Download or read book Characterization of Metal reducing Microbial Communities from Acidic Subsurface Sediments Contaminated with Uranium VI written by Ellen McLain Edwards and published by . This book was released on 2005 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt: Iron(III)-reducing bacteria (FeRB) have been demonstrated to rapidly catalyze U(VI) reduction and Fe(III) is an abundant electron acceptor in uranium-contaminated subsurface sediments. Thus, FeRB communities were the focus of this dissertation. The abundance, diversity, and activity of indigenous metal-reducing microbial communities likely to contribute to uranium reduction was examined in the field and under more controlled conditions in the laboratory.
Download or read book Analysis of Microbial Communities in a Contaminated Aquifer Undergoing Uranium Bioremediation written by Erick Cardenas Poire and published by . This book was released on 2009 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Characterization of a Microbial Community from Uranium contaminated Acidic Sediment written by Nadia North and published by . This book was released on 2003 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microbial Metal Respiration written by Johannes Gescher and published by Springer Science & Business Media. This book was released on 2014-02-21 with total page 237 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbes can respire on metals. This seemingly simple finding is one of the major discoveries that were made in the field of microbiology in the last few decades. The importance of this observation is evident. Metals are highly abundant on our planet. Iron is even the most abundant element on Earth and the forth most abundant element in the Earth’s crust. Hence, in some environments iron, but also other metals or metalloids, are the dominant respiratory electron acceptors. Their reduction massively drives the carbon cycle in these environments and establishes redox cycles of the metallic electron acceptors themselves. These redox cycles are not only a driving force for other biotic reactions but are furthermore necessary for initiating a number of geochemically relevant abiotic redox conversions. Although widespread and ecologically influential, electron transfer onto metals like ferric iron or manganese is biochemically challenging. The challenge is to transfer respiratory electrons onto metals that occur in nature at neutral pH in the form of metal oxides or oxihydroxides that are effectively insoluble. Obviously, it is necessary that the microbes specially adapt in order to catalyze the electron transfer onto insoluble electron acceptors. The elucidation of these adaptations is an exciting ongoing process. To sum it up, dissimilatory metal reduction has wide-spread implications in the field of microbiology, biochemistry and geochemistry and its discovery was one of the major reasons to establish a novel scientific field called geomicrobiology. Recently, the discovery of potential applications of dissimilatory metal reducers in bioremediation or current production in a microbial fuel cell further increased the interest in studying microbial metal reduction.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2007 with total page 960 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Uranium Mining and Hydrogeology written by Broder J. Merkel and published by Springer Science & Business Media. This book was released on 2008-09-30 with total page 977 pages. Available in PDF, EPUB and Kindle. Book excerpt: Subject of the book is Uranium and its migration in aquatic environments. The following subjects are emphasised: Uranium mining, Phosphate mining, mine closure and remediation, Uranium in groundwater and in bedrock, biogeochemistry of Uranium, environmental behavior, and modeling. Particular results from the leading edge of international research are presented.
Download or read book Microbial Metal and Metalloid Metabolism written by John F. Stolz and published by American Society for Microbiology Press. This book was released on 2011-08-02 with total page 562 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ideal reference for novice and experienced investigators interested in environmental biogeochemistry and bioremediation. • Offers a broad range of current topics and approaches in microbe-metal research, including microbial fuel cells, unique microbial physiology, genomics, proteomics, and transcriptomics. • Reviews the current state of the science in the field, and examines emerging developments and applications and forecasts future research directions. • The book is also recommended as a text for graduate courses in microbial physiology, microbial ecology, and applied and environmental microbiology.
Download or read book Recent Advances in Bioremediation biodegradation by Extreme Microorganisms 2nd Edition written by Edgardo Donati and published by Frontiers Media SA. This book was released on 2021-11-19 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt: Publisher’s note: This is a 2nd edition due to an article retraction.
Download or read book Idet written by and published by . This book was released on 1950 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book STRUCTURE AND FUNCTION OF SUBSURFACE MICROBIAL COMMUNITIES AFFECTING RADIONUCLIDE TRANSPORT AND BIOIMMOBILIZATION written by and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The objectives of this project were to: (1) isolate and characterize novel anaerobic prokaryotes from subsurface environments exposed to high levels of mixed contaminants (U(VI), nitrate, sulfate), (2) elucidate the diversity and distribution of metabolically active metal- and nitrate-reducing prokaryotes in subsurface sediments, and (3) determine the biotic and abiotic mechanisms linking electron transport processes (nitrate, Fe(III), and sulfate reduction) to radionuclide reduction and immobilization. Mechanisms of electron transport and U(VI) transformation were examined under near in situ conditions in sediment microcosms and in field investigations at the Oak Ridge Field Research Center (ORFRC), in Oak Ridge, Tennessee, where the subsurface is exposed to mixed contamination predominated by uranium and nitrate. A total of 20 publications (16 published or 'in press' and 4 in review), 10 invited talks, and 43 contributed seminars/ meeting presentations were completed during the past four years of the project. PI Kostka served on one proposal review panel each year for the U.S. DOE Office of Science during the four year project period. The PI leveraged funds from the state of Florida to purchase new instrumentation that aided the project. Support was also leveraged by the PI from the Joint Genome Institute in the form of two successful proposals for genome sequencing. Draft genomes are now available for two novel species isolated during our studies and 5 more genomes are in the pipeline. We effectively addressed each of the three project objectives and research highlights are provided. Task I - Isolation and characterization of novel anaerobes: (1) A wide range of pure cultures of metal-reducing bacteria, sulfate-reducing bacteria, and denitrifying bacteria (32 strains) were isolated from subsurface sediments of the Oak Ridge Field Research Center (ORFRC), where the subsurface is exposed to mixed contamination of uranium and nitrate. These isolates which are new to science all show high sequence identity to sequences retrieved from ORFRC subsurface. (2) Based on physiological and phylogenetic characterization, two new species of subsurface bacteria were described: the metal-reducer Geobacter daltonii, and the denitrifier Rhodanobacter denitrificans. (3) Strains isolated from the ORFRC show that Rhodanobacter species are well adapted to the contaminated subsurface. Strains 2APBS1 and 116-2 grow at high salt (3% NaCl), low pH (3.5) and tolerate high concentrations of nitrate (400mM) and nitrite (100mM). Strain 2APBS1 was demonstrated to grow at in situ acidic pHs down to 2.5. (4) R. denitrificans strain 2APBS1 is the first described Rhodanobacter species shown to denitrify. Nitrate is almost entirely converted to N2O, which may account for the large accumulation of N2O in the ORFRC subsurface. (5) G. daltonii, isolated from uranium- and hydrocarbon-contaminated subsurface sediments of the ORFRC, is the first organism from the subsurface clade of the genus Geobacter that is capable of growth on aromatic hydrocarbons. (6) High quality draft genome sequences and a complete eco-physiological description are completed for R. denitrificans strain 2APBS1 and G. daltonii strain FRC-32. (7) Given their demonstrated relevance to DOE remediation efforts and the availability of detailed genotypic/phenotypic characterization, Rhodanobacter denitrificans strain 2APBS1 and Geobacter daltonii strain FRC-32 represent ideal model organisms to provide a predictive understanding of subsurface microbial activity through metabolic modeling. Tasks II and III-Diversity and distribution of active anaerobes and Mechanisms linking electron transport and the fate of radionuclides: (1) Our study showed that members of genus Rhodanobacter and Geobacter are abundant and active in the uranium and nitrate contaminated subsurface. In the contaminant source zone of the Oak Ridge site, Rhodanobacter spp. are the predominant, active organisms detected (comprising 50% to 100% of rRNA detected). (2) We demonstrated for the first time that the function of microbial communities can be quantified in subsurface sediments using messenger RNA assays (molecular proxies) under in situ conditions. (3) Active Geobacteraceae were identified and phylogenetically characterized from the cDNA of messenger RNA extracted from ORFRC subsurface sediment cores. Multiple clone sequences were retrieved from G. uraniireducens, G. daltonii, and G. metallireducens. (4) Results show that Geobacter strain FRC-32 is capable of growth on benzoate, toluene and benzene as the electron donor, thereby providing evidence that this strain is physiologically distinct from other described members of the subsurface Geobacter clade. (5) Fe(III)-reducing bacteria transform structural Fe in clay minerals from their layer edges rather than from their basal surfaces.
Download or read book Assessment of Bacterial Communities and an Iron reducing Bacterium in Relation to an Engineered Bioremediation System Designed for the Treatment of Uranium nitric Acid Contaminated Groundwater written by Chiachi Hwang and published by . This book was released on 2009 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: The elucidation of how populations of interest interact in a given community and how the community responds to stress and perturbations can provide insight into the interplay between stress pathways and gene networks that help optimize bacterial biochemistry. The goal of the present study was to characterize the responses of bacterial communities at multiple levels of resolution to understand microbial biochemical capacity at DOE waste sites. The field work at the Field Research Center of the U.S. Department of Energy, Oak Ridge, TN, used a field scale denitrifying fluidized bed reactor (FBR) for nitrate treatment of the groundwater and a series of wells to stimulate microbial growth via ethanol for in situ U(VI) immobilization. Bacterial community dynamics were investigated during the initial start-up of the FBR while those from the groundwater of the wells were studied over a 1.5-yr period. In addition, the physiology and the genome of the isolate, Anaeromyxobacter Fw109-5, from the site were studied to examine its potential role in U(VI) remediation. The subsurface environment was altered via engineering controls during successive phases to better understand strategies that would improve the remediation process. Within this framework, the interrelationship of bacterial communities and geochemistry was studied at different spatial and temporal scales to characterize the ecosystem ecology of an engineered system. Bacterial communities from both FBR and groundwater samples were analyzed via clone libraries of partial SSU rRNA genes. Multivariate analyses were applied to correlate the changes in the bacterial communities to the measured physicochemical parameters. Our results from the field experiments indicated that there was an important interaction between the engineering controls that altered the subsurface geochemistry over time that influenced bacterial population responses. Growth study experiments and genomic analysis also revealed insights to the physiological potential of an iron-reducing isolate, Anaeromyxobacter Fw109-5. The strong associations between particular environmental variables and certain population distributions will provide insights into establishing practical and successful remediation strategies in radionuclide-contaminated environments with respect to engineering controls.
Download or read book Characterization of Subsurface Microbial Communities Involved in Bioremediation of Uranium and Nitrate written by Anne Marie Spain and published by . This book was released on 2009 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Environmental Microbiology written by Ralph Mitchell and published by John Wiley & Sons. This book was released on 2010-01-08 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: The bestselling reference on environmental microbiology—now in a new edition This is the long-awaited and much-anticipated revision of the bestselling text and reference. Based on the latest information and investigative techniques from molecular biology and genetics, this Second Edition offers an in-depth examination of the role of microbiological processes related to environmental deterioration with an emphasis on the detection and control of environmental contaminants. Its goal is to further our understanding of the complex microbial processes underlying environmental degradation, its detection and control, and ultimately, its prevention. Features new to this edition include: A completely new organization with topics such as pathogens in developing countries, effects of genetically modified crops on microbial communities, and transformations of toxic metals Comprehensive coverage of key topics such as bacteria in the greenhouse and low-energy waste treatment New coverage relating core book content to local, regional, and global environmental problems Environmental Microbiology, Second Edition is essential reading for environmental microbiologists and engineers, general environmental scientists, chemists, and chemical engineers who are interested in key current subjects in environmental microbiology. It is also appropriate as a textbook for courses in environmental science, chemistry, engineering, and microbial ecology at the advanced undergraduate and graduate levels.
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 2432 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Download or read book Applied and Environmental Microbiology written by and published by . This book was released on 2007 with total page 720 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biofilms in Plant and Soil Health written by Iqbal Ahmad and published by John Wiley & Sons. This book was released on 2017-10-02 with total page 556 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biofilms are predominant mode of life for microbes under natural conditions. The three-dimensional structure of the biofilm provides enhanced protection from physical, chemical and biological stress conditions to associated microbial communities. These complex and highly structured microbial communities play a vital role in maintaining the health of plants, soils and waters. Biofilm associated with plants may be pathogenic or beneficial based on the nature of their interactions. Pathogenic or undesirable biofilm requires control in many situations, including soil, plants, food and water. Written by leading experts from around the world, Biofilms in Plant and Soil Health provides an up-to-date review on various aspects of microbial biofilms, and suggests future and emerging trends in biofilms in plant and soil health. Issues are addressed in four sub areas: I) The fundamentals and significance of biofilm in plant and soil health, and the concept of mono and mixed biofilms by PGPR and fungal biofilms. II) Biochemical and molecular mechanisms in biofilm studies in plant associated bacteria, and techniques in studying biofilms and their characterization, gene expression and enhanced antimicrobial resistance in biofilms, as well as biotic and biotic factors affecting biofilm in vitro. III) The ecological significance of soil associated biofilms and stress management and bioremediation of contaminated soils and degraded ecosystems. IV) Pathogenic biofilm associated with plant and food and its control measures. This book is recommended for students and researchers working in agricultural and environmental microbiology, biotechnology, soil sciences, soil and plant health and plant protection. Researchers working in the area of quorum sensing, biofilm applications, and understanding microbiome of soil and plants will also find it useful.
Download or read book Recueil factice d articles de presse concernant l Op ra populaire en 1881 et en 1900 written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
