact of modern analytical tools on lab management and costs; and applications of statistical methods. In addition to updated content throughout, this two-color edition also features a new chapter on hemostasis and the latest advances in molecular diagnostics. Section on Molecular Diagnostics and Genetics contains nine expanded chapters that focus on emerging issues and techniques, written by experts in field, including Y.M. Dennis Lo, Rossa W.K. Chiu, Carl Wittwer, Noriko Kusukawa, Cindy Vnencak-Jones, Thomas Williams, Victor Weedn, Malek Kamoun, Howard Baum, Angela Caliendo, Aaron Bossler, Gwendolyn McMillin, and Kojo S.J. Elenitoba-Johnson. Highly-respected author team includes three editors who are well known in the clinical chemistry world. Reference values in the appendix give you one location for comparing and evaluating test results. NEW! Two-color design throughout highlights important features, illustrations, and content for a quick reference. NEW! Chapter on hemostasis provides you with all the information you need to accurately conduct this type of clinical testing. NEW! Six associate editors lend even more expertise and insight to the reference. NEW! Reorganized chapters ensure that only the most current information is included.
Download or read book Biomedical Photonics Handbook 3 Volume Set written by Tuan Vo-Dinh and published by CRC Press. This book was released on 2014-07-29 with total page 2524 pages. Available in PDF, EPUB and Kindle. Book excerpt: Shaped by Quantum Theory, Technology, and the Genomics RevolutionThe integration of photonics, electronics, biomaterials, and nanotechnology holds great promise for the future of medicine. This topic has recently experienced an explosive growth due to the noninvasive or minimally invasive nature and the cost-effectiveness of photonic modalities in
Download or read book Biomedical Photonics Handbook written by Tuan Vo-Dinh and published by CRC Press. This book was released on 2014-07-29 with total page 889 pages. Available in PDF, EPUB and Kindle. Book excerpt: Shaped by Quantum Theory, Technology, and the Genomics RevolutionThe integration of photonics, electronics, biomaterials, and nanotechnology holds great promise for the future of medicine. This topic has recently experienced an explosive growth due to the noninvasive or minimally invasive nature and the cost-effectiveness of photonic modalities in
Download or read book Optical Studies of Oligonucleotide Interactions and Gold Nanoparticles Toward Improved DNA Detection at Surfaces written by Yang Gao and published by . This book was released on 2008 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: DNA-based optical biosensors are based on employing tailored surface-bound oligonucleotides probes to detect complementary target strands via hybridization. As interest grows in the application of these biosensors, there is a need for a better understanding of how the surface environment and the oligonucleotide structures affect biosensor performance. The fundamental studies presented in this thesis are a step towards understanding the above effects. By studying the kinetics of oligonucleotide hybridization in solution and on surface, we were able to evaluate of surface effects on DNA duplex formation (hybridization). We found that surface suppresses the rates of duplex formation by 20-40 fold compared to bulk solution, which we attributed to entropic effects. Additionally, we investigated the effect of single-stranded DNA secondary structure on hybridization. For the first time we established a linear trend for a decrease in the association rate constants with an increase in the free energies of the folded single strands for 25-mer oligonucleotides. All of the surface work presented in this study was performed using surface plasmon resonance (SPR) spectroscopy, a label-free technique. The solution measurements were also obtained using unlabeled oligonucleotides by UV spectroscopy with a home-built syringe injection setup. Label-free optical techniques are relatively insensitive. One approach to enhance signals in DNA biosensors is to use gold (Au) nanoparticles (AuNP) as labels at the planar substrates. However, knowledge of the optical properties of the nanoparticles is required to quantify the kinetic and thermodynamic binding of the target molecules. We investigated the dielectric constants for AuNP as a function of wavelength, inter-particle and particle-substrate distance using surface plasmon resonance imaging (SPRI). Preliminary results showed that for 10-run AuNP the dielectric constants agree with the theoretical prediction at the wavelengths studied. We estimate that, by employing AuNP, our SPR detection sensitivity is increased by 300 fold compared to a label free measurement. As expected, the dielectric constants of the nanoparticles are independent of the particle-substrate distance when the distance is greater than the diameter of the nanoparticles. Feasibility to investigate the distance effects at shorter (
Download or read book Biomedical Photonics Handbook Second Edition written by Tuan Vo-Dinh and published by CRC Press. This book was released on 2014-07-29 with total page 894 pages. Available in PDF, EPUB and Kindle. Book excerpt: Shaped by Quantum Theory, Technology, and the Genomics Revolution The integration of photonics, electronics, biomaterials, and nanotechnology holds great promise for the future of medicine. This topic has recently experienced an explosive growth due to the noninvasive or minimally invasive nature and the cost-effectiveness of photonic modalities in medical diagnostics and therapy. The second edition of the Biomedical Photonics Handbook presents fundamental developments as well as important applications of biomedical photonics of interest to scientists, engineers, manufacturers, teachers, students, and clinical providers. The second volume, Biomedical Diagnostics, focuses on biomedical diagnostic technologies and their applications from the bench to the bedside. Represents the Collective Work of over 150 Scientists, Engineers, and Clinicians Designed to display the most recent advances in instrumentation and methods, as well as clinical applications in important areas of biomedical photonics to a broad audience, this three-volume handbook provides an inclusive forum that serves as an authoritative reference source for a broad audience involved in the research, teaching, learning, and practice of medical technologies. What’s New in This Edition: A wide variety of photonic biochemical sensing technologies have already been developed for clinical monitoring of physiological parameters, such as blood pressure, blood chemistry, pH, temperature, and the presence of pathological organisms or biochemical species of clinical importance. Advanced photonic detection technologies integrating the latest knowledge of genomics, proteomics and metabolomics allow sensing of early disease state biomarkers, thus revolutionizing the medicine of the future. Nanobiotechnology has opened new possibilities for detection of biomarkers of disease, imaging single molecules and in situ diagnostics at the single cell level. In addition to these state-of-the art advancements, the second edition contains new topics and chapters including: • Fiber Optic Probe Design • Laser and Optical Radiation Safety • Photothermal Detection • Multidimensional Fluorescence Imaging • Surface Plasmon Resonance Imaging • Molecular Contrast Optical Coherence Tomography • Multiscale Photoacoustics • Polarized Light for Medical Diagnostics • Quantitative Diffuse Reflectance Imaging • Interferometric Light Scattering • Nonlinear Interferometric Vibrational Imaging • Multimodality Theranostics Nanoplatforms • Nanoscintillator-Based Therapy • SERS Molecular Sentinel Nanoprobes • Plasmonic Coupling Interference Nanoprobes Comprised of three books: Volume I: Fundamentals, Devices, and Techniques; Volume II: Biomedical Diagnostics; and Volume III: Therapeutics and Advanced Biophotonics, this second edition contains eight sections, and provides introductory material in each chapter. It also includes an overview of the topic, an extensive collection of spectroscopic data, and lists of references for further reading.
Download or read book Hybrid Nanocomposites written by Kaushik Pal and published by CRC Press. This book was released on 2019-03-11 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding surfaces and interfaces is a key challenge for those working on hybrid nanomaterials and where new imaging and analysis spectroscopy/electron microscopy responses are vital. The variability and site recognition of biopolymers, such as DNA molecules, offer a wide range of opportunities for the self-organization of wire nanostructures into much more complex patterns, while the combination of 1D nanostructures consisting of biopolymers and inorganic compounds opens up a number of scientific and technological opportunities. This book discusses the novel synthesis of nanomaterials and their hybrid composites; nanobiocomposites; transition metal oxide nanocomposites; spectroscopic and electron microscopic studies; social, ethical, and regulatory implications of various aspects of nanotechnology; and significant foreseeable applications of some key hybrid nanomaterials. The book also looks at how technology might be used in the future, estimating, where possible, the likely timescales in which the most far-reaching applications of technology might become a reality. Current research trends and potential future advances, such as nanomaterials, nanometrology, electronics, optoelectronics, and nanobiotechnology, are discussed, in addition to the benefits they are currently providing in the short, medium, and long terms. Furthermore, the book explains the current and possible future industrial applications of nanotechnology, examines some of the barriers to its adoption by industry, and identifies what environmental, health and safety, ethical, or societal implications or uncertainties may arise from the use of the technology, both current and future.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 1998 with total page 652 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Single Molecule Fluorescence Spectroscopy written by Chris Gell and published by OUP Oxford. This book was released on 2006-08-17 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical measurements at the single molecule level under ambient conditions have become almost routine in the past few years. The application of this technology to fundamental studies of heterogeneity in biomolecular structure and dynamics, chemical and biological reaction kinetics, and photophysics provides a rich playground for molecular scientists. The potential use of single molecule detection for nanotechnology and quantum information processing is a new and almost unexplored area. This handbook is intended for those interested in a practical introduction to single molecule investigations using fluorescence techniques and places special emphasis on the practicalities of achieving single molecule resolution, analysing the resulting data and exploration of the applications in biophysics. It is ideal for graduate research students and others embarking on work in this exciting field.
Download or read book Scanning Magnetoresistance Microscopy for Magnetically Labeled DNA Microarrays written by Mei Lin Chan and published by . This book was released on 2010 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemical Abstracts written by and published by . This book was released on 2002 with total page 2540 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Advances in Optical Thin Films written by and published by . This book was released on 2004 with total page 762 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Metal Enhanced Fluorescence written by Chris D. Geddes and published by John Wiley & Sons. This book was released on 2010-06-22 with total page 655 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence.
