Download or read book Cryopreservation of Plant Germplasm I written by Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 526 pages. Available in PDF, EPUB and Kindle. Book excerpt: The germ plasm of numerous plant species, especially those of forest trees, some agricultural crops, and medicinal plants, is endangered and threatened with extinction. This depletion of germplasm pools and the shrinkage of naturally occurring genetic resources have caused international concern. Conventionally, the germplasm of plants is conserved through seeds, tubers, roots, corms, rhizomes, bulbs, cuttings, etc. However, the germ plasm of a number of trees and plantation crops (such as coconut, cocao, coffee, oil palm, rubber, mango, horse chestnut, etc. ) cannot be preserved since their seed are short-lived (recalcitrant). Likewise, germplasm of vegetatively propagated crops (such as potato and cassava) cannot be stored on a long term basis and has to be grown and multiplied periodically in nurseries and fields. The plants are thus exposed to unpredictable weather conditions and diseases, with the result that instances are known where entire genetic stocks are lost. Therefore, unconventional methods are being developed for the storage and international exchange of germplasm. For this purpose in vitro cultures have been employed, but they can only enable short-to medium term preservation; moreover, cell cultures upon repeated subculture undergo genetic erosion. In view of the recent developments in the in vitro induction of genetic variability through somaclonal variation, somatic hybridization, recombinant DNA technology, etc. , new methods need to be employed for the storage of desirable cultures. In this regard freeze preservation of cells in liquid nitrogen (-196 0q, like that of semen, enables long-term storage, theoretically, for an indefinite period of time.
Download or read book Cryopreservation of Plant Germplasm written by and published by . This book was released on 1995 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cryopreservation of Plant Germplasm II written by L.E. Towill and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ex situ preservation of germplasm for higher plant species has been accom plished using either seeds or clones, but storage of these under typical condi tions does not provide the extreme longevities that are needed to minimize risk of loss. Costs of maintenance and regeneration of stocks are also high. Systems that provide virtually indefinite storage should supplement existing methods and it is within this context that cryopreservation is presented. The use of low temperature preservation was initially more a concern of medicine and animal breeding, and was expanded to plants in the 1970s. Sur vival after cryogenic exposure has now been demonstrated for diverse plant groups including algae, bryophytes, fungi and higher plants. If survival is com monplace, then the eventual application is a cryopreservation system, whereby cells, tissues and organs are held indefinitely for use, often in the unforeseen future. The increasing interest and capabilities for application could not have occurred at a more opportune time since expanding human populations have placed unprecedented pressures on plant diversity. This book emphasizes cry opreservation of higher plants and was initially driven by the concern for loss of diversity in crops and the recognized need that this diversity would be essential for continued improvement of the many plants used by society for food, health and shelter. The interest in cryopreservation has been expanded by conservationists and their concerns for retaining, as much as possible, the diversity of natural populations. The need for cryopreservation, thus, is well established.
Download or read book Plant Cryopreservation A Practical Guide written by Barbara B.M. Reed and published by Springer Science & Business Media. This book was released on 2007-10-31 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cryopreservation has proven to be an important tool for the storage and conservation of plant genetic resources. This book is a unique resource for plant scientists, providing more than 100 ready-to-use cryopreservation protocols for plant types from algae and bryophytes to a range of flowering plants. It includes techniques for diverse plant parts such as dormant buds, pollen, and apical meristems and for cell types such as suspension and callus cultures.
Download or read book Cryopreservation of Plant Germplasm I written by Y. P. S. Bajaj and published by Springer. This book was released on 1995-09-29 with total page 514 pages. Available in PDF, EPUB and Kindle. Book excerpt: The germ plasm of numerous plant species, especially those of forest trees, some agricultural crops, and medicinal plants, is endangered and threatened with extinction. This depletion of germplasm pools and the shrinkage of naturally occurring genetic resources have caused international concern. Conventionally, the germplasm of plants is conserved through seeds, tubers, roots, corms, rhizomes, bulbs, cuttings, etc. However, the germ plasm of a number of trees and plantation crops (such as coconut, cocao, coffee, oil palm, rubber, mango, horse chestnut, etc. ) cannot be preserved since their seed are short-lived (recalcitrant). Likewise, germplasm of vegetatively propagated crops (such as potato and cassava) cannot be stored on a long term basis and has to be grown and multiplied periodically in nurseries and fields. The plants are thus exposed to unpredictable weather conditions and diseases, with the result that instances are known where entire genetic stocks are lost. Therefore, unconventional methods are being developed for the storage and international exchange of germplasm. For this purpose in vitro cultures have been employed, but they can only enable short-to medium term preservation; moreover, cell cultures upon repeated subculture undergo genetic erosion. In view of the recent developments in the in vitro induction of genetic variability through somaclonal variation, somatic hybridization, recombinant DNA technology, etc. , new methods need to be employed for the storage of desirable cultures. In this regard freeze preservation of cells in liquid nitrogen (-196 0q, like that of semen, enables long-term storage, theoretically, for an indefinite period of time.
Download or read book Plant Cryopreservation written by Carla Benelli and published by Mdpi AG. This book was released on 2022-01-05 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cryopreservation is the storage of cells, tissues and organs at ultra-low temperatures (-196 °C) and can be an important tool for long-term conservation of plant genetic resources. A wide range of cryogenic procedures have been developed for in vitro explants, dormant buds and non-orthodox seeds in many plant species; however, research that provides novelty, insight and advances for developing and improving new protocols in plant species is still needed. This book includes the development of new protocols, physical and chemical studies related to preservation in liquid nitrogen, and some reviews on several aspects of cryopreservation. This publication will be a useful document for the community of cryopreservation scientists.
Download or read book Cryopreservation of Tropical Plant Germplasm written by Florent Engelmann and published by . This book was released on 2000 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: Development of cryopreservation techniques. Importance of cryopreservation for the conservation of plant genetic resources. Fundamental aspects of cryopreservation. Cryopreservation techniques. Ongoing cryopreservation projects -- Research and its application. Current status of cryopreservation research and future perspectives of its application in national programmes.
Download or read book Managing Global Genetic Resources written by National Research Council and published by National Academies Press. This book was released on 1993-02-01 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: This anchor volume to the series Managing Global Genetic Resources examines the structure that underlies efforts to preserve genetic material, including the worldwide network of genetic collections; the role of biotechnology; and a host of issues that surround management and use. Among the topics explored are in situ versus ex situ conservation, management of very large collections of genetic material, problems of quarantine, the controversy over ownership or copyright of genetic material, and more.
Download or read book Cryopreservation of Musa Germplasm written by Bart Panis and published by Bioversity International. This book was released on 2001 with total page 45 pages. Available in PDF, EPUB and Kindle. Book excerpt: Until 10 years ago, cryopreservation protocols for plant tissues were mainly based on slow freezing in the presence of cryoprotective mixtures containing DMSO (dimthyl slphoxide), sugars, glycerol and/ or proline. Slow freezing results in a freeze-dehydration, leaving less water in the cells to form lethal ice crystals upon exposure to extreme low temperatures.
Download or read book Genetic Preservation of Plant Cells in Vitro written by Brian Grout and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: The long-term storage and maintenance of viable plant cells and organs is an area of active concern across the range of pure and applied plant sciences. In academic, government and commercial laboratories, the extended storage of propagules of one sort or another, with maximum protection of the genome from mutation and altered expression, is often a very necessary activity that can draw heavily on resources and effort. However, preservation per se is typically not an activity in its own right, but a facilitating technology that is part of a larger programme of work. Consequently, there are many laboratories that do not have the benefit of a specialist in storage technology, and have to delegate the responsibility to individuals, or teams, who are faced with a daunting learning curve. To maximise the chances of success, in the shortest possible time and with minimum losses, these researchers need sources of reference that are au thoritative and soundly based in practical experience.
Download or read book Cryopreservation of Musa Germplasm written by Bart Panis and published by Bioversity International. This book was released on 2001-12-01 with total page 45 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cryopreservation of Plant Cells and Organs written by Kartha and published by CRC Press. This book was released on 1985-04-03 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant cell culture. Basic principles pf freezing injury to plant cells: natural tolerance and approaches to cryopreservation. Cryobiology of isolated protoplasts: application to plant cell cryopreservation. Biophysical and ultrastructural studies of membrane alterations in plant cells during extracellular freezing: molecular mechanisms of membrane injury. Cryoprotective compounds in the viable freezing of plant tissues. Meristem culture and germplasm preservation. Cryopreservation of shoot-tips of fruit trees and herbaceous plants. Cryopreservation of potato meristems...
Download or read book Technical Guidelines for the Management of Field and in Vitro Germplasm Collections written by B. M. Reed and published by Bioversity International. This book was released on 2004 with total page 116 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cryopreservation of Tropical Plant Germplasm written by F. Engelmann and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Development of cryopreservation techniques. Importance of cryopreservation for the conservation of plant genetic resources. Fundamental aspects of cryopreservation. Cryopreservation techniques. Ongoing cryopreservation projects -- Research and its application. Current status of cryopreservation research and future perspectives of its application in national programmes.
Download or read book Cryopreservation Biotechnology in Biomedical and Biological Sciences written by Yusuf Bozkurt and published by BoD – Books on Demand. This book was released on 2018-12-12 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cryopreservation has many biotechnological applications in different fields. This has led to an increase in importance of cryobiology as a science that examines the effect of ultra-low temperatures on cells, tissues, organs and organisms and also the freezability of these structures, while maintaining their viability. Nowadays it is well known that this form of biotechnology can be used to solve a lot of problems such as human infertility, life threatening diseases, preservation of gametes and DNA and also biodiversity conservation. Cryopreservation Biotechnology in Biomedical and Biological Sciences describes principles and application of cryopreservation biotechnology in different research areas and includes seven chapters that have been written by experts in their research fields. The chapters included in this book are thought to improve the current understanding of the different areas of using cryopreservation biotechnology.
Download or read book Biotic Diversity and Germplasm Preservation Global Imperatives written by Lloyd Knutson and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 526 pages. Available in PDF, EPUB and Kindle. Book excerpt: Topics for the Beltsville Symposia are selected to highlight specific areas of research and science policy that are of concern to scientists in the Beltsville Area as well as to the general scientific community. Each sympo sium in the series is structured to provide a realistic appraisal of current findings, research progress, and relevant policy issues within the constraints established by the organizing committee. Thus, the presentations and dis cussions that have marked these symposia have had a strong appeal to the broad community of scientists. Knowledge of the diversity of living organisms is still quite limited. Since the time of Linnaeus, about 1.7 million species have been described. The actual number has been estimated between 5-50 million. Many species, land races, and strains are vanishing. Clearly, the world's scientific institutions are inadequately equipped to attain sufficient knowledge of a significant fraction of the diverse living forms. Also, efforts in the collection and preservation of germplasm of plants and animals urgently need to be strengthened. These mattes are critically important to future generations. This symposium addresses vital concerns of biotic diversity and germ plasm preservation from diverse perspectives. Many of the parts provide concrete recommendations for action, and they call attention to areas of research that must be pursued with intensity.
Download or read book Plant Cell Biotechnology written by M. Salome S. Pais and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 493 pages. Available in PDF, EPUB and Kindle. Book excerpt: A number of interdisciplinary fields related to Plant Cell Biotechnology are discussed. The two main directions are: Plant cell culture in agricultural applications for the improvement of crops and industrial applications in the production of secondary metabolites. A number of areas such as physiological and biochemical aspects of autotrophic cells, gene characterization in higher plants, transformation of plant cells, genetic stability in plant cell cultures, somatic hybridization and somatic embryogenesis are treated. Recent knowledge on somaclonal and gametoclonal variation as well as on the obtainment of protoplasts and their use for the isolation and culture of heterocaryons as tools for plant breeding are considered. Furthermore, the knowledge on biomass production in fermentor conditions and the role of immobilization for increased production and scale-up of plant cells are discussed.
