Download or read book Continuous Cultures Of Cells written by Pete H. Calcott and published by CRC Press. This book was released on 2020-03-26 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: First Published in 1981, this book offers a full insight into the development of cell cultures. Carefully compiled and filled with a vast repertoire of notes, diagrams, and references this book serves as a useful reference for students of microbiology and other practitioners in their respective fields.
Download or read book Continuous Cultures of Cells written by Peter H. Calcott and published by CRC Press. This book was released on 2017-07-28 with total page 209 pages. Available in PDF, EPUB and Kindle. Book excerpt: This two-volume set will be useful to the established continuous culture operator, as well as the researcher, teacher, and student who is interested in learning how the technique of continuous culture could be useful in answering both basic and applied questions in microbiology and cell biology.
Download or read book Introduction to Research with Continuous Cultures written by Herbert Ernest Kubitschek and published by Prentice Hall. This book was released on 1970 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Polymicrobial Diseases written by Kim A. Brogden and published by . This book was released on 2002 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Polymicrobial diseases, those involving more than one etiologic agent, are more common than is generally realized and include respiratory diseases, gastroenteritis, conjunctivitis, keratitis, hepatitis, periodontal diseases, multiple sclerosis, genital infections, intra -- abdominal infections, and pertussis.
Download or read book Continuous Cultures of Cells written by and published by . This book was released on 1981 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Culture Engineering written by Gyun Min Lee and published by John Wiley & Sons. This book was released on 2020-01-13 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers a comprehensive overview of cell culture engineering, providing insight into cell engineering, systems biology approaches and processing technology In Cell Culture Engineering: Recombinant Protein Production, editors Gyun Min Lee and Helene Faustrup Kildegaard assemble top class authors to present expert coverage of topics such as: cell line development for therapeutic protein production; development of a transient gene expression upstream platform; and CHO synthetic biology. They provide readers with everything they need to know about enhancing product and bioprocess attributes using genome-scale models of CHO metabolism; omics data and mammalian systems biotechnology; perfusion culture; and much more. This all-new, up-to-date reference covers all of the important aspects of cell culture engineering, including cell engineering, system biology approaches, and processing technology. It describes the challenges in cell line development and cell engineering, e.g. via gene editing tools like CRISPR/Cas9 and with the aim to engineer glycosylation patterns. Furthermore, it gives an overview about synthetic biology approaches applied to cell culture engineering and elaborates the use of CHO cells as common cell line for protein production. In addition, the book discusses the most important aspects of production processes, including cell culture media, batch, fed-batch, and perfusion processes as well as process analytical technology, quality by design, and scale down models. -Covers key elements of cell culture engineering applied to the production of recombinant proteins for therapeutic use -Focuses on mammalian and animal cells to help highlight synthetic and systems biology approaches to cell culture engineering, exemplified by the widely used CHO cell line -Part of the renowned "Advanced Biotechnology" book series Cell Culture Engineering: Recombinant Protein Production will appeal to biotechnologists, bioengineers, life scientists, chemical engineers, and PhD students in the life sciences.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Tissue and Cell Culture written by Herbert Edward Street and published by Univ of California Press. This book was released on 1973 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Impact of Food Bioactives on Health written by Kitty Verhoeckx and published by Springer. This book was released on 2015-04-29 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: “Infogest” (Improving Health Properties of Food by Sharing our Knowledge on the Digestive Process) is an EU COST action/network in the domain of Food and Agriculture that will last for 4 years from April 4, 2011. Infogest aims at building an open international network of institutes undertaking multidisciplinary basic research on food digestion gathering scientists from different origins (food scientists, gut physiologists, nutritionists...). The network gathers 70 partners from academia, corresponding to a total of 29 countries. The three main scientific goals are: Identify the beneficial food components released in the gut during digestion; Support the effect of beneficial food components on human health; Promote harmonization of currently used digestion models Infogest meetings highlighted the need for a publication that would provide researchers with an insight into the advantages and disadvantages associated with the use of respective in vitro and ex vivo assays to evaluate the effects of foods and food bioactives on health. Such assays are particularly important in situations where a large number of foods/bioactives need to be screened rapidly and in a cost effective manner in order to ultimately identify lead foods/bioactives that can be the subject of in vivo assays. The book is an asset to researchers wishing to study the health benefits of their foods and food bioactives of interest and highlights which in vitro/ex vivo assays are of greatest relevance to their goals, what sort of outputs/data can be generated and, as noted above, highlight the strengths and weaknesses of the various assays. It is also an important resource for undergraduate students in the ‘food and health’ arena.
Download or read book The Dynamics of Continuous Culture with the Recycle of Cells written by K. M. E. Wagi-Alla and published by . This book was released on 1973 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Culturing Life written by Hannah Landecker and published by Harvard University Press. This book was released on 2010-03-30 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: How did cells make the journey, one we take so much for granted, from their origin in living bodies to something that can be grown and manipulated on artificial media in the laboratory, a substantial biomass living outside a human body, plant, or animal? This is the question at the heart of Hannah Landecker's book. She shows how cell culture changed the way we think about such central questions of the human condition as individuality, hybridity, and even immortality and asks what it means that we can remove cells from the spatial and temporal constraints of the body and "harness them to human intention." Rather than focus on single discrete biotechnologies and their stories--embryonic stem cells, transgenic animals--Landecker documents and explores the wider genre of technique behind artificial forms of cellular life. She traces the lab culture common to all those stories, asking where it came from and what it means to our understanding of life, technology, and the increasingly blurry boundary between them. The technical culture of cells has transformed the meaning of the term "biological," as life becomes disembodied, distributed widely in space and time. Once we have a more specific grasp on how altering biology changes what it is to be biological, Landecker argues, we may be more prepared to answer the social questions that biotechnology is raising.
Download or read book Cell Culture Engineering written by Wei-Shu Hu and published by Springer. This book was released on 2006-08-16 with total page 179 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the introduction of recombinant human growth hormone and insulin a quarter century ago, protein therapeutics has greatly broadened the ho- zon of health care. Many patients suffering with life-threatening diseases or chronic dysfunctions, which were medically untreatable not long ago, can attest to the wonder these drugs have achieved. Although the ?rst generation of p- tein therapeutics was produced in recombinant Escherichia coli, most recent products use mammalian cells as production hosts. Not long after the ?rst p- duction of recombinant proteins in E. coli, it was realized that the complex tasks of most post-translational modi?cations on proteins could only be ef?ciently carried out in mammalian cells. In the 1990s, we witnessed a rapid expansion of mammalian-cell-derived protein therapeutics, chie?y antibodies. In fact, it has been nearly a decade since the market value of mammalian-cell-derived protein therapeutics surpassed that of those produced from E. coli. A common characteristic of recent antibody products is the relatively large dose required for effective therapy, demanding larger quantities for the treatment of a given disease. This, coupled with the broadening repertoire of protein drugs, has rapidly expanded the quantity needed for clinical applications. The increasing demand for protein therapeutics has not been met exclusively by construction of new manufacturing plants and increasing total volume capacity. More - portantly the productivity of cell culture processes has been driven upward by an order of magnitude in the past decade.
Download or read book Bioprocess Engineering Principles written by Pauline M. Doran and published by Elsevier. This book was released on 1995-04-03 with total page 455 pages. Available in PDF, EPUB and Kindle. Book excerpt: The emergence and refinement of techniques in molecular biology has changed our perceptions of medicine, agriculture and environmental management. Scientific breakthroughs in gene expression, protein engineering and cell fusion are being translated by a strengthening biotechnology industry into revolutionary new products and services. Many a student has been enticed by the promise of biotechnology and the excitement of being near the cutting edge of scientific advancement. However, graduates trained in molecular biology and cell manipulation soon realise that these techniques are only part of the picture. Reaping the full benefits of biotechnology requires manufacturing capability involving the large-scale processing of biological material. Increasingly, biotechnologists are being employed by companies to work in co-operation with chemical engineers to achieve pragmatic commercial goals. For many years aspects of biochemistry and molecular genetics have been included in chemical engineering curricula, yet there has been little attempt until recently to teach aspects of engineering applicable to process design to biotechnologists.This textbook is the first to present the principles of bioprocess engineering in a way that is accessible to biological scientists. Other texts on bioprocess engineering currently available assume that the reader already has engineering training. On the other hand, chemical engineering textbooks do not consider examples from bioprocessing, and are written almost exclusively with the petroleum and chemical industries in mind. This publication explains process analysis from an engineering point of view, but refers exclusively to the treatment of biological systems. Over 170 problems and worked examples encompass a wide range of applications, including recombinant cells, plant and animal cell cultures, immobilised catalysts as well as traditional fermentation systems.* * First book to present the principles of bioprocess engineering in a way that is accessible to biological scientists* Explains process analysis from an engineering point of view, but uses worked examples relating to biological systems* Comprehensive, single-authored* 170 problems and worked examples encompass a wide range of applications, involving recombinant plant and animal cell cultures, immobilized catalysts, and traditional fermentation systems* 13 chapters, organized according to engineering sub-disciplines, are groupled in four sections - Introduction, Material and Energy Balances, Physical Processes, and Reactions and Reactors* Each chapter includes a set of problems and exercises for the student, key references, and a list of suggestions for further reading* Includes useful appendices, detailing conversion factors, physical and chemical property data, steam tables, mathematical rules, and a list of symbols used* Suitable for course adoption - follows closely curricula used on most bioprocessing and process biotechnology courses at senior undergraduate and graduate levels.
Download or read book Animal Cells Culture and Media written by D. C. Darling and published by Wiley-Blackwell. This book was released on 1994 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference guide covers the fundamentals of animal cell cultures. It includes details of culture media, supplements, culture vessels, standard cell lines, passaging, cell separation techniques, cryopreservation, transfection, cell cloning and the creation of cell lines.
Download or read book Continuous Cultivation of Microorganisms written by I. Malek and published by Elsevier. This book was released on 2012-12-02 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: Continuous Cultivation of Microorganisms deals with research studies on the continuous processes in cultivation and their applications in industrial production. Divided into 40 chapters, the book is composed of contributions of authors who have conducted extensive studies on continuous cultivation. The discussions start with situations and trends in the advancement of continuous cultivation, multi-stage continuous culture, the theory of multi-stage continuous cultures, and a stochastic model of continuous cultivation of microorganisms in several vessels. This discussion is followed by the presentation of the theory of continuous fermentation; the use of continuous culture method for analyzing cell functions; and a comparative study of cell mass production in a single and multistage cultivation. The book goes further with discussions on soil microbiology and the processes and environments involved in the culture of bacteria. The text also presents the fermentation processes of beer, lactic acid, alcohol, and liquor. The discussions are supported by methods, experiments, and numerical representations. The book also discusses electronic programs or automatic controls involved in fermentation processes. The selection is a vital source of data for those who are interested in conducting research on continuous cultivation.
Download or read book Introduction to Cell and Tissue Culture written by Jennie P. Mather and published by Springer Science & Business Media. This book was released on 2007-08-20 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.
Download or read book Microscale Controlled Continuous Cell Culture written by Kevin Shao-Kwan Lee and published by . This book was released on 2011 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt: Measurements of metabolic and cellular activity through substrate and product interactions are highly dependent on environmental conditions and cellular metabolic state. For such experiments to be feasible, continuous cultures are utilized to ensure consistent conditions. However, since medium must be replenished every cell doubling time, costs can be prohibitive in large reactors. An integrated microscale bioreactor with built-in fluid metering and environmental control will enable programmed experiments capable of generating reproducible data routinely. This work develops an instrument capable of supporting automated microscale continuous culture experiments. The instrument consists of a plastic-PDMS device capable of continuous flow reactions without volume drift. A novel bonding process is invented to fabricate devices with chemically stable interfaces against water, acids, and bases. We introduce a direct CNC machining and chemical bonding fabrication process for production of fluidic devices with a 1 mL working volume, high oxygen transfer rate (kLa ~ 0.025 s-1), fast mixing (2 s), accurate flow control (± 18 nL), and closed loop control over temperature, cell density, oxygen, and pH. Providing control over environmental parameters allows the system to perform different types of cell culture on a single device, such as batch, fed-batch, chemostat, and turbidostat continuous culture. Validation experiments demonstrate that cells can be grown to high optical densities (OD = 50) and production of commercially relevant chemicals such as DNA vaccines are comparable to large scale bench fermentations. Continuous cultures are also demonstrated without contamination for 3 weeks in a single device and both steady state and dynamically controlled conditions are possible, allowing observations of cell metabolic dynamics.
