Download or read book Characterization and Nature of Seed Transmission of Seed Borne Virus Causing Mosaic of Cowpea Vigna Unguiculata L Walp written by SUMANA and published by . This book was released on 1991 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Sources of Resistance in Cowpea Vigna Unquiculata L Walp to Cowpea Aphid borne Mosaic and Cowpea Mosaic Viruses and Partial Characterization of a Potexvirus Affecting Bean and Cowpea Plates written by Monilola Arinola Taiwo and published by . This book was released on 1978 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Serological and Biological Characterization of Seed borne Isolates of Blackeye Cowpea Mosaic and Cowpea Aphid borne Mosaic Potyviruses in Vigna Unguiculata L Walp written by Muhammad Bashir and published by . This book was released on 1992 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Studies on a Seed transmissible Virus Causing Mosaic Symptoms in Cowpea Vigna Unguiculata L Walp from Ghana written by Lamptey, Paul N. L and published by 1972 [c1973]. This book was released on 1972 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Seed borne plant virus diseases written by K. Subramanya Sastry and published by Springer Science & Business Media. This book was released on 2013-01-05 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: Seeds provide an efficient means in disseminating plant virus and viroid diseases. The success of modern agriculture depends on pathogen free seed with high yielding character and in turn disease management. There is a serious scientific concern about the transmission of plant viruses sexually through seed and asexually through plant propagules. The present book provides the latest information along with the total list of seed transmitted virus and viroid diseases at global level including, the yield losses, diagnostic techniques, mechanism of seed transmission, epidemiology and virus disease management aspects. Additional information is also provided on the transmission of plant virus and virus-like diseases through vegetative propagules. It is also well known that seed transmitted viruses are introduced into new countries and continents during large-scale traffic movements through infected germplasm and plant propogules. The latest diagnostic molecular techniques in different virus-host combinations along with disease management measures have been included. The book shall be a good reference source and also a text book to the research scientists, teachers, students of plant pathology, agriculture, horticulture, life sciences, green house managers, professional entrepreneurs, persons involved in quarantines and seed companies. This book has several important features of seed transmitted virus diseases and is a good informative source and thus deserves a place in almost all university libraries, seed companies and research organizations.

Download or read book Characterization of a Mosaic Virus Disease Occurring on Cowpea Vigna Unguiculata L Walp written by B. Mahalakshmi and published by . This book was released on 2005 with total page 92 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization and Serodiagnosis of a Cowpea Vigna Unguiculata L Walp Mosaic Virus written by SANJEEVA REDDY H and published by . This book was released on 1997 with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification of Genotypes and Tagging of Available SSR Markers Linked to BCMV Bean Common Mosaic Virus Resistance in Early Segregating Generation of Cowpea Vigna Unguiculata L Walp and Evaluation of Nature of Seed Transmission written by P. Aishwarya and published by . This book was released on 2017 with total page 80 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization and Seed Storage Studies of Cowpea Vigna Unguiculata L Walp Genotypes written by B.H Sunitha and published by . This book was released on 2008 with total page 105 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characteristics of the Lentil Strain of Pea Seedborne Mosaic Virus written by Jody Jellison Goodell and published by . This book was released on 1983 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: The lentil strain of pea seedborne mosaic virus, PSbMV-L, was distinguished from the standard isolates of PSbMV by a natural inoculum reservoir in lentil seed and by separate resistance genes in Pisum and Lens. PSbMV-L was intermediate in symptom severity between the standard and New Zealand isolates of PSbMV. Seed transmission rates up to 16.7% were observed for PSbMV-L in seed increase plots of infected lentil accessions, but the virus was not detected in commercial lentil plantings. The virus was nonpersistently transmitted by Acyrthosiphon pisum. Maximum rates of aphid transmission occurred with acquisition access periods of 3 to 5 minutes and ranged from 80 to 100%. Aphid transmission rates decreased with increasing temperature. Apparent trimodal transmission of PSbMV-L was observed. PSbMV-L is characterized by intrinsic particle instability. Partial purification was obtained by extracting infected lentil leaves in a neutral buffer, clarification with 7.5% chloroform, centrifugation through PEG (6 %)- sucrose (35%) cushions and equilibrium centrifugation in CsC1 gradients. The absorbance ratio 260/280 was 1.15. PSbMV-SL25 and PSbMV-L were indistinguishable on the basis of protein subunit molecular weight and could not be differentiated using classical serological techniques. The strains could be separated by quantitative ELISA using polyclonal sera produced in rabbit or chicken. Attempts to achieve greater antibody specificity were made with spleen cells from previously immunized mice that were fused with myoloma cells to create antibody-secreting hybridomas. The resultant colonies were screened using a modified ELISA against PSbMV-SL25 and PSbMV-L for the presence of specific antibody production. Selected colonies were expanded. The molecular weight of PSbMV-L RNA was estimated at 3.5 million. DNA complementary to PSbMV-C4 RNA was prepared using the random primer method of Taylor et al. Hybridization kinetics with PSbMV-L, PSbMV-SL25 and BYMV-Gil RNA were studied using Sl-nuclease assays. The lentil strain showed significantly lower levels of hybridization than the homologous control. Heterologous hybridization was severely decreased when the stringency of the assay conditions was increased.

Download or read book Studies on a Seed transmissible Virus Causing Mosaic Symptoms in Cowpea Vigna Amguiculata L Walp from Ghana written by Paul Nii Lante Lamptey and published by . This book was released on 1972 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of the Viral Proteins Involved in the RNA Replication of Cowpea Mosaic Virus written by Hans van Bokhoven and published by . This book was released on 1993 with total page 111 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Partial Characterization of the P4 Pathotype of Pea Seedborne Mosaic Virus written by Paul D. Kohnen and published by . This book was released on 1992 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological, serological, and molecular characteristics of the P4 pathotype of pea seedborne mosaic potyvirus (PSbMV) were investigated. The characterization allowed P4 to be differentiated from other pathotypes of the same virus. P4 was distinguished from P1 on the basis of host symptomatology. It induced varied, but often distinctive symptoms in systemically-infected pea (Pisum sativum) cultivars. P4 inoculation of indicator hosts, notably Chenopodium album, resulted in the formation of local lesions. Pathotype P4 was seed transmitted at low frequencies relative to P1. In ten selected pea cultivars, seed transmission of P4 never exceeded 0.7%, while P1 was transmitted at frequencies as high as 33%. In mixed infections with P1, pathotype P4 was seed transmitted at 0- 2% as determined by P4-specific indirect ELISA. However, limited polymerase chain reaction (PCR) data suggest that P4 may be transmitted with P1 at higher rates than indicated by ELISA data. P4 was also aphid transmitted at relatively low rates. Pea aphids (Acyrthosiphon pisum) allowed 3 or 5 min acquisition access periods (AAP's) transmitted P4 at a maximum rate of 16% (3 aphids/plant). P4 transmission required short (1-7 min) AAP's and exhibited no bimodal character within tested parameters. P4 could be serologically distinguished from the other pathotypes using monoclonal and polyclonal antibodies. Tests with cross-reactive P4 antiserum suggest that P4 is more closely related to P2 than P1. P4-specific antiserum was derived by cross-absorbing diluted antiserum with P1- infected pea sap to eliminate P1- reactive antibodies. P1 and P4 RNA was detected and differentiated using pathotype-specific oligonucleotide primers in conjunction with PCR. Viral RNA sequences were amplified in pure preparations as well as crude pea tissue (leaf, root, pollen, and seed) extracts.

Download or read book Studies on Transmission of Pea Seed borne Mosaic Virus by Aphids and the Mechanism of Transmission written by Wa-lee Lim and published by . This book was released on 1976 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book M moire l eminentissime et S r nissime College Electoral written by and published by . This book was released on 1711 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Legume Research written by and published by . This book was released on 1990 with total page 846 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Seed Transmission Host Range Purification and Properties of Pea Seed borne Mosaic Virus written by John Edward Knesek and published by . This book was released on 1973 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt: