Download or read book Crosslinking Strategies for 3D Bioprinting of Engineered Hydrogels written by Sarah Mei Hull and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: 3D bioprinting has demonstrated potential for spatially patterning cells and materials together to create structures that recapitulate native tissue. By mimicking these structural features, bioprinted constructs can serve as in vitro models for basic research or can be implanted into the body for regenerative medicine applications. However, this technique is limited by a lack of suitable soft materials for use as bioinks since it remains challenging to design materials that are both printable and can support cell culture. In addition, as matrix material properties are known to influence cell phenotype and behavior, it is becoming increasingly important to tailor the bioink's properties to each individual cell type in order to create more complex, functional printed structures. In this thesis, I explore how engineered, tunable hydrogels can be used to address the current limitations of 3D bioprinting and then develop crosslinking strategies to control the mechanical, biochemical, and dynamic behavior of bioink materials before and after printing.
Download or read book Study on Microextrusion based 3D Bioprinting and Bioink Crosslinking Mechanisms written by Liliang Ouyang and published by . This book was released on 2019 with total page 141 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive study on microextrusion-based 3D bioprinting technologies for bioinks with various crosslinking mechanisms, chiefly focusing on the bioprinting process and bioink properties to provide readers with a better understanding of this state-of-the-art technology. Further, it summarizes a number of general criteria and research routes for microextrusion-based 3D bioprinting using three experimental studies based on shear-thinning, thermo-sensitive and non-viscous hydrogel bioinks. The book also presents sample applications in the areas of stem cells and cell matrix interaction. The book highlights pioneering results in the development of bioprinting technologies and bioinks, which were published in high-quality journals such as Advanced Materials, Biofabrication and ACS Biomaterials Science & Engineering. These include an in-situ crosslinking strategy that overcomes the viscosity limits for bioinks, which is virtually impossible using conventional strategies, and can be generalized for other bioink formulations.
Download or read book Injectable Hydrogels for 3D Bioprinting written by Insup Noh and published by Royal Society of Chemistry. This book was released on 2021-07-30 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hydrogels represent one of the cornerstones in tissue engineering and regenerative medicine, due to their biocompatibility and physiologically relevant properties. These inherent characteristics mean that they can be widely exploited as bioinks in 3D bioprinting for tissue engineering applications as well as injectable gels for cell therapy and drug delivery purposes. The research in these fields is booming and this book provides the reader with a terrific introduction to the burgeoning field of injectable hydrogel design, bioprinting and tissue engineering. Edited by three leaders in the field, users of this book will learn about different classes of hydrogels, properties and synthesis strategies to produce bioinks. A section devoted to the key processing and design challenges at the hydrogel/3D bioprinting/tissue interface is also covered. The final section of the book closes with pertinent clinical applications. Tightly edited, the reader will find this book to be a coherent resource to learn from. It will appeal to those working across biomaterials science, chemical and biomedical engineering, tissue engineering and regenerative medicine.
Download or read book Modular Hyaluronan Based Hydrogels for 3D Cell Culture and Bioprinting written by Michael Jury and published by Linköping University Electronic Press. This book was released on 2022-04-29 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: Three-dimensional (3D) cell culture facilitates development of biological relevant assays for drug screening and toxicity testing. Compared to conventional 2D cell culture, cells cultured in 3D can more accurately mimic human tissues and organs and thus provide ex vivo data with potentially better predictive value for cancer research, pharmacology, and toxicology, reducing the need for animal models, improving experimental reproducibility, and reducing time and costs in drug development. The most widely used options for scaffold-based 3D cell culture are, however, based on poorly defined biologically derived extracellular matrix (ECM) with limited possibilities to tailor material properties and that are difficult to combine with state-of-the art biofabrication techniques. The overall aim this thesis was to design and explore modular hyaluronan (HA) based ECM-mimicking hydrogels with tuneable physiochemical properties and biofunctionalities, for development of advanced 3D cell models and biofabrication. The thesis work is presented in five papers. In paper I, we used copper free click chemistry for both hydrogel cross-linking and functionalization with fibronectin derived peptide sequences for culture of human induced pluripotent-derived hepatocytes in a perfused microfluidic system. The tuneable and bioorthogonal cross-linking enabled both retention of high cell viabilities and fabrication of a functional liver-on-chip solution. In paper II, we combined the developed HA-based hydrogel system with homo- and heterodimerizing helix-loop-helix peptides for modulation of both cross-linking density and biofunctionalization. We further demonstrated the possibilities to use these hydrogels as bioinks for 3D bioprinting where both the molecular composition and the physical properties of the printed structures could be dynamically altered, providing new avenues for four-dimensional (4D) bioprinting. In paper III we investigated the possibilities to chemically conjugate full size recombinant human laminin-521 (LN521) in the HA-based hydrogels system using copper-free click chemistry, with the aim to enable 3D culture and 3D bioprinting of neurons. We quantified the impact of using different linkers to tether LN521 and the influence of LN-functionalization on the structural and mechanical properties of the hydrogels. We show that both differentiated and non-differentiated neuroblastoma cells and long-term self-renewing neuroepithelial stem cells (lt-NES) remained viable in the hydrogels. The hydrogels also had a protected effect on lt-NES during syringe ejection and bioprinting. In paper IV, we used HA-based hydrogels modified with peptides sequences derived from fibronectin and laminin for culture of fetal primary astrocytes (FPA). We explored both the interactions between the hydrogels and FPA and possibilities to 3D bioprint FPAs. Finally, in paper V, we developed HA-nanocellulose composite hydrogels with the aim to increase printing fidelity and enable fabrication of multi-layered bioprinted structures without the use of a support bath. In addition to HA, we used wood-fibre derived nanocellulose (NC) to increase the viscosity of the bioink during the printing process. The developed biorthogonal and modular hydrogel systems provide a large degree of flexibility that allows for encapsulation and culture of different cell types and processing using different techniques, which can contribute to further exploration of fabrication of biologically relevant tissue and disease models.
Download or read book Engineered Tissues Using Bioactive Hydrogels written by Di Huang and published by Frontiers Media SA. This book was released on 2022-08-22 with total page 161 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book 3D Bioprinting in Regenerative Engineering written by Ali Khademhosseini and published by CRC Press. This book was released on 2018-04-17 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: Regenerative engineering is the convergence of developmental biology, stem cell science and engineering, materials science, and clinical translation to provide tissue patches or constructs for diseased or damaged organs. Various methods have been introduced to create tissue constructs with clinically relevant dimensions. Among such methods, 3D bioprinting provides the versatility, speed and control over location and dimensions of the deposited structures. Three-dimensional bioprinting has leveraged the momentum in printing and tissue engineering technologies and has emerged as a versatile method of fabricating tissue blocks and patches. The flexibility of the system lies in the fact that numerous biomaterials encapsulated with living cells can be printed. This book contains an extensive collection of papers by world-renowned experts in 3D bioprinting. In addition to providing entry-level knowledge about bioprinting, the authors delve into the latest advances in this technology. Furthermore, details are included about the different technologies used in bioprinting. In addition to the equipment for bioprinting, the book also describes the different biomaterials and cells used in these approaches. This text: Presents the principles and applications of bioprinting Discusses bioinks for 3D printing Explores applications of extrusion bioprinting, including past, present, and future challenges Includes discussion on 4D Bioprinting in terms of mechanisms and applications
Download or read book Designing Bioinks for 3D Bioprinting in Gel based Support Baths written by Christopher Dean Lindsay and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: 3D bioprinting has emerged as a powerful tool to pattern extracellular matrix (ECM) and living cells to produce biological structures that mimic native tissues and organs ex vivo. Bioinks, the cell- and ECM-laden mixtures used during bioprinting, have primarily been composed of biopolymers appropriated from traditional 3D tissue engineering to form hydrogels following printing. These materials are not particularly well suited to meet the challenges introduced through bioprinting, namely, cell sedimentation, mechanical damage while printing, printing in gel-based support baths, and tunability of the mechanics and bioactivity of the biopolymer hydrogels. New bioink materials can be produced through intentional design of the crosslinking scheme of the hydrogel network before and after bioprinting into a gel-based support bath. The work presented in this thesis explores the benefits of designing new bioink materials for 3D bioprinting that move beyond the simplest crosslinking strategies used in traditional tissue engineering materials. Specifically, bioinks that employ two different stages of crosslinking, a first-stage crosslinking before the material is printed and a second stage following printing in a bath, prove to be effective for 3D bioprinting. One example of the increased versatility of bioinks from a dual-crosslinking approach is a bioprinted stem cell expansion lattice for stem cell manufacturing made using a traditional bioink material, sodium alginate. The second half of this thesis introduces two new crosslinking schemes that are made possible only by bioprinting into a gel-based support bath. Both bioorthogonal and hydrazone dynamic covalent chemistries are explored for producing new bioinks. The work put forward in this thesis is a step toward designing new bioinks for 3D bioprinting. An array of crosslinking schemes for bioinks are presented alongside creative uses of existing biopolymers to produce more adaptable bioinks.
Download or read book Determining the Effect of Structure and Function on 3D Bioprinted Hydrogel Scaffolds for Applications in Tissue Engineering written by Brent Godau and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of tissue engineering has grown immensely since its inception in the late 1980s. However, currently commercialized tissue engineered products are simple in structure. This is due to a pre-clinical bottleneck in which complex tissues are unable to be fabricated. 3D bioprinting has become a versatile tool in engineering complex tissues and offers a solution to this bottleneck. Characterizing the mechanical properties of engineered tissue constructs provides powerful insight into the viability of engineered tissues for their desired application. Current methods of mechanical characterization of soft hydrogel materials used in tissue engineering destroy the sample and ignore the effect of 3D bioprinting on the overall mechanical properties of a construct. Herein, this work reports on the novel use of a non-destructive method of viscoelastic analysis to demonstrate the influence of 3D bioprinting strategy on mechanical properties of hydrogel tissue scaffolds. 3D bioprinting is demonstrated as a versatile tool with the ability to control mechanical and physical properties. Structure-function relationships are developed for common 3D bioprinting parameters such as printed fiber size, printed scaffold pattern, and bioink formulation. Further studies include effective real-time monitoring of crosslinking, and mechanical characterization of multi-material scaffolds. We envision this method of characterization opening a new wave of understanding and strategy in tissue engineering.
Download or read book Cell Assembly with 3D Bioprinting written by Yong He and published by John Wiley & Sons. This book was released on 2022-03-14 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides an up-to-date outline of cell assembly methods and applications of 3D bioprinting Cell Assembly with 3D Bioprinting provides an accesible overview of the layer-by-layer manufacturing of living structures using biomaterials. Focusing on technical implemention in medical and bioengineering applications, this practical guide summarize each key aspect of the 3D bioprinting process. Contributions from a team of leading researchers describe bioink preparation, printing method selection, experimental protocols, integration with specific applications, and more. Detailed, highly illustrated chapters cover different bioprinting approaches and their applications, including coaxial bioprinting, digital light projection, direct ink writing, liquid support bath-assisted 3D printing, and microgel-, microfiber-, and microfluidics-based biofabrication. The book includes practical examples of 3D bioprinting, a protocol for typical 3D bioprinting, and relevant experimental data drawn from recent research. * Highlights the interdisciplinary nature of 3D bioprinting and its applications in biology, medicine, and pharmaceutical science * Summarizes a variety of commonly used 3D bioprinting methods * Describes the design and preparation of various types of bioinks * Discusses applications of 3D bioprinting such as organ development, toxicological research, clinical transplantation, and tissue repair Covering a wide range of topics, Cell Assembly with 3D Bioprinting is essential reading for advanced students, academic researchers, and industry professionals in fields including biomedicine, tissue engineering, bioengineering, drug development, pharmacology, bioglogical screening, and mechanical engineering.
Download or read book Bioprinting in Regenerative Medicine written by Kursad Turksen and published by Springer. This book was released on 2015-09-02 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents the current state of laser-assisted bioprinting, a cutting edge tissue engineering technology. Nineteen chapters discuss the most recent developments in using this technology for engineering different types of tissue. Beginning with an overview, the discussion covers bioprinting in cell viability and pattern viability, tissue microfabrication to study cell proliferation, microenvironment for controlling stem cell fate, cell differentiation, zigzag cellular tubes, cartilage tissue engineering, osteogenesis, vessel substitutes, skin tissue and much more. Because bioprinting is on its way to becoming a dominant technology in tissue-engineering, Bioprinting in Regenerative Medicine is essential reading for those researching or working in regenerative medicine, tissue engineering or translational research. Those studying or working with stem cells who are interested in the development of the field will also find the information invaluable.
Download or read book 3D Bioprinting written by Dong-Woo Cho and published by Springer Nature. This book was released on 2019-12-04 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text advances fundamental knowledge in modeling in vitro tissues/organs as an alternative to 2D cell culture and animal testing. Prior to engineering in vitro tissues/organs,the descriptions of prerequisites (from pre-processing to post-processing) in modeling in vitro tissues/organs are discussed. The most prevalent technologies that have been widely used for establishing the in vitro tissue/organ models are also described, including transwell, cell spheroids/sheets, organoids, and microfluidic-based chips. In particular, the authors focus on 3D bioprinting in vitro tissue/organ models using tissue-specific bioinks. Several representative bioprinting methods and conventional bioinks are introduced. As a bioink source, decellularized extracellular matrix (dECM) are importantly covered, including decellularization methods, evaluation methods for demonstrating successful decellularization, and material safety. Taken together, the authors delineate various application examples of 3D bioprinted in vitro tissue/organ models especially using dECM bioinks.
Download or read book Frontiers in Tissue Engineering written by C.W. Patrick and published by Elsevier. This book was released on 1998-02-20 with total page 717 pages. Available in PDF, EPUB and Kindle. Book excerpt: Frontiers in Tissue Engineering is a carefully edited compilation of state-of-the-art contributions from an international authorship of experts in the diverse subjects that make up tissue engineering. A broad representation of the medical, scientific, industrial and regulatory community is detailed in the book. The work is an authoritative and comprehensive reference source for scientists and clinicians working in this emerging field. The book is divided into three parts: fundamentals and methods of tissue engineering, tissue engineering applied to specialised tissues, and tissue engineering applied to organs. The text offers many novel approaches, including a detailed coverage of cell-tissue interactions at cellular and molecular levels; cell-tissue surface, biochemical, and mechanical environments; biomaterials; engineering design; tissue-organ function; new approaches to tissue-organ regeneration and replacement of function; ethical considerations of tissue engineering; and government regulation of tissue-engineered products.
Download or read book Injectable Hydrogels for 3D Bioprinting written by Insup Noh and published by Royal Society of Chemistry. This book was released on 2021-07-23 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hydrogels represent one of the cornerstones in tissue engineering and regenerative medicine, due to their biocompatibility and physiologically relevant properties. These inherent characteristics mean that they can be widely exploited as bioinks in 3D bioprinting for tissue engineering applications as well as injectable gels for cell therapy and drug delivery purposes. The research in these fields is booming and this book provides the reader with a terrific introduction to the burgeoning field of injectable hydrogel design, bioprinting and tissue engineering. Edited by three leaders in the field, users of this book will learn about different classes of hydrogels, properties and synthesis strategies to produce bioinks. A section devoted to the key processing and design challenges at the hydrogel/3D bioprinting/tissue interface is also covered. The final section of the book closes with pertinent clinical applications. Tightly edited, the reader will find this book to be a coherent resource to learn from. It will appeal to those working across biomaterials science, chemical and biomedical engineering, tissue engineering and regenerative medicine.
Download or read book Essentials of 3D Biofabrication and Translation written by Anthony Atala and published by Academic Press. This book was released on 2015-07-17 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: Essentials of 3D Biofabrication and Translation discusses the techniques that are making bioprinting a viable alternative in regenerative medicine. The book runs the gamut of topics related to the subject, including hydrogels and polymers, nanotechnology, toxicity testing, and drug screening platforms, also introducing current applications in the cardiac, skeletal, and nervous systems, and organ construction. Leaders in clinical medicine and translational science provide a global perspective of the transformative nature of this field, including the use of cells, biomaterials, and macromolecules to create basic building blocks of tissues and organs, all of which are driving the field of biofabrication to transform regenerative medicine. Provides a new and versatile method to fabricating living tissue Discusses future applications for 3D bioprinting technologies, including use in the cardiac, skeletal, and nervous systems, and organ construction Describes current approaches and future challenges for translational science Runs the gamut of topics related to the subject, from hydrogels and polymers to nanotechnology, toxicity testing, and drug screening platforms
Download or read book 3D Printing and Biofabrication written by Aleksandr Ovsianikov and published by Springer. This book was released on 2018-06-08 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an in-depth introduction to 3D printing and biofabrication and covers the recent advances in additive manufacturing for tissue engineering. The book is divided into two parts, the first part on 3D printing discusses conventional approaches in additive manufacturing aimed at fabrication of structures, which are seeded with cells in a subsequent step. The second part on biofabrication presents processes which integrate living cells into the fabrication process.
Download or read book Biomedical Hydrogels written by Steve Rimmer and published by Elsevier. This book was released on 2011-02-26 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hydrogels are very important for biomedical applications because they can be chemically manipulated to alter and control the hydrogel’s interaction with cells and tissues. Their flexibility and high water content is similar to that of natural tissue, making them extremely suitable for biomaterials applications. Biomedical hydrogels explores the diverse range and use of hydrogels, focusing on processing methods and novel applications in the field of implants and prostheses. Part one of this book concentrates on the processing of hydrogels, covering hydrogel swelling behaviour, superabsorbent cellulose-based hydrogels and regulation of novel hydrogel products, as well as chapters focusing on the structure and properties of hydrogels and different fabrication technologies. Part two covers existing and novel applications of hydrogels, including chapters on spinal disc and cartilage replacement implants, hydrogels for ophthalmic prostheses and hydrogels for wound healing applications. The role of hydrogels in imaging implants in situ is also discussed. With its distinguished editor and international team of contributors, Biomedical hydrogels is an excellent reference for biomedical research scientists and engineers in industry and academia, as well as others involved in research in this area, such as research clinicians. Examines the diverse range and use of hydrogels, focusing on processing methods and novel applications Comprehensive book explores the structure and properties of hydrogels and different fabrication technologies Covers important areas such as processing of hydrogels, covering hydrogel swelling behaviour, superabsorbent cellulose-based hydrogels and regulation of novel hydrogel products
Download or read book Hydrogel for Biomedical Applications written by Andy H. Choi and published by Springer Nature. This book was released on with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt:
