Download or read book Comparison of Spectrofluorimetry and Capillary Electrophoresis laser induced Fluorescence for Detection of Fluorescent Dyes in Ground Water written by Andrew H. Grange and published by . This book was released on with total page 1 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Journal of Capillary Electrophoresis written by and published by . This book was released on 1996 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Analysis by Capillary Electrophoresis with Laser induced Fluorescence Detection written by Tara Lee Massie and published by . This book was released on 2010 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) is a versatile and sensitive analytical tool with many potential applications. Its advantages over other chromatographic methods include minimal solvent and sample requirements, low waste volumes, simple instrumentation, and diversity of analytes studied. CE is one of the most efficient separation techniques available for the analysis of both large and small molecules, but because few analytes are natively fluorescent, it is necessary to bind them with fluorescent tags if LIF detection is to be employed. These tags increase sensitivity and provide additional separation from components of the sample that do not bind the tags. In this dissertation, four applications of CE-LIF for the determination of tagged analytes are documented. First, a rapid CE-LIF method was developed for the determination of gamma-aminobutyric acid (GABA) and other important brain biogenic amines and amino acids present in biological samples at low levels. The high efficiency and sensitivity necessary to identify toxicologically relevant changes in neurotransmitter levels in brain tissue and microdialysates following Mn exposure was achieved, and the method required minimum sample work-up and quantities of sample for analysis. A linear relationship between the concentration of GABA and the CE-LIF peak height was established, with correlation coefficients of 0.989 or better for four neurotransmitters. Second, the use of two squarylium-based fluorescent dyes (bis-SQHN-4d and SQHN-3c) as noncovalent protein labels was investigated. The fluorescence emissions of both dyes were enhanced upon complexation with model proteins, demonstrating promise as noncovalent fluorescent protein labels. Third, the nature of interactions between two boronic acid-based probes (SQTM-BA1 and ANQW-BA1) and two model proteins (bovine serum albumin (BSA) and hemagglutinin (HA)) have been explored. The complex between SQTM-BA1 and BSA was the only complex to show increased fluorescence emission, and the role of electrostatic interactions between SQTM-BA1 and BSA in complex formation was explored. Lastly, CE-LIF methods were developed to label and quantify turnip yellow mosaic virus (TYMV). Noncovalent labels Red-1c and NN127 effectively labeled the surface proteins of the virus, and the labeled viruses were then analyzed by CE-LIF. The CE-LIF methods developed herein for the analysis of neurotransmitters, free solution proteins, and viruses demonstrate the versatility of the technique, especially when coupled with fluorescent labeling.
Download or read book Capillary Electrophoresis Equipped with Laser Induced Fluorescence written by Shokoufeh Ahmadi and published by . This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of this research was to evaluate the feasibility of capillary electrophoresis in micellar electrokinetic mode (MEKC) equipped with laser induced fluorescence (LIF) as a novel technique to study dyes extracted from valuable textiles. CE identification of dyes requires extraction of dyes from the substrate, which is one of the most challenging aspects in conservation science. Therefore, developing a technique with minimum destructive effect on objects is favorable. The most important advantages of using LIF are the low detection limits and high sensitivity which allow for the separation of very small quantities of sample, which makes it suitable for working with historical objects. Feasibility studies were performed to explore separation properties of a small number of historical dyes (alizarin, purpurin, carmine and morin) using: different concentrations of modifiers and borate buffer at different pH values, and applied voltages. By manipulating separation parameters the optimum separation condition for a mixture of 10 dyes was defined as: under 22 kV, 20 mM borate buffer at pH = 8.5; 20 mM SDS with 5% acetonitrile. The separation of flavonols and purpurin using CE at pH>9.2 appeared to be complicated by reproducibility in peak height and peak shape of purpurin. In this study the poor peak reproducibility of CE separations was attributed to photochemical properties of dyes. The 1st order rate constants ( for the decomposition of purpurin and selected flavonol dyes were determined under different pH conditions, for purpurin at pH=9.0 under dark condition was The stability of these dyes was greatly affected by pH, a modest decrease in pH (pH = 8.5) improved the stability and peak reproducibility of these dyes. To extract dyes from textiles, the existing extraction methods with focus on Hanson solubility parameters were evaluated. It appeared that methanol was the most suitable solvent and the dyes in the following samples were extracted by methanol and identified running CE-LIF: textiles from "Salt Man" (600 BC), Safavid dynasty and Chinese robe (1700 AD). CE-LIF was used successfully for separation and identification of small quantities of natural dyes extracted from less than 1 mg samples such as wool, silk and paper.
Download or read book American Laboratory written by and published by . This book was released on 1999 with total page 1106 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Applications of Capillary Electrophoresis and Laser induced Fluorescence Detection to the Analysis of Trace Species written by and published by . This book was released on 1995 with total page 52 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Ph. D. Thesis describes several separation and detection schemes for the analysis of small volume and amount of samples, such as intracellular components and single enzymes developed during research. Indirect Laser-induced fluorescence detection and capillary electrophoresis were used to quantify lactate and pyruvate in single red blood cells. The assay of specific enzyme activities was achieved by monitoring the highly fluorescent enzymatic reaction product, NADH. LDH activity was found not to be a unique marker for diagnosis of leukemia. Reactions of single LDH-1 molecules were investigated by monitoring the reaction product with LIF detection.
Download or read book Two wavelength Laser Induced Fluorescence Detection for Capillary Electrophoresis of Proteins and Peptides written by Guido Fridolin Verbeck and published by . This book was released on 1999 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book UV Laser and LED Induced Fluorescence Spectroscopy for Detection of Trace Amounts of Organics in Drinking Water and Water Sources written by Anna V. Sharikova and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: ABSTRACT: A UV Laser Induced Fluorescence (LIF) system, previously developed in our laboratory, was modified and used for a series of applications related to the development and optimization of UV LIF spectroscopic measurements of trace contaminants in drinking water and other water sources. Fluorescence spectra of a number of water samples were studied, including those related to the reverse osmosis water treatment and membrane fouling, domestic and international drinking water, industrial toxins, bacterial spores, as well as several fluorescence standards. Of importance was that the long term detection of the trace level of Dissolved Organic Compounds (DOC) was measured, for the first time to our knowledge, over a one week period and with a time resolution of 2.5 minutes. A comparison of LIF emission using both 266 nm and 355 nm excitation was also made for the first time. Such real-time and continuous measurements are important for future water treatment control. The LIF system was modified to accommodate UV Light Emitting Diodes (LED) as alternative excitation sources, and tested for the detection of trace organic species in water. In addition, a compact system using LED excitation and a spectrometer was xviii developed and underwent initial testing. The original LIF system had two laser sources, 266 nm and 355 nm. The additional sources incorporated in the system were UV LEDs emitting at 265 nm, 300 nm, 335 nm and 355 nm. The LED spectral emission was studied in detail, in terms of spectral variability and power output. It was found that all LEDs had some emission in the visible spectrum, and an optical filter was used to remove it. The signal-to-noise ratio for the LED-based systems was determined and compared with that of the LIF system. The fluorescent signal of the LED-based system was smaller by 1 to 2 orders of magnitude, despite the fact that the LED pulse energy was 2 to 3 orders of magnitude less than the laser's. As such, the fluorescent signal from the LED was greater than expected. Therefore, a UV LED may be a compact and much cheaper optical source for future water measurement instruments.
Download or read book Applications of Capillary Electrophoresis and Laser induced Fluorescence Detection to the Analysis of Trace Species written by Qifeng Xue and published by . This book was released on 1995 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Site specific Fluorescent Derivatization of Peptides for Characterization by Capillary Electrophoresis with Laser induced Fluorescence Detection written by Sara Jane Sudmeier and published by . This book was released on 1998 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1995 with total page 1476 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Capillary Electrophoresis with Laser Induced Fluorescence for DNA Damage Analysis written by Erwin Oliver V. Fundador and published by . This book was released on 2007 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Near infrared Laser Induced Fluorescence as an Ultra Sensitive Method of Detection for Capillary Electrophoresis written by Menno J. Baars and published by . This book was released on 1998 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fluorescence detection techniques in liquid chromatography written by Lars Johnson and published by . This book was released on 1982 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Analysis of Large Biomolecules by Capillary Electrophoresis with Laser induced Fluorescence Detection written by Pertti Juha Viskari and published by . This book was released on 2003 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Diode Laser Induced Fluorescence Detection for the Far red Analysis of Pharmaceutical Compounds by Capillary Electrophoresis and Capillary Electrochromatography written by Sriram Nagaraj and published by . This book was released on 1999 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Preconcentration Techniques in Capillary Electrophoresis with Laser induced Fluorescence Detection written by Jiang Jiang and published by . This book was released on 2005 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt:
