Download or read book Characterization of the Large T Antigen Tau Antigen Complex in Simian Virus 40 transformed Rat Cells written by Martin I. Freed and published by . This book was released on 1982 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Purification and Characterization of Simian Virus 40 Large T Antigen written by Raymond James Paxton and published by . This book was released on 1983 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of the Complex Between SV40 Large T Antigen and the 53K Host Protein in Transformed Mouse Cells written by and published by . This book was released on 1980 with total page 9 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Isolation and Characterization of Discrete Subclasses of SV40 Large T Antigen from Lytically Infected and Transformed Monkey Cells written by Lori Covey-Nichols and published by . This book was released on 1986 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book GENETIC AND BIOCHEMICAL ANALYSIS OF SV40 LARGE T ANTIGEN HOST INTERACTIONS T ANTIGEN written by DAVID CHARLES KOHRMAN and published by . This book was released on 1991 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: p185 may be functionally important.

Download or read book Purification and Characterization of Simian Virus 40 Large T Antigen written by Donald Allen Giacherio and published by . This book was released on 1980 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Simian Virus 40 Large T antigen Deletion Mutants written by Earl T. Sawai and published by . This book was released on 1990 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Structural Distortion Activity of Simian Virus 40 Large T Antigen written by Lei Chen and published by . This book was released on 1998 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book CHARACTERIZATION OF THE T ANTIGEN CONTENT OF SIMIAN VIRUS 40 VIRAL TRANSCRIPTION COMPLEXES written by KENNETH GEORGE HADLOCK and published by . This book was released on 1989 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: of T-antigen with transcription complexes as opposed to, or in addition to, T-antigen activating late transcription in an indirect manner.

Download or read book SV40 T Antigen in Multistep Transformation in Vitro of Human Uroepithelial Cells written by Chinghai Kao and published by . This book was released on 1992 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book A mutational analysis of the SV40 small T antigen written by Pratima Jog and published by . This book was released on 1990 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Mapping and Characterization of the Single stranded DNA Binding Domain of Simian Virus 40 Large T Antigen written by Chunxiao Wu and published by . This book was released on 2001 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Cumulated Index Medicus written by and published by . This book was released on 1994 with total page 1364 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Research Awards Index written by and published by . This book was released on 1982 with total page 1170 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Purification and Partial Characterization of a Mutant Within the Simian Virus 40 Large T antigen DNA Binding Domain written by Clyde C. Jones and published by . This book was released on 1996 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Mutational Analysis of the Central Channel in the Simian Virus 40 Large T Antigen Helicase written by David Manna and published by . This book was released on 2006 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: I report in this dissertation that residues lining the small tier of the central channel have an important role in DNA binding, especially in single-stranded DNA binding. Defects in helicase activity for the small tier channel mutants can be accounted by their corresponding defects in DNA binding. One of the large tier central channel mutants (R456A) showed a defect in unwinding of origin DNA, but this could not be attributed to a problem in DNA binding. Combined with the results of others, it appears that the large tier is primarily involved in unwinding origin DNA. These findings are incorporated into a model of T antigen helicase activity where single-stranded DNA is looped out of one hexamer through hydrophilic side-channels in the large tier of the hexamer. The looped-out single-stranded DNA runs back into the origin binding domain of the adjacent hexamer. In this model, there is one strand of DNA within each small tier during DNA unwinding from the origin. (Abstract shortened by UMI.).

Download or read book Mutational Analysis of the Origin Binding Domain of Simian Virus 40 Large T antigen Provides Insights Into Its Function in DNA Unwinding written by Erin C. Foster and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA replication is an essential process of cell division required in order to maintain genomic integrity of all dividing cells. Although advancements have been made toward further understanding of this process, much is still unknown about the mechanisms underlying DNA replication, especially in mammalian systems. Eukaryotic DNA replication is a tightly regulated and complex process involving a myriad of proteins and numerous origins. Due to this complexity Simian Virus 40 (SV40) is used as a model system to simplify the investigation of the function of individual protein components. The replication of SV40 DNA employs one multifunctional viral protein, large T-antigen (T-ag). Many structural and genetic studies have provided a wealth of information about T-ag; however much remains unknown about the specific interactions between T-ag, DNA and replication factors and the precise sequence of events during replication. SV40 large T-ag is a multidomain protein that functions in origin recognition and unwinding events during initiation of SV40 DNA replication. The Origin Binding Domain (OBD) of T-ag plays a critical role during initiation to position T-ag on the origin through the use of a DNA binding motif consisting of A1 and B2 loops. After origin recognition, T-ag forms a double hexamer over the origin. Within each hexamer, the OBD adopts a lock washer structure where the origin recognizing A1 and B2 loops face towards the helicase domain and likely become unavailable for DNA binding. New surfaces of the OBD monomers are positioned along the DNA axis during hexamer formation. The role of the central channel of the OBD hexamer in DNA replication was investigated by analyzing the effects of mutations of residues lining the channel. All mutants showed binding defects with origin DNA and ssDNA especially at low protein concentrations, but only half were defective at supporting DNA replication in vitro. All mutants were normal in unwinding linear origin DNA fragments. However, replication defective mutants failed to unwind a small origin containing circular DNA whereas replication competent mutants did so normally. The presence of RPA and/or pol/prim restored circular DNA unwinding activity of compromised mutants probably by interacting with the separated DNA strands on the T-ag surface. The addition of RPA also recovered the ability of replication deficient mutants to support initiation and complete DNA replication in vitro. The addition of pol/prim resulted in the greatest recovery of mutant activity in minicircle unwinding and initiation. Compared to other polymerases tested, pol/prim was the only polymerase able to rescue mutant activity to WT levels indicated that this stimulation is specific to pol/prim. Of the four subunits of the pol/prim complex that could be responsible for stimulating mutant activity, the p180 subunit appeared to be active in recovering circular DNA unwinding. These results indicated a role for the OBD central channel in binding and threading ssDNA during unwinding of circular SV40 DNA. Mixing mutant and WT T-ag in minicircle unwinding assays suggested that only one monomer in an OBD hexamer was necessary for DNA unwinding. Results allowed for the development of a model of DNA threading through the T-ag complex illustrating how single stranded DNA could pass close to a trough generated by key residues in one monomer of the OBD hexamer.