Download or read book Characterization of the BglG Protein of Escherichia Coli K 12 and Its RNA Binding Activity written by Fariba Houman and published by . This book was released on 1990 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Transcriptional Regulation of the Escherichia Coli K 12 Grx Gene written by Weiyin Shen and published by . This book was released on 1997 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook on Clostridia written by Peter Duerre and published by CRC Press. This book was released on 2005-03-29 with total page 919 pages. Available in PDF, EPUB and Kindle. Book excerpt: Clostridia is one of the largest bacterial genera with an enormous potential for biotechnical and medical applications. Despite growing scientific, medical, and industrial interest, information on basic methods, biochemical fundamentals, clinical practice, industrial applications, and novel developments remains scattered in a variety of research ar
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1996 with total page 1340 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemical Abstracts written by and published by . This book was released on 2002 with total page 2626 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 1938 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Download or read book Systematic Mutagenesis of Escherichia Coli Genome and Genome wide Expression Analysis Indicates that FNR of E Coli K12 Regulates a Large Number of Genes of Unknown Function written by Yisheng Kang and published by . This book was released on 2005 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bacterial Signaling written by Reinhard Krämer and published by John Wiley & Sons. This book was released on 2009-12-09 with total page 513 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing a comprehensive insight into cellular signaling processes in bacteria with a special focus on biotechnological implications, this is the first book to cover intercellular as well as intracellular signaling and its relevance for biofilm formation, host pathogen interactions, symbiotic relationships, and photo- and chemotaxis. In addition, it deals in detail with principal bacterial signaling mechanisms -- making this a valuable resource for all advanced students in microbiology. Dr. Krämer is a world-renowned expert in intracellular signaling and its implications for biotechnology processes, while Dr. Jung is an expert on intercellular signaling and its relevance for biomedicine and agriculture.
Download or read book Characterization of Era an Essential GTP binding Protein in Escherichia Coli written by Poonam Sood and published by . This book was released on 1993 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Gene Regulation in Escherichia Coli Beyond the 0 rate0 Approximation written by Lok-hang So and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The blueprint of a living cell is inscribed in its DNA. A region of DNA encoding a protein is called a gene. The cell reads the DNA and makes molecular machines made up of proteins to carry out all cellular functions required for survival. All cells live in ever-changing environments, and have different needs at different times. The control of when and how often each protein is produced from a gene is called gene regulation. Transcription, the copying of a DNA sequence into a complementary mRNA molecule, is the first step in the information flow from DNA to proteins, and most regulation is already done at the transcription level to avoid the production of superfluous intermediates. A living cell takes environmental stimuli as input, and regulates the activity of genes through DNA-binding proteins called transcription factors. The activity of a gene is described by its time-series of discrete mRNA production events. The events constituting this transcriptional time-series are stochastic and exhibit intermittent, bursty behavior, in bacteria as well as higher organisms. Thus the transcriptional time-series cannot be fully described by a simple chemical 0́−rate0́+0́4the probability per unit time of transcribing an mRNA molecule. An important consequence of this temporal complexity is that gene expression level can be tuned by varying different features of the time-series. It is then natural to ask: What modulation scheme is used by the cell to change expression levels of genes? Furthermore, if we look at the transcriptional time-series of multiple genes, would we see different modulation schemes for different genes, or a common modulation scheme shared by all genes? Last but not least, what is the molecular mechanism leading to bursty transcriptional time-series? What are the biophysical states that correspond to the active and inactive periods in a bursty transcriptional time-series? To answer these questions, I characterized the mRNA copy-number statistics from multiple promoters in the model organism Escherichia coli under various growth conditions using single-molecule fluorescence in situ hybridization. The kinetics of the underlying transcriptional time-series was then inferred using the two-state model, a simple stochastic mathematical model that describes bursty transcription time-series. I found that the degree of burstiness depends only on the gene expression level, while being independent of the details of gene regulation. The observed behavior is explained by the underlying variation in the duration of bursting events. At this stage, there is no mechanistic, molecular-level understanding of what gives rise to the bursty behavior of gene activity in bacteria. However, my finding here, that the properties of the transcriptional time-series are gene-independent rather than gene-specific, is contrary to the most common theoretical model used to explain bursty transcriptional time-series in bacteria, which involves the binding and unbinding of transcription factors at the promoter. My data suggests that the observed bursty kinetics arises from gene-nonspecific mechanisms such as DNA topology modulation, RNA polymerase dynamics, or regulation by broad-target DNA-binding proteins. Further investigation would narrow down the source of bursty transcriptional time-series.
Download or read book Biochemical and Genetic Characterization of the E Coli RadD and RecG Proteins written by Nina Bonde and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Faithful replication of the genomic material is a requirement for successful cell propagation and division in all organisms. In Escherichia coli, the RadD and RecG proteins play important roles in DNA repair and recombination. This thesis reveals an important molecular function for RadD and examines RecG protein and gene interactions that help modulate and define its function in cells. We describe a new molecular function for RadD. RadD accelerates RecA-mediated strand exchange in vitro. The ability of RadD to accelerate strand exchange is dependent on both its ATP hydrolysis activity and the presence of RecA. RadD is unable to remodel recombination intermediate molecules in the absence of RecA. The dependence on RecA to carry out this function places RadD in a new class of enzymes. RecG physically interacts with the single-stranded DNA-binding protein (SSB). We report that this interaction is mediated by the highly conserved SSB C-terminal tip (SSB-Ct). The SSB-Ct docks onto a conserved region of the RecG Helicase Domain 2, and the SSB-Ct binding pocket on RecG is framed by three Arg residues. Charge-reversal mutations to any of the three Arg residues results in loss of the RecG-SSB interaction and cellular defects, including induced SOS response and sensitivity to ultraviolet radiation. As a complement to this study, we also report that the intrinsically disorder linker of SSB is not necessary under normal growth conditions and likely acts as a simple spacer between the SSB OB domain and SSB-Ct interaction motif. RecG function has been difficult to elucidate in vivo. We identify recG genetic interactions by transposon sequencing to gain insight into the activity of recG in cells. The strongest genetic interactions occur with the dam, uvrD, rnhA, radA, and rep genes. Viability of strains lacking recG in addition to one of these five genes is generally improved by additional loss of the recF or recO genes. recG mutants that are deficient in SSB binding produce similar phenotypes as cells lacking recG. Collectively, these results support a role for RecG in alleviating toxic recombination intermediates.
Download or read book The Purification Genetical and Biophysical Characterization of the CysJ Gene Protein from Escherichia Coli K 12 written by John Alexander Loudon and published by . This book was released on 1982 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Characterization of the OmpG Porin Protein of Escherichia Coli K 12 written by Daniel Adrian Fajardo and published by . This book was released on 1997 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization of a novel mutation in the o m p C gene of Escherichia Coli K 12 which affects expression of the major outer membrane proteins written by Katrina Mary Catron and published by . This book was released on 1987 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization of the GlpD and GlpEGR Operons of Escherichia Coli K 12 written by T. Denise Austin and published by . This book was released on 1991 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization of Escherichia Coli K 12 Mutants with Increased Expression of an Osmotically Regulated Gene written by Elizabeth Anne DiBlasio and published by . This book was released on 1986 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biophysical and RNA binding Characterization of Hfq Proteins from Neisseria Meningitidis Vibrio Cholerae and Escherichia Coli written by Tomasz Krzysztof Kabziński and published by . This book was released on 2012 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt:
