Download or read book Characterization of Essential Oils by Comprehensively Coupled Supercritical Fluid and Gas Chromatography SFGxGC written by Peter Ramashadi Makgwane and published by . This book was released on 2006 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Essential Oils by Comprehensively Coupled Supercritical Fluid and Gas Chromatography SFCxGC written by Peter Ramashadi Makgwane and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Essential oils are amongst the most complex samples an analyst can face in terms of the number of compounds involved. In many cases, minor components are of interest as they can impart a distinctive fragrance character to the oil. Because of the closely related structures and molecular weights among terpenes, positive identification of individual compounds is very difficult with a single chromatographic technique. Further, most of the analytical information is lost when a single technique is used because of the limited peak capacity and the resulting peak overlap. For many years, gas chromatography coupled to mass spectrometry (GC-MS) has been the benchmark technique for qualitative and quantitative analysis of essential oils. Retention indices and mass spectra have to be used in combination for confirmation of the identity of components in an essential oil. Other multidimensional or hyphenated techniques also offer advantages that aid in the identification of essential oil components. This thesis demonstrates the application of a comprehensively coupled supercritical fluid and fast temperature programmed gas chromatograph (SFCxGC) to the analysis of essential oils. An SFCxGC instrument was used to analyse the essential oils of Cymbopogon (lemongrass), Artemisia afra (wilde als), Tagetes minuta (kakiebos) and Pelargonium (geranium) species. The unique application of a porous layer open-tubular (PLOT) column, used in conjunction with supercritical carbon dioxide is demonstrated to effect group separation of polar, oxygenated compounds. This separation and elution of very polar compounds from a silica gel column is believed to occur due to the reduced phase ratio (??) of the system obtained by increasing the volume available to the mobile phase compared to that of a packed column. This separation obtained in the SFC is used to separate essential oils into different chemical classes such as non-polars, ethers, alcohols. Separated chemical classes are re-injected on-line by use of a modulator into a fast, second dimension, temperature programmed GC to effect separation of individual compounds based on their volatility. The entire sample is analysed by both the SFC and GC in such a way that the resolution obtained in the first dimension is conserved by the GC analyses. By using a range of standards, some of the peaks in these oils could be assigned. The identification of compounds was greatly aided by the combination of the two separation dimensions. The comprehensive two-dimensional technique arranges component peaks in a plane from which chemical class and volatility information of each component is readily obtained. The elution pattern within the two-dimensional chromatograms may also be used for direct comparison of oils without identification of the components in the essential oils.

Download or read book Analysis of Essential Oils by Gas Chromatography and Mass Spectrometry written by Yoshirō Masada and published by John Wiley & Sons. This book was released on 1976 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Essential Oils Analysis by Capillary Gas Chromatography and Carbon 13 NMR Spectroscopy written by V. Formáček and published by John Wiley & Sons. This book was released on 1982 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Capillary Gas Chromatography in Essential Oil Analysis written by Pat Sandra and published by . This book was released on 1987 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction to essential oil analysis. some aspects of essential oil preparation. considerations on the selection of cappilary columns for essential oil analysis. microtechniques in essential oil analysis. headscape versus classical analysis. Fingerprints in essential oil analysis. industrial quality control of essential oil by capillary GC. Retention indices in essential oil analysis. Possibilities and results of dual channel analysis of essential oils with fused silica capillaru columns. GC- mass specytrometry of essential oils: positive ion and negative ion and negative ion chemical ionazation techniques,computer matching techniques. Examples of artefact formation by chromatographic techniques. Possibilities, limitations, and future develpments in GC-FTIR analysis of essential oils. Possibilities of multidimensional GC in essential oils.

Download or read book The Analysis of Some South African Essential Oils by Comprehensive Two dimentional Gas Chromatography GCxGC written by Amanuel Zeru Zellelow and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: By virtue of their fragrance and therapeutic nature, essential oils have gained great application in the cosmetic and pharmaceutical industries. These oils, as products consumed by the public for the general health and cosmetics, should be of high quality and unadulterated. In this project comprehensive two-dimensional gas chromatography (GCxGC) was used for the qualitative analysis of some South African essential oils. The main purpose of the project was to evaluate GCxGC for identification of essential oil constituents and for fingerprinting the different essential oils for quality control purposes. Essential oils of the same kind but of different origin were compared and the quantitative variation between their components was studied. Gas chromatography-time-of flight mass spectrometry (GC-TOFMS) as well as comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GCxGC-TOFMS) was used to help identify component peaks separated in the GCxGC instrument. Once certain precautions are taken and the delicate modulator is correctly optimized, the GCxGC results are reproducible and easy to interpret. Overall, the GCxGC technique utilized showed good promise for quality control of essential oils.

Download or read book Analysis of Essential Oils by Combined Liquid solid and Gas liquid Chromatography written by Johannes J. Scheffer and published by . This book was released on 1978 with total page 127 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Comprehensive Two dimensional Gas Chromatography in Essential Oil Analysis written by Robert Allan Shellie and published by . This book was released on 2004 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Analysis of Some South African Essential Oils by Comprehensive Two dimensional Gas Chromatography GCxGC written by Amanuel Zeru Zellelow and published by . This book was released on 2003 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Supercritical Fluid Chromatography for Crude Oil Characterization written by Maurice Phillip Stadler and published by . This book was released on 1989 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: