Download or read book Design and Synthesis of Fluorescence Turn on Probes for Iron written by Randy K. Jackson and published by . This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Design and Synthesis of Fluorescent Probes written by Prabin Rai and published by . This book was released on 2013 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fundamental objective of this project is to design, synthesize, and characterize fluorescent dyes, which may be utilized in super resolution imaging techniques. In Chapters 1, 2 and 3, we concentrated on photoswitchable rhodamine dyes. We synthesized several rhodamine dyes and increased their water solubility, installed a bioconjugation unit and, more importantly, we optimized the absorption properties (close to 400 nm) of the rhodamine spirolactams in their closed state and studied their basic photophysical properties as well. In Chapter 4, we synthesized azido-DCDHF fluorogens that can be converted to the bright state after a 1,3-dipolar cycloaddition reaction between an azide-Ph-DCDHF and a strained alkene. We synthesized some strained alkenes, which may speed up the kinetics in 1,3-dipolar cycloaddition. This chemical method of turning the dyes from dark to bright state is a new dimension in the bioconjugation arena. In Chapter 5, we synthesized Nile red derivatives which can switch to a bright state from a dark state by collision on the cell surface utilizing PAINT methodology. We expected that the design of new Nile red derivatives may have better properties than the parent Nile red. Besides the PAINT technique, we worked on some active control of emission by enzymatic cleavage of fluorescent dyes in a dark state to the bright state, which can be utilized in super resolution imaging. Related to the 1,3-dipolar cycloaddition reaction between azido-DCDHF and norbornene, we have examined recently popularized tetrazine chemistry. We linked pyridyl tetrazines to DCDHF with short spacer. In Chapter 6, we describe the preparation of co-crystals between perfluorophenazine and several polynuclear aromatic compounds/polynuclear heteroaromatic compounds. In Chapter 7 we describe the preparation of some partially fluorinated heteropolynuclear aromatic compounds such phenzaine and acridine class of compounds for possible use in organic semiconductors.

Download or read book Principles of Bioinorganic Chemistry written by Stephen J. Lippard and published by University Science Books. This book was released on 1994 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of unnatural metals - which have been introduced into human biology as diagnostic probes and drugs - is another active area of tremendous medical significance.

Download or read book Design and Synthesis of ESIPT Based Fluorescent Probes for the Sensing of Potential Analytes With CD Copy written by Komal and published by . This book was released on 2021 with total page 44 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Design of Iron Specific Fluorescent Probes written by Yongmin Ma and published by . This book was released on 2005 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Fluorescent Probes in the Detection of Insecticides in Water written by Chester M. Himel and published by . This book was released on 1973 with total page 56 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Design and Synthesis of Fluorescent Probes for Detection of Cyanide and Cysteine and Their Applications in Living Cells written by and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Design and Synthesis of Fluorescent Probe for Thiol and Its Applications in Living Cells written by and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Design and Synthesis of Cyanine based Near infrared Fluorescent Probes and Their Application in Bioimaging written by 譚毅 and published by . This book was released on 2018 with total page 177 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Fluorescent Energy Transfer Nucleic Acid Probes written by Vladimir V. Didenko and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. These probes signal detection of their targets by changing either the intensity or the color of their fluorescence. Not surpr- ingly, these luminous, multicolored probes carry more flashy names than their counterparts in the other fields of molecular biology. In recent years, fluor- cent probes and assays, which make use of energy transfer, have multiplied at a high rate and have found numerous applications. However, in spite of this explosive growth in the field, there are no manuals summarizing different p- tocols and fluorescent probe designs. In view of this, the main objective of Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is to provide such a collection. Oligonucleotides with one or several chromophore tags can form fluor- cent probes capable of energy transfer. Energy transport within the probe can occur via the resonance energy transfer mechanism, also called Förster tra- fer, or by non-Förster transfer mechanisms. Although the probes using Förster transfer were developed and used first, the later non-Förster-based probes, such as molecular beacons, now represent an attractive and widely used option. The term “fluorescent energy transfer probes” in the title of this book covers both Förster-based fluorescence resonance energy transfer (FRET) probes and probes using non-FRET mechanisms. Energy transfer probes serve as molecule-size sensors, changing their fluorescence upon detection of various DNA reactions.

Download or read book Design Synthesis and Sensing Properties of Chiral Amine based Fluorescent Probes written by Xiaobo Zhou and published by . This book was released on 2012 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Design and Evaluation of Novel Iron Specific Fluorescent Probes with Biological Applications written by Wei Luo and published by . This book was released on 2006 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Design and Synthesis of a Novel Fluorescent Redox Potential Probe Based on De Novo Peptide Design written by Kyung H. Lee and published by . This book was released on 2004 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Design and Synthesis of Photo induced Electron Transfer Based Fluorescent Probes for Pyruvate and Zinc Detection written by Charles D. Cook and published by . This book was released on 2007 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: The design and synthesis of fluorescent chemosensor probes based on photoinduced electron transfer is discussed. First, the design of a series of probes to detect the presence of divalent metal cations is discussed. Next, the results for an existing probe for divalent cations are examined. Computational chemistry is used to provide a possible answer to its selectivity. This method of examination suggests that the probes selectivity is due to the bonding involved in analyte coordination. Then, the design of a series of probes to detect the presence of mono-, di-, and, trivalent hydrocarbon anions is discussed. From this discussion, the synthesis of a single probe from that series as well as characterization and the spectrophotometric results from its titration with pyruvate, malate, and citrate is discussed, respectively. The probe was found to be more selective and sensitive toward pyruvate. Work is currently underway to design and synthesize other series of probes to detect this family of anion.

Download or read book Synthesis and Characterization of Novel Fluorescent Sensors Based on Terpyridine Benzoxazole and Benzothiazole Chromophores written by Xiaoman Bi and published by . This book was released on 2017 with total page 173 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past few decades, there has been significant interest in developing fluorescent probes, because they are useful tools for biological studies. As effective analytical techniques, fluorescent probes utilize distinct advantages offered by fluorescence detection in terms of sensitivity, selectivity, and fast response time. When fluorescent probes interact selectively with target molecules, ions or biological specimens, they can generate large optical responses. Since most ions or molecules, such as Zn2+, Ca2+, or pyrophosphate ion (PPi), are non-fluorescent, chemosensors having analyte binding-triggered fluorescence are appealing in many fields, like analytical chemistry, clinical biochemistry, medicine, and environmental science.This dissertation is devoted to the design, synthesis, and characterization of novel fluorescent sensors for Zn2+ and its associated applications. Chapter II of this dissertation presents several novel terpyridine-based fluorescent sensors with different substituents affecting the electronic and steric nature of the terpyridine (tpy) fluorophore. Sensors are designed to establish the correlation between sensor structure and its photophysical properties. Low temperature fluorescence is used to evaluate the essential role of intramolecular charge transfer (ICT) in zinc binding-induced fluorescence changes. The tpy molecular fragment has a relatively large [pi]-conjugated system which enables the potential [pi-pi] interaction between two tpy platforms and affects the fluorescence of tpy ligands. Chapter III introduces a dimeric tpy ligand containing two tpy fragments connected via a meta-phenylene unit. The detailed spectroscopic study shows that this ligand displays an attractive fluorescence turn-on, in sharp contrast to mono(tpy) ligand that shows fluorescence quenching upon binding Zn2+. The result suggests the existence of delicate structural influences on fluorescence of tpy derivatives.Chapter IV is devoted to 2-(2'-hydroxyphenyl)-1,3-benzoxazole (HBO) and 2-(2'-hydroxyphenyl)-1,3-benzothiazole (HBT) derivatives featured with a structural potential of excited-state intramolecular proton transfer (ESIPT). The study reveals additional information on the binding of HBO or HBT to metal cations, which aids the sensor design for Zn2+ and PPi detection. The molecular design aims to realize ESIPT process control upon complexation with an analyte. Chapter V is devoted to the synthesis of bis(HBO) derivatives which bind Zn2+ selectively and emit near-infrared (NIR) fluorescence as a consequence of metal ion binding-induced ESIPT turn-on. Preliminary cell stain experiment was conducted and indicated the potential biological applications.

Download or read book Synthesis of Fluorescent Molecules and Their Applications as Viscosity Sensors Metal Ion Indicators and Near infrared Probes written by Mengyuan Wang and published by . This book was released on 2014 with total page 119 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Chapter 2, a new BAPTA (O, O'-bis־בםימןנטומשל)ופטשלומוחלשדןל־,ﬞ ,ﬞ ׳ﬞ, ׳ﬞ־פופעב בדופיד בדיה) במה �ֶ�ֳִ� (4,4־היזלץןען־4־גןעב־3ב,4ב־היבתב־ף־ימהבדומו)־גבףוה דבלדיץם ימהידבפןע, �ֶָ�ֶ־3, יף עונןעפוה. ָמוק ףשמפטופיד עןץפו קבף וםנלןשוה פן ףיםנליזש גןפט ףשמפטוףיף במה נץעיזידבפיןמ, קטידט פומה פן גו לןק שיולהימח במה דץםגועףןםו זןע �ֶָ� ָהועיצבפיצוף. �נןמ ורדיפבפיןמ, ב 1.5־זןלה ימדעובףו ימ זלץןעוףדומדו ימפומףיפש ימ גץזזוע דןמפבימימח 39 [םץ]�œ בֵ2+ במה ב 3־זןלה ימדעובףו ימ זלץןעוףדומדו ימפומףיפש ימ גץזזוע דןמפבימימח 1 ׁבֵ2+ קבף ןגףועצוה; םןהוףפ גץפ נעןםיףימח זלץןעוףדומדו פץעמ־ןמ ומטבמדוםומפף. מֳ טֵבנפוע 3, ב מוקלש־הוףיחמוה ץמףשםםופעידבל ףסץבעבימו השו, ��3, קבף ףשמפטוףיתוה. ָןמו־נןפ ףשמפטוףיף קבף וםנלןשוה עוףץלפימח ימ ב 10% שיולה, ב עוףץלפ פטבפ יף חומועבללש סץיפו זבצןעבגלו זןע פטו דעובפיןמ ןז ץמףשםםופעידבל ףסץבעבימוף �טןפןנטשףידבל במה נטןפןדטוםידבל דטבעבדפועיתבפיןמ קבף דןמהץדפוה ימ צבעיןץף ףןלצומפף, במה ב 678 מם בגףןענפיןמ םבריםץם במה ב 692 מם וםיףףיןמ םבריםץם קועו עודןעהוה ימ �ִׁ� ףןלץפיןמ קיפט ב זלץןעוףדומדו סץבמפץם שיולה ןז 0.32. מֳ ציפען דולל ףפץהיוף הוםןמףפעבפוה פטבפ ��3 דבמ גו ץףוה בף ב מובע־�ֳ נעןגו זןע גיןיםבחימח.

Download or read book Metals in Cells written by Valeria Culotta and published by John Wiley & Sons. This book was released on 2016-03-16 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last three decades a lot of research on the role of metals in biochemistry and medicine has been done. As a result many structures of biomolecules with metals have been characterized and medicinal chemistry studied the effects of metal containing drugs. This new book (from the EIBC Book Series) covers recent advances made by top researchers in the field of metals in cells [the “metallome”] and include: regulated metal ion uptake and trafficking, sensing of metals within cells and across tissues, and identification of the vast cellular factors designed to orchestrate assembly of metal cofactor sites while minimizing toxic side reactions of metals. In addition, it features aspects of metals in disease, including the role of metals in neuro-degeneration, liver disease, and inflammation, as a way to highlight the detrimental effects of mishandling of metal trafficking and response to "foreign" metals. With the breadth of our recently acquired understanding of metals in cells, a book that features key aspects of cellular handling of inorganic elements is both timely and important. At this point in our understanding, it is worthwhile to step back and take an expansive view of how far our understanding has come, while also highlighting how much we still do not know. The content from this book will publish online, as part of EIBC in December 2013, find out more about the Encyclopedia of Inorganic and Bioinorganic Chemistry, the essential online resource for researchers and students working in all areas of inorganic and bioinorganic chemistry.