Download or read book Characterization of European Hazelnut Corylus Avellana Cultivars Using SSR Markers written by Tufan Gökirmak and published by . This book was released on 2005 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Microsatellite Marker Development Characterization and Mapping in European Hazelnut Corylus Avellana L and Investigation of Novel Sources of Eastern Filbert Blight Resistance in Corylus written by Gehendra Bhattarai and published by . This book was released on 2015 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: European hazelnut, an important nut crop in Oregon agriculture, is threatened by the fungal disease eastern filbert blight (EFB) caused by Anisogramma anomala (Peck) E. Müller. The hazelnut breeding program at OSU has been working on development of EFB-resistant cultivars. DNA markers allow mapping of traits using segregating progenies and identification of linked markers. The breeding of hazelnuts involves use of DNA markers to genotype individuals and facilitate selection in a procedure known as marker-assisted selection (MAS). This technique is particularly promising in tree crops with a long juvenile phase like the hazelnuts. 'Gasaway' resistance governed by a dominant allele at a single locus was identified and several EFB-resistant cultivars carrying the dominant allele from this source have been released. Infection of 'Gasaway' and some of its offspring by A. anomala isolates has been noted, and indicates an urgent need to investigate new sources of resistance, identify new resistance genes and incorporate them into the breeding effort. New microsatellite markers were developed from the genomic sequence of hazelnut cv. 'Jefferson'. A total of 17,802 simple sequence repeat (SSR) motifs exceeding 15 bp in length and consisting of tri-, tetra-, penta- and hexa- nucleotide repeats were identified in the 'Jefferson' genome. Removal of duplicates, short fragments, repeats at ends, and repeats containing only A's and T's reduced the number of unique fragments to 2,069. Visual inspection of these unique fragments alligned with genome sequences of 7 other accessions in Tablet software identified 489 sequences with variation in number of repeats but with conserved flanking regions. Amplification of a set of 24 diverse accessions and separation on agarose gels led to the development of 366 new polymorphic markers. These were used to genotype 48 diverse accessions using capillary electrophoresis for fragment sizing. An additional 15 parents were also fingerprinted at these new 366 microsatellite loci. Using the mapping population (OSU 252.146 x OSU 414.062), 213 loci were mapped. A dendrogram was constructed which showed a high level of polymorphism in hazelnut. Tightly linked markers were identified for 'Gasaway' resistance and MAS is routinely used. The hazelnut acreage in Oregon is increasing every year with the planting of these new EFB-resistant cultivars. However, concern about disease resistance breakdown prompted an investigation of 12 new EFB resistance sources. Segregating progenies were inoculated by placement of potted trees under an inoculation structure, in the greenhouse, or in the field by tying a diseased branch on each tree. A chi-square goodness-of-fit test was used compare observed with expected segregation ratios. Very few seedlings of Moscow selections N01, N01-06, and N01-07 were resistant and were likely escapes. 'Grand Traverse', C. heterophylla 'Ogyoo', 'Yoder #5', C. americana 'Rush', Moscow N23, N26, N27, and N37 segregated in 1:1 ratio, indicating resistance governed by a single locus and a dominant allele for resistance. 'Uebov' transmitted resistance to only ~15 % of its seedlings. Resistance from 'Uebov', 'Grand Traverse', and C. heterophylla 'Ogyoo' were assigned to LG6 but only 'Uebov' and 'Grand Traverse' were mapped. Resistance from 'Yoder #5', Moscow N27 and C. americana 'Rush' was assigned to LG7. Mapping of the remaining resistance sources will be completed in the future.

Download or read book DNA Markers and Characterization of Novel Sources of Eastern Filbert Blight Resistance in European Hazelnut Corylus Avellana L written by Brooke C. Peterschmidt and published by . This book was released on 2013 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: European hazelnut is a significant crop in the Pacific Northwest, and the US ranks 4th internationally for hazelnut production. Production in the Pacific Northwest is threatened, however, by the disease eastern filbert blight (EFB) caused by the fungus Anisogramma anomala (Peck) E. Müller. To meet the challenges faced by the hazelnut industry in Oregon and Washington, the breeding program at Oregon State University has focused on developing DNA marker technology and producing EFB resistant cultivars. This study focused on developing new microsatellite markers from hazelnut transcriptome sequences and on disease resistance from three accessions ('Culpla, ' 'Crvenje, ' and OSU 495.072) which showed no disease symptoms following a series of inoculations. DNA markers have been useful in hazelnut breeding for marker-assisted selection, construction of genetic linkage maps, cultivar fingerprinting, and phylogeny studies. Previously developed markers include AFLP, RAPD, ISSR, and microsatellite (SSR) markers developed from enriched libraries and ISSR fragments. This study utilized the transcriptome sequence from 'Jefferson' hazelnut to mine for microsatellites, align with the genomic sequence, design primers, screen for polymorphism, and characterize and map polymorphic markers. A total of 1432 microsatellites were mined from the transcriptome sequence, and the most frequently found motifs were AG (35.8%), AT (13.3%), and AAG (12.7%), and 382 primer pairs were designed. Screening showed that 119 markers were polymorphic, and these were characterized on sets of 50 and 14 accessions. Fifty-three markers that segregated in the mapping population or in three alternate populations were mapped and assigned to linkage groups. A dendrogram showed that accessions clustered mostly according to geographic origin. These results confirm the high level of diversity present in hazelnut, and the markers developed in this study will be useful for further genetics studies in hazelnut. The three EFB resistant parents 'Culpla, ' 'Crvenje, ' and OSU 495.072 were subjected to two inoculation treatments: greenhouse inoculations and exposure under an inoculation structure. The accessions remained free of disease after both treatments. Progeny segregating for resistance were produced. The progeny were inoculated either in the greenhouse or under the structure, and disease response recorded for each individual. DNA was extracted from seedlings, and sets of 32 seedlings from each resistant parent were screened with previously mapped markers using PCR and capillary electrophoresis. All three resistance sources were correlated with marker A614, allowing the resistance loci to be assigned to linkage group (LG) 6. The progeny were then screened with all known microsatellite markers on LG 6, and linkage maps constructed of the marker loci and resistance loci. Markers KG821, LG628, and LG696 are especially close to the resistance loci and will be useful for marker-assisted selection. Although these resistance loci are located in the same region of LG 6 as the 'Gasaway' resistance gene, they are different from 'Gasaway, ' and markers linked to resistance will be useful for introgressing and pyramiding resistance in new cultivars.

Download or read book Simple Sequence Repeat Marker Development and Use in European Hazelnut Corylus Avellana L written by Kahraman Gürcan and published by . This book was released on 2009 with total page 618 pages. Available in PDF, EPUB and Kindle. Book excerpt: New highly polymorphic simple sequence repeat (SSR) markers for European hazelnut (Corylus avellana L.). were developed from several sources. The markers were characterized, and segregation in a mapping population allowed to assign them to linkage groups. Their transferability across genera and species in the Betulaceae family was investigated. SSR markers were used to fingerprint several hazelnut accessions, including those from recent collections in northern Spain, Turkey, Georgia and Azerbaijan. A total of 170 new single-locus polymorphic SSR primers were developed in hazelnut: 76 from a library enriched for GA repeats, 13 from a library enriched for CA repeats, 23 from internal repeats in cloned inter simple sequence repeats (ISSR) fragments, 52 from repeats at the ends of ISSR fragments, and 6 from Betulaceae sequences in GenBank. These polymorphic loci were characterized using 50 hazelnut accessions, including the parents of a mapping population and 48 accessions that represented the high level of genetic diversity in European hazelnut. One hundred forty three of the new polymorphic SSR loci were mapped in a full-sib population. The map of the resistant parent consists of 344 markers (175 random amplified polymorphic DNA (RAPD) markers and 169 SSRs) spanning 837 cM with an average of 2.43 cM between markers, while the map of the susceptible parent consists of 366 markers (183 RAPDs and 183 SSRs) spanning 926 cM with an average of 2.53 cM between markers. SRR locus A601 co-segregated with the S-locus that controls pollen-stigma incompatibility, and four loci (B774, B720, B760, and SSR62) were tightly linked to the S-locus (_

Download or read book Advances in Plant Breeding Strategies Nut and Beverage Crops written by Jameel M. Al-Khayri and published by Springer Nature. This book was released on 2020-01-01 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book examines the development of innovative modern methodologies towards augmenting conventional plant breeding, in individual crops, for the production of new crop varieties under the increasingly limiting environmental and cultivation factors to achieve sustainable agricultural production, enhanced food security, in addition to providing raw materials for innovative industrial products and pharmaceuticals. This Volume 4, subtitled Nut and Beverage Crops, focuses on advances in breeding strategies using both traditional and modern approaches for the improvement of individual plantation crops. Included in Part I, eleven important nut species recognized for their economical and nutritional importance including Almond, Argan, Brazil nut, Cashew nut, Chestnut, Hazelnut, Macadamia, Peanut, Pine nut, Pistachio and Walnut. Part II covers two popular beverage species, coffee and tea. This volume is contributed by 53 internationally reputable scientists from 13 countries. Each chapter comprehensively reviews the modern literature on the subject and reflects the authors own experience.

Download or read book Recent advances in hazelnut corylus spp written by Valerio Cristofori and published by Frontiers Media SA. This book was released on 2023-02-08 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Plant Breeding Reviews Volume 45 written by Irwin Goldman and published by John Wiley & Sons. This book was released on 2021-10-21 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Breeding Reviews presents state-of-the-art reviews on plant genetics and the breeding of all types of crops by both traditional means and molecular methods. Many of the crops widely grown today stem from a very narrow genetic base; understanding and preserving crop genetic resources is vital to the security of food systems worldwide. The emphasis of the series is on methodology, a fundamental understanding of crop genetics, and applications to major crops.

Download or read book Genetic Diversity of Cultivated Tropical Plants written by Perla Hamon and published by Editions Quae. This book was released on 2003 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genomic Resource Development for European Hazelnut Corylus Avellana L written by Erik R. Rowley and published by . This book was released on 2016 with total page 303 pages. Available in PDF, EPUB and Kindle. Book excerpt: European hazelnut (Corylus avellana L.) is an important crop Oregon's Willamette Valley, producing 99% of the hazelnuts grown in North America and brings over US $60 million dollars to the region annually. Hazelnuts are rich in fiber and vitamins, as well in demand by consumers due to their popularity as the predominant flavor in a multitude of confectionary pastes and chocolate spreads. Breeding efforts are focused on developing hazelnut cultivars with enhanced agronomic traits of interest including size, blanching ability, and resistance to disease. Molecular markers have been developed for hazelnut and placed on a genetic linkage map, linking DNA marker sequences that segregate with phenotypic traits of interest. The objective of this study was to utilize highthroughput sequencing technologies to sequence the hazelnut genome, allowing for trait and marker discovery on a genome-wide scale. We have established genomic resources for hazelnut, including genomic and transcriptomic sequences to allow breeders the opportunity to exploit the wealth of genetic diversity when choosing germplasm for crosses. We chose the Eastern Filbert Blight (EFB) resistant diploid hazelnut cultivar 'Jefferson' (OSU 703.007) as the reference. The 'Jefferson' transcriptome assembly is represented by 28,255 transcript contigs, having an average length of 532bp, and an N50 of 961bp. These transcripts were characterized using both BLASTX protein homology and gene ontology (GO) classifications, with the majority of the predicted proteins to have high conservation with the most closely related plant sequences of Vitis, Populus, and Ricinus. A survey of gene classes enriched among tissue types further validates the assembly and transcript models. The draft genome assembly for 'Jefferson' was assembled de novo using Illumina short read technology into 36,641 contigs and scaffolds, with half of the assembly contained in scaffolds and contigs greater than 21.5 Kb. We captured approximately 91% (345 Mb) of the flow-cytometrydetermined genome size and identified 34,910 putative gene loci which were functionally annotated to identify candidates for future molecular validation. The majority of the annotated genes share homology with the best annotated and related genera Vitis, Prunus, Populus, and Ricinus. We also resequenced seven additional European hazelnut cultivars, detecting millions of variants between one of more of these genomes and that of 'Jefferson'. These variants were annotated based on the functional consequence each polymorphism on the affected loci. In addition, we utilized Genotyping-by-Sequence (GBS) technologies to produce a high-density genetic map within 138 individuals of an F1 hazelnut mapping population, representing a five-fold increase in marker density over the previous maps. Hazelnut genome sequencing has provided new resources to the scientific community and promises to accelerate trait discovery and enhance future breeding efforts, and serve as a tool for gene discovery and functional studies.

Download or read book Proceedings of the VIIth International Congress on Hazelnut written by L. Varvaro and published by . This book was released on 2009 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book United States Plant Patents written by United States. Patent and Trademark Office and published by . This book was released on 2014-10 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Globe Artichoke Genome written by Ezio Portis and published by Springer. This book was released on 2019-06-08 with total page 233 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the latest information on the genetics and genomics of the globe artichoke. It focuses on the latest findings, tools and strategies employed in genome sequencing, physical map development and QTL analyses, as well as genomic resources. The re-sequencing of four globe artichoke genotypes, representative of the core varietal types in cultivation, as well as the genotype of cultivated cardoon, has recently been completed. Here, the five genomes are reconstructed at the chromosome scale and annotated. Moreover, functional SNP analyses highlight numerous genetic variants, which represent key tools for dissecting the path from sequence variation to phenotype, as well as for designing effective diagnostic markers. The wealth of information provided here offers a valuable asset for scientists, plant breeders and students alike.

Download or read book The Use of Molecular and Biochemical Tools to Assist in the Breeding of Hazelnuts Corylus Spp written by Megan F. Muehlbauer and published by . This book was released on 2017 with total page 193 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hazelnuts rank 6th in world tree nut production, with approximately 800,000 metric tons produced per year. Commercial hazelnut production in the United States (the third largest producer of hazelnuts in the world) has been limited, due to the fungal pathogen Anisogramma anomala, the causal agent of eastern filbert blight (EFB). Interestingly, A. anomala is most deadly to the European hazelnut species (Corylus avellana), the only species used for commercial production, but is harbored by and does not cause symptoms in the native American species (C. americana). This fungal pathogen invades the vascular system of hazelnuts, girdles branches, and ultimately leads to death of the tree. Control measures to combat EFB are expensive and labor intensive, thus the most cost effective means of combating this disease is the use of disease resistant plant material. The Corylus genus holds 10 additional species, many of which carry EFB resistance. Over the past 15 years, extensive germplasm collection trips have been made to develop a broad hazelnut germplasm collection at Rutgers University, the entirely of which has been screened for resistance to EFB. The purpose of this study was to genetically characterize the novel collection of largely EFB resistant germplasm at Rutgers University using simple sequence repeat (SSR) markers. In addition, these same tools were used to further enhance the utility and better direct the use of this germplasm in the breeding program by performing a population structure analysis of A. anomala isolates collected from the United States and Canada. The final aspect of this dissertation investigates additional Corylus species by conducting a lipid content and profile analysis of four hazelnut species and interspecific hybrids to determine if there is a species effect on important kernel characteristics. Both the hazelnut germplasm collection and A. anomala isolate collection were found to be highly genetically diverse, and the analysis resolved 11 and 22 genetic populations, respectively. It was also found that the lipid content and profiles of hazelnuts will likely not be negatively affected by the introgression of different species into the breeding program. This work has demonstrated that there are a number of diverse sources of resistance in the Rutgers University hazelnut germplasm collection to the exceedingly genetically diverse fungus A. anomala, and introgression of sources of resistance in non C. avellana species will likely not effect commercially important kernel characteristics.

Download or read book Wild Crop Relatives Genomic and Breeding Resources written by Chittaranjan Kole and published by Springer Science & Business Media. This book was released on 2011-08-27 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: Wild crop relatives are now playing a significant part in the elucidation and improvement of the genomes of their cultivated counterparts. This work includes comprehensive examinations of the status, origin, distribution, morphology, cytology, genetic diversity and available genetic and genomic resources of numerous wild crop relatives, as well as of their evolution and phylogenetic relationship. Further topics include their role as model plants, genetic erosion and conservation efforts, and their domestication for the purposes of bioenergy, phytomedicines, nutraceuticals and phytoremediation. Wild Crop Relatives: Genomic and Breeding Resources comprises 10 volumes on Cereals, Millets and Grasses, Oilseeds, Legume Crops and Forages, Vegetables, Temperate Fruits, Tropical and Subtropical Fruits, Industrial Crops, Plantation and Ornamental Crops, and Forest Trees. It contains 125 chapters written by nearly 400 well-known authors from about 40 countries.

Download or read book Horticultural Plant Breeding written by Thomas J. Orton and published by Academic Press. This book was released on 2019-11-21 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: Horticultural Plant Breeding is a complete and comprehensive resource for the development of new cultivars or clones of horticultural crops. It covers the basic theories that underpin plant breeding and applies Mendelian, quantitative and population inheritance practices in smaller populations where the individual plant has high value. Specific traditional breeding methods are also covered, with an emphasis on how these methods are adapted for horticultural species. In addition, the integration of biotechnologies with traditional breeding methodologies is explored, with an emphasis on specific applications for fruits, vegetables and ornamental crop species. Presented in focused sections, Horticultural Plant Breeding addresses historical perspectives and context, and genetics as a critical foundation of plant breeding. It highlights treatments of the various components of breeding programs, such as breeding objectives, germplasm, population engineering, mating systems, enhanced selection methods, established breeding methods applicable to inbreeding and outcrossing situations, and post-breeding activities. Provides a complete and comprehensive resource for those involved in the development of new cultivars or clones of horticultural crops Guides readers to the most appropriate breeding strategy including potential integration of traditional and biotechnology strategies that will best achieve a cost-effective outcome Will include access to 20 narrated slide sets to facilitate additional understanding

Download or read book Statistical Methods for Tissue Culture Medium Optimization and A Multiplexed Fingerprinting Set for Hazelnuts written by Meleks̜en Akin and published by . This book was released on 2016 with total page 117 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hazelnut is one of the most important nuts in worldwide production and the European hazelnut (Corylus avellana L.) is the most economically important of the 11 recognized hazelnut species. Development of new cultivars is continuous, with emphasis on better nut quality, high yield and disease resistance. Hazelnuts are highly heterozygous, and clonally propagated. Traditional propagation methods in hazelnut are not rapid enough to provide the required nursery stock for newly released hazelnut cultivars, but micropropagation can provide rapid production of hazelnut planting stock. Several growth media are available for specific cultivars, but many are not suitable for the wide range of germplasm used in new cultivars. Micropropagation of hazelnuts remains challenging due to the various responses of diverse genotypes to in vitro growth. Several studies incrimentally improved the growth medium, but determining exact nutrient requirements was difficult. The aim of this study was to determine which statistical methods would make the growth medium optimization process more practical and to develop an optimal micropropagation medium for diverse hazelnuts by testing salts and ions as factors within the experimental design. In addition an SSR fingerprinting set suitable for a diverse group of hazelnuts was developed. The first study was designed to test the effect of salts on three hazelnut genotypes and compare two methods of data analysis. Driver and Kuniyuki Walnut medium (DKW) macro-salts (NH4NO3, Ca(NO3)2·4H2O, CaCl2·2H2O, MgSO4·7H2O, KH2PO4 and K2SO4) were varied from 0.5x to 3x DKW concentrations with 42 combinations in a IV-optimal design. Shoot quality, shoot length, multiplication and callus formation were rated and analyzed using Response Surface Methodology (RSM) and the Chi-Squared Automatic Interaction Detection (CHAID) data mining algorithm. Both analyses indicated that NH4NO3 was a predominant nutrient factor. RSM results were genotype dependent while CHAID included genotype as a factor in the analysis, allowing development of a common medium rather than several genotype-specific media. Overall, CHAID results were more specific and easier to interpret than RSM graphs. The optimal growth medium for diverse hazelnut genotypes was formulated as: 0.5× NH4NO3, 3x KH2PO4, 1.5x Ca(NO3)2 and and the rest of the macro salts set at 1x DKW with modified minor nutrients [4× H3BO3, 4x Na2MoO4·2H2O, 4x Zn(NO3)2·6H2O, 0.5× MnSO4·H2O, 0.5x CuSO4·5H2O]. The second study was to determine the effects of ions on tissue culture medium optimization. NH4+, Ca2+, Mg2+, SO42− and PO43− ions were used as factors in a D-optimal design. K+ and NO3− ions were used to bring the pH level to neutral, and as factors in the statistical analysis. The CHAID data mining algorithm was used to analyze shoot growth responses of three hazelnut genotypes. The algorithm trees revealed significant variables and their interactions, and provided exact cut-off amounts for each of the ions for the related growth response by incorporating genotype as an independent factor. The critical cut-off values for good shoot quality, elongation, multiplication and medium callus formation were suggested to be: NO3− 88 mM, NH4+

Download or read book Proceedings of the VIth International Congress on Hazelnut written by J. Tous and published by . This book was released on 2005 with total page 680 pages. Available in PDF, EPUB and Kindle. Book excerpt: