Download or read book Detection and Quantitation of Cryptosporidium Parvum in Municipal Water Samples by the Polymerase Chain Reaction written by and published by . This book was released on 1997 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Development and Standardization of a Cryptosporidium Genotyping Tool for Water Samples written by Lihua Xiao and published by American Water Works Association. This book was released on 2006 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt: CD-ROM contains data gathered in the research of this study.
Download or read book Advancing Cryptosporidium Parvum Detection Methodologies written by Mark C. Jenkins and published by American Water Works Association. This book was released on 2002 with total page 90 pages. Available in PDF, EPUB and Kindle. Book excerpt: This study focused on ideal methods of detecting C. parvum oocysts which give low detection limits, specific and sensitive detection in concentraded samples, and differentiation of viable from non-viable oocysts; are usable by a water treatment facility and provide quantitative results within a few hours. Potential useful methods are continuous flow centrifugation, rel-time TaqMan PCR, cell culture inoculation followed by PCR of DNA extration, and FISH.
Download or read book Evaluation of TaqMan Real time PCR for the Detection of Viable Cryptosporidium Parvum Oocysts in Environmental Water Samples written by Melissa A. Cameron and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: ABSTRACT: Cryptosporidium parvum is of growing public health concern due to its ability to survive typical water treatment processes. In order to protect the public from infection, the Environmental Protection Agency developed Method 1623 for the detection of Cryptosporidium oocysts in environmental water samples. Execution of this method is time consuming, and the results do not provide an accurate estimation of viability. Therefore, current research is focused on creating a real-time PCR method for the accurate detection of viable Cryptosporidium parvum in environmental water samples. This thesis presents the development of a real-time PCR method, and the results obtained in its use on field samples. The assay was standardized using multiple dilution series in addition to positive and negative controls. Environmental water samples were tested using this method and Method 1623 for comparison. The results were compared statistically to determine the degree of correlation between methods. The data show that the real-time PCR method correlates well to Method 1623. In addition, the assay was determined to be more cost effective and less labor intensive than Method 1623. Although these early findings are promising, additional research and development are needed before the proposed assay can be used in industry.
Download or read book Evaluation of Genotyping Techniques for Crytosporidium Parvum written by Paul A. Rochelle and published by American Water Works Association. This book was released on 2001 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt: A research teams reports on its efforts to develop and implement DNA fingerprinting techniques to characterize and differentiate the parvus species of protozoal parasite that is ubiquitous in rivers and lakes, is relatively resistant to chlorine disinfection at commonly used concentrations, and can prove fatal for immune compromised people who become infected. They evaluate fingerprinting methods based on polymerase chain reaction, determine which method is most suitable for determining strains or isolates within a species, implement the most appropriate molecular method for determining whether the isolates can be differentiated based on its animal or human host or geographical source, and determine the particular source of the protozoa contamination in source water. The report is not indexed. c. Book News Inc.
Download or read book Detection of Cryptosporidium Parvum Oocysts in Soil Using the Polymerase Chain Reaction written by Juliet E. Bryant and published by . This book was released on 1995 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Detection and Quantification of Cryptosporidium Oocysts in Environmental Samples written by Megha Duggal and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Public Health Microbiology written by John F. T. Spencer and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 531 pages. Available in PDF, EPUB and Kindle. Book excerpt: Public Health Microbiology: Methods and Protocols is focused on microorganisms that can present a hazard to human health in the course of everyday life. There are chapters dealing with organisms that are directly pathogenic to humans, including bacteria, viruses, and fungi; on organisms that produce toxins during growth in their natural habitats; on the use of bacteriocins produced by such organisms as lactobacilli and bifidobacteria; as well as several chapters on hazard analysis, the use of disinfectants, microbiological analysis of cosmetics, and microbiological tests for sanitation equipment in food factories. Additional chapters look at the use of animals (mice) in the study of the various characteristics of milk and their relationships with lactic acid bacteria in particular. Other chapters focus on special methods for determining particular components of milk. In particular, in Parts I and II, on bacterial and viral pathogens, special attention is given to methods for PCR detection of genes with resistance to tetracycline, as well as to Salmonella enterica; for identification and typing of Campylobacter coli; for detection of the abundance of enteric viruses, hepatitis A virus, and rotaviruses in sewage, and of bacteriophages infecting the O157:H7 strain of Escherichia coli. Part III offers methods for computerized analysis and typing of fungal isolates, for isolation and enumeration of fungi in foods, and for the determination of aflatoxin and zearalenone.
Download or read book Indicators for Waterborne Pathogens written by National Research Council and published by National Academies Press. This book was released on 2004-06-19 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent and forecasted advances in microbiology, molecular biology, and analytical chemistry have made it timely to reassess the current paradigm of relying predominantly or exclusively on traditional bacterial indicators for all types of waterborne pathogens. Nonetheless, indicator approaches will still be required for the foreseeable future because it is not practical or feasible to monitor for the complete spectrum of microorganisms that may occur in water, and many known pathogens are difficult to detect directly and reliably in water samples. This comprehensive report recommends the development and use of a "tool box" approach by the U.S Environmental Protection Agency and others for assessing microbial water quality in which available indicator organisms (and/or pathogens in some cases) and detection method(s) are matched to the requirements of a particular application. The report further recommends the use of a phased, three-level monitoring framework to support the selection of indicators and indicator approaches.Â
Download or read book Handbook of Nucleic Acid Purification written by Dongyou Liu and published by CRC Press. This book was released on 2009-01-14 with total page 572 pages. Available in PDF, EPUB and Kindle. Book excerpt: An Indispensable Roadmap for Nucleic Acid Preparation Although Friedrich Miescher described the first isolation of nucleic acid in 1869, it was not until 1953 that James Watson and Francis Crick successfully deciphered the structural basis of DNA duplex. Needless to say, in the years since, enormous advances have been made in the study of nucleic a
Download or read book Microbial Waterborne Pathogens written by Thomas E. Cloete and published by IWA Publishing. This book was released on 2004-08-31 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the developed world, the connection between water, hygiene and health is taken for granted. However, for the less fortunate majority, access to potable water is non-existent and remains a daily struggle. Bacteria, viruses and parasites in contaminated water cause water borne disease. Of concern are the so-called new emerging pathogens, contributing to water borne disease, one of the biggest human tragedies, killing more than 5 million people each year. About 2.3 billion people suffer from diseases linked to contaminated water and some 6,000 people die daily as a result of this. Some 60% of all infant mortality worldwide is linked to water-related infectious and parasitic diseases. Treating water before use can eliminate most of these waterborne pathogens. The essential starting point is knowledge of the disease-causing organisms, the detection techniques, and the epidemiology, which is the focus of this book. Microbial Waterborne Pathogens provides up-to-date coverage of waterborne microbial pathogens including traditional and emerging pathogens and the latest molecular detection techniques. The link between climate and disease is covered in the book and indicates future approaches to dealing with this important area as we face the effects of global climate change All the existing and emerging pathogens including bacteria, viruses and protozoa are reviewed. The characteristics of each organism are discussed in detail as well as their epidemiology. Methods for the detection of these pathogens, traditional and new are presented. Microbial Waterborne Pathogens provides students, academics and practitioners with a complete reference book on the microbiological quality and safety of potable water.
Download or read book Toxoplasma Gondii written by Louis M. Weiss and published by Academic Press. This book was released on 2013-08-10 with total page 1109 pages. Available in PDF, EPUB and Kindle. Book excerpt: This 2e of Toxoplasma gondii reflects the significant advances in the field in the last 5 years, including new information on the genomics, epigenomics and proteomics of T. gondii as well as a new understanding of the population biology and genetic diversity of this organism. T. gondii remains the best model system for studying the entire Apicomplexa group of protozoans, which includes Malaria, making this new edition essential for a broad group of researchers and scientists. Toxoplasmosis is caused by a one-celled protozoan parasite known as T. gondii. The infection produces a wide range of clinical syndromes in humans, land and sea mammals, and various bird species. Most humans contract toxoplasmosis by eating contaminated, raw or undercooked meat (particularly pork), vegetables, or milk products; by coming into contact with the T. gondii eggs from cat feces; or by drinking contaminated water. The parasite damages the ocular and central nervous systems, causing behavioral and personality alterations as well as fatal necrotizing encephalitis. It is especially dangerous for the fetus of an infected pregnant woman and for individuals with compromised immune systems, such as HIV-infected patients. Completely updated, the 2e presents recent advances driven by new information on the genetics and genomics of the pathogen Provides the latest information concerning the epidemiology, diagnosis, treatment and prevention of toxoplasmosis Offers a single-source reference for a wide range of scientists and physicians working with this pathogen, including parasitologists, cell and molecular biologists, veterinarians, neuroscientists, physicians, and food scientists
Download or read book Cryptosporidium and Cryptosporidiosis written by Ronald Fayer and published by CRC Press. This book was released on 2007-12-25 with total page 579 pages. Available in PDF, EPUB and Kindle. Book excerpt: From the microscopic observation of infection to the widespread application of molecular techniques in taxonomy and epidemiology, to the genome sequencing of two major species and advances in biochemistry, phylogeny, and water treatment, new information on this fascinating genus continues to mount as we discover and utilize the latest scientific te
Download or read book Cryptosporidium From Molecules to Disease written by R.C.A. Thompson and published by Elsevier. This book was released on 2003-12-17 with total page 469 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the relatively short period since Cryptosporidium was recognised as a human pathogen, and that it could be transmitted in water as well as directly between animals and people, it has been the subject of intense investigations. Its status as an opportunistic pathogen, especially in AIDS patients, and the lack of effective anti-cryptosporidial drugs have served to emphasise the public health importance of this organism. This has to some extent overshadowed the fact that Cryptosporidium is also an important pathogen of domestic animals and wildlife. In recent years, the application of molecular biology and culture techniques have had an enormous impact on our understanding of the aetiological agents of cryptosporidial infections and our ability to study the causative agents in the laboratory. As a consequence, a wealth of information and novel data has been produced during the last 3-4 years, particularly in the areas of taxonomy, biology, pathogenesis, epidemiology - particularly zoonotic and water borne transmission, and treatment. It is thus very timely to bring together in this book the international research community involved to review the major advances in research and identify the important research priorities for the future, thus enabling as wide an audience as possible to benefit from and share in this comprehensive look at Cryptosporidium and cryptosporidiosis.
Download or read book Giardia and Cryptosporidium written by Guadalupe Ortega-Pierres and published by CABI. This book was released on 2009 with total page 518 pages. Available in PDF, EPUB and Kindle. Book excerpt: Giardia and Cryptosporidium are both parasites of considerable global interest due to the gastrointestinal problems these organisms can cause in humans as well as domestic and wild animals. Despite its long history as the causative agent of giardiasis, the clinical and zoonotic significance of Giardia infections in humans and animals is only beginning to emerge with the application of molecular tools. Less is known about Cryptosporidium and studies seek to understand the impact of infection in livestock and other animals, its epidemiology and zoonotic significance. Presenting a comprehensive overview of recent research this book draws on the experience of experts in all fields of Giardia and Cryptosporidium research.
Download or read book Molecular Detection of Human Parasitic Pathogens written by Dongyou Liu and published by CRC Press. This book was released on 2012-07-05 with total page 899 pages. Available in PDF, EPUB and Kindle. Book excerpt: Traditionally, laboratory identification of parasites has relied upon various phenotypic procedures that detect their morphological, biological, and immunological features. Because these procedures tend to be time-consuming and technically demanding, molecular methods based on nucleic acid amplification technologies have been increasingly utilized for rapid, sensitive, and specific characterization of parasites. The large number of original and modified molecular protocols that have been developed over the years creates a dilemma for those attempting to adopt the most appropriate protocol for streamlined identification and detection of human pathogenic organisms of interest. Part of a four-volume collection, Molecular Detection of Human Parasitic Pathogens provides a reliable and comprehensive resource on the molecular detection and identification of major human parasitic pathogens. This volume contains expert contributions from international scientists involved in human parasitic pathogen research and diagnosis. Following a similar format throughout, each chapter includes: A brief review on the classification, biology, epidemiology, clinical features, and diagnosis of an important pathogenic parasitic genus/group An outline of clinical sample collection and preparation procedures and a selection of representative stepwise molecular protocols A discussion on further research needs relating to improved diagnoses of major human parasitic pathogens This versatile reference on molecular detection and identification of major human parasitic pathogens is an indispensable tool for upcoming and experienced medical, veterinary, and industrial laboratory scientists engaged in parasite characterization. It is also suitable as a textbook for undergraduate and graduate students majoring in parasitology.
Download or read book Guidelines for Drinking water Quality written by World Health Organization and published by World Health Organization. This book was released on 1993 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume describes the methods used in the surveillance of drinking water quality in the light of the special problems of small-community supplies, particularly in developing countries, and outlines the strategies necessary to ensure that surveillance is effective.
