Download or read book Design and Synthesis of Cyanine based Near infrared Fluorescent Probes and Their Application in Bioimaging written by 譚毅 and published by . This book was released on 2018 with total page 177 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book DESIGN AND DEVELOPMENT OF NEAR INFRARED FLUORESCENT PROBES FOR SENSING PH HYPOXIA AND PEROXYNITRITE written by and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract : pH and hypoxia levels are two important diagnostic biomarkers in biological systems and abnormal levels will cause many diseases. The metabolism and transportation of peroxynitrite in biological systems involve a series of important enzymes and proteins. Peroxynitrite generation represents a crucial pathogenic mechanism in conditions such as stroke, myocardial infarction, chronic heart failure, diabetes, circulatory shock, chronic inflammatory diseases, cancer, and neurodegenerative disorders. As a result, it is very important to detect and evaluate the levels of pH, hypoxia, and peroxynitrite in vivo to study the functionality of enzymes and provide practical applications for cancer diagnosis and therapy. Fluorescence detection and imaging offer a sensitive non-invasive method because it provides clear images of cellular three-dimensional structures, and can illustrate cellular multiple overlapping structures simultaneously, and enable image-guided surgery. However, it is challenging to design fluorescent probes with good fluorescence signals, low background noise, and multifunctional detection capability. In order to overcome these drawbacks, we have designed and synthesized a series of excellent fluorescent probes to detect pH, hypoxia, and peroxynitrite with good fluorescence signals, fast responses, low background noise, and multifunctional detection capabilities. The probes combine advantages of near-infrared imaging such as low fluorescence background interference, less photodamage to cells and tissues, and deep-tissue penetration with ratiometric imaging, this latter aspect reduces systematic errors with solely intensity-based fluorescent probes caused by fluctuations in the excitation light source, variations in probe concentrations, and different probe compartmental localizations. We have developed a new backbone for making near-infrared fluorescent probes. This new backbone has a reactive chlorine group for modifying the detectors. Based on this new backbone, we introduced a pH-responsive detector to prepare three pH-sensitive near-infrared fluorescent probes with high fluorescence quantum yields. We designed and synthesized a series of fluorescent probes on detecting hypoxia level. The nitro group was introduced directly in the conjugation system of the fluorophore, which make it fast response and ultra-sensitive. Additionally, by changing the electron-withdrawing abilities of the substitution group on 9-position of acridine moiety, the fluorescent probes show a significant different detection limit and sensitivity towards nitroreducatse. For third project, we developed a fluorescent probe responding to peroxynitrite. The probe contains a near-infrared fluorophore and a coumarin moiety and connected through a conjugated triple bond linker. The ester group of the coumarin moiety in the conjugation system can enhance the sensitivity of the ONOO- detection. For the fourth project, we developed multi-response fluorescent probe. This probe can detect pH and ONOO- with different fluorescent changes. Base pH condition can induce an on-off fluorescent change on the probe, and the ONOO- can generate a ratiometric fluorescent change.

Download or read book Aggregation Induced Emission AIE written by Jianwei Xu and published by Elsevier. This book was released on 2022-04-17 with total page 698 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aggregation-Induced Emission (AIE): A Practical Guide introduces readers to the topic, guiding them through fundamental concepts and the latest advances in applications. The book covers concepts, principles and working mechanisms of AIE in AIE-active luminogens, with different classes of AIE luminogens reviewed, including polymers, three-dimensional frameworks (MOFs and COFs) and supramolecular gels. Special focus is given to the structure-property relationship, structural design strategies, targeted properties and application performance. The book provides readers with a deep understanding, not only on the fundamental principles of AIE, but more importantly, on how AIE luminogens and AIE properties can be incorporated in material development. Provides the fundamental principles, design and synthesis strategies of aggregation induced emission materials Reviews the most relevant applications in materials design for stimuli-responsive materials, biomedical applications, chemo-sensing and optoelectronics Emphasizes structural design and its connection to aggregation induced emission properties, also exploring the structure-property relationship

Download or read book Near Infrared Dyes for High Technology Applications written by S. Daehne and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes the results of the NATO Advanced Research Workshop on "Syntheses, Optical Properties, and Applications of Near-Infrared (NIR) Dyes in High Technology Fields" held at Trest (Czech Republic) from September 24 to 27, 1997. It was attended by 52 scientists from 16 countries, among them 18 participants from 8 Eastern-Europe. The Workshop has been stimulated by the great progress that has been made in utilizing NIR dyes in high technology areas. This has been triggered by the availability of reliable. vel)' small, and inexpensive laser diodes emitting in the NIR region which raise the analytical detection limits by several orders of magnitude, allow time-resolved measurements for temporal discrimination between spectrally similar species, and make possible miniaturization as well as remote sensing through space and by fiber optical techniques. Furthermore, NIR dyes proved to be vel)' important in other high technology fields such as laser physics, optical recording techniques, nonlinear optics, biochemistty and medicine.

Download or read book Near Infrared Dyes for High Technology Applications written by S. Daehne and published by Springer Science & Business Media. This book was released on 1998-06-30 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes the results of the NATO Advanced Research Workshop on "Syntheses, Optical Properties, and Applications of Near-Infrared (NIR) Dyes in High Technology Fields" held at Trest (Czech Republic) from September 24 to 27, 1997. It was attended by 52 scientists from 16 countries, among them 18 participants from 8 Eastern-Europe. The Workshop has been stimulated by the great progress that has been made in utilizing NIR dyes in high technology areas. This has been triggered by the availability of reliable. vel)' small, and inexpensive laser diodes emitting in the NIR region which raise the analytical detection limits by several orders of magnitude, allow time-resolved measurements for temporal discrimination between spectrally similar species, and make possible miniaturization as well as remote sensing through space and by fiber optical techniques. Furthermore, NIR dyes proved to be vel)' important in other high technology fields such as laser physics, optical recording techniques, nonlinear optics, biochemistty and medicine.

Download or read book Spectroscopy and Kinetics written by James S. Mattson and published by Marcel Dekker. This book was released on 1973 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Synthesis and Application of Novel Near Infrared Cyanine Dyes and Optical Imaging Agents written by Neil Norouzi and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Near Infrared Applications in Biotechnology written by Ramesh Raghavachari and published by CRC Press. This book was released on 2020-06-16 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores developments in techniques in diagnostics, DNA sequencing, bioanalysis of immunoassays, and single-molecule detection. It promotes the measurement, identification, monitoring, analysis, and application of near-infrared spectroscopy (NIR) to medical and pharmaceutical advances. The text also considers noninvasive methods of NIR for successful, cost-effective, and prompt diagnoses of diseases.

Download or read book Study on the Novel Near infrared Fluorescent Probes Based on Indole Heptamethine Cyanine Dyes written by 孙春龙 and published by . This book was released on 2019 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Introduction to Fluorescence Sensing written by Alexander P. Demchenko and published by Springer Nature. This book was released on 2020-12-01 with total page 673 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides systematic knowledge of basic principles in the design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Fluorescence is the most popular technique in chemical and biological sensing because of its ultimate sensitivity, high temporal and spatial resolution and versatility that enables imaging within the living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response up to detection of single molecules. Its application areas range from control of industrial processes to environment monitoring and clinical diagnostics. Being a guide for students and young researchers, it also addresses professionals involved in active basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.

Download or read book Design and Synthesis of Donor acceptor donor Xanthene based Near Infrared I and Shortwave Infrared SWIR Dyes for Biological Imaging written by Chathuranga Rathnamalala and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Small molecule organic dyes with absorption and emission in the near infrared region (NIR) attracted much attention for various applications such as dye sensitized solar cells, fluorescent guided surgery, stimuli responsive bioimaging and photodynamic therapy. Dyes with high absorption and emission in the NIR region are beneficial for stimuli responsive bioimaging due to the deeper penetration of NIR light, less cell damage, high resolution, and low background autofluorescence from biomolecules. Of the many small molecule dyes, xanthene-based dyes exhibit outstanding photophysical properties and good stimuli response for use in bioimaging applications. However, absorption and emission of the xanthene dyes lie in the visible region, which limit their applications in cellular imaging. Many of the NIR dyes have very poor fluorescence; consequently, an alternative approach to fluorescent imaging is photoacoustic imaging that uses sound waves to generate pictures of deep tissues. In this dissertation, we discuss the utility of xanthene based NIR dyes as photoacoustic imaging contrast agents for multiplex imaging and deep tissue nitric oxide sensing in the drug-induced liver injury. Chapter I discuss the fundamentals of fluorescence and photoacoustic imaging, background of the xanthene dyes and other fluorescent dyes, and the design strategies to develop NIR xanthene-based dyes. Chapter II is based on our approach to the design and synthesis of NIR xanthene-based dyes by C-H bond functionalization, with the first example being Rhodindolizine, which absorb and emits in NIR II or short-wave infrared (SWIR) region. In chapter III, we describe the design and synthesis of thienylpiperidine xanthene-based NIR and shortwave-infrared (SWIR) dyes for the photoacoustic imaging. One dye in particular (XanthCR-880) boasts a strong PA signal at 880 nm with good biological compatibility and photostability, yields multiplexed imaging with an aza-BODIPY reference dye, and is detected at a depth of 4 cm. In chapter IV, we report a series of SWIR dyes based on a dibenzazepine donor conjugated to thiophene (SCR-1, SCR-4), thienothiophene (SCR-2, SCR-5), and bithiophene (SCR-3, SCR-6). We leverage the fact that SCR-1 undergoes a bathochromic shift when aggregated to develop a ratiometric nanoparticle for nitric oxide (NO) (rNP-NO). rNP-NO was used to successfully perform in vivo studies to visualize pathological levels of nitric oxide in a drug-induced liver injury model via deep tissue SWIR photoacoustic (PA) imaging. Chapter V describes another series of xanthene-based dyes with a thiophene þ̀± spacer and several different donors. UV-Vis absorption studies were performed after converting the dyes to the opened form with trifluoracetic acid. These novel XanthCR-TD dyes exhibit absorption maxima in NIR I region from 700 - 900 nm.

Download or read book Functional Fluorescent Materials written by Vivek Mishra and published by CRC Press. This book was released on 2024-06-17 with total page 347 pages. Available in PDF, EPUB and Kindle. Book excerpt: Functional Fluorescent Materials: Applications in Sensing, Bioimaging, and Optoelectronics explains functional molecular probes (organic/inorganic materials, polymers, nanomaterials), with a focus on those that represent spectroscopic properties with detection of different analytes and specific roles in molecular recognition and their applications. It broadly covers molecular recognition to applications of fluorescence reporters, starting from optoelectronic properties of materials, detection of heavy metals, through biological macromolecules, and further to a living cell, tissue imaging, and theranostics. Features: • Covers different aspects of fluorescence spectroscopy ranging from chemical, physical, and biological aspects along with optoelectronic properties, mechanisms, and applications. • Describes all types of chemical and functionalized fluorescent nanomaterials. • Provides additional information on different kinds of fluorescence reporters. • Explains the concept of fluorescence spectroscopy and its role in human health care. • Discusses changes in static and dynamic properties of fluorescent probes and molecular recognitions. This book is aimed at graduate students and researchers in materials, chemical engineering, and engineering physics.

Download or read book Fluorescent Probes written by and published by Academic Press. This book was released on 2021-05-13 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent Probes, Volume 48 in the Methods in Microbiology series, highlights new advances in the field, with this new volume presenting interesting chapters on important topics, including Hydrogel microarray technology as a tool for clinical diagnostics, The use of probes and bacteriophages for bacteria detection, Probes used with point-of-care microfluidic devices for pathogen detection, Methods for combining FIB/SEM with three-dimensional fluorescence microscopy using CLEM approaches, Probes and Microbes, Microbial signatures associated with cancers, Fluorescent Aptamers for Detection and Treatment of Pathogenic Bacteria and Cancer, Labelled and Unlabeled Probes for Pathogen Detection with Molecular Biology Methods and Biosensors, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Microbiology series

Download or read book Synthesis of Fluorescent Molecules and Their Applications as Viscosity Sensors Metal Ion Indicators and Near infrared Probes written by Mengyuan Wang and published by . This book was released on 2014 with total page 119 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Chapter 2, a new BAPTA (O, O'-bis־בםימןנטומשל)ופטשלומוחלשדןל־,ﬞ ,ﬞ ׳ﬞ, ׳ﬞ־פופעב בדופיד בדיה) במה �ֶ�ֳִ� (4,4־היזלץןען־4־גןעב־3ב,4ב־היבתב־ף־ימהבדומו)־גבףוה דבלדיץם ימהידבפןע, �ֶָ�ֶ־3, יף עונןעפוה. ָמוק ףשמפטופיד עןץפו קבף וםנלןשוה פן ףיםנליזש גןפט ףשמפטוףיף במה נץעיזידבפיןמ, קטידט פומה פן גו לןק שיולהימח במה דץםגועףןםו זןע �ֶָ� ָהועיצבפיצוף. �נןמ ורדיפבפיןמ, ב 1.5־זןלה ימדעובףו ימ זלץןעוףדומדו ימפומףיפש ימ גץזזוע דןמפבימימח 39 [םץ]�œ בֵ2+ במה ב 3־זןלה ימדעובףו ימ זלץןעוףדומדו ימפומףיפש ימ גץזזוע דןמפבימימח 1 ׁבֵ2+ קבף ןגףועצוה; םןהוףפ גץפ נעןםיףימח זלץןעוףדומדו פץעמ־ןמ ומטבמדוםומפף. מֳ טֵבנפוע 3, ב מוקלש־הוףיחמוה ץמףשםםופעידבל ףסץבעבימו השו, ��3, קבף ףשמפטוףיתוה. ָןמו־נןפ ףשמפטוףיף קבף וםנלןשוה עוףץלפימח ימ ב 10% שיולה, ב עוףץלפ פטבפ יף חומועבללש סץיפו זבצןעבגלו זןע פטו דעובפיןמ ןז ץמףשםםופעידבל ףסץבעבימוף �טןפןנטשףידבל במה נטןפןדטוםידבל דטבעבדפועיתבפיןמ קבף דןמהץדפוה ימ צבעיןץף ףןלצומפף, במה ב 678 מם בגףןענפיןמ םבריםץם במה ב 692 מם וםיףףיןמ םבריםץם קועו עודןעהוה ימ �ִׁ� ףןלץפיןמ קיפט ב זלץןעוףדומדו סץבמפץם שיולה ןז 0.32. מֳ ציפען דולל ףפץהיוף הוםןמףפעבפוה פטבפ ��3 דבמ גו ץףוה בף ב מובע־�ֳ נעןגו זןע גיןיםבחימח.

Download or read book Systematic Exploration of Indolizine Based Small Fluorescent Molecules written by Youngjun Lee and published by Springer. This book was released on 2018-08-16 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis describes an in-depth study of an indolizine-based fluorophore, from understanding of its structure-photophysical property relationship to its application as a useful biological reporter. Organic fluorophores have been extensively used in the field of molecular biology owing to their excellent photophysical property, suitable cell permeability, and synthetic flexibility. Understanding of the structure-photophysical property relationship of a given fluorophore often paves the road to the development of valuable molecular probes to visualize and transcribe biological networks. In this thesis, respective chapters deal with molecular design, organic synthesis, structure-property analysis, and quantum-mechanical interpretation of unexplored family of indolizine-based molecules. This systematic exploration has led to rational development of a new microalgae lipid droplet probe, colorful bioorthogonal fluorogenic probes, and a bright mitochondrial probe, working under live cell conditions. Harnessing the optical properties of a given fluorophore has been an important topic for a couple of decades, both in industry and in academia. This thesis provides useful insights for the improvement and development of unique small fluorescent materials, or optical materials.

Download or read book Advances in Bionanomaterials II written by Stefano Piotto and published by Springer Nature. This book was released on 2020-05-29 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents multidisciplinary research focusing on the analysis, synthesis, and design of bio and nanomaterials. Merging biophysics, biochemistry, and bioengineering perspectives, it discusses the basic properties of materials and their interaction with biological systems; the development of new medical devices, such as implantable systems; and new algorithms and methods for modeling the mechanical, physical, and biological properties of biomaterials. The book gathers the proceedings of the 3rd International Conference on Bio and Nanomaterials, held on September 29–October 3, 2019, on an MSC cruise ship navigating the Mediterranean Sea. It particularly highlights Horizon 2020 projects, covering topics such as novel synthetic strategies for nanomaterials, the implementation of bio- and smart materials for pharmacological and medical purposes, as well as environmental applications. Intended for a broad audience of academics and professionals, it offers a comprehensive and timely snapshot of the field of biomaterials. In addition to a set of innovative theories together with the necessary practical tools for their implementation, it also addresses the current challenges in the field, fostering new discussions and possible future collaborations between diverse groups.

Download or read book Electrogenerated Chemiluminescence written by Allen J. Bard and published by CRC Press. This book was released on 2004-07-20 with total page 707 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first source on this expanding analytical science, this reference explores advances in the instrumentation, design, and application of techniques with electrogenerated chemiluminescence (ECL), examining the use and impact of ECL-based assays in clinical diagnostics, life science research, environmental testing, food and water evaluation, and th