Download or read book Cloning and Characterization of Putative Molecular Targets of Penicillium Marneffei Identified by Random Genome Exploration written by Yee-Lam Elim Cheung and published by . This book was released on 2017-01-26 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Cloning and Characterization of Putative Molecular Targets of Penicillium Marneffei Identified by Random Genome Exploration" by Yee-lam, Elim, Cheung, 張以琳, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled Cloning and characterization of putative molecular targets of Penicillium marneffei identified by random genome exploration Submitted by Cheung Yee Lam Elim for the degree of Master of Philosophy at the University of Hong Kong in December 2002 Penicillium marneffei is a dimorphic fungus causing intracellular infection of the reticulo-endothelial system in humans and bamboo rats. It is endemic in Southeast Asia and causes opportunistic infection in about 10% of AIDS patients in this region. The absence of an identified sexual stage of P. marneffei, the potentially infectious nature of the mould phase conidia, and the difficulty of achieving a complete yeast form of the tissue-invasive phase in vitro have markedly impaired the understanding of the molecular biology and biochemical mechanisms in the thermal dimorphic switching and host-microbe interactions of this fungus. Its thermal dimorphism and the propensity to cause infection in the AIDS patients makes it an ideal candidate for genomic analysis which may provide crucial information in understanding the morphogenesis of the fungus and its interaction with the immune system in pathogenesis. Pulsed field gel electrophoresis of the genomic DNA of P. marneffei showed the presence of three (5.0 Mb, 4.0 Mb, and 2.2 Mb) or more chromosomes. Results obtained from telomeric fingerprinting revealed the presence of 6-12 bands, suggesting that there were chromosomes of similar sizes. The genome size of P. marneffei was hence about 17.8-26.2 Mb. Random exploration of the genome of P. marneffei yielded 2303 random sequence tags (RSTs), corresponding to 10-15% of the genome. The G+C content of the genome is 48.8%. 11.7%, 6.3%, and 17.4% of the RSTs showed homology to yeast-specific sequences, fungus non-yeast sequences, and sequences common to both forms respectively. Analysis of the RSTs revealed genes for information transfer (ribosomal protein genes, tRNA synthetase subunits, translation initiation and elongation factors), metabolism, and compartmentalization, (several multi-drug resistance protein genes and homologues of fluconazole resistance gene). The presence of genes encoding pheromone homologues and ankyrin repeat- containing proteins of other fungi and algae strongly suggests the presence of a sexual stage that presumably exists in environmental conditions. Random exploration of the P. marneffei genome revealed sequences that encode putative methionine aminopeptidase (Met-AP2), acid protease, phospholipase B, and DnaJ. These represent putative virulence factors and molecular targets for antifungal development. Comparison of the cDNA sequences and the genomic DNA sequences revealed that there are six and two introns in MAP2 gene and phospholipase B gene respectively, whereas the acid protease and dnaJ genes contain no intron. None of the four proteins were immunogenic. Characterizations of the functions of Met-AP2 and acid protease were carried out. For Met-AP2, complementation experiments showed that the function of Met-AP in Met-AP deficient E. coli can be complemented by Met-AP2 of P. marneffei. As for the acid protease, results from zymography and protease assay using azocasein as substrate revealed that the purified acid protease functioned best at pH 5. DOI: 10.5353/th_b2664260 Subjects: Penicillium -

Download or read book Clinical Mycology written by William E. Dismukes and published by . This book was released on 2003 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: Within the field of infectious diseases, medical mycology has experienced significant growth over the last decade. Invasive fungal infections have been increasing in many patient populations, including: those with AIDS; transplant recipients; and the elderly. As these populations grow, so does the diversity of fungal pathogens. Paralleling this development, there have been recent launches of several new antifungal drugs and therapies. Clinical Mycology offers a comprehensive review of this discipline. Organized by types of fungi, this volume covers microbiologic, epidemiologic and demographic aspects of fungal infections as well as diagnostic, clinical, therapeutic, and preventive approaches. Special patient populations are also detailed.

Download or read book Fungal Pigments written by Laurent Dufossé and published by MDPI. This book was released on 2018-03-23 with total page 141 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "Fungal Pigments" that was published in JoF

Download or read book Pathogen Genomics written by Karen Joy Shaw and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 513 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading infectious disease researchers and pharmaceutical scientists comprehensively review the latest genomic technologies and their application to the prevention, diagnosis, and treatment. The authors' cross-disciplinary approach, with expertise acquired from studying disease-causing viruses, bacteria, fungi, and protozoa, reveals how sequence information from diverse pathogens has uncovered novel targets for drug discovery, antigenic determinants for vaccine development, as well as diagnostic tools. The authors also discuss the application of DNA micoarrays and the impact of genome sequencing comparisons on the discovery and choices of novel drug targets. The result is a better understanding how genomic information can reveal the fundamentals of microbial pathogenesis and how organisms interact with their host environment.

Download or read book Fungal Wilt Diseases of Plants written by Marshal Mace and published by Elsevier. This book was released on 2012-12-02 with total page 655 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fungal Wilt Diseases of Plants focuses on wilt diseases caused by the fungal genera Verticillium, Fusarium, and Ceratocystis. Special attention is given to the interactions of physiological, biochemical, and anatomical factors, as these relate to pathogenesis and mechanisms of disease resistance. Organized into 16 chapters, this book begins with a description, in a historical perspective, of the major research themes in fungal wilt diseases. It then looks into the worldwide status of this plant disease. The three subsequent chapters describe the epidemiology and life cycle of the major fungal wilt pathogens in Fusarium, Verticillium, and Ceratocystis. This book also provides an in-depth view of the genetics and biochemistry of these pathogens; the nature of pathogenesis and the effects of wilt pathogens on host-water relations; and the sources and genetics of host resistance in field and fruit crops, vegetable crops, and shade trees. Other chapters are dedicated to the biochemistry, physiology, and the anatomical aspects of resistance and to the progress in the biological and chemical control of these pathogens. This text will be of great value to graduate students and senior research scientists in plant pathology, physiology, and biochemistry, who are specifically involved in studying wilt diseases and host-parasite interactions. It will provide them the detailed background information needed to supplement their specialized research interests.

Download or read book Yeast Stress Responses written by Stefan Hohmann and published by Springer Science & Business Media. This book was released on 2007-10-23 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: Every cell has developed mechanisms to respond to changes in its environment and to adapt its growth and metabolism to unfavorable conditions. The unicellular eukaryote yeast has long proven as a particularly useful model system for the analysis of cellular stress responses, and the completion of the yeast genome sequence has only added to its power This volume comprehensively reviews both the basic features of the yeast genral stress response and the specific adapations to different stress types (nutrient depletion, osmotic and heat shock as well as salt and oxidative stress). It includes the latest findings in the field and discusses the implications for the analysis of stress response mechanisms in higher eukaryotes as well.

Download or read book Molecular Identification of Fungi written by Youssuf Gherbawy and published by Springer Science & Business Media. This book was released on 2010-03-03 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fungi enjoy great popularity in pharmaceutical, agricultural, and biotechnological applications. Recent advances in the decipherment of whole fungal genomes promise an acceleration of these trends. This timely book links scientists from different parts of the world who are interested in the molecular identification of fungi combined with the exploration of the fungal biodiversity in different ecosystems. It provides a compendium for scientists who rely on a rapid and reliable detection of fungal specimens in environmental as well as clinical resources in order to ensure the benefit of industrial and clinical applications. Chapters focus on the opportunities and limits of the molecular marker-mediated identification of fungi. Various methods, procedures and strategies are outlined. Furthermore, the book offers an update of the current progress in the development of fungal molecular techniques, and draws attention to potential and associated problems, as well as integrating theory and practice.

Download or read book The Rasputin Effect When Commensals and Symbionts Become Parasitic written by Christon J. Hurst and published by Springer. This book was released on 2016-07-05 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on those instances when benign and even beneficial relationships between microbes and their hosts opportunistically change and become detrimental toward the host. It examines the triggering events which can factor into these changes, such as reduction in the host’s capacity for mounting an effective defensive response due to nutritional deprivation, coinfections and seemingly subtle environmental influences like the amounts of sunlight, temperature, and either water or air quality. The effects of environmental changes can be compounded when they necessitate a physical relocation of species, in turn changing the probability of encounter between microbe and host. The change also can result when pathogens, including virus species, either have modified the opportunist or attacked the host’s protective natural microflora. The authors discuss these opportunistic interactions and assess their outcomes in both aquatic as well as terrestrial ecosystems, highlighting the impact on plant, invertebrate and vertebrate hosts.

Download or read book Guide to Yeast Genetics and Molecular Biology written by Christine Guthrie and published by . This book was released on 1991-01-28 with total page 933 pages. Available in PDF, EPUB and Kindle. Book excerpt: Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Basic Methods in Yeast Genetics**Physical and genetic mapping**Making and recovering mutants**Cloning and Recombinant DNA Methods**High-efficiency transformation**Preparation of yeast artificial chromosome vectors**Basic Methods of Cell Biology**Immunomicroscopy**Protein targeting assays**Biochemistry of Gene Expression**Vectors for regulated expression**Isolation of labeled and unlabeled DNA, RNA, and protein

Download or read book Progress in Mycology written by M.K. Rai and published by Scientific Publishers. This book was released on 2017-02-01 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present book is aimed to provide the readers with current trends in the field of Mycology in general and fungal biotechnology in particular. The book would be of utmost importance to students, researchers and teachers of botany, mycology, microbiology, fungal biotechnology and nanotechnology. The readers should find the book full of information and reader-friendly.

Download or read book Plant Relationships written by Holger B. Deising and published by Springer Science & Business Media. This book was released on 2009-02-07 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the first edition of "The Mycota Vol. V – Plant Relationships" in 1997, tremendous advances in fungal molecular biology and biochemistry have taken place; and both light and electron microscopical techniques have improved considerably. These new insights led to a better understanding of the relationships between fungi and plants; and a completely revised new edition of Plant Relationships could be produced, providing an up-to-date overview on mutualistic and pathogenic interactions. In 18 chapters internationally acknowledged authors present reviews on fungal lifestyles, mechanisms of their interactions with their host plants, signal perception and transduction, and plant defense responses directed against attack by fungal pathogens. Highlighting the recent developments in fungus-plant interactions, this volume is indispensable for researchers, lecturers and students in microbiology, mycology and plant sciences, including plant pathology.

Download or read book Recent Developments on Genus Chaetomium written by Ahmed M. Abdel-Azeem and published by Springer Nature. This book was released on 2019-12-27 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chaetomium genus was established by Gustav Kunze in 1817. According to Index Fungorum Partnership, there are 273 Chaetomium species accepted till now. Members of the genus Chaetomium are capable of colonizing various substrates and are well-known for their ability to degrade cellulose and to produce a variety of bioactive metabolites. More than 200 compounds have been reported from this genus. A huge number of new and bioactive secondary metabolites associated with unique and diverse structural types, such as chaetoglobosins, epipolythiodioxopiperazines, azaphilones, depsidones, xanthones, anthraquinones, chromones, and steroids, have been isolated and identified. Many of the compounds have been reported to possess significant biological activities, such as antitumor, antimalarial, cytotoxic, enzyme inhibitory, antimicrobial, phytotoxic, antirheumatoid and other activities. Chaetomium taxa are frequently reported to be cellulase and ligninase producers with the ability to degrade cellulosic and woody materials. This is the first, comprehensive volume covering Chaetomium genus in detail. It includes the latest research, methods, and applications, and was written by scholars working directly in the field. The book also contains informative illustrations and is fully referenced for further reading.

Download or read book Microbial Genomics in Sustainable Agroecosystems written by Vijay Tripathi and published by Springer Nature. This book was released on 2019-11-23 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: Today, microbiology is a rapidly growing discipline in the life sciences, and the technologies are evolving on a virtually daily basis. Next-generation sequencing technologies have revolutionized microbial analysis, and can help us understand the biology and genomic diversity of various bacterial species with significant impacts on agro-ecosystems. In addition, advances in molecular biology and microbiology techniques hold the potential to improve the productivity and sustainability of agriculture and forestry. This new volume addresses the role of microbial genomics in understanding the living systems that exist in the soil and their interactions with plants, an aspect that is also important for crop improvement. The topics covered focus on a deeper and clearer understanding of how microbes cause diseases, the genome-based development of novel antibacterial agents and vaccines, and the role of microbial genomics in crop improvement and agroforestry. Given its scope, the book offers a valuable resource for researchers and students of agriculture and infectious biology.

Download or read book Natural Bioactive Products in Sustainable Agriculture written by Joginder Singh and published by Springer Nature. This book was released on 2020-05-11 with total page 307 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses various aspects of bioactive natural products employed in the agrochemical and agriculture sectors. It covers the use of plants, microorganisms, and microbial metabolites as eco-friendly, cost-effective, and sustainable alternatives to chemicals in the field of agriculture. Written by active researchers and academics, the book highlights state-of-art products in the field, as well as the gaps, challenges, and obstacles associated with the use of plants, microbes and their products. Given its scope, it is a valuable resource for the scientific community and professionals in enterprises wanting insights into the latest developments and advances in the context of biological products, including their applications, traditional uses, modern practices, and strategies to harness their full potential.

Download or read book Biotechnology of Anti diabetic Medicinal Plants written by Saikat Gantait and published by Springer Nature. This book was released on 2021-11-13 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a unique overview of insights on the genetic basis of anti-diabetic activity, chemistry, physiology, biotechnology, mode-of-action, as well as cellular mechanisms of anti-diabetic secondary metabolites from medicinal plants. The World Health Organization estimated that 80% of the populations of developing countries rely on traditional medicines, mostly plant drugs, for their primary health care needs. There is an increasing demand for medicinal plants having anti-diabetic potential in both developing and developed countries. The expanding trade in medicinal plants has serious implications on the survival of several plant species, with many under threat to become extinct. This book describes various approaches to conserve these genetic resources. It discusses the whole spectrum of biotechnological tools from micro-propagation for large-scale multiplication, cell-culture techniques to the biosynthesis and enhancement of pharmaceutical compounds in the plants. It also discusses the genetic transformation as well as short- to long-term conservation of plant genetic resources via synthetic seed production and cryopreservation, respectively. The book is enriched with expert contributions from across the globe. This reference book is useful for researchers in the pharmaceutical and biotechnological industries, medicinal chemists, biochemists, botanists, molecular biologists, academicians, students as well as diabetic patients, traditional medicine practitioners, scientists in medicinal and aromatic plants, Ayurveda, Siddha, Unani and other traditional medical practitioners.

Download or read book Seagrasses of Australia written by Anthony W. D. Larkum and published by Springer. This book was released on 2018-07-27 with total page 791 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book takes the place of “Biology of Seagrasses: A Treatise on the Biology of Seagrasses with Special Reference to the Australian Region”, co-edited by A.W.D. Larkum, A.J. MaCComb and S.A. Shepherd and published by Elsevier in 1989. The first book has been influential, but it is now 25 years since it was published and seagrass studies have progressed and developed considerably since then. The design of the current book follows in the steps of the first book. There are chapters on taxonomy, floral biology, biogeography and regional studies. The regional studies emphasize the importance of Australia having over half of the world’s 62 species, including some ten species published for Australia since the previous book. There are a number of chapters on ecology and biogeography; fish biology and fisheries and dugong biology are prominent chapters. Physiological aspects again play an important part, including new knowledge on the role of hydrogen sulphide in sediments and on photosynthetic processes. Climate change, pollution and environmental degradation this time gain an even more important part of the book. Decline of seagrasses around Australia are also discussed in detail in several chapters. Since the first book was published two new areas have received special attention: blue carbon and genomic studies. Seagrasses are now known to be a very important player in the formation of blue carbon, i.e. carbon that has a long turnover time in soils and sediments. Alongside salt marshes and mangroves, seagrasses are now recognized as playing a very important role in the formation of blue carbon. And because Australia has such an abundance and variety of seagrasses, their role in blue carbon production and turnover is of great importance. The first whole genomes of seagrasses are now available and Australia has played an important role here. It appears that seagrasses have several different suites of genes as compared with other (land) plants and even in comparison with freshwater hydrophytes. This difference is leading to important molecular biological studies where the new knowledge will be important to the understanding and conservation of seagrass ecosystems in Australia. Thus by reason of its natural abundance of diverse seagrasses and a sophisticated seagrass research community in Australia it is possible to produce a book which will be attractive to marine biologists, coastal scientists and conservationists from many countries around the world.

Download or read book Bio pigmentation and Biotechnological Implementations written by Om V. Singh and published by John Wiley & Sons. This book was released on 2017-08-07 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent technological advances have provided unique opportunities for the exploration of alternatives to the industrial use of chemically produced synthetic colors. The most promising developments in this area have been in bio-pigmentation derived from microorganisms. This groundbreaking book reviews the current state of the science of bio-pigmentation, providing important insights into the molecular mechanisms of microbial biosynthesis of industrial pigments. Featuring contributions by leading researchers from both industry and academe, it explores the latest advances in the use of bio-pigments as safe, sustainable alternatives to chemically synthesized pigments, and provides extensive coverage the most promising sources of bio-pigments within the food, feed, and pharmaceutical industries. • Proposes microbial uniqueness of coloration in variety of food, feed and pharmaceuticals • Covers the basic science behind bio-pigmentation as well as the latest advances in the field • Describes detection strategies for screening and identifying color producing microorganisms under varying environmental conditions • Provides an exhaustive review of the literature on color producing extremophiles and offers fascinating insights into color production as a stress response in extremophiles • Explores microbial molecular mechanisms of color production, with special coverage of color production as secondary metabolites under environmental stress Bio-pigmentation and Biotechnological Implementations is required reading for professionals and post-doctoral students of microbiology, applied microbiology, food microbiology, food science, and food biochemistry. It is a valuable working resource for scientists working in color-dependent food, feed, and pharmaceuticals.