Download or read book Design and Site Directed Mutagenesis of Streptavidin Mutants for Study of Two dimensional Protein Crystallization written by Andrew Joseph Leitz and published by . This book was released on 2000 with total page 64 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Effects of Interaction Modifications in Two dimensional Streptavidin Crystallization written by Szu-Wen Wang and published by . This book was released on 1999 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Influence of PH on Two dimensional Crystals of Streptavidin written by Michael Todd Yatcilla and published by . This book was released on 1998 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Site directed Mutagenesis and Protein Engineering written by M. Rafaat El-Gewely and published by Elsevier Science & Technology. This book was released on 1991 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book primarily tackles the issues concerning the factors controlling the formation of the active form of proteins. Protein engineering is a multi-disciplinary field emerging as a result of interaction between fields of research such as: X-ray crystallography, biochemistry, molecular biology, and genetics. An overview of these interactions and their connection to protein engineering is presented in this volume, pointing the way to future research where protein engineers of the future will be able to design proteins of any needed function.
Download or read book Two dimensional Crystallization of Streptavidin on Solid Substrates written by Chengfei Lou and published by . This book was released on 2006 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Streptavidin as a Host for Cu II Complexes written by Jonathan D. Paretsky and published by . This book was released on 2015 with total page 98 pages. Available in PDF, EPUB and Kindle. Book excerpt: In nature, metal ions are utilized in many proteins and enzymes in order to perform a variety of chemical transformations that are essential to maintaining human life. The range of selectivity and functionality can be attributed to the unique environment surrounding each active site, which allow for control over not only the primary coordination sphere—containing atoms covalently bound to the metal ion, but also the secondary coordination sphere (microenvironment)—made up of non-covalent interactions such as hydrogen bonds (H-bonds). Although nature is very efficient at these chemical processes, achieving the same level of selectivity is difficult in synthetic systems. In order to reproduce the functions found in metalloproteins it is necessary to maintain control over both the primary and secondary coordination spheres of synthetic systems as seen within active sites. To accomplish this, typically a rigid organic framework is constructed that will coordinate to a metal ion and additionally provide a supporting network of non-covalent interactions: intramolecular H-bonds. The Borovik group has performed extensive research the area of secondary coordination sphere effects around transition metal complexes and has developed a multitude of ligand frameworks that bind metal ions and incorporate intramolecular H-bond donors or acceptors that can stabilize exogenous ligands bound to the transition metal (TM) complexes. While these systems have been extremely effective and have been used to prepare unique high-valent metal-oxo/hydroxo species, they fall short in being able to provide extensive networks of H-bonds and long-range interactions found in microenvironments of metalloproteins. To address these challenges and gain further control over the secondary coordination sphere, this dissertation presents the development of new research program within the Borovik group in collaboration with the Ward lab: the development of artificial metalloproteins using biotin-Streptavidin (Sav) technology. Although Sav does not naturally contain metal ions, TM complexes of interest can be attached to biotin and inserted into the protein. This is an attractive feature, because of Sav’s exceptionally high affinity for biotin , which can be exploited for the use of installing biotinylated metal complexes. Furthermore, the selective binding of biotin provides a reproducible anchor for TM complexes within the active site of protein. In addition to being able to chemically modify a ligand, the protein is amenable to site directed mutagenesis, which allows for alterations of the microenvironment around the TM complexes. This combined chemogenetic approach allows for enhanced control over primary and secondary coordination spheres of TM complexes. The first portion of this document describes the study of biotinylated copper(II) complexes with varied linker lengths between the Cu(II) complex and biotin. Utilizing different linker lengths was proposed to allow for: 1) to design discrete monomeric species confined within separate subunits or 2) to create a bimetallic systems at the dimer interface. Additionally, different mutants of Sav varying the amino acid residue at a site proximal to the Cu(II) complex were investigated to observe the protein effects on the secondary coordination sphere of the TM complex. Changing the serine at the112 position to either lysine, aspartate, or cysteine had drastic effects on the microenvironment of the Cu(II) complex. This was studied through Uv-Vis and electronic paramagnetic resonance (EPR) spectroscopies, and X-ray diffraction studies (XRD). XRD studies explicitly showed the molecular structures of the Cu(II) complexes bound within Sav and the effects of linker length changes and site directed mutagenesis. This research has shown proof of concept that Cu(II) complexes can be prepared within Sav and different microenvironments can be reproducibly attained with different linker lengths and protein mutants, and my work has established this methodology as a promising new research program within the group. Another way of harnessing the dimer-of-dimers nature of Sav would be to prepare a TM complex linked to two biotin molecules to potentially bridge across two subunits of the protein. As reproducibility and rigidity of a binding environment is essential to selectivity in chemical transformations, this was envisioned as a method of firmly anchoring a TM complex within the protein to be studied further. To probe this hypothesis a bis-biotinylated Schiff-base was prepared and its binding to Sav studied using HABA titrations and gel electrophoresis. It was shown that instead of bridging two subunits in a homotetramer, the ligand and its complexes bridged separate Sav molecules to form oligomers of proteins. Prior to developing the biotin-Sav program, I investigated the use of well-established tripodal, tetradentate ligands within the Borovik lab. Previous research within the lab had shown that the Fe(II) complexes of these ligands were capable of activating aryl azides to form Fe(III)-amido compounds via a putative Fe(IV)-imido intermediates. It was proposed that this would be amenable to a catalytic system wherein a C–H bond within an aryl azide substrate could be activated by an Fe(IV)-imido and I anticipated subsequent radical rebound would form a new C–N bond. However, the system did not demonstrate the ability to perform C–H amination.
Download or read book Proceedings of the National Academy of Sciences of the United States of America written by and published by . This book was released on 1995-11 with total page 1254 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Avidin biotin Technology written by Meir Wilchek and published by . This book was released on 1990 with total page 794 pages. Available in PDF, EPUB and Kindle. Book excerpt: The avidin-biotin complex has been used for isolation (affinity chromatography), localization (affinity cytochemistry, cell cytometry, and blotting technology), and diagnostics (immunoassay, histopathology, and gene probes). Recently, usage of the system has been extended to include other areas. This volume covers these new applications and methodologies including hybridoma technology, bioaffinity sensors, affinity targeting, and drug delivery, as well as cross-linking, immobilization, and fusogenic studies.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1997 with total page 1850 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bio Applications of Nanoparticles written by Warren C.W. Chan and published by Springer Science & Business Media. This book was released on 2009-09-29 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edited book highlights the central players in the Bionanotechnology field - which are the nanostructures and biomolecules. It provides broad examples of current developments in Bionanotechnology research and is an excellent introduction to the field. The book describes how nanostructures are synthesized and details the wide variety of nanostructures available for biological research and applications. Examples of the unique properties of nanostructures are provided along with the current applications of these nanostructures in biology and medicine. The final chapters of the book describe the toxicity of nanostructures.
Download or read book Advanced Chemical Biology written by Howard C. Hang and published by John Wiley & Sons. This book was released on 2023-04-03 with total page 806 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Chemical Biology The modern approach to teaching chemical biology Advanced Chemical Biology is organized around the central dogma of life, progressing from genes to proteins and higher-order cellular structures, including core application areas such as imaging, chemical genetics, activity-based protein profiling, and natural product discovery and biosynthesis. Advanced topics and applications in, e. g., microbiology, developmental biology, and neurobiology, are covered in separate sections. Every chapter is homogeneous in style and layout, consisting of a short historical introduction followed by a description of the underlying concepts and a selection of recent examples of how the concept has been turned into practice. The subdivision of the contents into core and supplemental chapters enables a flexible use in teaching, both for a one-semester and a two-semester course. Written by authors and editors coming from the leading scientific institutions that have developed the concepts and technologies for this discipline, Advanced Chemical Biology includes specific information on topics like: DNA function, synthesis and engineering, chemical approaches to genome integrity, and RNA function, synthesis, and probing Chemical approaches to transcription and RNA regulation in vivo, chemical biology of genome engineering, and peptide/protein synthesis and engineering Directed evolution for chemical biology, chemical biology of cellular metabolism, chemical biology of lipids, and protein post-translational modifications Chemical glycobiology, chemical and enzymatic modification of proteins, genetic code expansion, bio-orthogonal chemistry, and cellular imaging With its broad scope and focus on turning concepts into applications, Advanced Chemical Biology is an excellent starting point for anyone entering the field and looking for a guide to the wide range of available methods and strategies that chemical biology has to offer. With a Foreword by Nobel Laureate Carolyn Bertozzi.
Download or read book Advanced Topics on Crystal Growth written by Sukarno Ferreira and published by BoD – Books on Demand. This book was released on 2013-02-20 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: Crystal growth is the key step of a great number of very important applications. The development of new devices and products, from the traditional microelectronic industry to pharmaceutical industry and many others, depends on crystallization processes. The objective of this book is not to cover all areas of crystal growth but just present, as specified in the title, important selected topics, as applied to organic and inorganic systems. All authors have been selected for being key researchers in their field of specialization, working in important universities and research labs around the world. The first section is mainly devoted to biological systems and covers topics like proteins, bone and ice crystallization. The second section brings some applications to inorganic systems and describes more general growth techniques like chemical vapor crystallization and electrodeposition. This book is mostly recommended for students working in the field of crystal growth and for scientists and engineers in the fields of crystalline materials, crystal engineering and the industrial applications of crystallization processes.
Download or read book Communicated Abstracts written by and published by . This book was released on 1996 with total page 710 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2006 with total page 860 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Annual Scientific Report written by Howard Hughes Medical Institute and published by . This book was released on 1988 with total page 748 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Current Advances in Protein Biochemistry written by and published by . This book was released on 1996 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Directed Enzyme Evolution Advances and Applications written by Miguel Alcalde and published by Springer. This book was released on 2017-02-14 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on some of the most significant advances in enzyme engineering that have been achieved through directed evolution and hybrid approaches. On the 25th anniversary of the discovery of directed evolution, this volume is a tribute to the pioneers of this thrilling research field, and at the same time provides a comprehensive overview of current research and the state of the art. Directed molecular evolution has become the most reliable and robust method to tailor enzymes, metabolic pathways or even whole microorganisms with improved traits. By mirroring the Darwinian algorithm of natural selection on a laboratory scale, new biomolecules of invaluable biotechnological interest can now be engineered in a manner that surpasses the boundaries of nature. The volume is divided into two sections, the first of which provides an update on recent successful cases of enzyme ensembles from different areas of the biotechnological spectrum, including tryptophan synthases, unspecific peroxygenases, phytases, therapeutic enzymes, stereoselective enzymes and CO2-fixing enzymes. This section also provides information on the directed evolution of whole cells. The second section of the book summarizes a variety of the most applicable methods for library creation, together with the future trends aimed at bringing together directed evolution and in silico/computational enzyme design and ancestral resurrection.
