Download or read book Contrast Techniques in Light Microscopy written by S. Bradbury and P.J. Evennett and published by Garland Science. This book was released on 2020-08-26 with total page 195 pages. Available in PDF, EPUB and Kindle. Book excerpt: Contrast in an image is essential to distinguish features from one another and from the background. This practical handbook describes the ways in which light interacts with the specimen in the microscope.
Download or read book Light and Video Microscopy written by Randy O. Wayne and published by Academic Press. This book was released on 2013-12-16 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation. Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope Clearly develops all ideas from historical and logical foundations Laboratory exercises included to assist the reader with practical applications Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope
Download or read book Introduction to Optical Microscopy written by Jerome Mertz and published by Cambridge University Press. This book was released on 2019-08 with total page 475 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.
Download or read book Essentials of Polarized Light Microscopy and Ancillary Techniques written by John Gustav Delly and published by . This book was released on 2019-09-09 with total page 702 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual of essentials details the setup and operation of a polarized light microscope for microscopic particle characterization and identification using an approach to applied polarized light microscopy required for applications including industrial problem solving, contaminant identification, trace evidence analysis, and much more.
Download or read book Understanding Light Microscopy written by Jeremy Sanderson and published by John Wiley & Sons. This book was released on 2019-03-28 with total page 848 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.
Download or read book Fundamentals of Light Microscopy and Electronic Imaging written by Douglas B. Murphy and published by . This book was released on 2013 with total page 538 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Applied Polymer Light Microscopy written by Derek A. Hemsley and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthetic polymers make excellent specimens for light microscopy. Despite this, the use of the technique, at least in its advanced forms, is not so widespread as might be expected. Although reliable and relevant data are difficult to find and quantify, it seems that in other fields of materials science and technology there is a greater readiness to tum to the microscope in research, in industrial problem solving, or for quality assessment and control. It also seems that the reasons for the present situation are partly historical, partly the result of the structure of the plastics and rubber industries, and partly the education and training background of senior staff who tend to be chemistry or engineering based. In neither field does light microscopy feature strongly in the basic training. The primary aim of this book is to provide some insight into the range oflight microscopy techniques applicable to polymeric specimens, and to highlight typical applications to commercial polymers and polymer products. Where appropriate, the optical techniques involved are discussed in some detail. However, it has not been the intention to produce a light microscopy textbook dealing with the principles and design of the basic instrument. Many such texts are available, and selected examples are cited in the reference list at the end of most chapters.
Download or read book Microscopy Techniques written by Jens Rietdorf and published by Springer Science & Business Media. This book was released on 2005-06-23 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions by numerous experts
Download or read book Cell Imaging Techniques written by Douglas J. Taatjes and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.
Download or read book CONTRACT TECHNIQUES LIGHT written by BRADBURY and published by Taylor & Francis. This book was released on 1996-05-31 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text describes the interactions of light and the specimen in the microscope in relation to the manner in which they are used to generate contrast in the image of a specimen. Chapters cover bright-field and dark-ground techniques for both transmitted a
Download or read book Urine Sediment written by Josefine Neuendorf and published by Springer Nature. This book was released on 2020-06-17 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a comprehensive resource and up-to-date description of all urinary sediment constituents which are presented in bright-field mode and in phase-contrast mode. Thanks to numerous detailed images of urinary sediment constituents, the reader is able to easily compare what they view microscopically with high-resolution photographs and short films. The book is also designed to aid the identification of rare urine constituents in their native state without prior staining. It also features guidance on how set-up a microscope , microscopy techniques, and preanalytics. Exercises focused on microscopic analysis and diagnosis and a urinary sediment quiz reinforce key concepts to aid learning. Urine Sediment provides a practically applicable guide to the recognition of urinary sediment constituents. It is therefore a critical resource for trainees and experienced practitioners in urology, nephrology, gynecology and general practice who need to be able to accurately and quickly identify urine sediment constituents.
Download or read book Coherent Light Microscopy written by Pietro Ferraro and published by Springer Science & Business Media. This book was released on 2011-02-09 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book deals with the latest achievements in the field of optical coherent microscopy. While many other books exist on microscopy and imaging, this book provides a unique resource dedicated solely to this subject. Similarly, many books describe applications of holography, interferometry and speckle to metrology but do not focus on their use for microscopy. The coherent light microscopy reference provided here does not focus on the experimental mechanics of such techniques but instead is meant to provide a users manual to illustrate the strengths and capabilities of developing techniques. The areas of application of this technique are in biomedicine, medicine, life sciences, nanotechnology and materials sciences.
Download or read book Imaging Neurons written by Rafael Yuste and published by . This book was released on 2000 with total page 848 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past decade, advances in microscopy have been coupled with new methods of culturing and labeling cells to generate the new science of imaging. Imaging technologies allow investigators to look directly inside living cells and probe their form and function in unprecedented detail. This approach is revolutionizing many aspects of biomedical research, particularly neuroscience, in which visual techniques have traditionally been so important. This manual is the first comprehensive description of the range of imaging technologies being applied to living cells. With its origins in a laboratory course taught at Cold Spring Harbor Laboratory by the editors and contributors, it is packed with the kind of technical detail and practical advice that are essential for success, yet seldom found in the research literature. It covers both established methods and cutting-edge techniques such as multiphoton excitation microscopy and imaging of genetically engineered probes. Although it is neurons to which these technologies are most commonly applied, the methods described are readily adaptable to many other cell types. This book will therefore be an invaluable aid to investigators in cell and developmental biology and immunology as well as neuroscience who wish to take advantage of the extraordinary insights into cellular function offered by imaging technologies.
Download or read book Special Methods in Light Microscopy written by Robert B. McLaughlin and published by . This book was released on 1977 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Generalized Phase Contrast written by Jesper Glückstad and published by Springer. This book was released on 2009-08-20 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: Generalized Phase Contrast elevates the phase contrast technique not only to improve phase imaging but also to cross over and interface with diverse and seemingly disparate fields of contemporary optics and photonics. This book presents a comprehensive introduction to the Generalized Phase Contrast (GPC) method including an overview of the range of current and potential applications of GPC in wavefront sensing and phase imaging, structured laser illumination and image projection, optical trapping and manipulation, and optical encryption and decryption. The GPC method goes further than the restrictive assumptions of conventional Zernike phase contrast analysis and achieves an expanded range of validity beyond weak phase perturbations. The generalized analysis yields design criteria for tuning experimental parameters to achieve optimal performance in terms of accuracy, fidelity and light efficiency. Optimization can address practical issues, such as finding an optimal spatial filter for the chosen application, and can even enable a Reverse Phase Contrast mode where intensity patterns are converted into a phase modulation.
Download or read book Materials Characterization written by Yang Leng and published by John Wiley & Sons. This book was released on 2009-03-04 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers state-of-the-art techniques commonly used in modern materials characterization. Two important aspects of characterization, materials structures and chemical analysis, are included. Widely used techniques, such as metallography (light microscopy), X-ray diffraction, transmission and scanning electron microscopy, are described. In addition, the book introduces advanced techniques, including scanning probe microscopy. The second half of the book accordingly presents techniques such as X-ray energy dispersive spectroscopy (commonly equipped in the scanning electron microscope), fluorescence X-ray spectroscopy, and popular surface analysis techniques (XPS and SIMS). Finally, vibrational spectroscopy (FTIR and Raman) and thermal analysis are also covered.
Download or read book Light Microscopy in Biology written by Alan J. Lacey and published by Oxford University Press, USA. This book was released on 1999 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first edition of Light Microscopy in Biology: A Practical approach was published, techniques in modern light microscopy have improved considerably. This fully updated edition includes revised topics from the first edition as well as coverage of techniques and technologies that havebeen developed since it was published. As before, the book starts with an explanation of the basic techniques, and goes on to describe current methods in: chromosome microscopy, immunohistochemistry, fluorescence microscopy, image building and video microscopy. Totally new topics covered include:confocal microscopy, calcium and pH imaging, microinjection techniques and nanovid microscopy. There are also whole chapters now devoted to reflection contrast microscopy and histomorphometry. This new edition will be of great interest to postgraduate and postdoctoral researchers in biomedicineand cell biology - both those experienced with light microscopic techniques and newcomers to the field.
