Download or read book Design Synthesis and Characterization of Fluorescent Hyperbranched Polyglycerols and Organic Nanoparticles with Covalently Bound Anti fading Agents written by and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fluorescent Organic Nanoparticles written by Waseem A. Wani and published by Springer. This book was released on 2018-09-27 with total page 72 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a critical review of recent advances in the development of fluorescent organic nanoparticles as materials of choice for the design and fabrication of sensors, bioimaging agents and drug delivery systems. The properties and functions of nanoparticles differ significantly from those of their parent entities or their bulk phases. Two of their most important features are their increased surface-to volume ratio, and the formation of surface structures differing from those in their bulk phases. In addition, the book discusses the synthesis of fluorescent conjugated polymers, self-assembled fluorescent nanoparticles, polydopamine nanoparticles, and aggregation-induced-emission or aggregation-induced-emission enhancement nanomaterials. In closing, the book provides an outlook on future research and development in fluorescent organic nanoparticles as smart materials with an impressive range of potential applications.
Download or read book Fluorescent Organic Nanoparticles written by Waseem A. Wani and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a critical review of recent advances in the development of fluorescent organic nanoparticles as materials of choice for the design and fabrication of sensors, bioimaging agents and drug delivery systems. The properties and functions of nanoparticles differ significantly from those of their parent entities or their bulk phases. Two of their most important features are their increased surface-to volume ratio, and the formation of surface structures differing from those in their bulk phases. In addition, the book discusses the synthesis of fluorescent conjugated polymers, self-assembled fluorescent nanoparticles, polydopamine nanoparticles, and aggregation-induced-emission or aggregation-induced-emission enhancement nanomaterials. In closing, the book provides an outlook on future research and development in fluorescent organic nanoparticles as smart materials with an impressive range of potential applications.
Download or read book Fluorescent Analogs of Biomolecular Building Blocks written by Marcus Wilhelmsson and published by John Wiley & Sons. This book was released on 2016-03-30 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent Analogs of Biomolecular Building Blocks focuses on the design of fluorescent probes for the four major families of macromolecular building blocks. Compiling the expertise of multiple authors, this book moves from introductory chapters to an exploration of the design, synthesis, and implementation of new fluorescent analogues of biomolecular building blocks, including examples of small-molecule fluorophores and sensors that are part of biomolecular assemblies.
Download or read book Design Synthesis and Characterization of Conjugated Polymers as Fluorescent Sensors written by Yan Zhang and published by . This book was released on 2003 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Design and Synthesis of Ultra bright Organic Nanoparticles ONPs for Bioimaging written by Paolo Pagano and published by . This book was released on 2017 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nowadays the use of bright luminescent nano-objects in biological environment is a topic that is gaining more and more importance, especially for biomedical applications such as imaging, the rapyand diagnostic. So far, numerous studies have been conducted with gold nanoparticles, silica nanoparticles (doped or functionalized with organic molecules), as well as semiconductor nanoparticles (quantum dots, i.e., QDs). However, most of these nanoparticles suffer from drawbacks (in terms of stability, biocompatibility, eco-toxicity or degradability). On the other hand, several nontoxic fluorescent molecular probes have been widely used, but most of the time their brightness remain modest in biological environments compared to QDs. Our idea is to engineer new organicchromophores with tunable emission wavelength (from visible to near infrared) for further preparation of organic fluorescent nanoparticles (so called FONs) that display giant one-photon and two-photonbrightness, as well as good colloidal and chemical stability, and suitable photostability for in vitro andin vivo imaging. As such, these FONs would represent interesting alternatives to QDs for use in bioimaging. This manuscript describes the synthesis and characterization of new classes of fluorescent molecules specifically engineered as building blocks for the fast preparation of such nanoparticles byself-aggregation in water. The FONs were fully characterized from both morphological and photophysical points of view and further used in bioimaging.
Download or read book Design Synthesis and Characterization of Mechanosensitive Dual Fluorescent Dyes as Potential Probes for Biological Applications written by Susantha Ganegamage and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chapter 3 of this dissertation explores a hypothesis that leverages the seesaw photophysical model for N-aryl (2,3- and 1,8-) naphthalimides. The Heagy group initially developed this model to understand the dual fluorescence effect concerning the electron donor or electron-withdrawing substituents. This study envisions the design and synthesis of novel dual fluorescence molecules for a new class of N-aryl-phenanthridinone dyes. The applied photophysical model further investigates these dyes with a substitution pattern opposite naphthalimide systems. Interestingly, predictive computational modeling shows that the substituent pattern, used previously for 2,3-naphthalimides, relies on these groups getting placed on rings in opposite positions for N-aryl-phenanthridinones. Chapter 4, provides an overall conclusion of the and future directions for both parts of the research. In chapter 5, a complete description of experimental data is provided for the characterization of all the dye systems presented. This data includes a detailed synthesis procedure, characterization, purity analysis by HPLC, absorptivity calculation, fluorescence lifetime measurements, quantum yield determination, biological evaluation and fluorescence titration.
Download or read book Synthesis Characterization and Optical Properties of Colloidal Organic Dye containing Fluorescent Core shell Silica Nanoparticles written by Erik Herz and published by . This book was released on 2009 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since fluorescence was first observed in a solution containing quinine sulfate in 1845, methods employing fluorescent materials have abounded. Today, fluorescent probes are widely used in many fields, including biological imaging and security. For all applications brightness, stability, size control and compatibility are key parameters for probe design. Recent research to address these parameters has been focused on synthesizing nanoparticle materials. A class of those materials, core-shell silica nanoparticles, covalently encapsulating organic dyes within the core, have been found to be particularly effective in meeting the need for brighter, more stable probes, while also providing size and compatibility control. This dissertation will describe how I have built on previous research by focusing on the synthesis, characterization and optical properties of core-shell silica nanoparticles that span the full spectral range, from ultraviolet to near-infrared emission. Through scattering corrected comparisons of coumarin dye-containing nanoparticles and dye in aqueous solution, relative quantum yield enhancements of an order of magnitude were measured. Similar brightness enhancements were observed when large Stokes-shift dyes were incorporated into the particles. By creating a graded series of Stokes-shifted particles, single-excitation functionality was added to the fluorescent core-shell silica nanoparticle platform, to allow for spectral multiplexing. Since both light scattering and autofluorescence backgrounds in biological samples tend to be stronger at shorter wavelengths, new probe design has swiftly moved toward near-infrared (NIR) excitation and emission. To this end, NIR fluorescent silica nanoparticles with emissions up to 800nm were synthesized and dye structure-optical property correlations were investigated. Using a self-built fluorescence correlation spectroscopy (FCS) instrument, cyanine dye containing particles of the NIR series were investigated in further depth. The FCS allows for measurement of the size, brightness and concentration of the particles in solution and, with small modifications to the beam-path also provides information on the fluorescence stability of the particles in solution, which is of particular importance in NIR probes. Photostability in aqueous solution was investigated for the NIR dye Cy5 and compared to the dye incorporated into particles, leading to findings that will impact future probe designs.
Download or read book Design Synthesis and Characterization of New Two photon Absorbing 2PA Fluorescent Dyes and Bioconjugates and Their Applications in Bioimaging written by Carolina D. Andrade and published by . This book was released on 2010 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of new multiphoton absorbing materials has attracted the attention of researchers for the last two decades. The advantages that multiphoton absorbing materials offer, versus their one-photon absorbing counterparts, rely on the nature of the nonlinearity of the absorption process, where two photons are absorbed simultaneously offering increased 3D resolution, deeper penetration, and less photobleaching and photodamage as a result of a more confined excitation. The applications of efficient two-photon absorbing materials have been extensively expanding into the fields of photodynamic therapy, microscopy, and optical data storage. One of the fields where an increased interest in multiphoton absorbing materials has been most evident is in bioimaging, in particular, when different cellular processes and organelles need to be studied by fluorescence microscopy. The goal of this research was to develop efficient two-photon absorption (2PA) compounds to be used in fluorescence bioimaging, meaning that such compounds need to posses good optical properties, such as high fluorescence quantum yield, 2PA cross section, and photostability. In the first chapter of this dissertation, we describe the synthesis and structural characterization of a new series of fluorescent donor-acceptor and acceptor-acceptor molecules based on the fluorenyl ring system that incorporated functionalities such as alkynes and thiophene rings, through efficient Pd-catalyzed Sonogashira and Stille coupling reactions, in order to increase the length of the conjugation in our systems. These new molecules proved to have high two-photon absorption (2PA), and the effect of these functionalities on their 2PA cross section values was evaluated. Finally, their use in two-photon fluorescence microscopy (2PFM) imaging was demonstrated. One of the limitations of the compounds described in Chapter 1 was their poor water solubility; this issue was addressed in Chapter 2. The use of micelles in drug delivery has been shown to be an area of increasing interest over the last decade. In the bioimaging field, it is key to have dye molecules with a high degree of water solubility to enable cells to uptake the dye. By enclosing a hydrophobic dye in Pluronic® F-127 micelles, we developed a system that facilitates the use of 2PA molecules (typically hydrophobic) in biological systems for nonlinear biophotonic applications, specifically to image the lysosomes. Furthermore, we report in this chapter the efficient microwave-assisted synthesis of the dye used in this study. In addition, linear photophysical and photochemical parameters, two-photon absorption (2PA), and superfluorescence properties of the dye studied in Chapter 2, were investigated in Chapter 3. The steady-state absorption, fluorescence, and excitation anisotropy spectra of this dye were measured in several organic solvents and aqueous media. In Chapter 4, we describe the preparation and the use of an efficient and novel two-photon absorbing fluorescent probe conjugated to an antibody that confers selectivity towards the vascular endothelial growth factor receptor 2 (VEGFR-2) in porcine aortic endothelial cells that express this receptor (PAE-KDR). It is known that this receptor is overexpressed in certain cancer processes. Thus, targeting of this receptor will be useful to image the tumor vasculature. It was observed that when the dye was incubated with cells that do not express the receptor, no effective binding between the bioconjugate and the cells took place, resulting in very poor, nonspecific fluorescence images by both one and two-photon excitation. On the other hand, when the dye was incubated with cells that expressed VEGFR-2, efficient imaging of the cells was obtained, even at very low concentrations (0.4[micrometer]). Moreover, incubation of the bioconjugate with tissue facilitated successful imaging of vasculature in mouse embryonic tissue.
Download or read book Design Synthesis and Characterization of Novel Bifunctional Ligands for Incorporation Into a Fluorescent Polymer Sensor written by Jordan Claire Turner Reddel and published by . This book was released on 2011 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Synthesis and Characterization of Fluorescent Protein Decorated Nanoparticles written by Vidmantas Lapiene and published by . This book was released on 2010 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Design Synthesis and Characterization of Novel Hydrophilic Fluorene based Derivatives for Bioimaging Applications written by Dao Nguyen and published by . This book was released on 2009 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this work, hydrophilic fluorene-based derivatives that contain ethylene oxide substituents, have been synthesized and characterized for potential use as new fluorophores for bioimaging applications and for fluorescence sensing of heavy metals. Symmetrical and unsymmetrical fluorene derivatives based on structural types of acceptor-[pi]-acceptor, acceptor-[pi]-donor, and donor-[pi]-donor were characterized by TGA, UV-vis absorption, fluorescence emission, lifetime, anisotropy, and two-photon absorption (2PA) cross section. They were found to possess high thermal stability, high photostability, high fluorescence quantum yields, and generally large two-photon absorption cross sections, making them quite suitable for new probes in single-photon absorption and two-photon absorption fluorescence microscopy imaging. Novel hydrophilic fluorene derivatives were synthesized from fluorene in multiple steps employing the metal-catalyzed Heck coupling reaction, the Stille reaction, the Sonogashira reaction, the Ullmann condensation reaction, and "click" chemistry. To increase the hydrophilicity of the new compounds, ethylene oxide substituents were utilized for to impart water solubility. An alternative alkylation methodology using ethyleneoxy tosylates was introduced for the synthesis of ethylene oxide-containing fluorene derivatives. Several of these hydrophilic derivatives were incubated into various cell lines as new probes for both conventional and two-photon absorption fluorescence bioimaging. These compounds were biocompatible, exhibiting low cytotoxicity as determined by cell viability studies, and displayed colocalization for selected cellular organelles. In addition, hydrophilic bis(1,2,3-triazolyl)fluorene derivatives were found to exhibit sensitive fluorescence responses in the presence of certain heavy metal, and were selective for sensing zinc and mercury over other a number of other metal ions relevant to living cells or other biological environments. The UV-vis absorption and fluorescence emission spectra of the complexes exhibited a blue-shifted absorption and emission for selective metal chelation upon binding to zinc and mercury(II) ions, resulting in an approximately two-fold enhanced fluorescence response. Fluorescence titration studies revealed that the complexes of 1:2 and 1:3 ligand to metal formed with binding constant values of 108 and 1014 for zinc and mercury ions, respectively. Finally, preliminary experiments were performed to explore the possibility of employing select hydrophilic fluorene-based derivatives in the synthesis of hydrophilic fluorescent gold nanoparticles. Although results are very preliminary, the aim is to use such materials for other biomedical applications, such as surface enhanced scattering resonance and noninvasive photothermal therapy to diagnose and to treat cancers. Thus, this research had led to the discovery of alternative methodologies for synthesis of hydrophilic fluorene derivatives by alkylation with alkyl tosylates and synthesis of hydrophilic fluorescent molecule capped gold nanoparticles. Furthermore, several novel hydrophilic fluorene-based derivatives were synthesized and characterized for their linear and nonlinear photophysical properties, and are now available for further examination of their bioimaging and sensing applications.
Download or read book Molecular Design Synthesis and Characterization of Polymeric Nonlinear Optical Materials written by Yi Jin and published by . This book was released on 1992 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Design Synthesis and Characterization of Bio electroactive Hybrids written by Onur Y. Kas and published by ProQuest. This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Design Synthesis and Characterization of Second Order Nonlinear Optical Polymers written by Zhiyong Liang and published by . This book was released on 1995 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Design Synthesis and Characterization of Conjugated Polymers for Photovoltaics and Electrochromics written by Kim Bini and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
