Download or read book Characterization of the Redox and Proton mediated Chemistry of Engineered Electron Transfer Proteins written by Giulia Di Rocco and published by . This book was released on 2004 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Electron transfer Proteins written by Kathryn Duffy Bewley and published by . This book was released on 2013 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Electron-transfer proteins that are responsible for redox homeostasis and long-range electron transfer are vital to intracellular and extracellular processes. In this thesis, several examples of electron-transfer proteins are studied in order to determine the emergent properties of multi-electron transfer chemistry.Thioredoxin (Trx) is a small redox-active protein that functions via its disulfide bond. These disulfides, characterized by a CXXC motif, are found to have a range of redox potentials that are often linked to function. Chapter 2 uses a set of archaeal thioredoxins from Thermoplasma acidophilum and Archaeoglobus fulgidus to test the current hypotheses using protein film voltammetry and solution-based experiments that examine folding energies.Thioredoxin reductase (TrxR) functions to provide reducing equivalents to Trx to keep it active in the cell. The TrxR from Thermoplasma acidophilum has been noted to be unusual in that it does not use NADPH as a reductant, as found in most TrxRs. The reaction between T. acidophilum Trx and TrxR is explored in Chapter 3 and a bioinformatic analysis of Ta TrxR is included in Chapter 4 to better understand its relationship in the TrxR protein family, as well as attempt to identity its native reductant.In Chapter 5, the periplasmic decaheme cytochrome DmsE from Shewanella oneidensis is biochemically characterized. This protein is part of the dimethyl sulfoxide reduction pathway and is compared with MtrA, the well-studied decaheme protein from the dissimilatory metal reduction pathway in Shewanella. Additionally, a Cytoscape analysis of the MtrA/DmsE and OmcA protein families is presented.Finally, Chapter 6 explores the electrochemical properties of two multi-heme proteins from Nitrosomonas europaea: cytochrome c 554 and hydroxylamine oxidoreductase (HAO). Cytochrome c554, a tetraheme cytochrome, has been shown to have cooperativity between two of its heme groups and gating has been observed in protein film voltammetry (PFV) experiments. This gating is further explored in this Chapter. The enzymatic hydroxylamine reduction by HAO, a reverse reaction, is also presented.

Download or read book Characterization of Folding Dynamics and Accelerated Electron Transfer in Proteins written by and published by . This book was released on 2008 with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization Properties and Applications written by Tracey Rouault and published by Walter de Gruyter GmbH & Co KG. This book was released on 2017-08-21 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume on iron-sulfur proteins includes chapters that describe the initial discovery of iron-sulfur proteins in the 1960s to elucidation of the roles of iron sulfur clusters as prosthetic groups of enzymes, such as the citric acid cycle enzyme, aconitase, and numerous other proteins, ranging from nitrogenase to DNA repair proteins. The capacity of iron sulfur clusters to accept and delocalize single electrons is explained by basic chemical principles, which illustrate why iron sulfur proteins are uniquely suitable for electron transport and other activities. Techniques used for detection and stabilization of iron-sulfur clusters, including EPR and Mossbauer spectroscopies, are discussed because they are important for characterizing unrecognized and elusive iron sulfur proteins. Recent insights into how nitrogenase works have arisen from multiple advances, described here, including studies of high-resolution crystal structures.

Download or read book Protein Electron Transfer written by Dr Derek Bendall and published by Garland Science. This book was released on 2020-07-24 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is unique; the factual content and ideas it expounds are only just beginning to be touched upon in standard texts. Protein Electron Transfer is a major collaborative effort by leading experts and explores the molecular basis of the rapidly expan

Download or read book Strategies for Protein and Peptide Characterization and Quantification Using Electron transfer Dissociation Mass Spectrometry and Intrinsic Fluorescence written by and published by . This book was released on 2012 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: STRATEGIES FOR PROTEIN AND PEPTIDE CHARACTERIZATION AND QUANTIFICATION USING ELECTRON-TRANSFER DISSOCIATION MASS SPECTROMETRY AND INTRINSIC FLUORESCENCE Jason D. Russell Under the supervision of Associate Professor Joshua J. Coon At the University of Wisconsin-Madison The following chapters detail strategies for peptide and protein sequence analysis featuring electron-transfer dissociation (ETD) tandem mass spectrometry (MS/MS) and quantification using ultraviolet light-induced intrinsic fluorescence (UV-IF). Chapter 1 provides a brief background and overview. Chapter 2 discusses the optimization of the ETD MS/MS duty cycle for large-scale shotgun experiments. In Chapter 3, the first comprehensive analysis of peptide anions using negative ETD (NETD) is detailed. I report in Chapter 4 on the performance of an ion-ion reaction cell for intact protein analysis using large precursor populations for ETD MS/MS. The application of UV-IF for peptide detection and quantification using a custom fluorescence excitation and detection device is discussed in Chapter 5. An analysis of intact proteins from the 26S proteasome of Arabidopsis using top-down mass spectrometry and quantification by UV-IF is described in Chapter 6. In coordination with the Wisconsin Initiative for Science Literacy (WISL), Chapter 7 is a general description of my graduate research intended for non-specialists in an effort to promote science literacy in the broader community.

Download or read book Atomistic Approaches in Modern Biology written by Markus Reiher and published by Springer Science & Business Media. This book was released on 2007-01-08 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions by numerous experts

Download or read book Advancing Electron Transfer Dissociation Technologies for Characterization of Proteomes and Post translational Modifications written by Nicholas M. Riley and published by . This book was released on 2018 with total page 409 pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation presents research focusing on the development of new instrumentation and methodology to leverage ion-ion reactions for proteomic analyses. Electron transfer dissociation (ETD) technologies have proven a valuable alternative to collision-based fragmentation methods for sequencing peptides and proteins to advance global proteome characterization. Chapter 1 outlines the core concepts central to mass spectrometry (MS)-based proteomics, in addition to the basic principles of ETD and various strategies to improve its efficacy - including the technology that is the focus of this work, i.e., activated ion ETD (AI-ETD). Chapter 2 describes the first application of AI-ETD to intact proteins, which are more chemically complex and, thus, more difficult to sequence, than their peptide counterparts. Chapter 3 discusses a new strategy to improve signalto- noise in ETD spectra, which is especially beneficial for intact protein analysis and which has been incorporated into the newest generation of commercially available quadrupole-Orbitrap-linear ion trap hybrid MS systems. AI-ETD capabilities were also recently implemented on this stateof- the-art MS system (Chapter 4), and the ability to perform AI-ETD on this instrument enables comprehensive sequence coverage of moderately-sized intact proteins (Chapter 5), significantly improves proteoform characterization in large-scale analyses of complex mixtures of intact proteins (Chapter 6), and also enhances characterization of larger intact proteins (Chapter 7). Furthermore, AI-ETD improves characterization of post-translational modifications. Chapter 8 demonstrates the utility of AI-ETD for phosphosite localization in phosphopeptides and intact phosphoproteins, and Chapter 9 presents the largest glycoproteomic study to date by using AI-ETD to interrogate intact N-glycopeptides. Beyond positive-mode analyses of peptide and protein cations, ion-ion reactions also bring unique benefits to negative-mode analyses of precursor anions, where collision-based dissociation fails to consistently produce sequence-informative fragments. Chapter 10 describes implementation of negative ETD (NETD) and activated ion NETD (AI-NETD) and their application to whole-proteome sequencing in the negative mode, and Chapter 11 presents a modified search algorithm to improve interpretation of large-scale NETD and AI-NETD data. Conclusions and future directions of these projects are discussed in Chapter 12.

Download or read book Design and Characterization of Synthetic Electron Transfer Metalloproteins written by Gennady V. Kozlov and published by . This book was released on 1998 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Physical Characterization of Cytochrome C1 and a Lipoprotein Isolated from the Electron Transport System written by Daniel Pierre Gilboe and published by . This book was released on 1959 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Electron Transfer Activities Associated with Acetate Dependent Methanogenesis by Methanosarcina Barkeri MS written by John Michael Kemner and published by . This book was released on 1993 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Protein O GlcNAc Sites Using Electron transfer Dissociation Mass Spectrometry written by Namrata Dilip Udeshi and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biophysical Characterization of Bacterial Redox Proteins written by G̈ökçe Su Pulcu and published by . This book was released on 2011 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Electron transfer reactions are a key process in the energy cycle of many living organisms, such as bacteria or in the eukaryotic mitochondria. The transfer of electrons is achieved by a network of metalloproteins, which couple the delivery and receipt of electrons to catalytic transformations of biological chemistry. We investigated the redox chemistry of an electron shuttle, cytochrome c 554 , of the electron transfer of the protein. In addition to resolving all of the four heme potentials, we have also showed previously unobserved unidirectional electron transfer. While cytochrome c 554 is simply an electron shuttle, the diheme bacterial peroxidase uses the electrons for the catalysis of hydrogen peroxide that protects the cells against reactive oxygen species (ROS). The initial biophysical characterization of the Shewanella oneidensis peroxidase by UV/Visible and EPR spectroscopy and steady state kinetic investigations demonstrate that the formerly unstudied Shewanella enzyme can be classified among the traditional class of peroxidases, where an activation step is required prior to catalysis. Further kinetic investigation of the mechanism of Shewanella oneidensis C c P was carried out by rapid mixing stopped flow electronic absorption spectroscopy. Our results demonstrate the formation of two optically detectable intermediates in the mechanism. The second order rate constant for peroxide what is found to be 5.7x10 7 M -1 s -1 , which is similar to be the only available data from the Pseudomonas aeruginosa peroxidase. Redox coupled activation of the Shewanella peroxidase includes a loop motion near the peroxidatic active site. We have shown evidence for the conformational change in the loop by protein film voltammetry and MCD spectroscopy. The spectral assignments of the hemes are carried out and evidence of the six to five coordinate change at the active site heme is shown.

Download or read book Characterization and Reactivities of Iron sulfur Protein Active site Analogues written by John Gordon Reynolds and published by . This book was released on 1981 with total page 648 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biological Electron Transfer Chains Genetics Composition and Mode of Operation written by G.W. Canters and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: From May 3-7,1997, the NATO Advanced Research Workshop on 'Biological Electron Transfer Chains' was organized in Tomar, Portugal. In the application for support the choice of the topic was justified as follows: "[Until recently efforts] have concentrated on the study of the structure and function of individual redox enzymes and proteins. Enough information is now available to make a start with the study of biological electron transfer (E1) at the next higher level of organization, that of the complete ET chain." The interest in the workshop was high: the majority of participants had registered before the workshop was formally announced, which illustrates the popularity of the topic within the biochemical and biophysical communities. The present volume contains a number of reports based on the lectures presented by the key speakers during the meeting. The workshop dealt with the following three themes: a) Electron transfer, which is the subject of Chapter 1. The analysis of ET at the molecular level is still fundamental for an understanding of how ET chains operate in vivo. After 40 years of research the contours of the subject are becoming clear now. b) Bacterial redox chains. This is the subject of Chapter 2. Its contents show how complicated these chains can be, often involving a number of gene clusters. Our understanding of the regulatory aspects and control mechanisms of these chains is only in its beginning.

Download or read book Characterization of Redox and Gas responsive Heme thiolate Proteins written by Katherine A. Marvin and published by . This book was released on 2008 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Protein Electron Transfer written by Dr Derek Bendall and published by Garland Science. This book was released on 2020-07-24 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is unique; the factual content and ideas it expounds are only just beginning to be touched upon in standard texts. Protein Electron Transfer is a major collaborative effort by leading experts and explores the molecular basis of the rapidly expan